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Manases González-Cortázar,José E Sánchez,Magaly Huicochea-Medina,Victor M Hernández-Velázquez,Pedro Mendoza-de-Gives,Alejandro Zamilpa,Ma Eugenia López-Arellano,Jesús Antonio Pineda-Alegría,Liliana Ag 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.3
Sheep haemonchosis is a disease that causes anemia, diarrhea, weight loss, low production, and even death of young animals. This represents a negative economic impact on the livestock sector. For decades, chemicals have been used to control this parasitic disease; however, cases of anthelmintic resistance have increased around the world. For this reason, the search for control alternatives is necessary. Several studies have shown that edible mushrooms of Pleurotus genus have different medicinal properties, including nematicidal activity. In this study, the chromatographic fractionation of the hydroalcoholic extract of fruiting bodies Pleurotus djamor was carried out to identify and isolate the metabolites responsible for nematicidal activity. From the fractionation of the extract, the fraction PdR2 and the subfraction PdB were obtained, which were evaluated against Haemonchus contortus under in vitro conditions. The nematicidal effect of the fraction PdB in gerbils infected artificially with H. contortus was evaluated. Finally, the analysis of the PdB fraction by gas chromatography-mass spectrometry and nuclear magnetic resonance was carried out. The results showed that the PdB had 100% of egg hatching inhibition from 5 mg/mL. Regarding larvicidal activity, it presented >97.2% from 24 h to 20 mg/mL. The in vivo evaluation of the PdB fraction showed a reduction of H. contortus larvae of 92.56%. The compounds present in this fraction were the mixture of allitol and an unidentified terpene in a ratio of 9:1. The PdB fraction is a potential alternative for the control of H. contortus, where allitol and a terpene could be responsible for nematicidal activity.
Nematicidal Effect of Shiitake (Lentinula edodes) Extracts Against Haemonchus contortus
Jesús Antonio Pineda-Alegría,José E Sánchez,Elsa Ventura-Zapata,Manases González-Cortazar,Liliana Aguilar-Marcelino 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.9
During the previous decades, the indiscriminate use of anthelmintics for the control of gastrointestinal nematodes has generated anthelmintic resistance in different parts of the world. It is necessary to search for new sustainable control alternatives, such as the use of extracts from plants and edible mushrooms. Therefore, the aim of the present study was to evaluate the nematicidal activity of extracts and fractions of the edible mushroom Lentinula edodes against Haemonchus contortus eggs and infective larvae. The basidiomata of L. edodes ECS-401 were provided by the Tropical Fungi Laboratory of El Colegio de la Frontera Sur and were extracted with ethyl acetate, methanol, and water. Fractionation of the most active extract was carried out by open column chromatography. The bioassays were performed in 96-well microtiter plates using 100 eggs/larvae, a final volume of 100 μL, and different concentrations of extracts/fractions (n = 4). Bioassay readings were taken at 48 h for egg hatching inhibition (EHI) and at 24, 48 and 72 h for larval mortality (LM). The ethyl acetate extract exhibited the highest percentage of EHI (100%). For the LM bioassay, the aqueous extract was the most active (69%), but its fractions did not show larvicidal activity. The chemical profile of the aqueous extract was analyzed by high-performance liquid chromatography, which showed the presence of phenols, flavonoids, and terpenes. L. edodes extracts showed ovicidal and/or larvicidal activity.
Jesus Antonio Pineda-Alegrıa,Jose Ernesto Sa´nchez-Vazquez,Manases Gonzalez-Cortazar,Alejandro Zamilpa,Marıa Eugenia Lopez-Arellano,Edgar Josue Cuevas-Padilla,Pedro Mendoza-de-Gives,Liliana Aguilar-Ma 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.12
Pleurotus djamor is an edible mushroom that has medicinal properties. This study aimed to assess the in vitro activity of P. djamor extracts and fractions against Haemonchus contortus eggs and exsheathed infective larvae (L3). Crude hydroalcoholic extracts were obtained by maceration and fractions were obtained through chromatography. Metabolite identity was determined using gas chromatography/mass spectrometry (GC-MS) analysis. The results showed that P. djamor extracts had no significant activity against eggs at the concentrations used. However, the extract showed 98.7% and 77% larval mortality 72 h postconfrontation at 320 and at 160 mg/mL, respectively. The chromatography analysis resulted in 23 fractions that were eventually grouped into three fractions (E1, E2, and E3). These fractions showed the following egg hatching inhibition percentages: E1 = 100, E2 = 38.7, and E3 = 5.5 at 10 mg/mL concentration 72 h postexposure. Likewise, larval mortality percentages after this period were 90.6, 100, and 0.44 at 40 mg/mL (P < .05), respectively. The GC-MS showed five major compounds in E1 fraction, including four fatty acids: (i) pentadecanoic, (ii) hexadecanoic, (iii) octadecadienoic, (iv) octadecanoic acid, and one terpene identified as β-sitosterol. We concluded that the edible mushroom P. djamor possesses nematicidal metabolites, which could be used as an alternative anthelmintic treatment.
Lichenoid Reaction - A Diagnostic Dilemma
Manas Bajpai 대한예방치과학회 2013 International Journal of Clinical Preventive Denti Vol.9 No.1
Oral lichenoid reactions are considered as a variant of Oral Lichen Planus (OLP) may be regarded as a disease by itself or as an exacerbation of an existing OLP, by the presence of medication and dental materials. The management of OLP and the Various OLLs may be different. Therefore, accurate diagnosis should be aimed at; here we are presenting a case of a white lesion in a fifty year old male patient shows histopathological features similar to with OLP. The diagnosis was made on the ground of the drug association.
Manas Bajpai 대한임상예방치과학회 2011 International Journal of Clinical Preventive Denti Vol.7 No.4
Ghost cell is a dead cell in which the cell outline remains is visible, but whose nucleus and cytoplasmic structures are not stainable Formerly, ghost cell have been considered to represent a form of true or aberrant keratinization simple cell degeneration or enamel matrix but the true nature of these cells has not been determined. Ghost cell maybe due to abnormal keratinization, related to ischemia, coagulative necrosis. Calcification may occur in some of the ghost cell and represent dystrophic calcification the aim of this paper is an attempt to describe in detail about the origin, microscopic appearance and histopathologic features of ghost cells.
Quadcopter control using the viscoelastic control law
Manas Kumar Sahoo,J. K. Dutt,S. K. Saha 제어로봇시스템학회 2021 제어로봇시스템학회 국제학술대회 논문집 Vol.2021 No.10
Quadcopter is an unmanned aerial vehicle (UAV) with four rotors and generally, they are placed in a square formation with equal distance from the centre of mass. The thrust produced by each rotor is directed upward and the UAV is controlled by varying the magnitude and direction of the rotor speed with the help of DC motors. In this paper, a novel controller is proposed that imitate the behavior of four-element viscoelastic material. Some unmodelled disturbances was fed to the system to check the controller performance. The FE controller performance was compared with the classical PID controller. As in real world performances quadcopters are used in the presence of various sensors and other measuring devices, the time delay introduced by these instruments becomes very important for the stability of the system. Hence both the controllers were subjected to some designed time delay so that they can be compared in real-world scenario. The simulation of the above was carried out with the help of Simulink platform and from the simulation it was found that the FE controller is fast, accurate and precise in performance compared to the PID controller.
Inflammatory Myofibroblastic Tumor of the Urinary Bladder Managed by Laparoscopic Partial Cystectomy
Manas Ranjan Pradhan,Priyadarshi Ranjan,Ram Nawal Rao,Saurabh Sudhir Chipde,Krishna Pradhan,Rakesh Kapoor 대한비뇨의학회 2013 Investigative and Clinical Urology Vol.54 No.11
Inflammatory myofibroblastic tumor of the urinary bladder is a rare mesenchymal tumor with uncertain malignant potential. It often mimics soft tissue sarcomas both clinically and radiologically. Surgical resection in the form of partial cystectomy or transurethral resection remains the mainstay of treatment. Herein we report the case of an inflammatory myofibroblastic tumor in a young girl, which was managed by laparoscopic partial cystectomy. To the best of our knowledge, this is the first reported case of laparoscopic management of an inflammatory myofibroblastic tumor of the urinary bladder.