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      저자 : M. W. Jackwood (검색결과 1 건)
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      • Assessment of Replication and Virulence of Attenuated Pseudorabies Virus in Swine

        T. J. Newby,D. P. Carter,K.-J. Yoon,M. W. Jackwood,P. A. Hawkins 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.2

        A nonclinical study was conducted to characterizethe replication behavior of a modified live gE-deletedpseudorabies virus (PRV MS+1) in swine and potentialfor reversion to virulence after animal passages.Twoto 3 week-old weaned pigs, negative for PRV, weremaintained in isolation and challenged by intranasalinstillation.For the first passage,6 pigs were given 1mL of PRV MS+1 (107.3 TCID50/mL) an d 2 w erenecropsied at 3, 4 and 5 days post-inoculation (PI).Brain and secondary lymphoid tissues were collected,homogenized and the supernatants individually pooledfor virus isolation,and PRV was recovered from eachsample. No clinical signs of PRV in fection w ereobserved, but each pig had a nasal swab suspect orpositive for PRV.For the second passage,5 pigs weregiven 1 mL of the homogenate of mixed tissues from1 animal in the previous passage (PRV at 101.9TCID50/mL). At 5 days PI, all pigs were necropsied,and PRV w as n ot recovered from their tissu ehomogenates or nasal swabs, and no clinical signswere observed. During a second attempt at a secondpassage,tissue homogenates from all pigs in the firstpassage (PRV at approximately 101.7 TCID50/mL) werepooled and used to inoculate 15 pigs with 2 mL for 3consecutive days.Ten pigs were monitored for clinicalsigns and seroconversion through 21 days PI, and 5pigs were necropsied at 5 days PI.No clinical signs orPRV antibodies were detected in the 10 monitoredpigs,and no PRV was recovered from the homogenatesor nasal swabs of the 5 necropsied pigs. Thus, noevidence of reversion to virulence was demonstratedin pigs given the attenuated PRV.

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