Pollen/Fruit Syndrome: Clinical Relevance of the Cypress Pollen Allergenic Gibberellin-Regulated Protein

Hélène Sénéchal,Sanaz Keykhosravi,Rémy Couderc,Marie-Ange Selva,Youcef Shahali,Tomoyasu Aizawa,Jean-Marc Busnel,Rihane Arif,Inna Mercier,Nhan Pham-Thi,Denis-André Charpin,Pascal Poncet 대한천식알레르기학회 2019 Allergy, Asthma & Immunology Research Vol.11 No.1

A pollen/food-associated syndrome (PFAS) has been described between peach and cypress pollen. Cross-reactive allergens were characterized which belong to the Gibberellin-regulated protein (GRP) family, BP14 in cypress pollen and Pru p 7 in peach. GRP are small cationic protein with anti-microbial properties. A patient suffering from a peach/cypress syndrome was explored clinically and biologically using 2 types of immunoglobulin E (IgE) multiarray microchip, immunoblots and a basophil activation test to assess the clinical relevance of various extracts and purified allergens from fruits or cypress pollen. In addition to PR10 sensitization, the patient showed specific IgE to Pru p 7, BP14 and allergen from pomegranate. These last 3 allergens and allergenic sources are able to induce ex vivo basophil activation characterized by the monitoring of the expression of CD63 and CD203c, both cell surface markers correlated with a basophil mediator release. Up to 100% of cells expressed CD203c at 50 ng/mL of BP14 protein. In contrast, snakin-1, a GRP from potato sharing 82% sequence identity with Pru p 7 did not activate patient's basophils. These results strongly suggest that, like Pru p 7, BP14 is a clinically relevant allergenic GRP from pollen. Allergen members of this newly described protein family are good candidates for PFAS where no cross-reactive allergens have been characterized.

Genes Involved in Interleukin-1 Receptor Type II Activities Are Associated With Asthmatic Phenotypes

Anne-Marie Madore,Vanessa T. Vaillancourt,Emmanuelle Bouzigon,Chloé Sarnowski,Florent Monier,Marie-Hélène Dizier,Florence Demenais,Catherine Laprise 대한천식알레르기학회 2016 Allergy, Asthma & Immunology Research Vol.8 No.5

Purpose: Interleukin-1 (IL-1) plays a key role in inflammation and immunity and its decoy receptor, IL-1R2, has been implicated in transcriptomic and genetic studies of asthma. Methods: Two large asthma family collections, the French-Canadian Saguenay—Lac-St-Jean (SLSJ) study and the French Epidemiological Study on the Genetics and Environment of Asthma (EGEA), were used to investigate the association of SNPs in 10 genes that modulate IL-1R2 activities with asthma, allergic asthma, and atopy. Gene-gene interactions were also tested. Results: One SNP in BACE2 was associated with allergic asthma in the SLSJ study and replicated in the EGEA study before statistical correction for multiple testing. Additionally, two SNPs in the MMP2 gene were replicated in both studies prior to statistical correction and reached significance in the combined analysis. Moreover, three gene-gene interactions also survived statistical correction in the combined analyses (BACE1-IL1RAP in asthma and allergic asthma and IL1R1-IL1RAP in atopy). Conclusions: Our results highlight the relevance of genes involved in the IL-1R2 activity in the context of asthma and asthma-related traits.

Capsicum Allergy: Involvement of Cap a 7, a New Clinically Relevant Gibberellin-Regulated Protein Cross-Reactive With Cry j 7, the Gibberellin-Regulated Protein From Japanese Cedar Pollen

Takei Mari,Nin Charles,Iizuka Tomona,Pawlikowski Marine,Selva Marie-Ange,Chantran Yannick,Nakajima Yurie,Zheng Jingkang,Aizawa Tomoyasu,Ebisawa Motohiro,Sénéchal Hélène,Poncet Pascal 대한천식알레르기학회 2022 Allergy, Asthma & Immunology Research Vol.14 No.3

The Capsicum genus belongs to the Solanaceae family. Bell or chili peppers are consumed worldwide, but allergy to Capsicum is rare. It is involved in the celery-birch-mugwort-spice syndrome and cross-reactivities were reported with latex. Several allergens have been described, but only 2 are referenced in the World Health Organization/International Union of Immunological Societies allergen data bank, a thaumatin-like protein and a profilin. A patient allergic to bell/chili pepper, peach, orange and Japanese cedar pollen was clinically and biologically analyzed including direct and competitive immunoblots and basophil activation tests (BATs) with allergenic source extracts and recombinant gibberellin-regulated proteins (GRPs). The patient was shown to be sensitized to Cap a 7, the GRP of Capsicum annuum newly described herein. Cross-reactivities were demonstrated between various GRPs from bell/chili pepper, peach, orange and Japanese cedar pollen either in native form in the different extracts or as recombinant allergens. A similar immunoglobulin E reactivity was found also in Capsicum chinense and against snakin-1, the GRP from potato. The patient showed a positive BAT with recombinant Cry j 7, Pru p 7 and Cap a 7, but not with recombinant snakin-1. Despite the ubiquitous nature of GRPs in plants and the immunochemical cross-reactivity observed between different GRPs, clinically relevant sensitization to this protein family seems restricted to some allergenic sources, often associated with Cupressaceae pollen allergy, and to some patients, therefore reflecting very specific and peculiar mechanisms of conditional sensitization.

SYNTHESIS AND MICROSTRUCTURE OF A NOVEL TiO2 AEROGEL–TiO2

YOSHIKAZU SUZUKI,MARIE-HÉLÈNE BERGER,DANIELA D'ELIA,PIERRE ILBIZIAN,CHRISTIAN BEAUGER,ARNAUD RIGACCI,JEAN-FRANÇOIS HOCHEPIED,PATRICK ACHARD 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2008 NANO Vol.3 No.5

TiO2 aerogel–10 mol% TiO2 nanowire composite was prepared by a sol–gel technique with the addition of TiO2 nanowires to TiO2 sol, followed by supercritical drying in CO2. TiO2 nanowires (anatase with minor rutile phases) as dispersoid were prepared by a hydrothermal process followed by calcination in air at 600°C. The TiO2 nanowires were dispersed in a 2-propanol/H2O/HNO3 solution, and the mixture was added drop by drop to a tetrabutyl orthotitanate [i.e. Ti (IV) n-butoxide] solution in 2-propanol. After gelation, the TiO2 alcogel–TiO2 nanowire composite was dried in supercritical CO2 to obtain the final, TiO2 aerogel–TiO2 nanowire composite. TEM analysis revealed that a unique "nanowire network" structure was formed withinthe mesoporous aerogel matrix. The aerogel–TiO2 nanowire composite had a relatively large surface area 427 m2/g, with mesopores ~ 16 nm in diameter and a pore of volume of 1.63 cm3/g.

Monitoring of 45 Pesticides in Lebanese Surface Water using Polar Organic Chemical Integrative Sampler (POCIS)

Al Ashi Aisha,Wael Hneine,Samia Mokh,Marie-Hélène Devier,Hélèn Budzinski,Farouk Jaber 한국해양과학기술원 2017 Ocean science journal Vol.52 No.3

The aim of this study is to assess the dissolved concentration of 45 pesticides in the surface waters of the Lebanese Republic using Polar Organic Chemical Integrative Sampler "POCIS". All of the sampling sites are located in the major agricultural land areas in Lebanon. POCIS (n = 3) were deployed at Ibrahim River, Qaraoun Lake and Hasbani River for a duration of 14 days. The total concentration of pesticides ranged from not detected (nd) to 137.66 ng.L-1. Chlorpyrifos, DDE-pp, diazinon and Fenpropathrin were the most abundant compounds. Qaraoun Lake and Hasbani River were found to be more polluted than Ibrahim River, since they receive large amounts of waste water derived from nearby agricultural lands and they had the lowest dilution factor. The aqueous average concentration of the target compounds were estimated using sampling rates obtained from the literature. Comparison between Time Weighed Average concentrations "TWA" using POCIS and spot sampling is presented. Results showed that POCIS TWA concentrations are in agreement with spot sampling concentrations for Ibrahim and Hasbani Rivers. The toxicity of the major detected pesticides on three representative aquatic species (Daphnia magna, Scenedesmus quadricauda and Oncorhynchus mykiss) is also reported.

Accuracy of a separating foil impression using a novel polyolefin foil compared to a custom tray and a stock tray technique

Marie-Hélène Pastoret,Gabriel Krastl,Julia Bühler,Roland Weiger,Nicola Ursula Zitzmann 대한치과보철학회 2017 The Journal of Advanced Prosthodontics Vol.9 No.4

PURPOSE. To compare the dimensional accuracy of three impression techniques- a separating foil impression, a custom tray impression, and a stock tray impression. MATERIALS AND METHODS. A machined mandibular complete-arch metal model with special modifications served as a master cast. Three different impression techniques (n = 6 in each group) were performed with addition-cured silicon materials: i) putty-wash technique with a prefabricated metal tray (MET) using putty and regular body, ii) single-phase impression with custom tray (CUS) using regular body material, and iii) two-stage technique with stock metal tray (SEP) using putty with a separating foil and regular body material. All impressions were poured with epoxy resin. Six different distances (four intra-abutment and two inter-abutment distances) were gauged on the metal master model and on the casts with a microscope in combination with calibrated measuring software. The differences of the evaluated distances between the reference and the three test groups were calculated and expressed as mean (± SD). Additionally, the 95% confidence intervals were calculated and significant differences between the experimental groups were assumed when confidence intervals did not overlap. RESULTS. Dimensional changes compared to reference values varied between -74.01 and 32.57 탆 (MET), -78.86 and 30.84 (CUS), and between -92.20 and 30.98 (SEP). For the intra-abutment distances, no significant differences among the experimental groups were detected. CUS showed a significantly higher dimensional accuracy for the inter-abutment distances with -0.02 and –0.08 percentage deviation compared to MET and SEP. CONCLUSION. The separation foil technique is a simple alternative to the custom tray technique for single tooth restorations, while limitations may exist for extended restorations with multiple abutment teeth

Stabilization of monodisperse electrosprays in the multi-jet mode via electric field enhancement

Duby, Marie-Hé,lè,ne,Deng, Weiwei,Kim, Kyoungtae,Gomez, Tommaso,Gomez, Alessandro Elsevier 2006 Journal of aerosol science Vol.37 No.3

<P><B>Abstract</B></P><P>The electrospray of conducting liquids operated in the cone-jet mode is well known to have the unique ability of generating droplets uniform in size over a phenomenal range of sizes depending primarily on the liquid flow rate and physical properties. Since there is a monotonic dependence of size on flow rate, the liquid flow rates that can be dispersed are modest if the goal is to produce very small (below a few micrometers in diameter) droplets. Yet, this is precisely the application niche for which few, if any, atomization alternatives are available. Multiplexing the spray source is indispensable for the electrospray capabilities to have an impact in high-value-added applications. We report here on a novel approach to multiplexing based on a well-known, but hitherto unexploited, regime of operation, the multi-jet mode. Ordinarily, such a mode is rather unsteady and the range of flow rates at which appreciable multiplexing is achieved is small. However, if the multi-jet mode is anchored by some sharp features (e.g., grooves, ridges, etc.) machined at the outlet of the atomizer, to intensify the electric field at discrete points around its perimeter, then the cone-jets are simultaneously anchored at these features and a stable mode of operation is identified over several hundreds of volts and a broad range of flow rates. Most importantly, so long as the machining is accurately reproduced from point to point, droplets generated do not vary significantly in size from spray to spray. As a result, a compact, inexpensive and versatile multiplexing system is realized without sacrificing droplet monodispersity.</P>