Comparison of the Gene Expression Profiles Between Smokers With and Without Lung Cancer Using RNA-Seq

Cheng, Peng,Cheng, You,Li, Yan,Zhao, Zhenguo,Gao, Hui,Li, Dong,Li, Hua,Zhang, Tao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Lung cancer seriously threatens human health, so it is important to investigate gene expression changes in affected individuals in comparison with healthy people. Here we compared the gene expression profiles between smokers with and without lung cancer. We found that the majority of the expressed genes (threshold was set as 0.1 RPKM) were the same in the two samples, with a small portion of the remainder being unique to smokers with and without lung cancer. Expression distribution patterns showed that most of the genes in smokers with and without lung cancer are expressed at low or moderate levels. We also found that the expression levels of the genes in smokers with lung cancer were lower than in smokers without lung cancer in general. Then we detected 27 differentially expressed genes in smokers with versus without lung cancer, and these differentially expressed genes were foudn to be involved in diverse processes. Our study provided detail expression profiles and expression changes between smokers with and without lung cancer.

Bicluster and Pathway Enrichment Analysis of HCV-induced Cirrhosis and Hepatocellular Carcinoma

Cheng, Peng,Cheng, You,Su, Mei X.,Li, Dong,Zhao, Guo Z.,Gao, Hui,Li, Yan,Zhu, Jie Y.,Li, Hua,Zhang, Tao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide and the most common form of liver cancer. However, while it is associated frequently with hepatitis C virus (HCV) there is only an elementary understanding of its molecular pathogenesis. Methods: To gain insight into the molecular mechanisms of HCV-induced hepatocarcinogenesis, we performed microarray analysis on 75 surgical liver samples from 48 HCV-infected patients. Results: There were 395 differentially expressed geness between cirrhotic samples and HCC samples. Of these, 125 genes were up-regulated and 270 genes were down-regulated. We performed pathway enrichment analysis and screened as described previously. Conclusions: The differentially expressed genes might be involved in hepatocarcinogenesis through upregulating the pathways of ECM-receptor interaction, focal adhesion, cell adhesion molecules and other cancer-related pathways, and downregulating the pathways of "complement and coagulation cascades". We hope our results could aid in seeking of therapeutic targets for HCV-induced hepatocellular carcinoma.

Numerical analysis on tensile properties of composite hybrid bonded/bolted joints with flanging

Xiaoquan Cheng,Jie Zhang,Jikui Zhang,Peng Liu,Yujia Cheng,Yahong Xu 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.26 No.3

A detailed study was carried out on the tensile properties of the single-lap joint of a steel panel bolted/bonded to a composite laminate with a flanging. Finite element model (FEM) was established to predict the strength and to analyze the damage propagation of the hybrid joints by ABAQUS/Standard, which especially adopted cohesive elements to simulate the interface between the laminate and adhesive. The strength and failure mode predicted by FEM were in good agreement with the experimental results. In addition, three influence factors including adhesive thickness, bolt preload and bolt-hole clearance were studied. The results show that the three parameters have effect on the first drop load of the load-displacement curve, but the effect of bolt-hole clearance is the largest. The bolt-hole clearance should be avoided for hybrid joints.

애니메이션 〈나타지마동강세〉 ‘나타’의 반영웅적 특징 연구

장봉정(Zhang Peng-cheng),김일태(Kim Il-tae) 한국만화애니메이션학회 2021 만화애니메이션연구 Vol.- No.64

지난 10년 동안 중국 애니메이션 산업의 발전과 상업화가 심화되고 있다. 중국 애니메이션의 수준도 향상되어 흥행과 평판이 좋은 애니메이션 작품들이 많이 나왔다. 2019년 여름 중국에서 개봉해 총 7억2천6백만 달러라는 글로벌 흥행을 거둔 〈나타지마동강세〉(哪吒之魔童降世)는 중국 영화사 흥행 1위 애니메이션이다. 〈나타지마동강세〉는 주인공 ‘나타’의 전통적인 영웅의 이미지를 변화시켜 반영웅적 ‘나타’의 이미지를 만들었고 클래식한 ‘나타’의 모습을 창의적으로 새롭게 만들었다. 중국 신화 속 소년 영웅 ‘나타’는 스크린에서 관객들의 공감과 사랑을 받았다. 본 연구는 〈나타지마동강세〉의 주인공 ‘나타’의 반영웅적 특징을 통해 ‘동기-행동-결과’의 모델로 전통 애니메이션 〈나타요해〉의 주인공 ‘나타’의 영웅적 특징을 비교 분석하였다. 〈나타지마동강세〉의 ‘나타’는 다양한 캐릭터의 특징을 가지고 있으며, 영웅적인 인물의 특징과 함께 ‘악역’ 인물의 특징을 동시에 가지고 있다. 본 연구는 〈나타지마동강세〉의 주인공 ‘나타’의 반영웅적 특징을 생애, 이미지, 성격, 저항정신 등 네가지로 분석하였다. 또한 〈나타요해〉 속 ‘나타’의 이미지, 성격 등을 비교 분석하였다. 반영웅 ‘나타’는 기구한 생애, 마화된(魔化) 이미지, 과장된 성격, 강한 저항정신을 가지고 있다. 연구를 통해 〈나타요해〉의 영웅 ‘나타’보다 〈나타지마동강세〉의 반영웅 ‘나타’가 더 다양한 캐릭터 특징과 모순된 캐릭터의 심리, 그리고 흡입력 있는 스토리로 관객이 쉽게 공감할 수 있다는 결과를 도출할 수 있었다. 따라서 본 논문은 〈나타지마동강세〉의 주인공인 ‘나타’의 반영웅적 특징을 연구해 반영웅적 인물의 공통적인 특징을 소개하고 반영웅적 특징 분석 방법을 제시하는 한편 ‘나타’의 반영웅적 특징의 구성요소를 분석하였다. 그리고 본 논문의 연구 결과가 중국 애니메이션의 이론적 연구를 위한 자료로 축적되고 활용될 것으로 기대한다. The development and commercialization of the Chinese animation industry have been intensifying over the past decade. The level of Chinese animation has also improved, resulting in many popular and reputable animation works. 〈Nezha〉, which was released in China in the summer of 2019 and was a global hit of $726 million, is the No. 1 animation in Chinese film history. 〈Nezha〉 changed the image of the traditional hero of the main character “Nezha” to create an antiheroic “Nezha” image and creatively renewed the image of the classical “Nezha” This recreated Chinese mythical young hero, “Nezha” was sympathized and loved by the audience on the screen in 2019. This study compared and analyzed the heroic characteristics of “Nezha”, the main character of 〈Nezha〉, as the model of “Motivation-Activity-Result” and the main character of the traditional animation 〈Prince Nezha’s Triumph Against Dragon King〉. “Nezha” in 〈Nezha〉 has the characteristics of complex characters, and has the characteristics of both heroic characters and villainous characters. Therefore, this study analyzed the antisocial characteristics of “Nezha” the main character of 〈Nezha〉 including life, image, personality, and spirit of resistance. In addition, we compared and analyzed the image and personality of “Nezha” in 〈Prince Nezha’s Triumph Against Dragon King〉. The antihero “Nezha” has a bizarre life, an image of machisma, an exaggerated personality, and a strong spirit of resistance. The audience was able to easily sympathize with the character’s psychology, which contradicted the character’s characteristics, and the engaging story, rather than the hero “Nezha” of 〈Prince Nezha’s Triumph Against Dragon King〉. This paper studied the anti-heroic characteristics of “Nezha” the main character of 〈Nezha〉 introducing the common characteristics of antiheroic characters and presenting methods for analyzing anti-heroic features, while analyzing the components of “Nezha”. Through the study of this study, it is expected that data for theoretical research of Chinese animation will be accumulated.

Mechanical Heterogeneity of Heat-Resistant EV31A Magnesium Alloy with Large Size Processed by Direct-Chill Casting

Deping Zhang,Boqiong Li,Chaojie Che,Baosheng Liu,Peng Cheng,Yali Zhao,Jinhui Zhang,Liren Cheng 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.10

High quality of large-sized EV31A benchmark alloy was fabricated by direct-chill casting, without significant composition segregation along its radial direction. As-cast samples show noticeable mechanical heterogeneity, such as strength decreasing but ductility increasing from the surface to the center positions of the ingot. After heat treatment, however, mechanical properties of the alloy are nearly the same in each position, indicating mechanical homogeneity, which is mainly attributed to the strengthening mechanism transition from grain-boundary hardening in as-cast samples to precipitation hardening in peak-aged samples. In addition, we found that solute hardening is the athermal strengthening which contributes more to high-temperature strength than precipitation hardening, as evidenced by lower strength loss from 25 to 250 °C in T4-treated sample than in peak-aged sample. Solute hardening effect is thermally stable and extends to high temperatures, which favors the retention of linear strain hardening by retarding dynamic recovery. This work thus provides new insight into designing heat-resistant Mg alloys and understanding mechanical heterogeneity of large-scale Mg alloy ingots.

Enhancing Astaxanthin Accumulation in Haematococcus pluvialis by Coupled Light Intensity and Nitrogen Starvation in Column Photobioreactors

( Wen-wen Zhang ),( Xue-fei Zhou ),( Ya-lei Zhang ),( Peng-fei Cheng ),( Rui Ma ),( Wen-long Cheng ),( Hua-qiang Chu ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12

Natural astaxanthin mainly derives from a microalgae producer, Haematococcus pluvialis. The induction of nitrogen starvation and high light intensity is particularly significant for boosting astaxanthin production. However, the different responses to light intensity and nitrogen starvation needed to be analyzed for biomass growth and astaxanthin accumulation. The results showed that the highest level of astaxanthin production was achieved in nitrogen starvation, and was 1.64 times higher than the control group at 11 days. With regard to the optimization of light intensity utilization, it was at 200 μmo/m2/s under nitrogen starvation that the highest astaxanthin productivity per light intensity was achieved. In addition, both high light intensity and a nitrogen source had significant effects on multiple indicators. For example, high light intensity had a greater significant effect than a nitrogen source on biomass dry weight, astaxanthin yield and astaxanthin productivity; in contrast, nitrogen starvation was more beneficial for enhancing astaxanthin content per dry weight biomass. The data indicate that high light intensity synergizes with nitrogen starvation to stimulate the biosynthesis of astaxanthin.

Breastfeeding and Ovarian Cancer Risk: a Systematic Review and Meta-analysis of 40 Epidemiological Studies

Li, Da-Peng,Du, Chen,Zhang, Zuo-Ming,Li, Guang-Xiao,Yu, Zhi-Fu,Wang, Xin,Li, Peng-Fei,Cheng, Cheng,Liu, Yu-Peng,Zhao, Ya-Shuang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

The present systematic review and meta-analysis was conducted to assess any association between breastfeeding and the risk of ovarian cancer. A systematic search of published studies was performed in PUBMED and EMBASE and by reviewing reference lists from retrieved articles through March 2013. Data extraction was conducted independently by two authors. Pooled relative risk ratios were calculated using random-effect models. Totals of 5 cohort studies and 35 case-control studies including 17,139 women with ovarian cancer showed a 30% reduced risk of ovarian cancer when comparing the women who had breastfed with those who had never breastfed (pooled RR = 0.70, 95% CI: 0.64-0.76; p = 0.00), with significant heterogeneity in the studies (p = 0.00; I2 = 76.29%). A significant decreasd in risk of epithelial ovarian cancer was also observed (pooled RR = 0.68, 95% CI: 0.61-0.76). When the participants were restricted to only parous women, there was a slightly attenuated but still significant risk reduction of ovarian cancer (pooled RR = 0.76, 95% CI: 0.69-0.83). For total breastfeeding duration, the pooled RRs in the < 6 months, 6-12 months and > 12 months of breastfeeding subgroups were 0.85 (95% CI: 0.77-0.93), 0.73 (95% CI: 0.65-0.82) and 0.64 (95%CI: 0.56-0.73), respectively. Meta-regression of total breastfeeding duration indicated an increasing linear trend of risk reduction of ovarian cancer with the increasing total breastfeeding duration (p = 0.00). Breastfeeding was inversely associated with the risk of ovarian cancer, especially long-term breastfeeding duration that demonstrated a stronger protective effect.

Impact of Latrogenic Biliary Injury during Laparoscopic Cholecystectomy on Surgeon’s Mental Distress A Nationwide Survey from China

( Tian Yang ),( Hai-su Dai ),( Lei Liang ),( Cheng-cheng Zhang ),( Zhang-jun Cheng ),( Yong-hai Peng ),( Yao-ming Zhang ),( Zhi-yu Chen ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Iatrogenic biliary injury (IBI) following laparoscopic cholecystectomy (LC) is the most common and recognized iatrogenic complications. Little is known whether LC-IBI would lead to surgeon’s mental distress. This study reports the incidence of surgeon’s mental distress who have caused LC-IBI and risk factors of surgeon’s severe mental distress (SMD). Methods: A cross-sectional survey in the form of electronic questionnaire was conducted among Chinese general surgeons who have caused LC-IBI. The six collected clinical features relating to mental distress included: 1) feeling burnout, anxiety, or depression, 2) avoiding performing LC, 3) having physical reactions when recalling the incidence, 4) having the urge to quit surgery, 5) taking psychiatric medications, and 6) seeking professional psychological counseling. Univariable and multivariable analyses were performed to identify risk factors of SMD, which was defined as meeting ≥3 of the above-mentioned clinical features. Results: Among 1,466 surveyed surgeons, 1,236(84.3%) experienced mental distress following LC-IBI, and nearly half (49.7%, 614/1236) had SMD. Multivariable analyses demonstrated that surgeons from non-university affiliated hospitals (OR:1.873), patients who required multiple repair operations (OR:4.075), patients who required hepaticojejunostomy/partial hepatectomy (OR:1.859), existing lawsuit litigation (OR:10.491), existing violent doctor-patient conflicts (OR:4.995), needing surgeons’ personal compensation (OR:2.531), and additional administrative punishment by hospitals (OR:2.324) were independent risk factors of surgeon’s SMD. Conclusions: Four out of five surgeons experienced mental distress following LC-IBI, and nearly half had SMD. Several independent risk factors of SMD were identified, which could help to make strategies to improve mental well-being of these surgeons.

Study of Neurotoxic Effects and Underlying Mechanisms of Aconitine on Cerebral Cortex Neuron Cells

Cheng Peng,Tao Zheng,Fan Yang,Yun-Xia Li,Ding-Kun Zhang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.11

The study investigated the neurotoxic effects and underlying mechanisms of aconitine on cerebral cortex neuron cells prepared from neonatal SD rats. The uniform design and MTT method were applied to study the effect of aconitine with different concentrations at scheduled time. The influence of aconitine at the maximal toxicity concentration was observed using optical microscope and electron microscope. The influences of aconitine on neuron cells membrane, neuron cells’ inner balance, energy metabolism and neurotransmitters were observed to investigate the action mechanisms of aconitine. The results indicated that the maximal toxicity-concentration was 2% and the critical time were 30 s, 1 min and 20 min respectively. The effects of aconitine on neuron cells’ morphology included cells synapse’s fracture, cells membrane fragment, mitochondria’s swell, cytoplasmic vacuoles, nuclear chromatin’s condensation and accumulation. The stability of biomembrane, the internal milieu and the energy metabolism were also disturbed with the increase of activity of LDH and concentration of neurotransmitters (acetylcholine, opioid, catecholamine and SP) in culture medium, the increase of the activity of ACP and [Na+], [Ca2+] concentration, and the decrease of Na+-K+-ATP, [K+], [Mg2+] and glycogen concentration in the cells. Toxic mechanisms of aconitine damaging neuron cells may be because it inhibited the activity of Na+-K+-ATP, influenced the concentrations of [Na+], [K+], [Ca2+], [Mg2+] and neurotransmitters in the cells, which resulted in the injuries of cells’ morphology and function.

Calcination and sintering effects on the microstructure and dielectric properties of CaCu3Ti4O12 ceramics

Peng Liu,Lei Cao,Jian-Ping Zhou,Ya-Juan Wang,Li-Na Su,Cheng Liu,Huai-Wu Zhang 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.4

CaCu3Ti4O12 (CCTO) ceramics were fabricated by a conventional solid-state reaction at various calcination and sintering temperatures. Depending on the relative dielectric constant (εr) at 1 kHz, the specimens can be categorized into three types:type A (εr < 5 × 104), type B (5 × 104 < εr < 105) and type C (εr > 105). The value of εr is closely related to the microstructure:type-A specimens show a morphology with large grains embedded in a small-grained matrix, type-B specimens present a network structure and type-C specimens display a core-shell structure. Different dielectric loss variation tendencies are observed in the type-A specimens, which can be well explained by impedance spectroscopy analysis and further confirmed by the EDX results. Also, the proper calcination and sintering temperature ranges needed to achieve good comprehensive properties are given.