Molecular Characterization of Selected Local and Exotic Cattle Using RAPD Marker

Khatun, M. Mahfuza,Hossain, Khondoker Moazzem,Rahman, S.M. Mahbubur Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

In order to develop specific genetic markers and determine the genetic diversity of Bangladeshi native cattle (Pabna, Red Chittagong) and exotic breeds (Sahiwal), randomly amplified polymorphic DNA (RAPD) analysis was performed using 12 primers. Genomic DNA was extracted from 20 cattle (local and exotic) blood samples and extracted DNA was observed by gel electrophoresis. Among the random primers three were matched and found to be polymorphic. Genetic relations between cattle's were determined by RAPD polymorphisms from a total of 66.67%. Statistical analysis of the data, estimating the genetic distances between cattle and sketching the cluster trees were estimated by using MEGA 5.05 software. Comparatively highest genetic distance (0.834) was found between RCC-82 and SL-623. The lowest genetic distance (0.031) was observed between M-1222 and M-5730. The genetic diversity of Red Chittagong and Sahiwal cattle was relatively higher for a prescribed breed. Adequate diversity in performance and adaptability can be exploited from the study results for actual improvement accruing to conservation and development of indigenous cattle resources.

Optimal control strategy to combat the spread of COVID-19 in absence of effective vaccine

M.H.A. Biswas,M.S. Khatun,M.A. Islam,S. Mandal,A.K. Paul,A. Ali 한국전산응용수학회 2022 Journal of applied mathematics & informatics Vol.40 No.3

Many regions of the world are now facing the second wave of boomed cases of COVID-19. This time, the second wave of this highly infectious disease (COVID-19) is becoming more devastating. To control the existing situation, more mass testing, and tracing of COVID-19 positive individuals are required. Furthermore, practicing to wear a face mask and maintenance of physical distancing are strongly recommended for everyone. Taking all these into consideration, an optimal control problem has been reformulated in terms of nonlinear ordinary differential equations in this paper. The aim of this study is to explore the control strategy of coronavirus-2 disease (COVID-19) and thus, minimize the number of symptomatic, asymptomatic and infected individuals as well as cost of the controls measures. The optimal control model has been analyzed analytically with the help of the necessary conditions of very well-known Pontryagin’s maximum principle. Numerical simulations of the optimal control problem are also performed to illustrate the results.

Genetic diversity and geographic distribution of the <i>Bemisia tabaci</i> species complex in Bangladesh

Khatun, M.F.,Jahan, S.M.H.,Lee, Sukchan,Lee, Kyeong-Yeoll Elsevier 2018 Acta Tropica Vol.187 No.-

<P><B>Abstract</B></P> <P> <I>Bemisia tabaci</I> (Gennadius) is a species complex consisting of at least 40 cryptic species. Although the genetic diversity of <I>B. tabaci</I> has been studied in various regions, little is known about distribution in Bangladesh, which is covered by the Bengal delta, the largest delta on Earth. We conducted an extensive survey throughout the country and determined the nucleotide sequence of mitochondrial cytochrome <I>c</I> oxidase subunit 1 (COI) from 110 individuals. We then examined phylogenetic relationships. The results identified four cryptic species that expressed distinct interspecific variation but low intraspecific variation. Asia I was the most abundant, both Asia II 1 and Asia II 5 were moderately abundant, and Asia II 10 was found only in the central region. COI sequences of each cryptic species were distinctive and differentiated into many haplotypes. Our study provides important information to better understand the genetic diversity and geographic distribution of cryptic species in Bangladesh and nearby countries.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genetic diversity of <I>Bemisia tabaci</I> was determined in Bangladesh. </LI> <LI> We identified four indigenous cryptic species but not MEAM1 and MED invasive cryptic species. </LI> <LI> Asia I was abundant, both Asia II 1 and Asia II 5 were moderate, and Asia II 10 was found only in the central region. </LI> <LI> Our study provides important information on the genetic diversity and geographic distribution of <I>B. tabaci</I> in Bangladesh. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Thermosensitive hexanoyl glycol chitosan-based ocular delivery system for glaucoma therapy

Cho, I.S.,Park, C.G.,Huh, B.K.,Cho, M.O.,Khatun, Z.,Li, Z.,Kang, S.W.,Choy, Y.B.,Huh, K.M. Elsevier BV 2016 ACTA BIOMATERIALIA Vol.39 No.-

Conventional eye drops quickly move away from the surface of the eye; as a result, ocular bioavailability is very limited. To overcome this issue, we developed a thermosensitive hexanoyl glycol chitosan (HGC) as a carrier for topical drug delivery to the eye. Here, we modulated the degree of N-hexanoylation to control the thermogelling behavior and prepared a new ocular formulation of HGC for glaucoma therapy. The viscosity of the aqueous formulation sharply and significantly increases at body temperature. The results from cytotoxicity evaluation showed that HGC is non-toxic at up to 1.25wt.%. In vivo experiments demonstrated that HGC is maintained on the preocular surface for a comparatively longer period of time due to its enhanced viscosity at body temperature. As a result, when brimonidine was loaded, the formulation exhibited attractive bioavailability properties as well as more prolonged period of lowered intra-ocular pressure (14h) compared with Alphagan P, the marketed medication for brimonidine treatment. Statement of Significance: In this manuscript, hexanoyl glycol chitosan (HGC) was synthesized by the N-hexanoylation of glycol chitosan. We have observed that an aqueous solution of HGC exhibited a dramatic increase in viscosity as the temperature increased. The HGC-based formulation showed prolonged retention on the preocular surface and enhanced drug availability and efficacy.

Comparison of markers predicting litter size in different pig breeds

Kwon, W.-S.,Rahman, M. S.,Ryu, D.-Y.,Khatun, A.,Pang, M.-G. Wiley (Blackwell Publishing) 2017 Andrology Vol.5 No.3

<P>To overcome the limitations of conventional analysis of male fertility in animals and humans, proteomic studies have been performed to develop fertility-related biomarkers for prognosis and diagnosis of male fertility. However, the studies were focused on specific species or breeds. Therefore, a study is required to validate whether fertility-related markers would apply to other breeds in pigs. In this study, previously developed fertility-related biomarkers from Landrace were validated to use for prognosis of male fertility in commercially available breeds. Expression level of eight biomarkers in non-capacitated and capacitated (C) spermatozoa from Yorkshire and Duroc boars was analyzed. And then, to explore the validity of these markers for prognosis of male fertility, i.e. litter size, artificial insemination was performed. Among them, RAB2A (NC) and UQCRC1 (NC) turned out to be highest efficient markers for Yorkshire. RAB2A (C) was most efficient marker for Duroc. Average litter size has increased as much as 1.41 live born after prediction using eight fertility-related biomarkers in Yorkshire. In addition, average 2.52 litter size was increased after prediction using eight fertility-related biomarkers in Duroc. Average litter sizes were especially highly increased after prediction of fertility using RAB2A (NC) in Yorkshire (1.57 piglets) and TPI (NC) in Duroc (3.14 piglets), respectively. As a result, all biomarkers were significantly correlated with litter size. However, overall accuracy to predict litter size in three breeds was different in response with each marker. Average litter size after artificial insemination was also significantly affected by marker selection. Therefore, this study suggests that developed fertility-related markers may be used for prognosis and diagnosis of male fertility irrespective of breed. However, selection of efficient markers for breeds should be considered to obtain more accurate and efficient outcomes.</P>

Effect of polymorphisms in the GBP1, Mx1 and CD163 genes on host responses to PRRSV infection in pigs

Niu, P.,Shabir, N.,Khatun, A.,Seo, B.J.,Gu, S.,Lee, S.M.,Lim, S.K.,Kim, K.S.,Kim, W.I. Elsevier Scientific Pub. Co 2016 Veterinary microbiology Vol.182 No.-

<P>Porcine reproductive and respiratory syndrome (PRRS) is the most economically important disease to the swine industry, and effective prevention strategy for this disease is still required. Guanylate-binding protein 1 (GBP1) and myxovirus resistance protein 1 (Mx1) are two important proteins belonging to the GTPase superfamily that have been previously described to show antiviral effects. CD163 is considered the most important receptor for PRRSV attachment and internalization. Therefore, the aim of the present study was to evaluate the effects of these genes on host resistance against PRRSV infection in conjunction with the host immune response following PRRSV challenge. The results showed that pigs with AG genotype for the GBPI exon2 exhibited a significantly higher average daily weight gain (ADWG) and lower average viremia than AA or GG genotype. Furthermore, pigs harbouring the AG genotype for the GBP1 gene presented greater CD4(+)CD25(+) and CD8(+)CD25(+) T cell populations at 4 and 18 days post challenge (dpc), respectively, as compared with other genotypes whereas pigs with CC genotype for the CD163 gene displayed significantly higher nucleocapsid-specific antibody titers at 11 dpc. However, pigs with a single 11-bp deletion or insertion in the Mx1 gene did not show significant differences in either weight gain or viremia. Based on these results, we concluded that GBPI is most significantly associated with resistance against PRRSV infection and efficient T cell activation in pigs. (C) 2015 Elsevier B.V. All rights reserved.</P>

Global Spread of Begomoviruses via whiteflies and a new route via plant seeds

Kyeong-Yeoll Lee,Jae-Kyoung Shim,M.S. Fatema Khatun,Pijush Kanti Jhan,Hwal-Su Hwang,Sukchan Lee 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

Global Spread of Begomoviruses via whiteflies and a new route via plant seeds

Kyeong-Yeoll Lee,Jae-Kyoung Shim,M.S. Fatema Khatun,Pijush Kanti Jhan,Hwal-Su Hwang,Sukchan Lee 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10

Tomato yellow leaf curl virus (TYLCV) and its vector insect, the sweet potato whitefly Bemisia tabaci, are major threats to tomato and pepper production in all around world. Since the last three decades, both B. tabaci and TYLCV have been invaded into many countries via different routes. Our studies showed that various geminiviruses including TYLCV can be transmissible by seeds as well as whiteflies. Furthermore, commercially developed resistant tomato strains against TYLCV infection can serve as TYLCV reservoirs and potentially influence on TYLCV epidemics. Therefore, transmission pathways through both insect vectors and seeds should be concerned for suitable management of geminiviruses and whiteflies.