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- 저자 : Matsumoto Yoshiyuki (검색결과 5 건)
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Expression of Hyaluronidase-4 in a Rat Spinal Cord Hemisection Model
Yoshiyuki Tachi,Tetsuhito Okuda,Norio Kawahara,Nobuo Kato,Yasuhito Ishigaki,Tadami Matsumoto 대한척추외과학회 2015 Asian Spine Journal Vol.9 No.1
Study Design: Examination of hyaluronidase-4 (Hyal-4) expression in a rat spinal cord hemisection model. Purpose: To determine the status of Hyal-4 expression after hemisection of the spinal cord, and the relationship between its expression and that of chondroitin sulfate proteoglycans (CSPGs). Overview of Literature: CSPGs are expressed at the site of spinal cord injury and inhibit axon regeneration. Administration of exogenous chrondroitinase ABC (ChABC), derived from bacteria, digested CSPGs and promoted axonal regrowth. Using a rat hemisection model, we have demonstrated peak CSPGs levels at by 3 weeks after injury but then decreased spontaneously. Could there be an endogenous enzyme similar to ChABC in the spinal cord? It has been suggested that Hyal-4 is involved in CSPG degradation. Methods: A rat hemisection model was prepared and spinal cord frozen sections were prepared at 4 days and 1, 2, 3, 4, 5, and 6 weeks post-cordotomy and stained for CSPGs and Hyal-4 and subjected to Western blotting. Results: CSPGs appeared at the injury site at 4 days after hemisection, reached a peak after 3 weeks, and then decreased. Hyal-4 was observed around the injury site from 4 days after cordotomy and increased until after 5–6 weeks. Double staining showed Hyal-4 around CSPGs. Western blotting identified a band corresponding to Hyal-4 from 4 days after hemisection. Conclusions: Hyal-4 was expressed in a rat hemisection model in areas surrounding CSPGs, and as its peak was delayed compared with that of CSPGs. These results suggest the involvement of Hyal-4 in the digestion of CSPGs.
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Takehisa Matsumoto,Masatomo Kawakubo,Mayumi Shiohara,Toshiko Kumagai,Eiko Hidaka,Kazuyoshi Yamauchi,Kozue Oana,Kenji Matsuzawa,Hiroyoshi Ota,Yoshiyuki Kawakami 한국미생물학회 2009 The journal of microbiology Vol.47 No.2
“Helicobacter heilmannii” is an uncultivable spiral-shaped bacterium inhabiting the human gastric mucosa. It is larger and more tightly-coiled than H. pylori. We encountered a patient with chronic gastritis infected a “H. heilmannii”-like organism (HHLO), designated as SH6. Gastric mucosa derived from the patient was orally ingested by specific pathogen free mice. Colonization of the mice by SH6 was confirmed by electron microscopy of gastric tissue specimens. In an attempt to characterize SH6, 16S rRNA and urease genes were sequenced. The 16S rRNA gene sequence was most similar (99.4%; 1,437/1,445 bp) to HHLO C4E from a cheetah. However, the urease gene sequence displayed low similarity (81.7%; 1,240/1,516 bp) with HHLO C4E. Taxonomic analysis disclosed that SH6 represents a novel strain and should constitute a novel taxon in the phylogenetic trees, being discriminated from any other taxon, with the ability of infecting human gastric mucosa.
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Matsuura Takumi,Yamamoto Tetsuya,Matsumoto Yoshiyuki,Itino Takao 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4
We reconstructed Stomaphis phylogeny and analyzed evolutionary host-plant shifts. The molecular phylogeny revealed 23 well-supported lineages, each specialized to use specific host plant family, whilst host plants of Stomaphis aphids ranged across 6 orders, 11 families, 21 genera, and 28 species. This combination of high host specificity with evolutionarily distant host shifts is exceptional in herbivores. To explain this pattern, we propose one hypothesis among several possibilities: Stomaphis aphids are generalists with respect to the defensive chemicals produced by the plant, but specialists with respect to the stem surface structure of the host tree. This hypothesis predicts that tree taxa having stem surface structures preferred by Stomaphis would be used again and again by genetically distinct Stomaphis lineages. Consistent with this prediction, we found that different (occa sionally phylogenetically distant) Stomaphis lineages shared the same host plant genera such as Alnus and Betula. This result suggests that, in the course of their evolutionary history, Japanese Stomaphis aphids have repeatedly colonized a limited number of host plant genera.
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Furukawa Mitsuru,Okuyama Kunimasa,Ninomiya Ken,Yato Yoshiyuki,Miyamoto Takeshi,Nakamura Masaya,Matsumoto Morio 대한척추외과학회 2022 Asian Spine Journal Vol.16 No.1
Study Design: Cross-sectional study.Purpose: To examine whether the number of continuous vertebral bone bridges and bone mineral density (BMD) influence the fracture risk in diffuse idiopathic skeletal hyperostosis (DISH) patients.Overview of Literature: Bone bridges connecting through the intervertebral body in DISH create long lever arms that can increase the risk of fractures from minor trauma. DISH patients have a BMD that is higher than or comparable to those of age-matched healthy subjects.Methods: We examined the computed tomography scans from the thoracic vertebra to the sacrum used to diagnose DISH in 140 patients (98 men and 42 women; average age, 78.6 years). We compared patients who did (n=52) and did not have (n=88) fractures at the continuous vertebral bodies fused by bone bridges. The relationship between the vertebral fractures and the maximum number of vertebrae that are bony cross-linked with contiguous adjacent vertebrae (max VB) from the thoracic vertebra to the sacrum or from the lumbar vertebra to the sacrum and proximal femur BMD were analyzed using a logistic regression model.Results: We found that after adjusting for the confounding factors, higher max VB, both from the thoracic vertebrae to the sacrum and the lumbar vertebrae to the sacrum, was associated with a higher risk of vertebral fractures. This difference was statistically significant. The risk was higher when only the lumbar vertebrae to the sacrum was considered (thoracic vertebrae to the sacrum: odds ratio, 1.21; p<0.05; lumbar vertebrae to the sacrum: odds ratio, 2.78; p<0.01). Moreover, low proximal femur BMD in DISH patients raises the fracture risk (odds ratio, 0.47; p<0.01).Conclusions: Many continuous vertebral bone bridges, especially those that extend to the lumbar spine and low proximal femur BMD, are risk factors for fracture in DISH patients.
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Yuanxia Sun,Yupin Guan,Shigeru Hayakawa,Masahiro Ogawa,Supaporn Naknukool,Yoshiyuki Matsumoto 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.2
Solid wastes of freshwater clam in food processing, including mainly mantle, were used as a raw material for the recovery of bioactive peptides related to angiotensin I-converting enzyme (ACE) inhibitor. Among the primary hydrolysates of dried mantle (DM), the peptides corresponding to hydrolysates using 2 crude peptidases exhibited a strong ACE inhibitory activity (IC_50, 0.23 mg/mL), and recovery efficiency of soluble materials and their protein content were considerably large with 42.65% and 468.6 mg/g, respectively. The ACE inhibitory activity of all secondary hydrolysates digested by pepsin and trypsin was significantly increased as compared to primary hydrolysates. Furthermore, the peptic secondary hydrolysates were fractionated by gel filtration and reverse phase-HPLC (RP-HPLC) and characterized by matrix-assisted laser desorption-ionization time of flight/mass spectrometry (MALDI-TOF/MS). These peptides with molecular weight of less than 1 kDa possessed the stronger ACE inhibitory effect, and their inhibitory pattern was found to be competitive. The results showed that the DM hydoplysates might be utilized as a rich source of bioactive peptide.
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