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RISS 인기검색어
- 검색키워드
- 저자 : G. Sharma (검색결과 125 건)
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Rao, S.V. Rama,Raju, M.V.L.N.,Reddy, M.R.,Pavani, P.,Sunder, G. Shyam,Sharma, R.P. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.5
An experiment was conducted to study the requirement of calcium (Ca) and non-phytin phosphorus (NPP) in commercial broilers during starter phase. Seven hundred and twenty day-old Vencob male broiler chicks were randomly distributed into 144 stainless steel battery brooders, 5 birds in each. Four levels each of Ca (6, 7, 8, and 9 g/kg) and NPP (3, 3.5, 4, and 4.5 g/kg diet) were fed in a factorial design in a corn-soya basal diet. Levels of dicalcium phosphate and oyster shell grit were adjusted to obtain the desired levels of Ca and NPP. Each diet was fed ad libidum to chicks in 9 battery brooders from one d to 21 d of age. Body weight gain and feed intake were depressed (p<0.01) by increasing the dietary Ca level (8 and 9 g/kg) at lower levels of NPP (3 and 3.5 g/kg diet). The growth depression observed at lower NPP level was alleviated by reducing the Ca content to 6 g/kg diet. The tibia ash content and tibia breaking strength increased with increase in both Ca (>6 g/kg) and NPP (>3 g/kg) levels. The leg abnormality score decreased (p<0.01) with increase in NPP content in the diet at all levels of Ca tested. The serum Ca and inorganic P levels were increased with increase in the level of the respective mineral in the diet, but the serum concentration of Ca and P were inversely related to the level of NPP and Ca, respectively /kg diet. In general, the excretion of macro minerals (Ca, and P), and micro minerals {zinc (Zn), manganese (Mn), iron (Fe), and copper (Cu)} was significantly lower at lower levels of Ca and NPP tested (6 and 3 g/kg diet, respectively). The mineral excretion increased with increase in dietary Ca and NPP levels, more conspicuously at the disproportionate ratio of these minerals (>2:1, Ca and NPP). Similarly, the retention of Zn, Mn, and Fe in liver was significantly higher (p<0.01) at lower levels of Ca and NPP tested. Results from this study indicate that the commercial broilers do not require more than 3 g NPP and 6g Ca/kg diet during starter phase (up to 21 d of age) for optimum weight gain, feed efficiency and utilization of Ca, P, Zn, Mn, Fe and Cu. However, the requirements of these minerals for optimum bone mineralization were higher than the levels suggested above.
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Iso-material contour representation for process planning of heterogeneous object model
G. K. Sharma,B. Gurumoorthy 한국CDE학회 2020 Journal of computational design and engineering Vol.7 No.4
Additive manufacturing is emerging as the preferred process for making heterogeneous objects. Planning the deposition of material is more complex for heterogeneous objects as the material variation has to be tracked along the path. This paper proposes an iso-material contour representation to generate the process plan for additive manufacturing given a smooth representation of heterogeneous object model. These contours represent the iso-material paths for deposition. As these paths shift along the direction of the gradation of material distribution, the deposition respects the gradient of the designed material distribution unlike iso-oriented paths generated by a raster scan method. Since the paths have the same material composition, material frequent change in the material composition is avoided, which, in turn, avoids the uneven deposition caused by the frequent start and stop of deposition while the material is being changed along the paths generated by the traditional raster scan. Associativity between the contours and the corresponding designed material feature is maintained, and therefore, changes in material composition are automatically propagated to the process plan.
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G.K. Sharma,B. Gurumoorthy 한국CDE학회 2019 Journal of computational design and engineering Vol.6 No.3
This paper proposes a general method for ab-initio modelling and representation of heterogeneous objects that are associated with complex material variation over complex geometry. Heterogeneous objects like composites and naturally occurring objects (bones, rocks and meteorites) possess multiple and often conflicting properties (like high hardness and toughness simultaneously), which are associated with random and irregular material distribution. Modelling such objects is desired for numerical analysis and additive manufacturing to develop bio-implants, high-performance tools etc. However, it is difficult to define and map the arbitrary material distribution within the object as the material distribution can be independent of the shape parameters or form features used to construct its solid model. This paper represents the source of random and irregular material distribution by mixed-dimensional entities with a focus on modelling compositional heterogeneity. The domain of effect of each material ref-erence entity is defined automatically by using Medial Axis Transform (MAT), where the material distri-bution can be intuitively prescribed, starting from the material reference entity and terminating at the medial axis segment bounding the corresponding domain. Within such a domain, the spatial variation of the material is captured by a distance field from the material reference entity, which can be controlled locally and independently. These domains are stored using the neighbourhood relation for efficient oper-ations like altering material distribution across the material reference entity and material evaluation for a given geometric location. Results from an implementation for 2.5D objects are shown and the extension to 3D objects is discussed.
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Comprehensive analysis of plant rapid alkalization factor (RALF) genes
Sharma, A.,Hussain, A.,Mun, B.G.,Imran, Q.M.,Falak, N.,Lee, S.U.,Kim, J.Y.,Hong, J.K.,Loake, G.J.,Ali, A.,Yun, B.W. Elsevier Science B.V., Amsterdam. 2016 Plant Physiology and Biochemistry Vol. No.
<P>Receptor mediated signal carriers play a critical role in the regulation of plant defense and development. Rapid alkalization factor (RALF) proteins potentially comprise important signaling components which may have a key role in plant biology. The RALF gene family contains large number of genes in several plant species, however, only a few RALF genes have been characterized to date. In this study, an extensive database search identified 39, 43, 34 and 18 RALF genes in Arabidopsis, rice, maize and soybean, respectively. These RALF genes were found to be highly conserved across the 4 plant species. A comprehensive analysis including the chromosomal location, gene structure, subcellular location, conserved motifs, protein structure, protein-ligand interaction and promoter analysis was performed. RALF genes from four plant species were divided into 7 groups based on phylogenetic analysis. In silico expression analysis of these genes, using microarray and EST data, revealed that these genes exhibit a variety of expression patterns. Furthermore, RALF genes showed distinct expression patterns of transcript accumulation in vivo following nitrosative and oxidative stresses in Arabidopsis. Predicted interaction between RALF and heme ligand also showed that RALF proteins may contribute towards transporting or scavenging oxygen moieties. This suggests a possible role for RALF genes during changes in cellular redox status. Collectively, our data provides a valuable resource to prime future research in the role of RALF genes in plant growth and development. (C) 2016 Elsevier Masson SAS. All rights reserved.</P>
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Sharma, G.T.,Majumdar, A.C.,Gupta, L.K. Asian Australasian Association of Animal Productio 1999 Animal Bioscience Vol.12 No.7
Circulating lymphocytes collected from control and heat-stressed buffaloes were subjected to in vitro culture with glucocorticoids, epinephrine or serotonin and their effect, if any, on the release of intracellular prolactin (PRL) was studied using ELISA and C-ELISA techniques. It was noted from the study that PRL level was higher in lymphocytes than in plasma of the control and heat-stressed animals, and that the PRL levels increased in the plasma of heat-stressed animals compared to that of non stressed animals with a significant decrease in lymphocytic PRL content by heat stress. Epinephrine and serotonin significantly increased the release of intracellular PRL from the lymphocytes of both in the control and the heat-stressed buffaloes but release of PRL from lymphocyte was not significantly changed by cortisol treatment in both control and heat-stressed buffaloes as compared to epinephrine and serotonin in vitro. When lympocytes were incubated with serotonin, it caused drastic lysis of the lymphocytes but epinephirine and cortisol did not show any lysis. It may be concluded from this study that hormones like epinephrine or serotonin known to increase during stress, release intracellular PRL from lymphocytes, the satellite PRL storage/synthesizing organ of blood, although the mechanism of the release is different.
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Sharma, G. Taru,Teotia, Alok,Majumdar, A.C. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.6
Evaluation of the granulosa cell (GC) monolayers developed from small (<5 mm) and large (>5 mm) follicles on the meiotic competence of caprine oocytes during in vitro maturation was done in this study in comparison to the granulosa cell coculture. Ovaries were collected from the local abattoir and follicular contents were aspirated for the monolayer culture. For IVM the oocytes were collected by puncturing the nonatretic follicles (>4 mm). Results revealed that at the same seeding rate, small follicular granulosa cell monolayer achieved confluence 24-48 h earlier than large follicular granulosa cell monolayer. GC monolayers significantly p (<0.05) improved the rate of meiotic resumption and nuclear maturation (84.76% vs 74.74%) after 27 h of culture in comparison to GC coculture. Statistically there was no significant difference in the maturation rate between the caprine oocytes matured over small or large follicular GC monolayers. It is concluded from the present study that GC monolayers support better nuclear and cytoplasmic maturation of growing caprine oocytes which is evident by better maturation rate over GC monolayer as compared to the oocytes matured with GC coculture. Granulosa cells from small and large follicles can be used for IVM with more or less in the same efficiency after conditioning them with maturation media in 18-24 h before the onset of culture.
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