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      • KCI등재후보

        Relationship of Nitrate Reductase Activity to Leaf Yield, Protein, Sugar and Physiological Attributes in Mulberry (Morus alba L.)

        ( M. K. Ghosh ),( B. K. Das ),( C. Das ),( A. K. Mishra ),( P. K. Mukherjee ),( S. Raje Urs ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.1

        Ten improved mulberry varieties (Vl, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year. The nitrate reductase (EC No. 1.6.6.1) activity (NRA, μmol NO2-h-1 g-1 fr, wt.), total soluble protein (mg g-1 fr, wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture %, unit leaf area (㎠), specific leaf weight (g ㎝-2), total soluble sugar (mg g-1 fr, wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, μ㏖ m-2 g-1) were also studied which showed to vary significantly in tested varieties. Among them, S1635, haying higher NRA (13.25 μ㏖ NO2-h-1g-1 fr, wt.), total soluble protein (39.63 mg g-1 fr, wt.), NPR (16.66 μ㏖ m-2 S-1), total soluble sugar (48.44 mg g-1 fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA & unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.

      • KCI등재후보

        Relationship of Nitrate Reductase Activity to Leaf Yield, Protein, Sugar and Physiological Attributes in Mulberry (Morus alba L.)

        M. K. Ghosh,B. K. Das,C. Das,A. K. Mishra,P. K. Mukherjee,S. Raje Urs 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.1

        Ten improved mulberry varieties (V1, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year. The nitrate reductase (EC No. 1.6.6.1) activity (NRA, mmol NO2 - h-1g-1 fr. wt.), total soluble protein (mg g-1 fr. wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture%, unit leaf area (cm2), specific leaf weight (g cm-2), total soluble sugar (mg g-1 fr. wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, mmol m-2 s-1) were also studied which showed to vary significantly in tested varieties. Among them, S1635, having higher NRA (13.25 mmol NO2 - h-1g-1 fr. wt.), total soluble protein (39.63 mg g-1 fr. wt.), NPR (16.66 mmol m-2 s-1), total soluble sugar (48.44 mg g-1 fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA & unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.

      • KCI등재후보

        Relationship of Nitrate Reductase Activity to Leaf Yield, Protein, Sugar and Physiological Attributes in Mulberry (Morus alba L.)

        Ghosh, M.K.,Das, B.K.,Das, C.,Mishra, A.K.,Mukherjee, P.K.,Urs, S.Raje Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.8 No.1

        Ten improved mulberry varieties (Vl, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year, The nitrate reductase (EC No. 1.6.6.1) activity (NRA, $\mu$mol N $O_2$- $h^{-1}$ $g^{-1}$ fr, wt.), total soluble protein (mg $g^{-1}$ fr, wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture %, unit leaf area ($\textrm{cm}^2$), specific leaf weight (g c $m^{-2}$ ), total soluble sugar (mg $g^{-1}$ fr, wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, $\mu$㏖ $m^{2}$ $s^{-1}$ ) were also studied which showed to vary significantly in tested varieties. Among them, S1635, haying higher NRA (13.25 $\mu$㏖ N $O_2$- $h^{-l}$ $g^{-1}$ fr, wt.), total soluble protein (39.63mg $g^{-1}$ fr, wt.), NPR(16.66 $\mu$㏖ $m^{-2}$ $s^{-1}$ ), total soluble sugar (48.44 mg $g^{-1}$ fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA af unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.t.

      • SCIESCOPUSKCI등재

        Effect of Sodium Hydroxide plus Hydrogen Peroxide Treated Mustard (Brassica campestris) Straw Based Diets on Rumen Degradation Kinetics (In sacco), Fermentation Pattern and Nutrient Utilization in Sheep

        Mishra, A.S.,Misra, A.K.,Tripathi, M.K.,Santra, A.,Prasad, R.,Jakhmola, R.C. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.3

        Two experiments were conducted to determine the effect of alkaline hydrogen peroxide (AHP) treatment (1% NaOH+1.5% $H_2O_2$; 1 AHPMS, 2% NaOH+1.5% $H_2O_2$; 2AHPMS) on rate and extent of degradation of mustard straw (MS) in sacco in sheep, and its in vivo digestion and ruminal fermentation characteristics when fed to sheep with concentrate (200 g per sheep daily). The treatment of straw with 1 and 2% AHP increased its sodium content by 148 and 296% to that of untreated straw (UMS). There was significant decrease in NDF and hemicellulose contents of AHP treated straw and increase in cellulose and lignin contents. Phenolic acids like ferrulic, $\rho$-coumaric and o-coumaric significantly (p<0.001) reduced by AHP treatment of mustard straw. In first experiment the in sacco degradation of DM, OM and NDF was significantly (p<0.01) greater for 2 AHPMS than for UMS at all incubation periods. The disappearance of nutrient from 1 AHPMS and 2 AHPMS treated straws continue to increase up to 96 h whereas in UMS the peak disappearance was found at 48 h. By using the equation {(y=a+b) ($1-e^{-ct}$)} the degradation rates (c) for DM, OM, and NDF were significantly higher for UMS than AHP treated straws. Level of alkali (1 and 2%) had significant effect on degradation characteristics (a, b, c and $P_{0.05}$) of DM and NDF fraction of MS. However, the effect was not pronounced on OM fraction of MS. In feeding experiment, the intake of nutrients for DM, OM, cell wall constituents and energy was higher on 2 AHPMS, whereas no effect on the digestibility of these nutrients was observed. The apparent nitrogen retention was higher (p<0.05) both in 1 and 2 AHPMS groups. Water intake by animals was significantly increased due to AHP treated mustard straw feeding. Rumen liquor pH was higher in 2 AHPMS fed animals. The $NH_3-N$ of rumen liquor was not affected by feeding of AHP treated MS based diets. Total VFA concentration was significantly (p<0.01) higher in UMS fed group. The fractional out flow rate of DM was higher (p<0.05) in animals fed on 2 AHPMS diets compared to UMS and lAHPMS fed groups. The population of large holotrichs was higher (p<0.05) on AHP treated MS fed diets compared to UMS. The study indicated that treatment of mustard straw with AHP changed its chemical composition towards a better feed. The nutritive value of 2% AHP treated mustard straw was better in terms of dry matter intake and apparent nitrogen retention. The higher in sacco DM, OM and NDF disappearance however, was not confirmed by in vivo data in this study.

      • Cadmium(II) and zinc(II) coordination polymers built with an ethynyl backbone containing an unsymmterical amide ligand: Syntheses, crystal structures, and photoluminescent properties

        Mishra, A.,Kim, H.,Lee, S.C.,Min, J.,Lee, M.H.,Chi, K.W. Elsevier Sequoia [etc.] 2013 Inorganica chimica acta Vol.405 No.-

        Two new crystalline coordination polymers have been synthesized with Zn<SUP>2+</SUP> and Cd<SUP>2+</SUP> ions using an N-(4-(pyridinyl-4-ethynyl)phenyl)-isonicotinamide (HL) donor ligand. The ligand readily reacts with metal nitrate in DMF to afford 1-dimensional (1D) coordination polymers 1 and 2 comprising a repeating unit of [Zn(HL)(NO<SUB>3</SUB>)<SUB>2</SUB>(DMF)].DMF and [Cd(HL)(NO<SUB>3</SUB>)<SUB>2</SUB>(DMF)].DMF, respectively. Both complexes 1 and 2 were characterized by IR spectroscopy, elemental analysis, thermogravimetric analysis (TGA), and X-ray diffraction. Both complexes contained a 1D chain in which crystallography revealed two metal ions were connected by each ligand. The weak π-π stacking interactions and intermolecular hydrogen bonds resulted in the one-dimensional network structures. The photoluminescent properties for both complexes were also investigated.

      • KCI등재후보
      • KCI등재후보

        Electronic Structure of Nano-Sized ZnO: A Compton Profile Study

        R. Kumaravel,M. C. Mishra,B. K. Sharma,V. Vyas,G. Sharma 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.1

        The electronic structure of nano-sized ZnO using Compton scattering technique is reported. The nano-crystalline ZnO of size 10.5 nm is synthesized using the chemical route and characterized by XRD, SEM, TEM probes. Using 59.54 keV gamma-rays, the Compton profile measurements are performed on nano-sized as well as bulk ZnO. The present study reveals the narrower electron momentum density in nano-sized ZnO as compared to bulk sample. The ionic model based free atom Compton profiles for a number of configurations of Zn+xO−x (0.0 ≤ x ≤ 2.0) are also performed to estimate the charge transfer on compound formation. The present study suggests different amount of charge transfer from Zn to O atom in the nano-sized and bulk ZnO.

      • KCI등재후보

        Post Infection Physiobiochemical Alteration at Various Intensities of Leaf spot (Myrothecium roridum) in Mulberry

        Kumar, P.M.Pratheesh,Qadri, S.M.H.,Pal, S.C.,Mishra, A.K.,Urs, S.Raje Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.7 No.2

        Changes in biochemical constituents and physiological alteration were studied in various intensities (1-5%, 6-15%, 16-30%, 31-50% and > 50%) of leaf spot (Myrothecium roridum) on mulberry leaves and compared with healthy leaves. Chlorophyll, total soluble sugar and total protein were decreased (P < 0.01), but total phenol increased due to pathogen infection. Changes in biochemical constituents showed significant correlation with intensity of disease. Chlorophyll ($r^2$= 0.92), and protein (($r^2$= 0.83) possessed negative while phenol (($r^2$= 0.61) possessed positive correlation. Photosynthetic rate, transpiration rate, stomatal conductance, moisture content (%) and physiological water use efficiency (pWUE) were decreased, but stomatal resistance increased in the infected leaves. Physiological parameters also possessed significant (P < 0.01) correlation with disease intensity. Photosynthetic rate (($r^2$= 0.96), transpiration rate ($r^2$=0.88), stomatal conductance (($r^2$= = 0.65), physiological water use efficiency (($r^2$= 0.88) and moisture content (r = 0.85) were negatively but stomatal resistance (($r^2$= 0.75) was positively correlated to disease intensities.

      • SCIESCOPUSKCI등재

        Optimization of Solid State Fermentation of Mustard (Brassica campestris) Straw for Production of Animal Feed by White Rot Fungi (Ganoderma lucidum)

        Misra, A.K.,Mishra, A.S.,Tripathi, M.K.,Prasad, R.,Vaithiyanathan, S.,Jakhmola, R.C. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.2

        The objective of the experiment was to determine the optimum cultural [moisture levels (55, 60 and 70%), days of fermentation (7, 14 and 21), temperature (25 and $35^{\circ}C$) of incubation)] and nutritional parameters (urea addition (0 and 2%) and variable levels of single super phosphate (0.25 and 0.50% SSP)) for bio-processing of the mustard (Brassica campestris) straw (MS) under solid-state fermentation (SSF) system. The performance of SSF was assessed in terms of favorable changes in cell wall constituents, protein content and in vitro DM digestibility of the MS. Sorghum based inoculum (seed culture) of Ganoderma lucidum to treat the MS was prepared. The 50 g DM of MS taken in autoclavable polypropylene bags was mixed with a pre-calculated amount of water and the particular nutrient in the straw to attained the desired levels of water and nutrient concentration in the substrate. A significant progressive increase in biodegradation of DM (p<0.001), NDF (p<0.01) and ADF (p<0.05) was observed with increasing levels of moisture. Among the cell wall constituents the loss of ADF fraction was greatest compared to that of NDF. The loss of DM increased progressively as the fermentation proceeded and maximum DM losses occurred at 28 days after incubation. The protein content of the treated MS samples increased linearly up to the day $21^{th}$ of the incubation and thereafter declined at day $28^{th}$, whereas the improvement in in vitro DM digestibility were apparent only up to the day $14^{th}$ of the incubation under SSF and there after it declined. The acid detergent lignin (ADL) degradation was slower during the first 7 days of SSF and thereafter increased progressively and maximum ADL losses were observed at the day $28^{th}$ of the SSF. The biodegradation of DM and ADL was not affected by the variation in incubation temperature. Addition of urea was found to have inhibitory effect on fungal growth. The effect of both the levels (0.25 and 0.50) of SSP addition in the substrate, on DM, NDF, ADF, cellulose and ADL biodegradation was similar. Similarly, the protein content and the in vitro DM digestibility remain unaffected affected due to variable levels of the SSP inclusion in the substrate. From the results it may be concluded that the incubation of MS with 60 percent moisture for 21 days at $35^{\circ}C$ with 0.25 percent SSP was most suitable for MS treatment with Ganoderma lucidum. Maximum delignification, enrichment in the protein content and improvement in in vitro DM digestibility were achieved by adopting this protocol of bioprocessing of MS.

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