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RISS 인기검색어
- 검색키워드
- 저자 : Gafurjon T. Mavlonov (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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<i>Arthrobacter bambusae</i> sp. nov., isolated from soil of a bamboo grove
Park, YongJin,Kook, MooChang,Ngo, Hien T. T.,Kim, Ki-Young,Park, Sang-Yong,Mavlonov, Gafurjon T.,Yi, Tae-Hoo International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.9
<P>A Gram-stain-positive, aerobic, motile by gliding, rod-shaped bacterial strain, THG-GM18<SUP>T</SUP>, was isolated from soil of a bamboo grove. Strain THG-GM18<SUP>T</SUP> was able to grow in the presence of up to 6.0 % (w/v) NaCl, at 4–37 °C and at pH 7.0–10.0 in R2A medium. Based on 16S rRNA gene sequence similarity, strain THG-GM18<SUP>T</SUP> was closely related to species of the genus <I>Arthrobacter</I>. The most closely related strains to strain THG-GM18<SUP>T</SUP> are <I>Arthrobacter ramosus</I> CCM 1646<SUP>T</SUP> (98.5 % similarity), <I>Arthrobacter nitroguajacolicus</I> G2-1<SUP>T</SUP> (98.4 %), <I>Arthrobacter nicotinovorans</I> DSM 420<SUP>T</SUP> (98.2 %), <I>Arthrobacter aurescens</I> DSM 20116<SUP>T</SUP> (98.1 %) and <I>Arthrobacter chlorophenolicus</I> A6<SUP>T</SUP> (98.0 %). Strain THG-GM18<SUP>T</SUP> possessed chemotaxonomic properties consistent with those of members of the genus <I>Arthrobacter</I>, such as peptidoglycan type A3α (<SMALL>l</SMALL>-Lys–<SMALL>l</SMALL>-Ala–<SMALL>l</SMALL>-Thr–<SMALL>l</SMALL>-Ala), MK-9 as major menaquinone and anteiso- and iso-branched compounds (anteiso-C<SUB>15 : 0</SUB>, iso-C<SUB>16 : 0</SUB> and anteiso-C<SUB>17 : 0</SUB>) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, unidentified phospholipids, unidentified aminolipids, an unidentified glycolipid and unidentified lipids. The G+C content of the genomic DNA was 61.0 mol%. The DNA–DNA relatedness values between strain THG-GM18<SUP>T</SUP> and its closest phylogenetic neighbours were below 26.0 %. The results of physiological and biochemical tests allowed the differentiation of strain THG-GM18<SUP>T</SUP> from species of the genus <I>Arthrobacter</I> with validly published names. <I>Arthrobacter bambusae</I> sp. nov. is the proposed name, and the type strain is THG-GM18<SUP>T</SUP> ( = KACC 17531<SUP>T</SUP> = JCM 19335<SUP>T</SUP>).</P>
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<i>Chryseobacterium gwangjuense</i> sp. nov., isolated from soil
Park, Yong Jin,Son, Heung-Min,Lee, Eun-Hee,Kim, Ju Han,Mavlonov, Gafurjon T.,Choi, Ki Ju,Shin, Hun-Sub,Kook, MooChang,Yi, Tae-Hoo International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.12
<P>A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterial strain, THG-A18<SUP>T</SUP>, was isolated from soil of Gwangju province in South Korea. Strain THG-A18<SUP>T</SUP> grew optimally at 25–30 °C, at pH 7.0–8.0 and in the absence of NaCl. Strain THG-A18<SUP>T</SUP> displayed β-glucosidase activity, which enabled it to convert ginsenoside Rb<SUB>1</SUB> to Rd. According to 16S rRNA gene sequence analysis, strain THG-A18<SUP>T</SUP> was shown to belong to the genus <I>Chryseobacterium</I>. The closest phylogenetic neighbours were <I>Chryseobacterium ginsenosidimutans</I> THG 15<SUP>T</SUP> (97.9 % 16S rRNA gene sequence similariity), <I>C. defluvii</I> B2<SUP>T</SUP> (97.7 %), <I>C. daeguense</I> K105<SUP>T</SUP> (97.6 %), <I>C. taiwanense</I> BCRC 17412<SUP>T</SUP> (97.5 %), <I>C. indoltheticum</I> LMG 4025<SUP>T</SUP> (97.4 %), <I>C. gregarium</I> P 461/12<SUP>T</SUP> (97.4 %) and <I>C. lathyri</I> RBA2-6<SUP>T</SUP> (97.3 %), but DNA–DNA relatedness values between these strains and strain THG-A18<SUP>T</SUP> were below 41.9 %. The G+C content of the genomic DNA was 36.4 mol%. The major respiratory quinone (MK-6) and fatty acids [iso-C<SUB>15 : 0</SUB>, iso-C<SUB>17 : 0</SUB> 3-OH, summed feature 3 (comprising C<SUB>16 : 1</SUB>ω7<I>c</I> and/or C<SUB>16 : 1</SUB>ω6<I>c</I>) and summed feature 9 (comprising iso-C<SUB>17 : 1</SUB>ω9<I>c</I> and/or 10-methyl C<SUB>16 : 0</SUB>)] supported the affiliation of strain THG-A18<SUP>T</SUP> with the genus <I>Chryseobacterium</I>. The polar lipids of strain THG-A18<SUP>T</SUP> were phosphatidylethanolamine, four unidentified aminolipids and seven unidentified lipids. A number of physiological and biochemical tests allowed phenotypic differentiation of strain THG-A18<SUP>T</SUP> from recognized species of the genus <I>Chryseobacterium</I>. The name <I>Chryseobacterium gwangjuense</I> sp. nov. is proposed, with THG-A18<SUP>T</SUP> ( = KACC 16227<SUP>T</SUP> = LMG 26579<SUP>T</SUP>) as the type strain.</P>
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양정은,이정민,신헌섭,박상용,이돈길,Gafurjon T. Mavlonov,이우철,김소미,이태후 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.5
Laquer tree (Rhus verniciflua Stokes;Anacardiaceae, RVS) has been used in oriental medicines. This study examined whether the extract of RVS free of urushiol (detoxified RVS extract, DRE) exerting antiinflammation could alleviate the symptoms of atopic dermatitis (AD) induced with 2,4-dinitrochlorobenzene (DNCB) in NC/Nga mice. DRE contained flavonoids like fustin (153 mg/g), fisetin (14.6 mg/g), sulfuretin (13.3 mg/g), and quercetin (0.95 mg/g). DRE (10 μg/mL) decreased the production of nitric oxide activated by lipopolysaccharide. Oral administration of DRE (200 mg/kg weight/day)decreased scratching frequencies, the epidermal thickness on dorsal skin, and the number of degranulated mast cell. DRE significantly lowered the levels of IgE in sera (p<0.01),which were elevated by DNCB. DNCB reciprocally raised IL-4 and lowered IFN-γ in media of spleenocyte. DRE reversely raised IFN-γ and lowered IL-4. Contrastively,dexamethasone suppressed the overall immune reactions. In conclusion, DRE inhibited Th2-mediated IgE overproduction through modulation of Th1/2 balance, which was in part attributed to anti-inflammatory activity.
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