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Luan Luong Chu,Ramesh Prasad Pandey,Tae-Su Kim,Van Thuy Thi Pham,Jae Kyung Sohng 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01
Single vessel multi-enzyme UDP-α-D-glucose recycling system was coupled with a forward glucosylation reaction to produce glucose moiety conjugated novel derivatives of different tetracycline antibiotic analogues. Among five tetracycline analogues used for the reaction, four molecules (chlorotetracycline, doxytetracycline, meclotetracycline, and minotetracycline) were accepted by a glycosyltransferase enzyme, YjiC, from Bacillus licheniformis to produce glucoside derivatives. However, the enzyme was unable to conjugate sugar unit to rolitetracycline. All glucosides of tetracycline derivatives were characterized by ultraviolet absorbance maxima, ultra-pressure liquid chromatography coupled with photo-diode-array, and high-resolution quadruple time-of-flight electrospray mass spectrometry analyses. These synthesized glucosides are novel tetracycline derivatives. The conversion of tetracycline analogues to respective glucosides has been found to be very low yet. This gives the clue that the enzyme has low preferences toward tetracycline molecules. Attachment of bulky group in rolitetracycline might have prevented the molecule to reach the catalytic cleft of GT enzyme. Results of this research could become a basis to explore the possibility of synthesizing glucosylated derivatives of different tetracyclines using GT enzyme.
Bacterial endophytes from ginseng and their biotechnological application
Luan Luong Chu,Hanhong Bae 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.1
Ginseng has been well-known as a medicinal plant for thousands of years. Bacterial endophytes ubiquitously colonize the inside tissues of ginseng without any disease symptoms. The identification of bacterial endophytes is conducted through either the internal transcribed spacer region combined with ribosomal sequences or metagenomics. Bacterial endophyte communities differ in their diversity and composition profile, depending on the geographical location, cultivation condition, and tissue, age, and species of ginseng. Bacterial endophytes have a significant effect on the growth of ginseng through indole-3-acetic acid (IAA) and siderophore production, phosphate solubilization, and nitrogen fixation. Moreover, bacterial endophytes can protect ginseng by acting as biocontrol agents. Interestingly, bacterial endophytes isolated from Panax species have the potential to produce ginsenosides and bioactive metabolites, which can be used in the production of food and medicine. The ability of bacterial endophytes to transform major ginsenosides into minor ginsenosides using b-glucosidase is gaining increasing attention as a promising biotechnology. Recently, metabolic engineering has accelerated the possibilities for potential applications of bacterial endophytes in producing beneficial secondary metabolites.
( Ramesh Prasad Pandey ),( Luan Luong Chu ),( Tae-su Kim ),( Jae Kyung Sohng ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.2
The single-vessel multienzyme UDP-α-D-glucose recycling system was coupled with a forward glucosylation reaction to produce novel glucose moiety-conjugated derivatives of different tetracycline antibiotic analogs. Among five tetracycline analogs used for the reaction, four molecules (chlorotetracycline, doxytetracycline, meclotetracycline, and minotetracycline) were accepted by a glycosyltransferase enzyme, YjiC, from Bacillus licheniformis to produce glucoside derivatives. However, the enzyme was unable to conjugate sugar units to rolitetracycline. All glucosides of tetracycline derivatives were characterized by ultraviolet absorbance maxima, ultra-pressure liquid chromatography coupled with photodiode array, and high-resolution quadruple time-of-flight electrospray mass spectrometry analyses. These synthesized glucosides are novel tetracycline derivatives.
Synthesis of enzymatically modified isoquercitrin
Jae Youn Bae,Chu Luong Luan,Jae Kyung Sohng 한국당과학회 2017 한국당과학회 학술대회 Vol.2017 No.01
Quercetin is suggested to be potentially beneficial for the prevention of various diseases. Enzymatically modified isoquercitrin was prepared by glycosylation of quercetin and the subsequent of oligoglucosylation of quercetin 3-O-β-glucoside (isoquercitrin) produced from quercetin. To enhance the production of isoquercitrin with high specificity, yield, and purity, an enzymatic bioconversion method was developed enzyme UGT78K1 from glycine max, which has quercetin transforming ability. UGT78K1 could effectively transform the quercetin into isoquercitrin. Enzymatically modified isoquercitrin was glucosylated to its oligoglucosyl quercetin 3-O-β-Glucoside by levansucrase of a genetically modified strain of Leuconostoc mesenteroides. levansucrase industrially important glycansucrase from L. mesenteroides.
Biosynthesis of a novel fisetin glycoside from engineered Escherichia coli
라메쉬,Prakash Parajuli,Luan Luong Chu,김승영,송재경 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.43 No.-
Escherichia coli BL21(DE3)/DpgiDzwfDgalU mutant was engineered by overexpressing thymidinediphosphate (dTDP)-D-glucose synthase (tgs), dTDP-D-glucose 4,6-dehydratase (dh), and a sugaraminotransferase (wecE) from different sources to produce a pool of dTDP-4-amino-4,6-dideoxy-Dgalactosein the cell cytosol. To this recombinant mutant, two Arabidopsis thaliana glycosyltransferases(ArGT-3 and ArGT-4) were overexpressed to generate two glycosylation platforms (E. coli BL21(DE3)/DpgiDzwfDgalUTDW-3 and E. coli BL21(DE3)/DpgiDzwfDgalUTDW-4), which were accessed for theglycosylation offisetin. As a result, one of the two systems, E. coli BL21(DE3)/DpgiDzwfDgalUTDW-3, wasable to conjugate 4-amino-4,6-dideoxy-D-galactose sugar at the 3-OH position offisetin, producing anunnaturalfisetin 3-O-4-amino-4,6-dideoxy-D-galactoside.
이미나,Hee Taek Kim,Seo Young Jo,Luan Luong Chu,Kei-Anne Baritugo,Mary Grace Baylon,JIN WON LEE,Jeong-Geol Na,Lyul Ho Kim,Tae-Wan Kim,CHULHWAN PARK,Soon Ho Hong,Jeong Chan Joo,Si Jae Park 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.1
The production of industrial chemicals from renewable biomass resources is a promising solution to overcome the society’s dependence on petroleum and to mitigate the pollution resulting from petroleum processing. Klebsiella pneumoniae is a nutritionally versatile bacterium with numerous native pathways for the production of wellknown and industrially important platform chemicals derived from various sugars. Genomic sequence analyses have shown that the K. pneumoniae genome has a high similarity with that of Escherichia coli, the most studied organism, which is used in industrial biotechnology processes for fuel and chemical production. Hence, K. pneumoniae can be considered as a promising platform microorganism that can be metabolically engineered for the high-level production of bio-based chemicals. This review highlights the substrate metabolism and the metabolic engineering strategies developed in K. pneumoniae for the production of biobased chemicals.