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( Maria Adela Aguirre ),( Maria Lourdes Posadas Martinez ),( Melisa Blomberg ),( Dorotea Beatriz Fantl ),( Diego Hernan Giunta ),( Fernan Gonzalez Bernaldo De Quiroz ),( Maria Florencia Grande Ratti ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Cardiac involvement is the leading cause of morbidity and mortality of amy-loidosis, especially due to light-chain (AL) and transthyretin, both wild and hereditary types. Objectives: To estimate the prevalence and mortality of patients with evidence of cardiac involvement in patients diagnosed with amyloidosis. Methods: Ambispective cohort study, with data obtained from all patients included between 01/2007 and 03/2013 with evidence of amyloidosis in the Institutional Regis-try Amyloidosis of the Hospital Italiano de Buenos Aires. The diagnostic criteria of cardiac amyloidosis were predefined. Mortality from cardiac amyloidosis was measured using a combination of active and passive assessment. Survival time was evaluated using the Kaplan-Meier estimator. SPSS 19.0 was used. Results: The registry included 126 patients and the prevalence of cardiac amyloidosis was 40% (95%, CI:31-49%). Among the 40% (50/126) of patients with confirmed amyloidosis and suspected of cardiac involvement, the median age was 72 years (IQR 79-81) and 26% were women (13/50). Suspected diagnosis of cardiac involvement was: clinical 82.4% (42/50), from complementary studies 67% (34/50), and diagnosed from another hospital 6% (3/50). From all patients with suspected cardiac involvement, 47% had con- firmed biopsy. Overall mortality of patients with cardiac involvement was 20% (11/50), the median survival was 1782 days of follow up (CI 95% 1365-2199). Conclusions: The prevalence of cardiac involvement and mortality in patients with amyloidosis was high. Advances in diagnosis of the possible causes of amyloidosis in the future will allow the detection of patients with high risk of death from cardiac causes in our country.
Cystic Fibrosis: Clinical Phenotypes in Children and Adolescents
dos Santos, Ana Luiza Melo,de Melo Santos, Helen,Nogueira, Marina Bettiol,Tavora, Hugo Tadashi Oshiro,da Cunha, Maria de Lourdes Jaborandy Paim,de Melo Seixas, Renata Belem Pessoa,Monte, Luciana de Fr The Korean Society of Pediatric Gastroenterology 2018 Pediatric gastroenterology, hepatology & nutrition Vol.21 No.4
Purpose: The objective of this study was to describe the clinical phenotypes of children and adolescents with cystic fibrosis (CF); and to assess the role of pancreatic insufficiency and neonatal screening in diagnosis. Methods: A cross-sectional study was conducted, which included 77 patients attending a reference center of CF between 2014 and 2016. Epidemiological data, anthropometric measurements, and the presence of pulmonary, pancreatic, gastrointestinal and hepatobiliary manifestations were evaluated based on clinical data and complementary examinations. Results: Of the 77 patients, 51.9% were male, with a median age of 147 months (7.0-297.0 months), and the majority showed adequate nutritional status. The most common phenotype was pulmonary (92.2%), followed by pancreatic (87.0%), with pancreatic insufficiency in most cases. Gastrointestinal manifestation occurred in 46.8%, with constipation being the more common factor. Hepatobiliary disease occurred in 62.3% of patients. The group with pancreatic insufficiency was diagnosed earlier (5.0 months) when compared to the group with sufficiency (84.0 months) (p=0.01). The age of diagnosis was reduced following implementation of neonatal screening protocols for CF (6.0 months before vs. 3.0 months after, p=0.02). Conclusion: The pulmonary phenotype was the most common, although extrapulmonary manifestations were frequent and clinically relevant, and should mandate early detection and treatment. Neonatal screening for CF led to earlier diagnosis in patients with pancreatic failure, and therefore, should be adopted universally.
Cystic Fibrosis: Clinical Phenotypes in Children and Adolescents
Ana Luiza Melo dos Santos,Helen de Melo Santos,Marina Bettiol Nogueira,Hugo Tadashi Oshiro Távora,Maria de Lourdes Jaborandy Paim da Cunha,Renata Belém Pessoa de Melo Seixas,Luciana de Freitas Velloso 대한소아소화기영양학회 2018 Pediatric gastroenterology, hepatology & nutrition Vol.21 No.4
Purpose: The objective of this study was to describe the clinical phenotypes of children and adolescents with cystic fibrosis (CF); and to assess the role of pancreatic insufficiency and neonatal screening in diagnosis.Methods: A cross-sectional study was conducted, which included 77 patients attending a reference center of CF between 2014 and 2016. Epidemiological data, anthropometric measurements, and the presence of pulmonary, pancreatic, gastrointestinal and hepatobiliary manifestations were evaluated based on clinical data and complementary examinations.Results: Of the 77 patients, 51.9% were male, with a median age of 147 months (7.0-297.0 months), and the majority showed adequate nutritional status. The most common phenotype was pulmonary (92.2%), followed by pancreatic (87.0%), with pancreatic insufficiency in most cases. Gastrointestinal manifestation occurred in 46.8%, with constipation being the more common factor. Hepatobiliary disease occurred in 62.3% of patients. The group with pancreatic insufficiency was diagnosed earlier (5.0 months) when compared to the group with sufficiency (84.0 months) (p=0.01). The age of diagnosis was reduced following implementation of neonatal screening protocols for CF (6.0 months before vs. 3.0 months after, p=0.02).Conclusion: The pulmonary phenotype was the most common, although extrapulmonary manifestations were frequent and clinically relevant, and should mandate early detection and treatment. Neonatal screening for CF led to earlier diagnosis in patients with pancreatic failure, and therefore, should be adopted universally.
( Maria Lourdes Posadas Martinez ),( Fernando Javier Vazquez ),( Fernan Gonzalez Bernaldo De Quiros ),( Liliana Paloma Rojas ),( Gabriel Dario Waisman ),( Diego Hernan Giunta ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Venous thromboembolism (VTE) is the most common cause of preventable mortality in hospitalized patients. On plus, pulmonary embolism (PE) is responsible for 5-10% of all hospital deaths. Objective: To estimate the incidence density (ID) and hospital mortality of VTE among clinical and surgical inpatients. Methods: Prospective cohort, during a period of 7 years, of incident cases of VTE, PE, and deep venous thrombosis (DVT), in patients of 17 years and older, hospitalized at the internal medicine and surgery department of a tertiary care hospital in Buenos Aires. Fatality rate of patients with VTE was calculated. Results: 2042 episodes of VTE were registered. Sixty two percent (1258) were patients from the Internal Medicine Service: 635 cases (95%, CI:601-671) presented VTE, 446 cases (95%, CI:418-476) presented DVT and 286 cases (95% CI:264-311) presented PE per 1000 person-days of hospitalization. For patients hospitalized at surgery department: 102 cases (95%, CI:96-110), 83 (95%, CI: 77-90) and 31 cases (95%, CI: 27-35) per 1000 person-days of hospitalization presented VTE, DVT and PE respectively. The VTE represented between 3 to 9 % of hospital deaths, it increases with age independently of being clinical or surgical inpatients. Conclusions: In Argentina, there are few data of incidences of VTD and hospital mortality in patients with VTE. This information may be important when assessing the need for resources for prevention, diagnosis and treatment in hospitals.
( Maximiliano Toscano ),( Maria Lourdes Posadas Martinez ),( Pablo Marcelo Ajler ),( Maria Victoria Franco ),( Maria Teresa Garcia Botta ),( Ezequiel Goldschmidt ),( Fernan Gonzalez Bernaldo De Quiros 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Venous thromboembolism (VTE) includes deep vein thrombosis (DVT) and pulmonary embolism (PE). VTE represents the fi rst cause of preventable morbidity and mortality in neurosurgery, these patients have many additional risk factors for VTE, but thromboprophylaxis is discussed for threatened complications. Estimated annual incidence in the United States is 600, 000 cases, with a mortality of nearly 17% at 3 months after diagnosis. Objetive: To estimate the incidence of postoperative VTE in patients with neurosurgery and to describe thrombophylactic measures, assigned treatment and associated complications. Methods: Retrospective cohort of all patients (=17 years) who underwent a neurosurgical procedure from January 2010 to January 2012. All patients were followed up to 90 days to evaluate the presence of VTE and/or death. Baseline characteristics, thromboprophylaxis, treatment for VTE and complications were evaluated. Results: During two years, 321 patients met the inclusion criteria. VTE incidence was 3. 1% (10 cases, 95% CI:1. 2%-4. 9%), 5 patients developed DVT, the other half developed both DVT and PE. 52% were female, median age was 55 (RIC 35-66) years. From the total of patients, 71% (229) received thromboprophylaxis, from which 58% implemented mechanical thromboprophylaxis and 42% received associated pharmacologic prophylaxis. 4 of the 10 patients with DVT were receiving prophylaxis at the time of the event. Not receiving thromboprophylaxis increased 1. 5 times (95%, CI:0. 95-2. 38, p=0. 001) the risk of presenting VTE that in those who received thromboprophylaxis. The risk of developing PE was 4 times (95%, CI:0. 73-22%, p=0. 001) bigger in those who didn´t receive thromboprophylaxis. 80% of patients received treatment for VTE: anticoagulants (4), thrombolytics and anticoagulants (1), thrombolytics (1), thrombolytics and fi lter (1) and 1 received fi lter and anticoagulation. For complications, no ETV patients died, one required hospitalization and 6. 9% (22) had major bleedings. Conclusions: VTE remains an important complication in neurosurgical patients.
( Cecilia Ines Ono Morikawa ),( Rie Miyaura ),( Maria De Lourdes Tapia Y Figueroa ),( Elsa Liliana Rengifo Salgado ),( Yoshiharu Fujii ) 한국잡초학회 2012 Weed Biology and Management Vol.12 No.1
Peru is one of the 20 botanically extremely diverse countries in the world, with >17 000 flowering plants, of which 30% are endemic. So far, no systematic research has been conducted on the screening of the allelopathic plants. In this study, the allelopathic activity of 170 species from 61 families of Peruvian plants that were collected from the three main regions of Peru - the Costa (Pacific coastline), the Sierra (Andean mountains), and the Selva (Amazonian rainforest) - was evaluated.The allelopathic activity was determined by the Sandwich Method, which can evaluate the activity of leaf leachates. The species that were found to be highly inhibitory in this screening, under the criterion of >90% inhibition of the radicle of lettuce (Lactuca sativa) seedlings, were Aristeguietia ballii and Diplostephium foliosissimum (Asteraceae) and Spondias mombin (Anacardiaceae). All of these species are native plants from Peru.This study gives a strong clue regarding the potential of isolating potent allelochemicals from these plants in the future.
Meza-Espinoza, Libier,Vivar-Vera, Maria de los Angeles,Garcia-Magana, Maria de Lourdes,Sayago-Ayerdi, Sonia G.,Chacon-Lopez, Alejandra,Becerrea-Verdin, Eduardo M.,Montalvo-Gonzalez, Efigenia 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
The enzymatic activity and partial characterization of proteases from Bromelia karatas fruits were evaluated and compared with Bromelia pinguin proteases. The specific activity increased twofold after partial purification in both proteases. Partially purified proteases from Bromelia karatas showed good specific activity at pH 6.0-8.0 and residual activity of 70-100% for 60 min at $37-60^{\circ}C$, similar to Bromelia pinguin proteases. The $K_m$ value of proteases from Bromelia karatas was higher ($253.32{\mu}M$) than that of Bromelia pinguin proteases ($234.94{\mu}M$). The use of specific protease inhibitors indicated the presence of cysteine and serine proteases. Proteases with molecular weight of 66.2-97 and 21-31 kDa were detected. Bromelia karatas proteases registered 73% hydrolysis using a soy protein concentrate, similar to the enzyme activity of Bromelia pinguin proteases and commercial bromelain. These results demonstrate that Bromelia karatas proteases could be a potential alternative protease in the food industry.
Biochemical Properties of an Extracellular Trehalase from Malbranchea pulchella var. Sulfurea
Marita Gimenez Pereira,Luis Henrique Souza Guimarães,Rosa Prazeres Melo Furriel,Maria de Lourdes,Hector Francisco Terenzi,Joao Atilio Jorge 한국미생물학회 2011 The journal of microbiology Vol.49 No.5
The thermophilic fungus Malbranchea pulchella var. sulfurea produced good amounts of extracellular trehalase activity when grown for long periods on starch, maltose or glucose as the main carbon source. Studies with young cultures suggested that the main role of the extracellular acid trehalase is utilizing trehalose as a carbon source. The specific activity of the purified enzyme in the presence of manganese (680 U/mg protein) was comparable to that of other thermophilic fungi enzymes, but many times higher than the values reported for trehalases from other microbial sources. The apparent molecular mass of the native enzyme was estimated to be 104 kDa by gel filtration and 52 kDa by SDS-PAGE, suggesting that the enzyme was composed by two subunits. The carbohydrate content of the purified enzyme was estimated to be 19% and the pI was 3.5. The optimum pH and temperature were 5.0-5.5 and 55°C, respectively. The purified enzyme was stimulated by manganese and inhibited by calcium ions, and insensitive to ATP and ADP, and 1 mM silver ions. The apparent K_M values for trehalose hydrolysis by the purified enzyme in the absence and presence of manganese chloride were 2.70±0.29 and 2.58±0.13 mM, respectively. Manganese ions affected only the apparent V_(max), increasing the catalytic efficiency value by 9.2-fold. The results reported herein indicate that Malbranchea pulchella produces a trehalase with mixed biochemical properties, different from the conventional acid and neutral enzymes and also from trehalases from other thermophilic fungi.
Paulo Gustavo Kotze,Daniela Oliveira Magro,Barbara Saab,Mansur Paulo Saab,Lilian Vital Pinheiro,Marcia Olandoski,Maria de Lourdes Setsuko Ayrizono,Carlos Augusto Real Martinez,Claudio Saddy Rodrigues 대한장연구학회 2018 Intestinal Research Vol.16 No.1
Background/Aims: The use of anti-tumor necrosis factor (anti-TNF) agents seems to reduce surgical rates and delay surgicalprocedures in prospective trials and population-based studies in the management of Crohn’s disease (CD). This study aimedto identify whether preoperative anti-TNF agents influence the time from diagnosis to surgery. Methods: An observationalretrospective cohort study was conducted on patients with CD submitted to intestinal resections due to complications ormedical therapy failure in a period of 7 years. The patients were allocated into 2 groups according to their previous exposure toanti-TNF agents in the preoperative period. Epidemiological aspects regarding age at diagnosis, smoking, perianal disease, andpreoperative conventional therapy were considered. A Kaplan-Meier survival analysis was used to outline possible differencesbetween the groups regarding the time to surgery. Results: A total of 123 patients were included (71 and 52 with and withoutprevious exposure to biologics, respectively). The overall time to surgery was 108±6.9 months (maximum, 276 months). The survival estimation revealed no difference in the mean time to intestinal resection between the groups (99.78±10.62 months inthe patients without and 114.01±9.07 months in those with previous anti-TNF use) (log-rank P =0.35). There was no significantdifference in the time to surgery regarding perianal CD (P =0.49), smoking (P =0.63), preoperative azathioprine (P =0.073) andsteroid use (P =0.58). Conclusions: The time from diagnosis to surgery was not influenced by the preoperative use of anti-TNFtherapy in this cohort of patients.
( Brandi Jamile ),( Eder C. Oliveira ),( Nilson K. Monteiro ),( Ana Flora D. Vasconcelos ),( Robert F. H. Dekker ),( Aneli M. Barbosa ),( Joana L. M. Silveira ),( Paulo A. S. Mourao ),( Maria De Lourd 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.10
The exopolysaccharide botryosphaeran (EPS(GLC); a (1→ 3)(1→6)-β-D-glucan from Botryosphaeria rhodina MAMB- 05) was sulfonated to produce a water-soluble fraction (EPS(GLC)-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction (EPS(GLC)-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where each polysaccharide was eluted as a single symmetrical peak. The structures of the sulfonated and re-sulfonated botryosphaerans were investigated using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FT-IR), and 13C nuclear magnetic resonance (13C NMR) spectroscopies. EPS(GLC) and EPS(GLC)-RS were also assayed for anticoagulation activity, and EPS(GLC)-RS was identified as an anticoagulant.