http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Maria Isabel Rodrigues (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Cultural memories, discursive gaps, and tourism promotion: A framework for applied research
Marcelo G. Oliveira,Maria do Carmo Leal,Maria Isabel Roque,Maria João Forte,Sara Rodrigues de Sousa,Antónia Correia 한국마케팅과학회 2017 마케팅과학연구 Vol.27 No.4
Recent studies about the relation between heritage and tourism have consistently signaled the existence of fractures between the cultural reality of tourism destinations and the cognitive and emotional experience of contemporary visitors. On the other hand, the study of the relations between tourism and literature and the recent valorization of storytelling in tourism promotion have both pointed out the importance of diff erent types of discourses in the constitution of tourism spaces and the shaping of visitors’ expectations and experiences. When the desire for authenticity expressed by cultural tourists is also taken into account, the existence of inconsistencies between the cultural realities of destinations, existing discourses and the expectations and perceptions of tourists becomes apparent. Adapting, in an innovative approach, the Gap Model of Service Quality, this paper intends to present a research framework capable of enlightening existing cultural inconsistencies considering the discourses involved in the promotion and experience of tourism destinations. The framework will be applied in an exploratory investigation of the role of José Saramago’s Baltasar and Blimunda in the tourism promotion of Mafra and Lisbon, Portugal, with the aim of developing a conceptual model capable of describing inconsistencies in the promotion and experience of cultural destinations and facilitating the development of adequate mediation proposals .
-
Ilda P. Ribeiro,Joana M. Rodrigues,Alexandra Mascarenhas,Vanessa Marques,Francisco Caramelo,Maria J. Julião,Thomas Liehr,Joana B. Melo,Isabel M. Carreira 한국유전학회 2019 Genes & Genomics Vol.41 No.10
Background Head and neck squamous cell carcinoma cell lines are useful preclinical models to understand the molecular processes underlying the development of such tumors, and to establish targeted therapies. Objective We performed a comprehensive (cyto)genomic and epigenetic characterization of three new established primary human head and neck squamous cell carcinoma cultures and an established, yet undercharacterized cell line: BICR 10. Methods Karyotyping, multiplex fluorescence in situ hybridization, array comparative genomic hybridization and methylation- specific multiplex ligation-dependent probe amplification were applied. Results The three primary cultures turned out to be a near-triploid and BICR 10 near-diploid. Banding and molecular cytogenetic analysis revealed non-random numerical and structural aberrations. The most common rearrangements identified in BICR 10 cell line were non-complex derivatives of reciprocal translocations, in which the breakpoints often appeared in centromeric/near-centromeric regions. In the 3 primary cell cultures the most common rearrangements observed were iso- and derivatives chromosomes derived from translocations. Overall, gains of 7p, 8q and losses at 3p, 8p, 9p, 18q and Xp were present in all four studied samples. Among the analyzed genes, BICR 10 cell line exhibited enhanced methylation of gene promoter; however, in all studied samples PAX5, WT1 and GATA5 were methylated. Conclusion The here reported comprehensive characterization of BICR 10 cell line and the new established cultures enriches the resources available for head and neck cancer research, especially for testing therapeutic agents.
-
Evaluation of Different Parameters for the Purification of Inulinase Using an Ion Exchange Fixed Bed
Susana J. Kalil,Silvana T. Silveira,Francisco Maugeri-Filho,Maria Isabel Rodrigues 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.4
The main conditions affecting the downstream process for purification of inulinase from Kluyveromyces marxianus var. bulgaricus ATCC 16045 were investigated. Parameters including the elution mode, linear rate, ionic strength, and elution pH were evaluated in order to achieve the best purification factor and recovery. The best purification factor and recovery were achieved in run 6 using a gradient elution mode with concentrations of NaCl ranging from 0 to 1 M, a linear flow rate of 100 cm/h and a pH value of 4.1. Under these conditions, the recovery was ~ 67.5% and the purification factor ~ 6.6-fold.
-
Marcus B. S. Forte,Marcio A. Mazutti,Francisco Maugeri Filho,Maria Isabel Rodrigues 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.6
This work investigates the adsorption of clavulanic acid using natural six cationic forms (Na^+, Ca^(+2),Ba^(+2), Sr^(2+), K^(+), and Mg^(2+)) of the X and NZ zeolites in a stirred tank reactor since the separation is an important step of the biomolecule production. A mathematical model was proposed taking into account the transport of CA molecules from the liquid phase to the surface of the adsorbent and after diffusion into the particles. The estimated kinetic and mass transfer parameters were used to evaluate adsorption rates and mass transfer resistances involved in the separation of clavulanic acid from the broth. It has been shown that mass-transfer phenomena were a limiting step in the clavulanic acid adsorption process and that the adsorption rate should be considered to evaluate the system. Amongst the materials, the synthetic zeolite NaX was selected as the most appropriate material to separate clavulanic acid because this material presented the highest values for the observed reaction rate,compensating for the external mass transfer resistance. Modeling and simulation of clavulanic acid purification using the zeolite NaX showed a satisfactory fitting of experimental data. The model was used to simulate the process and it was evaluated for its technical and economical viability by comparisons considering the influence of the solid:liquid ratio on the adsorption equilibrium time and on the hydrolysed mass of biomolecule.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
