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The objective of this study was to analyze the target genes and regulatory function of miR-34a in Megalobrama amblycephala using second-generation highthroughput sequencing and bioinformatic tools. Functional enrichment analysis was performed by gene ontology. MiR- 34a and target gene expression levels were measured in M. amblycephala fed normal and high-carbohydrate diets. The results revealed that miR-34a was highly conserved in several species, and miR-34a of M. amblycephala has a close evolutionary relationship to that of zebrafish and common carp. miRanda, TargetScan, RNAhybrid predicted 5,185, 6,282 and 2,168 target genes, respectively, and 645 target genes were in common. According to annotation information, the target genes were enriched in phosphate metabolism, glycerophospholipid metabolism, Golgi vesicle transport, cell division, and other biological processes (P < 0.05). Pathway enrichment analysis revealed that these target genes were mainly enriched in alpha-linolenic acid and linoleic acid metabolism, ether lipid metabolism, VEGF signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, and MAPK signaling pathway (P < 0.05). The regulatory role of miR-34a was more significant in the liver than in the brain of M. amblycephala. MiR-34a regulates glucose lipid homeostasis induced by high glucose diets by upregulating hepatic PI3K/Akt, FOXO, and TOR signaling pathways.
Rubber forming is an important forming process for the manufacture of aircraft sheet metal parts. The springback is one of the main defects in rubber forming. Classical springback compensation by displacement adjustment method using finite simulation is not satisfactory. In this research, the algorithms of compensating the arc and flange surface of complex curved flange with correction formula are proposed by experiment. The correction formula was developed based on the CATIA V5 R19 using Component Application Architecture. Compensate profile is presented including surface pick up, line pick up, division, compensation, extending, and trimming. The die profile of part with complex curved flanges in aircraft could be designed rapidly. It was found that the forming pressure has a little effect on the springback. This is within the tolerance limits of the part. The results reveal the method can achieve the industrial part precisely. The method is demonstrated on an aircraft wing rib part.
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버지니아 울프의 ‘델레웨이 부인’은 작가의 이야기 전달 방식인 의식의 흐름 기법으로 쓰여진 전형적인 소설임과 동시에 여성에 관한 울프의 시각을 반영하는 페미니즘 소설이다. 울프는 이 작품 에서 당시 영국 지배계층의 우둔함을 묘사함으로써 그들의 생활 모습을 생생하게 드러내고 있다. 그리고 그 지배계층 속에는 남성에 대한 극단적인 모습이 그려지는데 즉,그 어느 것 보다도 우선시 되는 왕권에 대한 맹목적인 찬양과 충성심으로 대변되는 남성에 대한 극단적인 모습으로 가득 채워져 있다. 그러한 당시 사회 분위기는 다른 사람의 삶을 방관한 체 편안한 삶을 쫓아 살다 결국,삶을 포기하는 사람들을 만들어 내는가 하면 반면에 자살까지 감행할 정도로 우울증에 시달리는 일종의 희생자라고 할 수 있는 사람들을 양산하게 되었다. 울프는 이러한 문제를 알고 있었지만 여성에 대한 적절한 해결책을 찾을 수는 없었다. 1세기 후,전 세계적으로 페미니즘에 대한 의식이 발전되어 왔지만,울프가 제기 한 100년 전의 문제점에 대한 적절한 해결책이 마련되었는가? 이 논문에서는 위에 언급한 이러한 문제들에 대해 논의하고자 한다. Feminism, a very simple word, but has profound meanings and when you go into depth of it, you will sense a complex and mixture feeling of it. Everyone seems has his or her own opinion and view on it. As the society is coming forward, the feminism is developing too. The publishing of Sexual Politics written by Kate Millet is generally thought to be the symbol of Feminism Literary Theory. But, actually, in 1929, Virginia Woolf's a room of one's own has already brought forward many serious problems about women and literary through its exaggerating and irony style and since the feminism literary theory took shape.(1) Now, we can find so many branches about feminism, not only in the field of literary, including Cultural Feminism, Erotic Feminism, Eco-Feminism, Individualist, or Libertarian Feminism, Liberal Feminism, and so on. Here, this article just will explain the view on feminism through Mrs. Dalloway. First this article is to introduce Virginia Woolf and her background of writing Mrs. Dalloway.
A LiV3O8/polyaniline (PAn) composite was prepared by the in-situ polymerization method assisted by sodium dodecyl sulfate and ammonium persulfate. The as-prepared powders were investigated by XRD, SEM, and galvanostatic discharge/charge analysis. It was found that the introduction of PAn to LiV3O8 can effectively buffer the mechanical stress and restrain the number of phase changes of composite material during the electrochemical cycling. Compared with pristine LiV3O8, LiV3O8/PAn composite maintains a reversible capacity of 212.1 mAh g−1 at the current density of 30 mA g−1 after 50 cycles, approximately 22.6%, much higher than the former.
Er-doped LiV3O8 as cathode material for secondary lithium batteries was prepared through a rheological phase reaction method. The as-prepared materials were investigated by x-ray diffraction (XRD), scanning electron microscopy (SEM), and galvanostatic discharge/charge measurements. The results indicate that Er doped phase preserves the layered structure of the pristine LiV3O8 and has an enlarged interlayer spacing. Compared to LiV3O8 sample, Er-doped LiV3O8 sample displays more uniform particles and large surface area. The electrochemical test shows that Er doping does not change the process of Li+ insertion/deinsertion. Er-doped LiV3O8 electrode exhibits an initial discharge capacity of 294.2 mAh g−1 and maintains a stable capacity of 220.7 mAh g−1 after 50 cycles, indicating a greatly improved good cycleability comparing with the undoped one.
Co3(PO4)2-coated LiV3O8 has been successfully synthesized and used as positive material for rechargeable lithium batteries by a facile liquid phase method. The as-prepared powders were characterized by x-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), and the galvanostatic discharge/charge experiments. As-prepared Co3(PO4)2-coated LiV3O8 forms a good layered structure with a poor cyrstallinity. SEM reveals that Co3(PO4)2-coated LiV3O8 has uniform particle distribution and reduced particle size when compared with bare one. The Co3(PO4)2 coating layer is about 33 - 59 nm forming a continuous lumps attached to LiV3O8 particle surface. Co3(PO4)2-coated LiV3O8 electrode shows increased capacity and more stable cycling. The first and 35th discharge capacities of the Co3(PO4)2-coated LiV3O8electrode are 322.8 mAh g−1 and 235.7 mAh g−1 in the range of 4.0 - 1.8 V at a current rate of 30 mA g−1, respectively. The improved electrochemical performance is assigned to the greatly reduced LiV3O8 particle with uniform morphology. Co3(PO4)2-coating further benefits the phase transitions of LiV3O8 during discharge/charge while preventing parasite reactions between electrode surface and electrolyte.
An investigation of the mechanism of period-doubling bifurcation in a voltage mode controlled buck-boost converter operating in discontinuous conduction mode is conducted from the viewpoint of nonlinear dynamical systems. The discrete iterative model describing the dynamics of the close-loop is derived. Period-doubling bifurcation occurs at certain values of the feedback factor. Results from numerical simulations and experiments are provided to verify the evolution of perioddoubling bifurcation, and the results are consistent with the theoretical analysis. These results show that the buck-boost converters exhibit a wide range of nonlinear behavior, and the system exhibits a typical period-doubling bifurcation route to chaos under particular operating conditions.