Identification of a Novel Cassette Array in Integron-bearing Helicobacter Pylori Strains Isolated from Iranian Patients

Goudarzi, Mehdi,Seyedjavadi, Sima Sadat,Fazeli, Maryam,Roshani, Maryam,Azad, Mehdi,Heidary, Mohsen,Navidinia, Masoumeh,Goudarzi, Hossein Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.7

Helicobacter pylori as the second most common cause of gastric cancer in the world infects approximately half of the developed countries population and 80% of the population living in developing countries. Integrons as genetic reservoirs play major roles in dissemination of antimicrobial resistance genes. To the best of our knowledge, this is the first study to report carriage of class 1 and 2 integrons and associated gene cassettes in H. pylori isolates from Iran. This cross-sectional study was conducted in Tehran among 110 patients with H. pylori infection. Antimicrobial susceptibility testing (AST) for H. pylori strains were assessed by the micro broth dilution method. Class 1 and 2 integrons were detected using PCR. In order to determine gene cassettes, amplified fragments were subjected to DNA sequencing of both amplicon strands. The prevalence of resistance to clarithromycin, metronidazole, clarithromycin, tetracycline, amoxicillin, rifampin, and levofloxacin were 68.2% (n=75), 25.5% (n=28), 24.5% (n=27), 19.1% (n=21), 18.2% (n=20) and 16.4% (n=18), respectively. Frequency of multidrug resistance among H. pylori isolates was 12.7%. Class 2 integron was detected in 50 (45.5%) and class 1 integron in 10 (9.1%) H. pylori isolates. The most predominant gene cassette arrays in class 2 integron-bearing H. pylori were included sat-era-aadA1, dfrA1-sat2-aadA1, blaoxa2 and, aadB whereas common gene cassette arrays in class 1 integron were aadB-aadA1-cmlA6, aacA4, blaoxa2, and catB3. The high frequency of class 2 integron and multidrug resistance in the present study should be considered as a warning for clinicians that continuous surveillance is necessary to prevent the further spread of resistant isolates.

Genotyping of Peroxisome Proliferator-Activated Receptor gamma in Iranian Patients with Helicobacter pylori Infection

Goudarzi, Hossein,Seyedjavadi, Sima Sadat,Fazeli, Maryam,Azad, Mehdi,Goudarzi, Mehdi Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13

Helicobacter pylori (H. pylori) infection as a serious problem in both adults and children can induce chronic gastritis, peptic ulcer disease (PUD), and possibly gastric cancer. The aim of the current study was to survey antibiotic resistance and also to determine influence of PPAR$\gamma$ polymorphism in patients with H. pylori infection. During an 11-month-period, 98 H. pylori isolates were collected from 104 biopsy specimens. In vitro susceptibility of H. pylori isolates to 4 antimicrobial agents metronidazole, clarithromycin, amoxicillin and tetracycline were assessed by quantitative method according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) guideline. PPAR$\gamma$ polymorphism was determined using polymerase chain reaction-restriction fragment length polymorphism assay. The frequency of H. pylori infection in our study was 94.2%. In vitro susceptibility data showed that highest level of resistance was related to metronidazole (66.3%), and the majority of H. pylori isolates were highly susceptible to amoxicillin and tetracycline (94.9% and 96.9%, respectively). Genotypic frequencies were 25.5% for CC (Pro12Pro), 40.8% for GC (Pro12Ala) and 33.7% for GG (Ala12Ala). In our study, CG genotype had highest distributions among infected patients with H. pylori. The study suggests that the PPAR-$\gamma$ Pro12Ala polymorphism could be evaluated as a potential genetic marker for susceptibility to gastric cancer in the presence of H. pylori infection.

Genetic Variability of Methicillin Resistant Staphylococcus Aureus Strains Isolated from Burns Patients

Mehdi Goudarzi,Nobumichi Kobayashi,Maryam Fazeli,Masoumeh Navidinia,Ali Hashemi 질병관리본부 2019 Osong Public Health and Research Persptectives Vol.10 No.3

Objectives: Staphylococcus aureus is a nosocomial pathogen that provides a major challenge in the healthcare environment, especially in burns units where patients are particularly susceptible to infections. In this study, we sought to determine molecular types of S. aureus isolates collected from burns patients, based on staphylococcal protein A and coagulase gene polymorphisms. Methods: Antibiotic susceptibility testing of 89 S. aureus strains isolated from burn wounds of patients was assessed using the Kirby-Bauer disk diffusion method. Strains were characterized by spa typing, coa typing, and resistance and toxin gene profiling. Results: A total of 12 different spa types were identified with the majority being t790 (18%). Panton- Valentine leucocidin encoding genes were identified in spa types t044 (5.6%), t852 (2.2%) and t008 (2.2%). The most commonly detected antibiotic resistance gene was ant (4΄)-Ia (60.7%). Ten different coa types were detected and the majority of the tested isolates belonged to coa III (47.2%). All the high-level mupirocin-resistant and low-level mupirocin resistant strains belonged to coa type III. Conclusion: The present study illustrated that despite the high frequency of coa III and spa t790 types, the genetic background of S. aureus strains in Iranian burns patients was diverse. The findings obtained are valuable in creating awareness of S. aureus infections within burns units.

Microchips and their Significance in Isolation of Circulating Tumor Cells and Monitoring of Cancers

Sahmani, Mehdi,Vatanmakanian, Mousa,Goudarzi, Mehdi,Mobarra, Naser,Azad, Mehdi Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

In micro-fluid systems, fluids are injected into extremely narrow polymer channels in small amounts such as micro-, nano-, or pico-liter scales. These channels themselves are embedded on tiny chips. Various specialized structures in the chips including pumps, valves, and channels allow the chips to accept different types of fluids to be entered the channel and along with flowing through the channels, exert their effects in the framework of different reactions. The chips are generally crystal, silicon, or elastomer in texture. These highly organized structures are equipped with discharging channels through which products as well as wastes of the reactions are secreted out. A particular advantage regarding the use of fluids in micro-scales over macro-scales lies in the fact that these fluids are much better processed in the chips when they applied as micro-scales. When the laboratory is miniaturized as a microchip and solutions are injected on a micro-scale, this combination makes a specialized construction referred to as "lab-on-chip". Taken together, micro-fluids are among the novel technologies which further than declining the costs; enhancing the test repeatability, sensitivity, accuracy, and speed; are emerged as widespread technology in laboratory diagnosis. They can be utilized for monitoring a wide spectrum of biological disorders including different types of cancers. When these microchips are used for cancer monitoring, circulatory tumor cells play a fundamental role.

Microstructure, Mechanical Properties and Wear Performance of WC/Brass Composites Produced by Pressureless and Spark Plasma Sintering Processes

Mehdi Naghikhani,Mohammad Ardestani,Mohammad Moazami‑Goudarzi 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.6

Tungsten carbide reinforced brass matrix composites with 30 and 70 wt% brass were synthesized by mechanical milling anddensification of brass/tungsten carbide powder mixtures. Two different sintering methods included pressureless and sparkplasma sintering were applied for densification of the milled powders. The relative density of the spark plasma sinteredsamples was much higher than those of the pressureless sintered ones confirming higher microstructural densification duringspark plasma sintering. The hardness of the spark plasma sintered composites containing 30 and 70 wt% brass were 303 and175 Vickers, respectively which was about five times more than those of the pressureless sintered composites with similarweight percent of brass. In addition, the wear performance of the composites was investigated by the pin on disc method. The spark plasma sintered samples with 30 wt% brass showed the highest wear resistant among the processed samplesowing to their higher hardness. The microscopic observations revealed that delamination and adhesion were the dominantwear mechanisms for the pressureless and spark plasma sintered composites, respectively. The bending strength of the sparkplasma sintered samples with 30 and 70 wt% brass were 329 and 453 MPa, respectively.

Landfill Site Selection using GIS and AHP: a Case Study: Behbahan, Iran

Zeinab Ghaed Rahmat,Mehdi Vosoughi Niri,Nadali Alavi,Gholamreza Goudarzi,Ali Akbar Babaei,Zeinab Baboli,Mohsen Hosseinzadeh 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.1

Finding a suitable site to dispose solid waste is a difficult task for municipality because it is necessary to consider the different factors and criteria in the landfill siting process. In this study, in order to consider all parameters, a combination of Geographic Information System (GIS) and the Analytic Hierarchy Process (AHP) was used for landfill site selection. For the purpose of making decisions in landfill site selection a hierarchy structural was formed and different parameters have been identified, including distance to groundwater, distance to surface water, sensitive ecosystems, land cover, distance to urban and rural areas, land uses, distance to roads, slope, soil type and distance to waste generation places. At first, the rating method was used to evaluate each criterion individually. Then, the relative importance of criteria to each other was determined by an analytic hierarchy process (AHP). Simple Additive Weighting (SAW) method was applied to evaluate the land suitability. The results showed that 38% of the study area have high suitability for land filling. Finally, five sites were a candidate for field investigation with more details.

Efficient Routing using Partitive Clustering Algorithms in Ferry-based Delay Tolerant Networks

Behrouz Jedari,Rouhollah Goudarzi,Mehdi Dehghan 보안공학연구지원센터 2009 International Journal of Future Generation Communi Vol.2 No.4