Effects of Nitrate Addition on Rumen Fermentation, Bacterial Biodiversity and Abundance

Liping Zhao,Qingxiang Meng,Liping Ren,Wei Liu,Xinzhuang Zhang,Yunlong Huo,Zhenming Zhou 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.10

This study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with Ion Torrent sequencing and real-time polymerase chain reaction. Three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. Nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p<0.01), butyrate and isovalerate concentration numerically (p = 0.07). The alpha (p>0.05) and beta biodiversityof ruminal bacteria were not affected by nitrate. Nitrate increased typical efficient cellulolytic bacteria species (Ruminococcus flavefaciens, Ruminococcus ablus, and Fibrobacter succinogenes) (p<0.01; p = 0.06; p = 0.02). Ruminobactr, Sphaerochaeta, CF231, and BF311 genus were increased by 1% nitrate. Campylobacter fetus, Selenomonas ruminantium, and Mannheimia succiniciproducens were core nitrate reducing bacteria in steers and their abundance increased linearly along with nitrate addition level (p<0.01; p = 0.02; p = 0.04). Potential nitrate reducers in the rumen, Campylobacter genus and Cyanobacteria phyla were significantly increased by nitrate (p<0.01; p = 0.01).To the best of our knowledge, this was the first detailed view of changes in ruminal microbiota by nitrate. This finding would provide useful information on nitrate utilization and nitrate reducer exploration in the rumen.

ANALYSIS OF PILE GROUP BASED ON THE NON-LINEAR HORIZONTAL FOUNDATION COEFFICIENT

LIPING ZHAO,SHILIN HAN,LIAN HAN 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

Ionic Liquid Induced Supramolecular Self-Assembly of Poly(3,4-ethylenedioxythiophene)/ Poly(4-styrenesulfonate) Thin Films with Enhanced Conductivity and Tunable Nanoporosity

Liping Zhao,Jishan Qiu,Xiaojun Cao,Wenyong Dong,Jichun You,Yongjin LI 한국고분자학회 2013 Macromolecular Research Vol.21 No.4

We present a facile strategy, for the first time as the best of our known, to prepare high conducting poly(3,4-ethylenedioxythiophene)/poly(4-styrenesulfonate) (PEDOT:PSS) film with a novel nanoporous morphology directly from a commercial PEDOT:PSS dispersion. Both conducting properties and surface morphology of PEDOT:PSS film can be systematically tunable by simply controlling addition of an ionic liquid, 1,3-dihydroxy-2-methylimidazolium bis(trifluoromethylsulfonyl)-imide (DHIL). The electrical conductivity increases from 0.07 for pristine PEDOT:PSS to 55 S cm-1 after addition of 2 wt% DHIL, with no necessary of any further heat treatment, which is around 800 times increase in the electrical conductivity. On the other hand, the discrete compact PEDOT:PSS film is gradually transformed into the nanoporous film with addition of DHIL. It is considered that such size-tunable porous PEDOT:PSS films with high surface area as well as high conductivity combining solution-processibility show great potential in applications that require high interfacial area, such as flexible electronic components, nextgeneration catalytic, and separation supports.

Effects of Nitrate Addition on Rumen Fermentation, Bacterial Biodiversity and Abundance

Zhao, Liping,Meng, Qingxiang,Ren, Liping,Liu, Wei,Zhang, Xinzhuang,Huo, Yunlong,Zhou, Zhenming Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.10

This study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with Ion Torrent sequencing and real-time polymerase chain reaction. Three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. Nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p<0.01), butyrate and isovalerate concentration numerically (p = 0.07). The alpha (p>0.05) and beta biodiversityof ruminal bacteria were not affected by nitrate. Nitrate increased typical efficient cellulolytic bacteria species (Ruminococcus flavefaciens, Ruminococcus ablus, and Fibrobacter succinogenes) (p<0.01; p = 0.06; p = 0.02). Ruminobactr, Sphaerochaeta, CF231, and BF311 genus were increased by 1% nitrate. Campylobacter fetus, Selenomonas ruminantium, and Mannheimia succiniciproducens were core nitrate reducing bacteria in steers and their abundance increased linearly along with nitrate addition level (p<0.01; p = 0.02; p = 0.04). Potential nitrate reducers in the rumen, Campylobacter genus and Cyanobacteria phyla were significantly increased by nitrate (p<0.01; p = 0.01).To the best of our knowledge, this was the first detailed view of changes in ruminal microbiota by nitrate. This finding would provide useful information on nitrate utilization and nitrate reducer exploration in the rumen.

The effect of multiwalled carbon nanotube doping on the CO gas sensitivity of SnO₂-based nanomaterials

Zhao, Liping,Choi, Mansoo,Kim, Hyun-Su,Hong, Seong-Hyeon IOP Pub 2007 Nanotechnology Vol.18 No.44

<P>A simple <I>in situ</I> method is applied for the synthesis of SnO<SUB>2</SUB>-coated multiwalled nanotubes (MWNTs), in which MWNT bundles are uniformly coated with a monolayer of SnO<SUB>2</SUB> nanoparticles (∼4 nm). A hybrid SnO<SUB>2</SUB> material combined with 0.1% wt MWNTs is fabricated and its CO gas-sensing properties are evaluated. SnO<SUB>2</SUB>-coated MWNTs are homogeneously distributed in the SnO<SUB>2</SUB> matrix and the specific surface area of the hybrid material is nearly two times higher compared to pure SnO<SUB>2</SUB> material. The electrical resistance is found to be dramatically decreased by three orders of magnitude compared to a conventional SnO<SUB>2</SUB> sensor. The CO gas sensitivity is found to be three times higher than that of a SnO<SUB>2</SUB> sensor, with good stability, indicating that the addition of 0.1% wt MWNTs is effective in improving the sensitivity towards CO. A model is proposed to explain the improvement of CO gas sensitivity for our hybrid MWNT/SnO<SUB>2</SUB> material.</P>

Mathematical programming model of process plant safety layout using the equipment vulnerability index

Liping Guo,Zhirong Wang,Pinkun Guo,Jinghong Wang,Dan Zhao 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.4

Safety is the focus of attention in plant layout problems. Previous studies have often expressed safety as a cost of risk, that is, the cost of property losses that may occur in an accident. In this paper, the influence of uncertainty on the equipment vulnerability is quantitatively considered and a more reliable process plant layout is proposed. The equipment vulnerability index is used to evaluate the vulnerability level of the target equipment in case of an accident, which is applied to propose a mixed-integer nonlinear optimized process plant layout to minimize domino risk. In addition, a decision matrix is applied to determine whether the risk level of the optimized layout of the target equipment is acceptable. Damage probability and vulnerability are the basic inputs of this matrix. The proposed method was applied to a coal-water slurry gasification process and the results show that the layout obtained by the proposed model has better practical value than the current layout, reducing the domino risk by 53.2%. Meanwhile, the model can be used to identify critical equipment and select targeted safety measures during the production stage.

Extreme Value Prediction of Traffic Loads Using the Average Conditional Exceedance Rate Method

Liping Zhang,Jianqing Bu,Liming Zhou,Wenlong Cao,Cunbao Zhao,Wei Chai 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.12

An efficient prediction of the extreme value of traffic loads is crucial for the structural design, reliability evaluation, maintenance planning, and further life-cycle cost analysis of bridges. In this work, a novel method is proposed for predicting the appropriate extreme traffic load distribution. Specifically, the average conditional exceedance rate (ACER) statistical model is estimated from the historical traffic loads which was collected through a weigh-in-motion system installed in toll stations. The basic idea of the ACER approach lies in the introduction of a cascade of conditioning approximations and the average exceedance rate to capture the dependence effects and obtain the data tail, the trend features of which are fitted with a similar Gumbel distribution function and extrapolated to the concerned level. An illustration case dealing with traffic loads using the ACER strategy is presented, the extreme value and confidence interval (CI) in any return period can be predicted by application of this approach. Furthermore, the peaks-over-threshold (POT) method based on the asymptotic extreme theory is also applied to illustrate the advantages of the ACER method. The ACER method has advantages in analyzing extreme traffic loads, with good robustness and the ability to handle extreme value prediction for different sampling strategies, it also can produce more accurate confidence intervals and predicts consistent extreme values. The study results are expected to help accurately determine traffic loads and ensure safety in bridge engineering.

KLF9 promotes autophagy and apoptosis in T-cell acute lymphoblastic leukemia cells by inhibiting AKT/mTOR signaling pathway

Zhao Jie,He Shaolong,Xiang Chenhuan,Zhang Shaoli,Chen Xinyue,Lu Xinyi,Yao Qiong,Yang Liping,Ma Liangming,Tian Weiwei 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3

Background T -cell acute lymphoblastic leukemia (T-ALL) is considered a malignant tumor with a high mortality rate. To combat this disease, exploring the mechanism of T-ALL progression is urgently needed. Krüppel-like factors (KLFs) are known as the transcription factors and mediate series of biological processes. KLF9 is a member of the KLF family which could serve as a tumor suppressor gene in most solid tumors. GEO Database analysis showed that KLF9 expression in normal T cells was higher than T-ALL cell lines and patients. However, the possible role of KLF9 in T-ALL progression is still unclear. Objective To uncover the possible eff ects of Krüppel-like transcription factor 9 (KLF9) on the progression of T-Acute lymphoblastic leukemia (T-ALL). Results The expression of KLF9 was low in human T-ALL cells. KLF9 suppressed the viability of T-ALL cells. In addition, KLF9 stimulated the apoptosis as well as autophagy of T-ALL cells. Mechanically, KLF9 suppressed AKT/mTOR pathway in T-ALL cells. Conclusion KLF9 suppressed viability and promoted autophagy as well as apoptosis in T-ALL cells by inhibiting AKT/ mTOR pathway.

Mechanism of the anti-liver fibrosis effect of Periplaneta americana extracts that promote apoptosis of HSC-T6 cells through the Bcl-2/Bax signaling pathway

Yuan Liping,Yang Xiao,He Ying,Zhao Yanwen,Chen Yi,Yang Yongshou,Xiao Peiyun 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Periplaneta americana L. (Blattidae) extract (PA-B) possesses anti-oxidant, anti-inflammatory, and hep atoprotective properties; however, its mechanism of action in liver fibrosis remains unclear. Herein, we inves tigated the hepatoprotective effect and mechanism of action of PA-B. HSC-T6 cells treated with PA-B were stimulated with TGF-β1, and immunofluorescence, western blotting, Hoechst 33,342 staining, confocal laser scanning microscopy, and flow cytometry were performed. Furthermore, the effects of PA-B were verified in a rat model of hepatic fibrosis established using 40 % carbon tetrachloride (CCl 4 ). PA-B induced apoptosis of HSC-T6 cells by inhibiting proliferation (maximum inhibition ratio 99.08 %), reducing mitochondrial membrane po tential, and ameliorating apoptosis-related proteins (P < 0.01). PA-B (120 mg/kg) significantly alleviated CCl 4 -induced hepatic histopathological fibrosis and reduced the levels of aspartate aminotransferase (from 27.68 to 20.58 U/L) and alanine aminotransferase (from 16.88 to 5.93 U/L; P < 0.05 or P < 0.01). Immunohistochemistry and western blotting showed that α-SMA levels were reduced (from 0.49 to 0.23) and that the Bcl/Bax signaling pathway was significantly inhibited by PA-B (120 mg/kg; P < 0.05 or P < 0.01). PA-B exhibits anti-fibrotic effects by mediating the Bcl-2/Bax pathway and promoting HSC-T6 cell apoptosis, providing a basis for its development as a drug to treat liver fibrosis.

Potent antibacterial and antibiofilm activities of TICbf-14, a peptide with increased stability against trypsin

Wang Liping,Liu Xiaoyun,Ye Xinyue,Zhou Chenyu,Zhao Wenxuan,Zhou Changlin,Ma Lingman 한국미생물학회 2022 The journal of microbiology Vol.60 No.1

The poor stability of peptides against trypsin largely limits their development as potential antibacterial agents. Here, to obtain a peptide with increased trypsin stability and potent antibacterial activity, TICbf-14 derived from the cationic peptide Cbf-14 was designed by the addition of disulfide-bridged hendecapeptide (CWTKSIPPKPC) loop. Subsequently, the trypsin stability and antimicrobial and antibiofilm activities of this peptide were evaluated. The possible mechanisms underlying its mode of action were also clarified. The results showed that TICbf-14 exhibited elevated trypsin inhibitory activity and effectively mitigated lung histopathological damage in bacteria-infected mice by reducing the bacterial counts, further inhibiting the systemic dissemination of bacteria and host inflammation. Additionally, TICbf-14 significantly repressed bacterial swimming motility and notably inhibited biofilm formation. Considering the mode of action, we observed that TICbf-14 exhibited a potent membrane-disruptive mechanism, which was attributable to its destructive effect on ionic bridges between divalent cations and LPS of the bacterial membrane. Overall, TICbf-14, a bifunctional peptide with both antimicrobial and trypsin inhibitory activity, is highly likely to become an ideal candidate for drug development against bacteria.