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        Effects of Saturated Long-chain Fatty Acid on mRNA Expression of Genes Associated with Milk Fat and Protein Biosynthesis in Bovine Mammary Epithelial Cells

        Qi, Lizhi,Yan, Sumei,Sheng, Ran,Zhao, Yanli,Guo, Xiaoyu Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.3

        This study was conducted to determine the effects of saturated long-chain fatty acids (LCFA) on cell proliferation and triacylglycerol (TAG) content, as well as mRNA expression of ${\alpha}s1$-casein (CSN1S1) and genes associated with lipid and protein synthesis in bovine mammary epithelial cells (BMECs). Primary cells were isolated from the mammary glands of Holstein dairy cows, and were passaged twice. Then cells were cultured with different levels of palmitate or stearate (0, 200, 300, 400, 500, and 600 ${\mu}M$) for 48 h and fetal bovine serum in the culture solution was replaced with fatty acid-free BSA (1 g/L). The results showed that cell proliferation tended to be increased quadratically with increasing addition of stearate. Treatments with palmitate or stearate induced an increase in TAG contents at 0 to 600 ${\mu}M$ in a concentration-dependent manner, and the addition of 600 ${\mu}M$ was less effective in improving TAG accumulation. The expression of acetyl-coenzyme A carboxylase alpha, fatty acid synthase and fatty acid-binding protein 3 was inhibited when palmitate or stearate were added in culture medium, whereas cluster of differentiation 36 and CSN1S1 mRNA abundance was increased in a concentration-dependent manner. The mRNA expressions of peroxisome proliferator-activated receptor gamma, mammalian target of rapamycin and signal transducer and activator of transcription 5 with palmitate or stearate had no significant differences relative to the control. These results implied that certain concentrations of saturated LCFA could stimulate cell proliferation and the accumulation of TAG, whereas a reduction may occur with the addition of an overdose of saturated LCFA. Saturated LCFA could up-regulate CSN1S1 mRNA abundance, but further studies are necessary to elucidate the mechanism for regulating milk fat and protein synthesis.

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        Microarray Analysis of Genes Involved with Shell Strength in Layer Shell Gland at the Early Stage of Active Calcification

        Liu, Zhangguo,Zheng, Qi,Zhang, Xueyu,Lu, Lizhi Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.5

        The objective of this study was to get a comprehensive understanding of how genes in chicken shell gland modulate eggshell strength at the early stage of active calcification. Four 32-week old of purebred Xianju hens with consistent high or low shell breakage strength were grouped into two pairs. Using Affymetrix Chicken Array, a whole-transcriptome analysis was performed on hen's shell gland at 9 h post oviposition. Gene ontology enrichment analysis for differentially expressed (DE) transcripts was performed using the web-based GOEAST, and the validation of DE-transcripts was tested by qRT-PCR. 1,195 DE-transcripts, corresponding to 941 unique genes were identified in hens with strong eggshell compared to weak shell hens. According to gene ontology annotations, there are 77 DE-transcripts encoding ion transporters and secreted extracellular matrix proteins, and at least 26 DE-transcripts related to carbohydrate metabolism or post-translation glycosylation modification; furthermore, there are 88 signaling DE-transcripts. GO term enrichment analysis suggests that some DE-transcripts mediate reproductive hormones or neurotransmitters to affect eggshell quality through a complex suite of biophysical processes. These results reveal some candidate genes involved with eggshell strength at the early stage of active calcification which may facilitate our understanding of regulating mechanisms of eggshell quality.

      • KCI등재

        Infuence of Dynamic Characteristics of Induction Motor on Voltage Sag Caused by Short Circuit Fault

        Jiming Chen,Hai Zuo,Qianyu Yu,Lizhi Qi,Mingxiao Zhu 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.4

        Voltage sag is a signifcant problem that afects the quality of power, and some researches have been performed on this theme. The characteristic of load is an important factor afecting the voltage sag, which is rarely studied. This paper investigates the infuence of dynamic characteristics of induction motor on voltage sag caused by short circuit fault. The analytic expression of induction electromotive force is derived by space vector method. The mechanism of amplitude and waveform changes caused by dynamic load characteristics of induction motor are analyzed. The results indicate that the voltage at the load terminal of the induction motor is higher than that at the static load during the sag. Besides, the voltage sag waveform is not in rectangular shape. The static load terminal voltage returns to normal value quickly, while the voltage at the load terminal of the induction motor needs about 200 ms after the fault is removed. Induction motors can reduce the magnitude of voltage sags caused by short circuit faults, but will extend the duration. At the same time, the bigger the induction motor capacity is, the longer the voltage recovery time after sag is.

      • Color Adjustment Based on Support Vector Regression for Multi-View Video

        Huadong Sun,Xuesong Jin,Zhipeng Fan,Lizhi Zhang,Qian Wu 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.1

        Significant color discrepancies between the different camera views can be observed in multi-view video sequences. In this paper, a color adjustment algorithm based on support vector regression is proposed. A mapping function is established by extracted feature points from original-image and target-image. Then the mapping function is applied to the original image to obtain the corrected image. Experimental results show that the proposed method can produce good correction result. It also shows that the color differences between multi-view video can be effectively reduced by SVR.

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