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- 저자 : Mei-Ling Xu (검색결과 30 건)
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Xu, Mei Ling,Kim, Seung Cheol,Kim, Hyoung Jin,Ju, Woong,Kim, Yun Hwan,Kim, Hong-Jin Elsevier 2017 Protein expression and purification Vol.132 No.-
<P><B>Abstract</B></P> <P>Human papillomavirus (HPV) E6 protein is an oncoprotein with a pivotal role in cervical carcinogenesis. Expression and purification of HPV E6 from <I>Escherichia coli</I> (<I>E. coli</I>) has been difficult because of its strong hydrophobicity even when expressed as a fusion protein with glutathione S-transferase (GST). There has been no protocol suggested for purifying GST-tagged HPV E6 protein with high purity so far. Herein, we provide efficient protocol for purifying GST-HPV16 E6 protein for the first time. In the current study, the GST-tagged protein was expressed in <I>E. coli</I> and a purification method was designed using cation-exchange chromatography followed by GST-affinity chromatography. Using physiological pH buffer during cell lysis and first cation-exchange chromatography significantly reduced yield of full-length GST-HPV16 E6 protein. It was found that using an alkaline buffer during cation-exchange chromatography was needed to obtain full length GST-HPV16 E6 protein. GST-HPV16 E6 protein recovered from the purification using alkaline condition retained its inherent p53-binding ability. Moreover, we were able to detect anti-HPV16 E6 antibodies with high sensitivity in sera from patients with cervical cancer using the GST–HPV16 E6 protein. It was found that the GST-HPV16 E6 protein could be used as a coating agent to enhance the sensitivity of detection of serum anti-HPV16 E6 antibodies when treated with ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N'-tetraacetic acid (EGTA). These results indicate that the two-step chromatographic purification allows obtaining high purity of GST-HPV16 E6 protein and the GST-HPV16 E6 is suitable to be used as an antigen of serology assay.</P> <P><B>Highlights</B></P> <P> <UL> <LI> High purity of GST-HPV16 E6 was successfully purified under alkaline condition. </LI> <LI> GST-HPV16 E6 purified under alkaline condition has ability for p53 binding. </LI> <LI> EGTA treatment to GST-HPV16 E6 increases reactivity towards anti-HPV16 E6 antibody. </LI> </UL> </P>
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Ling-fen Xu,Cheng Xu,Zhi-Qin Mao,Xu Teng,Li Ma,Mei Sun 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.2
To explore whether platelet-activating factor (PAF) can disrupt the intestinal epithelial barrier directly and is associated with structural alterations of the F-actin-based cytoskeleton, and to observe the protective effect of intestinal trefoil factor (ITF), we establish an intestinal epithelia barrier model using Caco-2 cells in vitro. Transepithelial electrical resistance and unidirectional flux of lucifer yellow were measured to evaluate barrier permeability; immunofluorescent staining and flow cytometry were applied to observe morphological alterations and to quantify proteins of the F-actin cytoskeleton: the tight junction marker ZO-1 and Claudin-1 were observed using immunofluorescent staining. PAF significantly increased paracellular permeability, at the same time, F-actin and tight junction proteins were disrupted. It was thought that ITF could reverse the high permeability by restoring normal F-actin, ZO-1 and Claudin-1 structures. These results collectively demonstrated that PAF plays an important role in the regulation of mucosal permeability and the effects of PAF are correlated with structural alterations of the F-actin-based cytoskeleton and of tight junctions. ITF can protect intestinal epithelium against PAF-induced disruption by restricting the rearrangement of the F-actin cytoskeleton and of tight junctions.
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Xu, Mei Ling,Kim, Hyoung-Jin,Choi, Yoo-Ri,Kim, Hong-Jin The Korean Society of Ginseng 2012 Journal of Ginseng Research Vol.36 No.4
Vaccination is the main strategy for preventing influenza infection. However, vaccine efficacy is influenced by several factors, including age and health status. The efficacy of the influenza vaccine is much lower (17% to 53%) in individuals over 65 yr of age compared with young adults (70% to 90%). Therefore, increasing vaccine efficacy remains a challenge for the influenza vaccine field. In this study, we investigated the impact of supplementing vaccination with the dietary intake of Korean red ginseng (RG) extract and RG saponin. Mice were immunized two times intranasally with inactivated influenza A (H1N1) virus. Mice received RG extract or RG saponin orally for 14 d prior to the primary immunization. After the primary immunization, mice continued to receive RG extract or RG saponin until the secondary immunization. Mice vaccinated in combination with dietary intake of RG extract and RG saponin showed elevated serum anti-influenza A virus IgG titers and improved survival rates in lethal influenza A virus infection: 56% and 63% of mice receiving RG extract or RG saponin survived, respectively, while 38% of mice that only received the vaccine survived. Moreover, mice receiving RG extract supplementation recovered their body weight more quickly than those not receiving RG extract supplementation. We propose that the dietary intake of RG extract and RG saponin enhances the vaccine-induced immune response and aids in providing protection against influenza virus infection.
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Mei Ling Xu,김형진,장돈용,김홍진 한국미생물학회 2013 The journal of microbiology Vol.51 No.3
Acidic protein levels in the milk decrease markedly as lactation progresses, suggesting that it is an important part of the colostrum. However, little attention has been paid to their biological function. In this study, we isolated the acidic protein fraction of bovine colostrum (AFC, isoelectric point <5) by anion-exchange chromatography, and investigated the effect of its dietary intake on influenza A (H1N1) virus infection. 100% of mice infected with 1 LD50 of the virus survived when administered AFC for 14 days prior to infection, compared with 33% survival when administered phosphate buffered saline (PBS). Moreover, consumption of AFC reduced the weight loss associated with infection. We propose that dietary intake of AFC has a prophylactic effect on influenza A virus infection.
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Mei Ling Xu,김형진,김홍진 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.4
Previous studies have revealed that ingestion ofbovine colostrum is effective in preventing pathogens frominvading through the gastrointestinal tract (GI) and modulatingthe mucosal immunity of the GI tract, indicatingthat its effect is principally local. Thus it is unclear ifingestion of bovine colostrum can affect the systemicimmune system. In this study, we investigated the effect oftaking bovine colostrum (vs phosphate-buffered saline) for14 days on the behavior of the immune cells of mice. Isolated splenocytes, which are pivotal cells of systemicimmunity, were then stimulated with Escherichia colilipopolysaccharide. Bovine colostrum significantly reducedNK cell and monocyte activities and lymphoproliferaltiveresponses to LPS stimulation. Thus dietary bovine colostrumrenders immune cells less responsive to LPS stimulation. Dietary bovine colostrum thus affects the systemicimmune system and may have anti-inflammatory actions.
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Xu, Mei Ling,Kim, Hyoung Jin,Kim, Hong-Jin 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.4
Previous studies have revealed that ingestion of bovine colostrum is effective in preventing pathogens from invading through the gastrointestinal tract (GI) and modulating the mucosal immunity of the GI tract, indicating that its effect is principally local. Thus it is unclear if ingestion of bovine colostrum can affect the systemic immune system. In this study, we investigated the effect of taking bovine colostrum (vs phosphate-buffered saline) for 14 days on the behavior of the immune cells of mice. Isolated splenocytes, which are pivotal cells of systemic immunity, were then stimulated with Escherichia coli lipopolysaccharide. Bovine colostrum significantly reduced NK cell and monocyte activities and lymphoproliferaltive responses to LPS stimulation. Thus dietary bovine colostrum renders immune cells less responsive to LPS stimulation. Dietary bovine colostrum thus affects the systemic immune system and may have anti-inflammatory actions.
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Mei Ling Xu,Hyoung Jin Kim,Yoo Ri Choi,Hong-Jin Kim 고려인삼학회 2012 Journal of Ginseng Research Vol.36 No.4
Vaccination is the main strategy for preventing infl uenza infection. However, vaccine effi cacy is infl uenced by several factors, including age and health status. The effi cacy of the infl uenza vaccine is much lower (17% to 53%) in individuals over 65 yr of age compared with young adults (70% to 90%). Therefore, increasing vaccine effi cacy remains a challenge for the infl uenza vaccine fi eld. In this study, we investigated the impact of supplementing vaccination with the dietary intake of Korean red ginseng (RG) extract and RG saponin. Mice were immunized two times intranasally with inactivated infl uenza A (H1N1) virus. Mice received RG extract or RG saponin orally for 14 d prior to the primary immunization. After the primary immunization, mice continued to receive RG extract or RG saponin until the secondary immunization. Mice vaccinated in combination with dietary intake of RG extract and RG saponin showed elevated serum anti-infl uenza A virus IgG titers and improved survival rates in lethal infl uenza A virus infection: 56% and 63% of mice receiving RG extract or RG saponin survived, respectively, while 38% of mice that only received the vaccine survived. Moreover, mice receiving RG extract supplementation recovered their body weight more quickly than those not receiving RG extract supplementation. We propose that the dietary intake of RG extract and RG saponin enhances the vaccine-induced immune response and aids in providing protection against infl uenza virus infection.
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Mei Ling Xu,김형진,Ga Ram Wi,김홍진 한국미생물학회 2015 The journal of microbiology Vol.53 No.9
Human respiratory syncytial virus (hRSV) is the most common cause of respiratory tract infection among young children because of immature T cell immunity of them against hRSV. CD8 T cells play a pivotal role in clearing hRSV and preventing subsequent infection. We examined the effects of dietary bovine colostrum on virus infection and CD8 T cell responses following hRSV infection in the mouse model. Mice received bovine colostrum for 14 days prior to hRSV challenge, and lung indexes (severity of symptom) and lung virus titers were analyzed. In addition, the activation of CD8 T cells in the bronchoalveolar lavage fluids (BALFs) of mice receiving bovine colostrum were compared with those in the BALFs of mice receiving phosphate-buffered saline (PBS) or ribavirin, post virus challenge. The severity of infection and lung virus titers were reduced in the mice receiving bovine colostrum, compared to those receiving PBS. Moreover CD8 T cell responses were selectively enhanced in the former. Our results suggest that dietary bovine colostrum exerts the effects to inhibit hRSV and ameliorate the symptom by hRSV infection, and enhances the CD8 T cell response during the hRSV infection.
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『중국어회화전서(中國語會話全書)』의 중(中),한(韓) 역음(譯音) 표기 연구
서미령 ( Mei Ling Xu ) 연세대학교 인문학연구원 2015 人文科學 Vol.105 No.-
This study is about the ZHONG GUO YU HUI HUA QUAN SHU by ZHANG ZHI YING, which haven’t been studied before. This study will focus on the pronunciations of the Chinese initials and finals, Hangeul translation notes, in this Chinese textbook. Consonant phonetic features are as followed, ① A form of ‘ZHONG JIAN YIN’ was used. ② A form of ‘ㅇ+phoneme’. ③ The ‘r’ was indicated as /ㅿ/, The ‘f’ was indicated as /ㅸ/, The ‘ㅣ’ was indicated as /□/. Collection of phonetic features are as followed. ① ‘i’ was referred to as /ㅣ/, [□] was referred to as /ㅡ/, and [□] was referrred to as /ㅢ/. ② ‘ua’ was referred to as /□/, ‘ue’ was referred tp as /□/ . The textbooks that were published during the period of Japanese colonial era reflect the languages of the transition period in modern times. They could be evidential materials on the history of Chinese used outside of China. This study will contribute to the research on the Beijing sounds since this textbook ZHONG GUO YU HUI HUA QUAN SHU was published in Mandarin.
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