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Navale Prakash M,Manamohan Maligeppagol,Asokan R.,Sharath Chandra G.,Prasad Babu K.,Latha J.,Krishna Kumar N.K.,Ellango R.,Krishna V. 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
Helicoverpa armigera is an important pest infesting a number of crops of high commercial value leading to huge economic losses globally. RNA interference (RNAi) is a potent tool for the control of insect pests and towards this objective, transgenic tomato expressing dsRNA of Juvenile hormone acid methyl transferase (JHAMT) gene of H. armigera was developed. The H. armigera larvae fed on HaJHAMT tomato leaves led to a severe (90%) down regulation of the cognate gene expression thus adversely affected the feeding and metamorphosis. Reduction in larval and pupal weight and inability to undergo complete metamorphosis were observed that led to larval-pupal intermediates and subsequently affected the eclosion. Thus, JH down regulation is an attractive target due to specificity to insects and its important role in insect growth and development to engineer insect resistance in crops using RNAi.