Low molecular fucoidan and its macromolecular complex with bee venom melittin

Mavlonov, G. T.,Lee, J. M,Shin, Lee,Yi, T. H.,Abdurakhmonov, I. Y. 'Scientific Research Publishing, Inc.' 2011 Advances in bioscience and biotechnology Vol.2 No.4

Development of superparamagnetic iron oxide nanoparticles via direct conjugation with ginsenosides and its in-vitro study

Singh, Hina,Du, Juan,Singh, Priyanka,Mavlonov, Gafurjon Tom,Yi, Tae Hoo Elsevier 2018 Journal of photochemistry and photobiology. B, Bio Vol.185 No.-

<P><B>Abstract</B></P> <P>The current study focused on direct conjugation of superparamagnetic iron oxide nanoparticles (SPIONs) with ginsenosides CK and Rg3. The direct conjugation approach was low-cost, eco-friendly, simple, fast and high yield. The synthesized conjugates (SPION-CK and SPION-Rg3) were characterized by field emission transmission electron microscopy, dynamic light scattering, zeta potential, X-ray diffractometer, and magnetometer. The characterization results confirmed the formation of SPIONs conjugates. The maximum attaching percentage for ginsenosides to SPIONs was found to be 5%. In vitro cytotoxicity assay in HaCaT keratinocyte cells revealed that the conjugates were non-cytotoxic to normal cells. Moreover, the anti-inflammatory activity of SPION-CK and SPION-Rg3 were investigated. The expression of reactive oxygen species (ROS) in lipopolysaccharide-activated RAW 264.7 (murine macrophage cells) were inhibited by SPIONs conjugates in a dose-dependent manner. In addition, SPION-CK and SPION-Rg3 significantly reduced the production of nitric oxide and inducible nitric oxide synthase (iNOS) in a dose-dependent manner in the lipopolysaccharide-induced RAW 264.7 cells. Overall the results suggested that the SPIONs were conjugated with ginsenosides CK and Rg3 by using direct conjugation approach were non-cytotoxic and can be used as a carrier for intracellular release of ginsenosides in inflammatory diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Simple, cheap, ecofriendly and high yield direct conjugation method has been developed. </LI> <LI> Direct conjugation method was used for superparamagnetic iron oxide nanoparticles (SPIONs) conjugation. </LI> <LI> Direct conjugation method was successful in conjugating SPIONs with ginsenosides CK and Rg3. </LI> <LI> The ginsenosides conjugates were nontoxic to HaCaT keratinocyte cells. </LI> <LI> The conjugates showed anti-inflammatory effects in RAW 264.7 cells. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>Conjugation scheme of ginsenosides with superparamagnetic iron oxide nanoparticles (SPIONs).</P> <P>[DISPLAY OMISSION]</P>

Microbial Conversion of Major Ginsenoside Rb1 to Minor Ginsenoside C-K by Flavobacterium sp. THG-176 Isolated from Pine Field Soil

Ji-Yeon SHIN,Sang-Yong PARK,Gafurjon Mavlonov,Eun-Hee LEE,Jung-Min LEE 고려인삼학회 2011 고려인삼학회 학술대회 Vol.2011 No.4

Effect of Extract from Laquer Tree (Rhus verniciflua Stokes) on DNCB-induced Atopic Dermatitis in NC/Nga Mouse

양정은,이정민,신헌섭,박상용,이돈길,Gafurjon T. Mavlonov,이우철,김소미,이태후 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.5

Laquer tree (Rhus verniciflua Stokes;Anacardiaceae, RVS) has been used in oriental medicines. This study examined whether the extract of RVS free of urushiol (detoxified RVS extract, DRE) exerting antiinflammation could alleviate the symptoms of atopic dermatitis (AD) induced with 2,4-dinitrochlorobenzene (DNCB) in NC/Nga mice. DRE contained flavonoids like fustin (153 mg/g), fisetin (14.6 mg/g), sulfuretin (13.3 mg/g), and quercetin (0.95 mg/g). DRE (10 μg/mL) decreased the production of nitric oxide activated by lipopolysaccharide. Oral administration of DRE (200 mg/kg weight/day)decreased scratching frequencies, the epidermal thickness on dorsal skin, and the number of degranulated mast cell. DRE significantly lowered the levels of IgE in sera (p<0.01),which were elevated by DNCB. DNCB reciprocally raised IL-4 and lowered IFN-γ in media of spleenocyte. DRE reversely raised IFN-γ and lowered IL-4. Contrastively,dexamethasone suppressed the overall immune reactions. In conclusion, DRE inhibited Th2-mediated IgE overproduction through modulation of Th1/2 balance, which was in part attributed to anti-inflammatory activity.

<i>Chryseobacterium gwangjuense</i> sp. nov., isolated from soil

Park, Yong Jin,Son, Heung-Min,Lee, Eun-Hee,Kim, Ju Han,Mavlonov, Gafurjon T.,Choi, Ki Ju,Shin, Hun-Sub,Kook, MooChang,Yi, Tae-Hoo International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.12

<P>A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterial strain, THG-A18<SUP>T</SUP>, was isolated from soil of Gwangju province in South Korea. Strain THG-A18<SUP>T</SUP> grew optimally at 25–30 °C, at pH 7.0–8.0 and in the absence of NaCl. Strain THG-A18<SUP>T</SUP> displayed β-glucosidase activity, which enabled it to convert ginsenoside Rb<SUB>1</SUB> to Rd. According to 16S rRNA gene sequence analysis, strain THG-A18<SUP>T</SUP> was shown to belong to the genus <I>Chryseobacterium</I>. The closest phylogenetic neighbours were <I>Chryseobacterium ginsenosidimutans</I> THG 15<SUP>T</SUP> (97.9 % 16S rRNA gene sequence similariity), <I>C. defluvii</I> B2<SUP>T</SUP> (97.7 %), <I>C. daeguense</I> K105<SUP>T</SUP> (97.6 %), <I>C. taiwanense</I> BCRC 17412<SUP>T</SUP> (97.5 %), <I>C. indoltheticum</I> LMG 4025<SUP>T</SUP> (97.4 %), <I>C. gregarium</I> P 461/12<SUP>T</SUP> (97.4 %) and <I>C. lathyri</I> RBA2-6<SUP>T</SUP> (97.3 %), but DNA–DNA relatedness values between these strains and strain THG-A18<SUP>T</SUP> were below 41.9 %. The G+C content of the genomic DNA was 36.4 mol%. The major respiratory quinone (MK-6) and fatty acids [iso-C<SUB>15 : 0</SUB>, iso-C<SUB>17 : 0</SUB> 3-OH, summed feature 3 (comprising C<SUB>16 : 1</SUB>ω7<I>c</I> and/or C<SUB>16 : 1</SUB>ω6<I>c</I>) and summed feature 9 (comprising iso-C<SUB>17 : 1</SUB>ω9<I>c</I> and/or 10-methyl C<SUB>16 : 0</SUB>)] supported the affiliation of strain THG-A18<SUP>T</SUP> with the genus <I>Chryseobacterium</I>. The polar lipids of strain THG-A18<SUP>T</SUP> were phosphatidylethanolamine, four unidentified aminolipids and seven unidentified lipids. A number of physiological and biochemical tests allowed phenotypic differentiation of strain THG-A18<SUP>T</SUP> from recognized species of the genus <I>Chryseobacterium</I>. The name <I>Chryseobacterium gwangjuense</I> sp. nov. is proposed, with THG-A18<SUP>T</SUP> ( = KACC 16227<SUP>T</SUP> = LMG 26579<SUP>T</SUP>) as the type strain.</P>

Ginsenoside F2 reduces hair loss by controlling apoptosis through the sterol regulatory element-binding protein cleavage activating protein and transforming growth factor-β pathways in a dihydrotestosterone-induced mouse model.

Shin, Heon-Sub,Park, Sang-Yong,Hwang, Eun-Son,Lee, Don-Gil,Mavlonov, Gafurjon Turdalievich,Yi, Tae-Hoo Pharmaceutical Society of Japan 2014 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.37 No.5

<P>This study was conducted to test whether ginsenoside F2 can reduce hair loss by influencing sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and the transforming growth factor beta (TGF-β) pathway of apoptosis in dihydrotestosterone (DHT)-treated hair cells and in a DHT-induced hair loss model in mice. Results for ginsenoside F2 were compared with finasteride. DHT inhibits proliferation of hair cells and induces androgenetic alopecia and was shown to activate an apoptosis signal pathway both in vitro and in vivo. The cell-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the proliferation rates of DHT-treated human hair dermal papilla cells (HHDPCs) and HaCaTs increased by 48% in the ginsenoside F2-treated group and by 12% in the finasteride-treated group. Western blot analysis showed that ginsenoside F2 decreased expression of TGF-β2 related factors involved in hair loss. The present study suggested a hair loss related pathway by changing SCAP related apoptosis pathway, which has been known to control cholesterol metabolism. SCAP, sterol regulatory element-binding protein (SREBP) and caspase-12 expression in the ginsenoside F2-treated group were decreased compared to the DHT and finasteride-treated group. C57BL/6 mice were also prepared by injection with DHT and then treated with ginsenoside F2 or finasteride. Hair growth rate, density, thickness measurements and tissue histotological analysis in these groups suggested that ginsenoside F2 suppressed hair cell apoptosis and premature entry to catagen more effectively than finasteride. Our results indicated that ginsenoside F2 decreased the expression of TGF-β2 and SCAP proteins, which have been suggested to be involved in apoptosis and entry into catagen. This study provides evidence those factors in the SCAP pathway could be targets for hair loss prevention drugs.</P>

Triangle Method for Fast Face Detection on the Wild

Malikovich, Karimov Madjit,Akhmatovich, Tashev Komil,ugli, Islomov Shahboz Zokir,Nizomovich, Mavlonov Obid Korea Multimedia Society 2018 The journal of multimedia information system Vol.5 No.1

There are a lot of problems in the face detection area. One of them is detecting faces by facial features and reducing number of the false negatives and positions. This paper is directed to solve this problem by the proposed triangle method. Also, this paper explans cascades, Haar-like features, AdaBoost, HOG. We propose a scheme using 12-net, 24-net, 48-net to scan images and improve efficiency. Using triangle method for frontal pose, B and B1 methods for other poses in neural networks are proposed.

<i>Arthrobacter bambusae</i> sp. nov., isolated from soil of a bamboo grove

Park, YongJin,Kook, MooChang,Ngo, Hien T. T.,Kim, Ki-Young,Park, Sang-Yong,Mavlonov, Gafurjon T.,Yi, Tae-Hoo International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.9

<P>A Gram-stain-positive, aerobic, motile by gliding, rod-shaped bacterial strain, THG-GM18<SUP>T</SUP>, was isolated from soil of a bamboo grove. Strain THG-GM18<SUP>T</SUP> was able to grow in the presence of up to 6.0 % (w/v) NaCl, at 4–37 °C and at pH 7.0–10.0 in R2A medium. Based on 16S rRNA gene sequence similarity, strain THG-GM18<SUP>T</SUP> was closely related to species of the genus <I>Arthrobacter</I>. The most closely related strains to strain THG-GM18<SUP>T</SUP> are <I>Arthrobacter ramosus</I> CCM 1646<SUP>T</SUP> (98.5 % similarity), <I>Arthrobacter nitroguajacolicus</I> G2-1<SUP>T</SUP> (98.4 %), <I>Arthrobacter nicotinovorans</I> DSM 420<SUP>T</SUP> (98.2 %), <I>Arthrobacter aurescens</I> DSM 20116<SUP>T</SUP> (98.1 %) and <I>Arthrobacter chlorophenolicus</I> A6<SUP>T</SUP> (98.0 %). Strain THG-GM18<SUP>T</SUP> possessed chemotaxonomic properties consistent with those of members of the genus <I>Arthrobacter</I>, such as peptidoglycan type A3α (<SMALL>l</SMALL>-Lys–<SMALL>l</SMALL>-Ala–<SMALL>l</SMALL>-Thr–<SMALL>l</SMALL>-Ala), MK-9 as major menaquinone and anteiso- and iso-branched compounds (anteiso-C<SUB>15 : 0</SUB>, iso-C<SUB>16 : 0</SUB> and anteiso-C<SUB>17 : 0</SUB>) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, unidentified phospholipids, unidentified aminolipids, an unidentified glycolipid and unidentified lipids. The G+C content of the genomic DNA was 61.0 mol%. The DNA–DNA relatedness values between strain THG-GM18<SUP>T</SUP> and its closest phylogenetic neighbours were below 26.0 %. The results of physiological and biochemical tests allowed the differentiation of strain THG-GM18<SUP>T</SUP> from species of the genus <I>Arthrobacter</I> with validly published names. <I>Arthrobacter bambusae</I> sp. nov. is the proposed name, and the type strain is THG-GM18<SUP>T</SUP> ( = KACC 17531<SUP>T</SUP> = JCM 19335<SUP>T</SUP>).</P>

Fucoidan Prevents the Progression of Osteoarthritis in Rats

Lee, Don-Gil,Park, Sang-Yong,Chung, Won-Seok,Park, Jae-Hee,Hwang, Eunson,Mavlonov, Gafurjon Tom,Kim, In-Ho,Kim, Ki-Young,Yi, Tae-Hoo Mary Ann Liebert 2015 Journal of medicinal food Vol.18 No.9

The inductive effect of ginsenoside F2 on hair growth by altering the WNT signal pathway in telogen mouse skin

Shin, H. S.,Park, S. Y.,Hwang, E. S.,Lee, D. G.,Song, H. G.,Mavlonov, G. T.,Yi, T. H. Elsevier Science B.V., Amsterdam. 2014 european journal of pharmacology Vol.730 No.-

This study was conducted to confirm the possibility of using minor ginseng saponin F2 by oral administration on hair anagen induction effects. The signaling pathway and anagen induction effect of ginsenoside F2 were investigated and compared with finasteride on the effect of hair growth induction. The cell-based MTT assay results indicated that the proliferation rates of HHDPC and HaCaT treated with F2 significantly increased by 30% compared with the finasteride-treated group. A western blot study showed that the expression of beta-catenin Lef-1 and DKK-1 increased by 140, 200% and decreased by 40% in the F2 treated group, respectively compared to that of finasteride-treated group. C57BL/6 mice were subjected to the same treatments. The hair growth promotion rates were compared with groups treated with finasteride, which was 20% higher in the F2 treated group. Tissue histological analysis results showed the number of hair follicles, thickness of the epidermis, and follicles of the anagen phase which increased in the F2 treated group, compared with the finasteride-treated groups. Moreover, the effect of F2 on hair growth was confirmed through the immunofluorescence (IF) methods indicating the expression aspect of Wnt signal pathway related factors in the tissue of C57BL/6 mouse. Our results considered the expression increase in beta-calenin, Lef-1 which was suggested as a major factor related to the development and growth of hair follicle and the decrease in DKK-1 when entering catagen by F2. As the data showed. F2 might be a potential new therapeutic source for anagen induction and hair growth through the Wnt signal pathway. (C) 2014 Elsevier B.V. All rights reserved.