Identification of genomic-wide genetic links between cutaneous melanoma and obesity-related physical traits via cFDR

Lin Shen,Shen Runnan,Huang Jingqian,Liu Yanhan,Li Hongpeng,Xu Qingfang 한국유전학회 2023 Genes & Genomics Vol.45 No.12

Background Both epidemiological and clinical studies have suggested the comorbidity between cutaneous melanoma (CM) and obesity-related physical traits. However, it remains unclear about their shared genetic architecture. Objective To determine the shared genetic architecture between CM and obesity-related physical traits through conditional false discovery rate (cFDR) analysis. Method Quantile–quantile plots were firstly built to assess the pleiotropic enrichment of shared single nucleotide polymorphisms between CM and each trait. Then, cFDR and conjunctional cFDR (ccFDR) were used to identify the shared risk loci between CM and each trait. Moreover, the functional evaluation of shared risk genes was carried out through analyses of expression quantitative trait loci (eQTL), Kyoto Encyclopedia of Genes and Genomes and gene ontology, respectively. Finally, single-cell sequence analysis was performed to locate the expression of eQTL-mapped genes in tissues. Results Successive pleiotropic enrichment was found between CM and 5 obesity-related traits or height. 24 shared risk loci were identified between CM and 13 traits except appendicular lean mass using ccFDR analysis, with 17 novel and 4 validated loci. The functions of ccFDR-identified and eQTL-mapped genes were revealed to be mainly involved in cellular senescence, proliferation, meiotic nuclear division, cell cycle, and the metabolism of lipid, cholesterol and glucose. Single-cell sequence analysis showed that keratinocytes contribute to the occurrence and aggressiveness of CM through secreting paracrine cytokines. Conclusion Our findings demonstrate the significant genetic correlation between CM and obesity-related physical traits, which may provide a novel genetical basis for the pathogenesis and treatment of CM.

The influence of vehicles on the flutter stability of a long-span suspension bridge

Yan Han,Shuqian Liu,C.S. Cai,Jianren Zhang,Suren Chen,Xuhui He 한국풍공학회 2015 Wind and Structures, An International Journal (WAS Vol.20 No.2

The presence of traffic on a slender long-span bridge deck will modify the cross-section profileof the bridge, which may influence the flutter derivatives and in turn, the critical flutter wind velocity of thebridge. Studies on the influence of vehicles on the flutter derivatives and the critical flutter wind velocity ofbridges are rather rare as compared to the investigations on the coupled buffeting vibration of thewind-vehicle-bridge system. A typical streamlined cross-section for long-span bridges is adopted for bothexperimental and analytical studies. The scaled bridge section model with vehicle models distributed on thebridge deck considering different traffic flow scenarios has been tested in the wind tunnel. The flutterderivatives of the modified bridge cross section have been identified using forced vibration method and theresults suggest that the influence of vehicles on the flutter derivatives of the typical streamlined cross-sectioncannot be ignored. Based on the identified flutter derivatives, the influence of vehicles on the flutter stabilityof the bridge is investigated. The results show that the effect of vehicles on the flutter wind velocity isobvious.

The Protective Role of TLR3 and TLR9 Ligands in Human Pharyngeal Epithelial Cells Infected with Influenza A Virus

Yan Han,Ming-yu Xu,Nan Sun,Dan-hong Liu,Zhi-jian Bo 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.3

In this study we aim to extensively investigate the anti-influenza virus immune responses in humanpharyngeal epithelial cell line (Hep-2) and evaluate the protective role of Toll-like receptor (TLR)ligands in seasonal influenza A H1N1 (sH1N1) infections in vitro. We first investigated the expressionof the TLRs and cytokines genes in resting and sH1N1 infected Hep-2 cells. Clear expressions of TLR3,TLR9, interleukin (IL)-6, tumour necrosis factor (TNF)-α and interferon (IFN)-β were detected inresting Hep-2 cells. After sH1N1 infection, a ten-fold of TLR3 and TLR9 were elicited. Concomitantwith the TLRs activation, transcriptional expression of IL-6, TNF-α and IFN-β were significantlyinduced in sH1N1-infected cells. Pre-treatment of cells with poly I:C (an analog of viral double-strandedRNA) and CpG-ODN (a CpG-motif containing oligodeoxydinucleotide) resulted in a strong reductionof viral and cytokines mRNA expression. The results presented indicated the innate immune responseactivation in Hep-2 cells and affirm the antiviral role of Poly I:C and CpG-ODN in the protectionagainst seasonal influenza A viruses.

Flutter stability of a long-span suspension bridge during erection

Yan Han,Shuqian Liu,C.S. Cai,Chunguang Li 한국풍공학회 2015 Wind and Structures, An International Journal (WAS Vol.21 No.1

The flutter stability of long-span suspension bridges during erection can be more problematic and more susceptible to be influenced by many factors than in the final state. As described in this paper, numerical flutter stability analyses were performed for the construction process of Zhongdu Bridge over Yangtze River using the commercial FE package ANSYS. The effect of the initial wind attack angle, the sequence of deck erection, the stiffness reduction of stiffening girders, the structural damping, and the cross cables are discussed in detail. It was found that the non-symmetrical sequence of deck erection was confirmed to be aerodynamically favourable for the deck erection of long-span suspension bridges and the best erection sequence should be investigated in the design phase. While the initial wind attack angle of -3o is advantageous for the aerodynamic stability, +3o is disadvantageous compared with the initial wind attack angle of 0o during the deck erection. The stiffness reduction of the stiffening girders has a slight effect on the flutter wind speed of the suspension bridge during erection, but structural damping has a great impact on it, especially for the early erection stages.

Test for Influence of Socket Connection Structure on Dynamic Response of Prefabricated Pier under Vehicle Collision

Yan Han,Zhihao Liu,Longlong Wang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.3

In order to obtain the difference of anti-collision performance between the socket assembled bridge pier and integral bridge pier, this research conducted the model test of the bridge piers subjected to vehicle impact. The influence of the socket connection form and structure parameters such as embedment depth, interface roughness, and reserved hole opening size on the response of bridge piers to vehicle collision was analyzed by comparing them with those of the cast-in-place bridge pier. Results show that vehicle speed and socket depth are important factors affecting the dynamic response of socket bridge piers to vehicle crash. The maximum impact force on a bridge pier is obviously affected by the embedment depth of the pier column, but no clear change rule exists between them. The opening size of the reserved cavity affects the impact force slightly when the vehicle speed is low, but gradually increases with the increase in vehicle speed. The damage degree of the bridge pier gradually decreases with the increase in the embedment depth of the pier column and increases with the increase in vehicle speed. The acceleration of the bridge pier tends to increase with the vehicle speed, but no clear law of change with the socket depth exists. The maximum strain of the steel bar at the root of the socket pier is greater than that of the impact position, and the maximum strain distribution of the steel bars of the pier column with the socket embedment depth greater than or equal to 1.0 times diameter of the column is similar to that of the cast-in-place one. The maximum strain of steel bars of the both are at the root of the pier column. The study results provide a data reference for further studying the actual vehicle bumping on socket bridge pier and prefabricated bridge anti-collision design.