糖의 添加에 따른 11α-Hydroxylase의 活性에 關한 考察

金末南 상명대학교 논문집 1985 상명대학교논문집 Vol.15 No.-

The effects of various sugars on the reaction rate of progesterone transformation were investigated using Atpergillus Phoenicia whole cell as enzymes source. The addition of glucose in the reaction medium accelerated considerably the reaction rate, so that the reaction time required for attainment of the limiting value of the 11α-hydroxyprogesterone yield reduced from 40 to 10 hours with increase in glucose concentration, The excess glucose addition, however, did not activate the reaction any more. Fructose 1, 6-diphosphate and pyruvic acid which are intermediate metabolites of glycolysis, and disaccharides such as lactose and maltose also speeded up the reaction rate, but with less effectiveness than glucose. Contrary to expectation, xylose transformed progesterone more rapidly than glucose. However, the former produced larger quantities of dihydroxyprogesterone than the latter.

MOS 소자의 移動性 陽이온들의 電氣的 特性

金末文,南尙熙 嶺南大學校附設 基礎科學硏究所 1985 基礎科學硏究 Vol.5 No.-

Thermally stimulated Ionic Current (TSIC) measurements have been used to study the kinetic behavior of mobil ions in Al-SiO₂-Si Structures formed by evaporation of Al electrodes onto thermally oxidized Si wafer. It is shown that three distinct peaks were observed in the temperature range 30~350℃. The first peak at about 100℃, and the second peak at about 200℃, and the third peak at about 350℃. The first peak is to the motion of ??, wheras the second peak results from the motion of ??, the third peak is to the motion of ??. It is shown that the ionic current is limited primarily by release of mobil ions from traps at the interface. This model is proposed which accurately predict the detrapping rate for an arbitary temperature-time profile and change of voltage. Its parameters are the initial distribution of ions among the trapping states, ??, and heating rate β, characterizing the attempt-to-escape frequency, and that trapped mobil ions release from trap. Activation energy, E canbe derived from analysis of a TSCI curve. Activatron energy is to the results of 0.52 for ??, and 0.81 for ??. The results is shallow trap for ??, and deep trap for ??. A value for β can be determined by performing experiment in the TSIC stages. Increase of peak current was caused by the increase of β. Changes of capacitance-voltage(C-V) characterics by Bias-Temperature(B-T) stresses have been measured for detecting mobil ions.

자동판매기 기호식품에서의 미생물 분포

김말남,허희선,서정희 상명대학교 가정문화연구소 1992 가정문화연구 Vol.9 No.-

1991년 9월부터 11월에 걸쳐 상명여자대학교 서울 캠퍼스의 학생회관 2층, 도서관 2층 및 남관에 설치되어 있는 커피 자동판매기를 대상으로 블랙 커피, 프림 커피, 밀크 커피에서 검출되는 세균의 종류 및 생균수를 조사하였다. 일반 세균으로는 Neisseria lactamica, Acinetobacter calcoaceticus, Flavimonas oryzihabitans, Lactobacillus sp. 및 Pediococcus sp.가 검출되었으며, 대장균군과 살모넬라균에 속하는 세균은 발견되지 않았다. 일반 세균의 생균수는 블랙 커피에서 7.0×10 exp(5)-6.2×10 exp(6)cells/㎖, 프림 커피에서 8.0×10 exp(5)-6.4×10 exp(6)cells/㎖, 밀크 커피에서 1.1×10 exp(6)-7.0×10 exp(6)cells/㎖ 수준으로 나타났다. 이상에서 교내의 특정공간에 설치되어 있는 커피 자동판매기의 커피 제품에는 상당수에 달하는 여러 종류의 일반 세균이 검출되어 철저한 위생적 관리가 필요하다고 판단된다. Bacteriological infection level was investigated for black coffee, frime coffee and milk coffee for automatic vending machines installed on 2nd floor of the Students' Hall, of the Library and of Namkwan in Seoul campus of Sangmyung Women's University from September till November of 1991. Viable cells such as Neisseria lactamica, Acinetobacter calcoaceticus, Flavimonas oryzihabitans, Lactobacillus sp. and Pediococcus sp. were detected, while bacteria belonging to Coliform or Salmonella group were not isolated. Number of viable cells counted to be 7.0 × 10 exp(5)-6.2 × l0 exp(6) cells/㎖ in black coffee, 8.0 × 10exp(5)-6.4 × 10 exp(6)cells/㎖ in frime coffee and 1.1 × 10 exp(6)-7.0 × l0 exp(6)cells/㎖ in milk coffee. From these results, it can be concluded that coffee of automatic vending machines is significantly infected by various viable bacteria and that a thorough hygienic control is needed.

Poly(3-hydroxybutyrate) 분해세균의 선택적 분리

김말남 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.4

탄소원과 에너지원으로 PHB를 이용하는 세균을 난지하수종말처리장의 활성오니로부터 분리하여 Corynebacterium jeikeium A Kej 8로 동정하였다. 27℃, 37℃ 및 60℃에서의 PHB 생분해성을 비교하였을 때 37℃에서 분해가 가장 빠르게 진행되었고, 60℃에서는 분해가 거의 일어나지 않았다. 각 폴리에스테르에 대한 C. jeikeium A KEJ 8의 생분해성을 변형 Sturm test법으로 조사한 결과는 반응 30일째에 PHB는 36.4%, PLA는 21.5%, PHB/HV 5%는 33.7%, PHB/HV 11%는 37.2%의 생분해도를 나타내었다. 생분해 후의 종합체 필름 표면의 형태 변화를 SEM으로 조사하였다. A bacteria capable of degrading and utilizing PHB as sole carbon and energy source was isolated from the activated sludge of Nanji sewage water treatment plant. The strain was identified as Corynebacterium jeikeium A KEJ 8. Biodegradability of PHB was measured at 27℃, 37℃ and 60℃ and it degraded faster at 37℃ than at the two other temperatures. Biodegradability of PBH, PLA, PHB/HV 5% and PHB/HV 11% by C. jeikeium A KEJ 8 was investigated using the modified Sturm test. The degradation of PHB, PLA, PHB/HV 5% and PHB/HV 11% for 30 days by the isolated strain showed 36.4%, 21.5%, 33.7% and 37.2% respectively. The morphology of polymer surface film after biodegradation was observed by SEM.

PHB 분해효소를 생산하는 세균의 분리

김말남 상명대학교 기초과학연구소 1997 기초과학연구 Vol.10 No.-

탄소원과 에너지원으로 PHB를 이용하는 세균을 난지 하수종말처리장에서 채취한 활성오니로부터 분리하였다. PHB 분해능이 가장 우수한 균주 1주를 선택하여 Corynebacterium pseudodiphtheriticum KEJ 7로 동정하였다. C. pseudodiphtheriticum KEJ 7에 의한 PHB, PHB/HV 5%, PHB/HV 1% 및 PLLA의 생분해성을 변형 Sturm test 법으로 조사하였을 때 PHB 및 PHB/HV copolymer는 34-36%의 분해도를 나타내었으며, HV의 함량에 따라 약간의 분해도 차이를 보였다. 이에 비하여 PLLA는 28% 정도로 비교적 낮게 분해되었다. 27℃, 37℃ 및 60℃로 온도를 달리하여 PHB 생분해성을 조사한 결과 37℃에서 분해능이 비교적 높게 나타났다. A bacteria capable of degrading and utilizing PHB as sole carbon and energy source was isolated from the activated sludge of Nanji sewage water treatment plant. One strain, exhibiting the most effective capability for the PHB degradation, was identified as Corynebaderium pseudodiphtheriticum KEJ 7. Biodegradability of PHB, PHB/HV 5%, PHB/HV 11% and PLLA by C. pseudodiphtheriticum KEJ 7 was investigated using the modified Sturm test. The degradation of PHB and PHB/HV copolymers by the isolated strain showed 34-36% of biodegradability, and it depended upon the HV content of the polymers. On the other hand, PLLA showed 28% of biodegradability. Biodegradability of PHB was measured at 27℃, 37℃, and 60 C and it degraded faster at 37 C than at two other temperatures.

Sterol 측쇄 분해능을 가진 토양세균의 분리

김말남,박희정 상명대학교 자연과학연구소 1995 自然科學硏究 Vol.2 No.-

Aspergillus phoenicis Whole Cell 의 β-Galactosidase 활성에 관한 연구

김말남 한국균학회 1983 韓國菌學會誌 Vol.11 No.3

β-Galactosidase activity of Aspergillus phoenicis was studied using ONPG and lactose as substrate. It increased monotonically during the exponential growth phase and dropped rapidly at the beginning of the stationary one. It exhibited high tolerable temperature and acidic optimal pH which provides certain advantages from the industrial view point. Enzyme of β-galactosidase had more subsrate affinity for ONPG than for lactose and its apparent maximum activity was also higher with the former as substrate. Activity of this enzyme depended upon the conditions of immobilization. Optimum crosslinking reaction was occurred at pH 7.2 and 0.35 vol. % of glutaraldehyde concentration.

활성오니로 부터 분리한 PHB 분해세균의 특성

김말남 상명대학교 기초과학연구소 1996 기초과학연구 Vol.9 No.-

PHB를 유일한 탄소원 및 에너지원으로 사용하는 세균 5주를 서울시 소재 중랑천 하수처리장의 활성오니로부터 분리하였다. PHB 생분해도가 가장 높은 균주 1주를 선택하여 그 특성을 조사한 결과 Gram 음성의 구균(1㎛)으로 PHB를 보강한 무기염배지와 비교할 때 복합배지에서 배양하였을 때에 약 100배 이상 더 많이 성장하였고 항생제 ampicillin, cephalothin 및 streptomycin에 내성을 나타내었다. 여러 생화학적 검사를 실시한 결과 이 균주를 Enterobacter cloacae FC-101로 동정하였다. Five bacteria capable of degrading and utilizing PHB as sole carbon and energy source were isolated from the activated sludge of the wastewater treatment plants at Seoul Jungrangchun. One strain, exhibiting the most effective capability for PHB degradation, was characterized to be a gram negative coccus. The strain grown in rich medium was 100 times as numerous as that cultivated in mineral medium supplemented with PHB. It showed resistance to antibiotics such as ampicillin, cephalothin and streptomycin, and was identified as Enter-obacter cloacae FC-101 by the biochemical tests.

통영 연안 해역에서의 미생물 분포

장지철,김말남,이진환,김종만 한국환경생물학회 2000 환경생물 : 환경생물학회지 Vol.18 No.4

Surface and bottom water samples were collected from 10 stations located in the coastal area of Tongyong in April, August and October 2000. Distribution of heterotrophic bacteria, coliform bacteria and fungi in the sea water samples was investigated by measuring the comrresponding viable cell number according to the plate counting method. Heterotrophic bacteria in the surface water counted 3.1×10 exp∼4.0×10 exp (3) cfu ml^1, 2.7×10 exp∼1.2×10 exp (5) cfu ml^-1 and 1.3×10 exp (2)∼7.2×10 exp (2) cfu ml^1 in April, August and October, respectively. The cell number of coliform bacteria in the surface water amounted to 0∼15×10 exp (1) cfu ml^-1, 3.5×10 exp (1)∼5.2×10 exp (3) cfu ml^-1 and 0∼1.8×10 exp (2) cfu ml^-1 in April, August and October, respectively. As for fungi, the cell number in the surface water was 0∼3.0×10 exp (1) propagules ml^-1, 3.0×10 exp (1)∼8.0×10 exp (1) propagules ml^-1 and 0∼2.2×10 exp (1) propagules ml^-1 in April, August and October respectively. The surface water samples from the station 3 in August were added with feed staffs for fish as much as 0.01gl^-1, 0.1gl^-1 and 1gl^-1 and cultured at 5℃, 15℃, 25℃ and 35℃. Microbial cells were not isolated at all when the culturing temperature was 5℃. However, the microbial cell number increased significantly in all the surface water samples containing 1gl^-1 of the feed stuffs when cultured at 15℃, 25℃ and 35℃.

Adsorption of $\textrm{Pb}_{2+}$ in the components of bacterial cell membrane

Kim, Mal-Nam The Microbiological Society of Korea 1995 The journal of microbiology Vol.33 No.4

S. epidermidis cell was fractionated into cell wall, cell membrane and cytoplasm. The cell membrane adsorbed the most abundant $\textrm{Pb}_{2+}$ per unit dry weight of the three fractions tested. Adsorption behavior of $\textrm{Pb}_{2+}$ in lipid and protein, which are the main components of the cell membrane, indicated that phosphatidylethanolamine and phosphatidylinositol having phosphoryl group and gangliosides containing carboxyl groups adsorbed much more $\textrm{Pb}_{2+}$ than triglycerides lacking any chargeable functional groups. Protein purified from cell membrane adsorbed larger amount of $\textrm{Pb}_{2+}$ than total native cell membrane or cell membrane lipid.