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Pharmacognostic Evaluation of Pipper longum Linn. Fruit
Manjoosha Srivastava,Meenu Gupta,Sharad Srivastava,Shanta Mehrotra,Vinita Sharma,AKS Rawat 한국생약학회 2007 Natural Product Sciences Vol.13 No.2
present study includes macro and microscopical details, powder study, physico-chemical studyand HPTLC fingerprinting of the Piper longum fruits. Microscopic studies revealed the presence of stone cells,starch grains and thin walled fragments of parenchymatous cells. Physico-chemical studies showed alcohol andether soluble extract 24.53 and 6.7, sugar 0.35, starch 21.33 and tannin 0.83% respectively. Successive soxhletextract showed maximum percentage of hexane soluble fraction i.e. 22.52. The HPTLC profile has also beenperformed against the reference marker piperine, which was identified at Rf 0.42. pharmacognostical evaluation of fruit has been undertaken.KeywordsPiper longum, Pharmacognosy, HPTLC.
Pharmacognostic Evaluation of the Roots of Berberis chitria Lindl.
Sharad Kumar Srivastava,Ajay Kumar Singh Rawat,Manjoosha Srivastava,Shanta Mehrotra 한국생약학회 2006 Natural Product Sciences Vol.12 No.1
chitria (family Berberidaceae) has a close affinity with B. aristata, used in traditionalsystems of medicine as a drug ‘Daruharidra’ for skin disease, jaundice, affection of eyes, and rheumatism.Keeping this in view, in the present study attempts have been made to identify marker characters of B. chitria inorder to differentiate the two species. Some of the diagnostic features of the root are patches of pericyclic fibre,pitted sclerieds and berberine containing cells and heterocyclic medullary rays. Besides, the physicochemicalcharacters such as total ash; acid insoluble ash; alcohol and water soluble extractive; tannins; sugar and starchpercentages has shown variations. The percentage of berberine as berberine hydrochloride was also calculatedB. aristata and B. asiatica i.e. 3.16%.Thus, this species can be utilized as a possible substitute to Daruharidra.KeywordsBerberis chitria, HPTLC, Daruharidra, substitute