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        Effects of LED Light Quality Combinations on Growth and Leaf Colour of Tissue Culture-Generated Plantlets in Sedum rubrotinctum

        Si Chuncan,Lin Ying,Luo Sumei,Yu Yanhua,Liu Ruiqing,Naz Misbah,Dai Zhicong 한국원예학회 2024 원예과학기술지 Vol.42 No.1

        Different plants have different requirements with regard to light quality, including combinations of R/B LED light on tissue-culture-generated plants. However, the effects of R/B LED light combinations on the growth and colour changes of leaves in succulent plants remains unknown. In this study, to identify the most beneficial light quality and R/B light ratios for the growth of the succulent plant species Sedum rubrotinctum and its production of attractive colours, the growth parameters, pigment levels and leaf colour phenotypes of tissue-culture-generated S. rubrotinctum plantlets were evaluated when the plantlets were exposed to R, 4R1B, 3R1B, 2R4B, B and W treatments for 90 days. The results showed that light quality obviously influenced the growth and leaf colour of tissue-culture-generated S. rubrotinctum plantlets. R light hastened the elongation of stems, and B light was more beneficial for biomass accumulation and leaf and root growth. However, compared with B light, R light, and W light, the R/B combination resulted in a greater amount of fresh biomass and higher values for the shoot/root FW, stem diameter, crown width, total root length, root surface area, and root volume. The R/B combination also markedly increased the contents of anthocyanins and the R/green chromaticity of the leaves. In addition, a principal component analysis (PCA) revealed the following comprehensive ranking: 4R1B > 2R4B > 3R1B > W > B > R. Therefore, the 4R1B combination is suggested as the best treatment for promoting the growth of tissue-culture-generated S. rubrotinctum plantlets and their production of attractive colours. This study provides a practically significant and optimal solution for growth regulation via light quality for the production of S. rubrotinctum as well as important implications for other succulent plant species in the Crassulaceae family.

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        Characterization of P450 monooxygenase gene family in the cotton aphid, Aphis gossypii Glover

        Zhao Likang,Wang Chuanpeng,Gao Xue-ke,Luo Jun-yu,Zhu Xiang-zhen,Wan Sumei 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2

        Cytochrome P450 monooxygenases play an important role in the biosynthesis and detoxification of endogenous compounds. In this study, we analyzed a number of P450 genes through transcriptome-wide and genome analyses in Aphis gossypii Glover, the most notorious cotton pests. A total of 49 CYP-putative sequences were identified and classified into 13 families and 21 subfamilies. We identified a putative P450 motif (FXXGXXXCXG), and further analyzed other conserved sequences, which indicate that forty-seven of the fortynine genes seem to be functional, but one is likely non-functional and one is predicted pseudogenes. We found that P450-encoding genes are conserved in gene structure and organization (number, position, and phase of introns) in cotton aphids. The size, transcription orientation, and comparison with other insects of the cotton aphid P450 family suggested that it has evolutionary differentiation. The distribution across the genome and phylogenetic clustering analysis indicated past occurrence of gene duplication events. These results may provide valuable clues for understanding the evolution of the aphid genome, studying the functions of P450s in cotton aphid, and further selecting candidate genes for novel insect control methods.

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