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        Tryptophan Supplements Promote Pregnancy Success in Mice Challenged with Pseudorabies Virus (PRV) by Regulating the Expression of Systemic Cytokines, Immunoglobulins, PRV-Specific Protein Profiles, and Toll-Like Receptors

        Shixiu Qiu,Zhengfeng Fang,De Wu,Yan Lin,Lianqiang Che 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.7

        Tryptophan (Trp) plays an important role in regulating the maternal immune response, a key determinant of the success or failure of pregnancy, but whether Trp supplements can prevent a pseudorabies virus (PRV)-induced failure of pregnancy remains unknown. This study examined the effect of three dietary Trp levels (0.25%, 0.35%, and 0.5%) on the immunity and reproduction of PRV-challenged pregnant mice. PRV challenge resulted in decreased live embryo numbers, live litter sizes, and serum progesterone and interleukin (IL)-10 concentrations, but increased the levels of serum immunoglobulins (Igs) (PRV-specific antibody [IgG, IgA, and IgM]) and IL-1β. Live embryo numbers, live litter sizes, serum progesterone concentration, and IgG and PRV-specific antibody levels on day 9 of pregnancy were all increased dose-dependently by Trp inclusion in the diet of PRV-challenged mice. Increased Trp levels in PRV-challenged mice promoted the up-regulation of uterine and embryonic indoleamine 2,3-dioxygenase expression, but attenuated the up-regulation of uterine and embryonic Toll-like receptor (TLR) 3 and TLR9 expression and increased serum interferon-γ concentration. Collectively, Trp supplements might improve reproductive performance of PRV-challenged pregnant mice by down-regulating TLR expression and pro-inflammatory cytokine synthesis, by up-regulating PRV-specific antibody and immunoglobulin synthesis, and by elevating the concentrations of anti-inflammatory cytokines and progesterone.

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        Substitution of soybean meal with detoxified Jatropha curcas kernel meal: Effects on performance, nutrient utilization, and meat edibility of growing pigs

        Li, Yang,Chen, Ling,Zhang, Yuhui,Wu, Jianmei,Lin, Yan,Fang, Zhengfeng,Che, Lianqiang,Xu, Shengyu,Wu, De Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.6

        Objective: The study was conducted to investigate the effects of replacing soybean meal (SBM) with different levels of detoxified Jatropha curcas kernel meal (DJM) in growing pig diets on growth performance, nutrients digestibility and meat edibility. Methods: A total of 144 pigs with initial body weight of $20.47{\pm}1.44kg$, were randomly allocated to 6 dietary treatments with 6 replications per treatment and 4 pigs per replication for a period of 79 days. Six diets (DJM0, DJM15, DJM30, DJM45, DJM60, and DJM75) were formulated using DJM to replace 0%, 15%, 30%, 45%, 60%, and 75% of SBM. From d 37 to 42, feces and urine were total collected from six barrows in each treatment. At day 79, thirty-six pigs were slaughtered for sampling. The feed intake and weight gain were recorded, while the intestinal morphology, digestive enzyme activities, nutrient digestibility and the content of residual phorbol esters in muscles were determined. Results: The results showed that increasing the replacement of SBM with DJM decreased the parameters including body weight, average daily gain, average daily feed intake, gain-to-feed ratio, weight and villus heights of duodenum, villus height and villus height/crypt depth of jejunum, digestive enzymes (protease, amylase, lipase, and trypsin) activities, and nutrients digestibility (nitrogen deposition, digestibility of nitrogen, energy digestibility, and total nitrogen utilization) (linear, p<0.05; quadratic, p<0.05) and there was no significant difference among DJM0, DJM15, and DJM30 in all measured indices. The highest diarrhea morbidity was observed in DJM75 (p<0.05). Phorbol esters were not detected in pig muscle tissues. Conclusion: The DJM was a good protein source for pigs, and could be used to replace SBM up to 30% (diet phorbol esters concentration at 5.5 mg/kg) in growing pig diets with no detrimental impacts on growth performance, nutrient utilization, and meat edibility.

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