MiR-454 Prompts Cell Proliferation of Human Colorectal Cancer Cells by Repressing CYLD Expression

Liang, Hong-Liang,Hu, Ai-Ping,Li, Sen-Lin,Xie, Jia-Ping,Ma, Qing-Zhu,Liu, Ji-Yong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Previous studies have shown that miR-454 plays an important role in a variety of biological processes in various human cancer cells. However, the underlying mechanisms of this microRNA in colorectal cancer (CRC) cells remain largely unknown. In the present study, we investigated the miR-454 role in CRC cell proliferation. We found that miR-454 expression is markedly upregulated in CRC tissues and CRC cells compared with the matched tumor adjacent tissues and the FHC normal colonic cell line. Ectopic expression of miR-454 promoted the proliferation and anchorage-independent growth of CRC cells, whereas inhibition of miR-454 reduced this effect. Bioinformatics analysis further revealed cylindromatosis (CYLD), a putative tumor suppressor as a potential target of miR-454. Data from luciferase reporter assays showed that miR-454 directly binds to the 3'-untranslated region (3'-UTR) of CYLD mRNA and repressed expression at both transcriptional and translational levels. In functional assays, CYLD-silenced in miR-454-in-transfected SW480 cells have positive effect to promote cell proliferation, suggesting that direct CYLD downregulation is required for miR-454-induced CRC cell proliferation. In sum, our data provide compelling evidence that miR-454 functions as an onco-miRNA, playing a crucial role in the promoting cell proliferation in CRC, and its oncogenic effect is mediated chiefly through direct suppression of CYLD expression.

Quantification of Multifunctional Dipeptide YA from Oyster Hydrolysate for Quality Control and Efficacy Evaluation

Xie, Cheng-Liang,Kang, Sang Soo,Lu, Ciyong,Choi, Yeung Joon Hindawi 2018 BioMed research international Vol.2018 No.-

<P>YA is an angiotensin-I-converting enzyme- (ACE-) inhibitory peptide from oyster hydrolysate with antihypertensive activity. Its antioxidant and anti-inflammatory activity were investigated in this study. YA can dose-dependently quench DPPH and ABTS radical and inhibit lipopolysaccharide-induced nitric oxide in RAW 264.7 cells. YA is a multifunctional peptide and was selected as an indicator for quality control and efficacy evaluation of oyster hydrolysate. A practical HPLC/UV assay for YA quantification was developed and validated. It was proved to be accurate and reliable, according to parameters such as specificity, linearity, precision, and accuracy. The quantity results of YA showed that the stage of enzymatic hydrolysis was a critical control point for quality control; the efficacy of oyster hydrolysate can be enhanced after digested in the gastrointestinal tract due to the release of YA by brush border peptidases. Therefore, YA from oyster hydrolysate is a potential bioactive ingredient for functional foods to combat hypertension.</P>

Effects of Strain Energy Adjustment: A Case Study of Rock Failure Modes during Deep Tunnel Excavation with Different Methods

Liang-Tao Xie,Peng Yan,Wenbo Lu,Ming Chen,Gaohui Wang 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.10

The strain energy adjustment processes and rock failure modes corresponding to different excavation methods, such as Tunnel Boring Machine (TBM) or blasting, are quite different during construction of deep tunnel. Based on the diversion tunnel excavation of Jin-Ping II hydropower station (JPII) in southwestern China, the distribution characteristics of damage zones and adjustment process of rock strain energy under different excavation methods are analyzed and discussed, and the occurrences of rock bursts in the diversion tunnels are also monitored and analyzed. Research reveals that, the adjustment process of rock strain energy and the distribution of damage zones are obviously different under different excavation methods, and the depth and distribution of damage zone are positively correlated with the accumulation depth of rock strain energy. For blasting excavation, due to the combined effects of blast loading and in situ stress transient unloading, the surrounding rock is damaged seriously. The accumulation depth of rock strain energy is significantly larger than that by TBM excavation, while the accumulation peak of rock strain energy is smaller. For TBM excavation, the strain energy releases smoothly and slowly, and much more strain energy is accumulated in the vicinity of excavation face. Under similar geological conditions in the JPII, the rock bursts of intensive and mediate grades can be more frequently observed after blasting for the impact of severe excavation disturbance, and the strain energy transient adjustment may be the main disturbance contributor. While during TBM excavation, due to the smooth adjustment process of rock strain energy, the disturbance to surrounding rock is limited, and the accumulation peak of rock strain energy is higher and closer to the excavation face, which may result in more spalling events or minor rock bursts.

Isoflavone-enriched soybean (<i>Glycine</i> max) leaves prevents ovariectomy-induced obesity by enhancing fatty acid oxidation

Xie, Cheng-liang,Kang, Sang Soo,Cho, Kye Man,Park, Ki Hun,Lee, Dong Hoon Elsevier 2018 Journal of Functional Foods Vol.43 No.-

<P><B>Abstract</B></P> <P>Dietary isoflavones have been gaining increased interest in the field of functional foods due to their ability to ameliorate several postmenopausal symptoms. Using ovariectomized (OVX) rats supplemented with isoflavone-enriched soybean leaves (IESLs), we investigated the effect of dietary isoflavones on menopausal-associated obesity. Oral administration of IESLs significantly reduced OVX-induced weight gain and visceral fat mass. Additionally, IESLs significantly ameliorated OVX-induced hepatic steatosis as reflected by decreased triacylglycerol accumulation in the liver and decreased serum alanine transaminase and aspartate aminotransferase levels. These anti-obesity effects of IESLs were confirmed to be due to enhanced fatty acid oxidation via up-regulation of peroxisome proliferator activated receptor alpha, peroxisomal acyl-coenzyme A oxidase 1, and carnitine palmitoyltransferase 1a mRNA expression. These results demonstrate that IESLs may be an effective functional food-based approach to protect against menopause-associated obesity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ethylene-treated soybean (<I>Glycine</I> max) leaves were rich of isoflavones. </LI> <LI> Isoflavone-enriched soybean leaves (IESLs) reduced ovariectomy-induced weight gain and visceral fat mass. </LI> <LI> IESLs ameliorated ovariectomy-induced hepatic steatosis. </LI> <LI> IESLs enhanced fatty acid oxidation by up-regulating mRNA expression of PPARα, ACOX1 and CPT1a. </LI> </UL> </P>

Organic Acid Extraction of Fluoride from Antarctic Krill Euphausia superba

Xie, Cheng Liang,Kim, Han Soo,Shim, Kil Bo,Kim, Yeon Kye,Yoon, Na Yeong,Kim, Poong Ho,Yoon, Ho Dong The Korean Society of Fisheries and Aquatic Scienc 2012 Fisheries and Aquatic Sciences Vol.15 No.3

The amount of fluoride removed from Antarctic krill via organic acid extraction depends on the extraction time, concentration, extraction volume, and the nature of the acid itself. The fluoride content in Euphausia superba was determined by measuring the concentration of fluoride ion in acidic extracts using an ion-selective electrode. The best results were obtained by adding 50 mL of 0.01 M citric acid to 0.3-0.5 g of krill and extracting for 5 min. Under these conditions, recoveries of fluoride from frozen whole krill and krill meat were 95.6-99.5% and 97.5-101.3%, respectively. The dry basis fluoride contents of krill by-product, krill meat and the boiled krill were 705, 298, and 575 ppm, respectively. These levels were significantly reduced by citric acid extraction.

Angiotensin I-Converting Enzyme Inhibitor Derived from Cross-Linked Oyster Protein

Xie, Cheng-Liang,Kim, Jin-Soo,Ha, Jong-Myung,Choung, Se-Young,Choi, Yeung-Joon Hindawi Publishing Corporation 2014 BioMed research international Vol.2014 No.-

<P>Following cross-linking by microbial transglutaminase, modified oyster proteins were hydrolyzed to improve inhibitory activity against angiotensin-converting enzyme (ACE) inhibitory activity with the use of a single protease, or a combination of six proteases. The oyster hydrolysate with the lowest 50% ACE inhibitory concentration (IC<SUB>50</SUB>) of 0.40 mg/mL was obtained by two-step hydrolysis of the cross-linked oyster protein using Protamex and Neutrase. Five ACE inhibitory peptides were purified from the oyster hydrolysate using a multistep chromatographic procedure comprised of ion-exchange, size exclusion, and reversed-phase liquid chromatography. Their sequences were identified as TAY, VK, KY, FYN, and YA, using automated Edman degradation and mass spectrometry. These peptides were synthesized, and their IC<SUB>50</SUB> values were measured to be 16.7, 29.0, 51.5, 68.2, and 93.9 <I>μ</I>M, respectively. Toxicity of the peptides on the HepG2 cell line was not detected. The oyster hydrolysate also significantly decreased the systolic blood pressure of spontaneously hypertensive rats (SHR). The antihypertensive effect of the oyster hydrolysate on SHR was rapid and long-lasting, compared to commercially obtained sardine hydrolysate. These results suggest that the oyster hydrolysate could be a source of effective nutraceuticals against hypertension.</P>

IRE1α protects against osteoarthritis by regulating progranulin-dependent XBP1 splicing and collagen homeostasis

Liang Li,Zhang Fengmei,Feng Naibo,Kuang Biao,Fan Mengtian,Chen Cheng,Pan Yiming,Zhou Pengfei,Geng Nana,Li Xingyue,Xian Menglin,Deng Lin,Li Xiaoli,Kuang Liang,Luo Fengtao,Tan Qiaoyan,Xie Yangli,Guo Fen 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Osteoarthritis (OA) is a full-joint, multifactorial, degenerative and inflammatory disease that seriously affects the quality of life of patients due to its disabling and pain-causing properties. ER stress has been reported to be closely related to the progression of OA. The inositol-requiring enzyme 1α/X-box-binding protein-1 spliced (IRE1α/XBP1s) pathway, which is highly expressed in the chondrocytes of OA patients, promotes the degradation and refolding of abnormal proteins during ER stress and maintains the stability of the ER environment of chondrocytes, but its function and the underlying mechanisms of how it contributes to the progression of OA remain unclear. This study investigates the role of IRE1α/ERN1 in OA. Specific deficiency of ERN1 in chondrocytes spontaneously resulted in OA-like cartilage destruction and accelerated OA progression in a surgically induced arthritis model. Local delivery of AdERN1 relieved degradation of the cartilage matrix and prevented OA development in an ACLT-mediated model. Mechanistically, progranulin (PGRN), an intracellular chaperone, binds to IRE1α, promoting its phosphorylation and splicing of XBP1u to generate XBP1s. XBP1s protects articular cartilage through TNF-α/ERK1/2 signaling and further maintains collagen homeostasis by regulating type II collagen expression. The chondroprotective effect of IRE1α/ERN1 is dependent on PGRN and XBP1s splicing. ERN1 deficiency accelerated cartilage degeneration in OA by reducing PGRN expression and XBP1s splicing, subsequently decreasing collagen II expression and triggering collagen structural abnormalities and an imbalance in collagen homeostasis. This study provides new insights into OA pathogenesis and the UPR and suggests that IRE1α/ERN1 may serve as a potential target for the treatment of joint degenerative diseases, including OA.

Anti-obesity Effects of Fermented Soy-powder Milk with Lactobacillus Plantarum P1201 in High-fat Diet-induced Obese Mice

Cheng-liang Xie,Chung Eun Hwang,Cheol Kyu Oh,Nal Ae Yoon,Jin Hyun Ryu,Joo Yeon Jeong,Gu Seob Roh,Hyun Joon Kim,Gyeong Jae Cho,Wan Sung Choi,Sang Soo Kang,Kye Man Cho,Dong Hoon Lee 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Preliminary study of genome-wide association identified novel susceptibility genes for thyroid-related hormones in Chinese population

Huang Liang,Bai Fenghua,Zhang Yutian,Zhang Shanshan,Jin Tianbo,Wei Xingwei,Zhou Xiaoli,Lin Mei,Xie Yufei,He Chanyi,Lin Qi,Xie Tian,Ding Yipeng 한국유전학회 2022 Genes & Genomics Vol.44 No.8

Background: Thyroid hormones are critical regulators of metabolism, development and growth in mammals. However, the genetic association of thyroid-related hormones in the Chinese Han population is not fully understood. Objective: We aimed to identify the genetic loci associated with circulating thyroid-related hormones concentrations in the healthy Chinese Han population. Methods: Genotyping was performed in 124 individuals using Applied Biosystems™ Axiom™ PMDA, and 796,288 single nucleotide polymorphisms (SNPs) were available for the GWAS analysis. For replication, eleven SNPs were selected as candidate loci for genotyping by Agena MassARRAY platform in additional samples (313 subjects). The values of p < 5 × 10- 6 suggest a suggestively significant genome-wide association with circulating thyroid-related hormones concentrations. Results: We identified that rs11178277 (PTPRB, p = 4.88 × 10- 07) and rs7320337 (LMO7DN-KCTD12, p = 1.22 × 10- 06) were associated with serum FT3 level. Three SNPs (rs4850041 in LOC105373394-LINC01249: p = 3.55 × 10- 06, rs6867291 in LINC02208: p = 2.40 × 10- 06 and rs79508321 in WWOX: p = 3.35 × 10- 06) were related to circulating T3 level. Rs12474167 (LOC105373394-LINC01249, p = 1.65 × 10- 06) and rs1864553 (IWS1, p = 2.00 × 10- 06) were associated with circulating T4 concentration. The association with TGA concentration was for rs17163542 in DISP1 (p = 3.46 × 10- 06) and rs12601151 in NOG-C17orf67 (p = 2.72 × 10- 07). Two genome-level significant SNPs (rs2114707 in LINC01314, p = 1.69 × 10- 06 and rs12601151, p = 1.41 × 10- 07) associated with serum TMA concentration were identified. Moreover, rs6083269 (CST1-CST2, p = 3.36 × 10- 06) was a significant locus for circulating TSH level. In replication, rs12601151 in NOG-C17orf67 was still associated with serum TGA level (p = 0.012). Conclusions: The GWAS reported 11 new suggestively significant loci associated with circulating thyroid-related hormones levels among the Chinese Han population. These findings represented suggestively biological candidates for circulating thyroid-related hormones levels and provided new insights into the mechanisms of regulating serum TGA concentration.

Organic Acid Extraction of Fluoride from Antarctic Krill Euphausia superba

( Cheng Liang Xie ),( Han Soo Kim ),( Kil Bo Shim ),( Yeon Kye Kim ),( Na Yeong Yoon ),( Poong Ho Kim ),( Ho Dong Yoon ) 한국수산과학회(구 한국수산학회) 2012 Fisheries and Aquatic Sciences Vol.15 No.3

The amount of fluoride removed from Antarctic krill via organic acid extraction depends on the extraction time, concentration, extraction volume, and the nature of the acid itself. The fluoride content in Euphausia superba was determined by measuring the concentration of fluoride ion in acidic extracts using an ion-selective electrode. The best results were obtained by adding 50mL of 0.01 M citric acid to 0.3-0.5 g of knIt and extracting for 5 mm. Under these conditions, recoveries of fluoride from frozen whole bill and krill meat were 95.6-99, 5% and 97.5-101.3%, respectively. The dry basis fluoride contents of krill by-product, krill meat and the boiled knill were 705, 298, and 575ppm, respectively. These levels were significantly reduced by citric acid extraction.