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Rosana Goldbeck,Francisco Maugeri Filho 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.suppl1
The main goal of this work was to select and characterize lipase producing wild yeasts isolated from different biomes of Brazil. A total of 372 wild yeasts were tested for their lipase producing potential and 3 strains were selected and characterized according to their substrate specificity, pH, and temperature effects and biocatalytic capacity for synthesis in organic media. For the AC02strain, the optimal enzymatic conditions were pH 7.0 and temperature 44ºC, whilst for AAV1 strain, pH 7.0 was also better, but the optimal temperature was 47ºC, and for AY3strain the optimal temperature was 37ºC and pH 6.6. About substrate specificity, the lipase from AY3 strain showed higher specificity for short-chain triglycerides. The AC02strain showed the highest lipolytic activity among the 3selected, and was molecularly identified by sequencing the ITS1-5.8S-ITS2 and D1/D2 domains of the subunit (26 S)ribosomal DNA as Metschnikowia pulcherrima.
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Evaluation of Different Parameters for the Purification of Inulinase Using an Ion Exchange Fixed Bed
Susana J. Kalil,Silvana T. Silveira,Francisco Maugeri-Filho,Maria Isabel Rodrigues 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.4
The main conditions affecting the downstream process for purification of inulinase from Kluyveromyces marxianus var. bulgaricus ATCC 16045 were investigated. Parameters including the elution mode, linear rate, ionic strength, and elution pH were evaluated in order to achieve the best purification factor and recovery. The best purification factor and recovery were achieved in run 6 using a gradient elution mode with concentrations of NaCl ranging from 0 to 1 M, a linear flow rate of 100 cm/h and a pH value of 4.1. Under these conditions, the recovery was ~ 67.5% and the purification factor ~ 6.6-fold.
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Marcus B. S. Forte,Marcio A. Mazutti,Francisco Maugeri Filho,Maria Isabel Rodrigues 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.6
This work investigates the adsorption of clavulanic acid using natural six cationic forms (Na^+, Ca^(+2),Ba^(+2), Sr^(2+), K^(+), and Mg^(2+)) of the X and NZ zeolites in a stirred tank reactor since the separation is an important step of the biomolecule production. A mathematical model was proposed taking into account the transport of CA molecules from the liquid phase to the surface of the adsorbent and after diffusion into the particles. The estimated kinetic and mass transfer parameters were used to evaluate adsorption rates and mass transfer resistances involved in the separation of clavulanic acid from the broth. It has been shown that mass-transfer phenomena were a limiting step in the clavulanic acid adsorption process and that the adsorption rate should be considered to evaluate the system. Amongst the materials, the synthetic zeolite NaX was selected as the most appropriate material to separate clavulanic acid because this material presented the highest values for the observed reaction rate,compensating for the external mass transfer resistance. Modeling and simulation of clavulanic acid purification using the zeolite NaX showed a satisfactory fitting of experimental data. The model was used to simulate the process and it was evaluated for its technical and economical viability by comparisons considering the influence of the solid:liquid ratio on the adsorption equilibrium time and on the hydrolysed mass of biomolecule.
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