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Liang-Liang Chen,Ge-Xin Gao,Fei-Xia Shen,Xiong Chen,Xiao-Hua Gong,Wen-Jun Wu 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.9
As the most common type of endocrine malignancy, papillary thyroid cancer (PTC) accounts for 85-90% of all thyroid cancers. In this study, we presented the hypothesis that SDC4 gene silencing could effectively attenuate epithelial mesenchymal transition (EMT), and promote cell apoptosis via the Wnt/β-catenin signaling pathway in human PTC cells. Bioinformatics methods were employed to screen the determined differential expression levels of SDC4 in PTC and adjacent normal samples. PTC tissues and adjacent normal tissues were prepared and their respective levels of SDC4 protein positive expression, in addition to the mRNA and protein levels of SDC4, Wnt/β-catenin signaling pathway, EMT and apoptosis related genes were all detected accordingly. Flow cytometry was applied in order to detect cell cycle entry and apoptosis. Finally, analyses of PTC migration and invasion abilities were assessed by using a Transwell assay and scratch test. In PTC tissues, activated Wnt/β-catenin signaling pathway, increased EMT and repressed cell apoptosis were determined. Moreover, the PTC K1 and TPC-1 cell lines exhibiting the highest SDC4 expression were selected for further experiments. In vitro experiments revealed that SDC4 gene silencing could suppress cell migration, invasion and EMT, while acting to promote the apoptosis of PTC cells by inhibiting the activation of the Wnt/β-catenin signaling pathway. Besides, si-β-catenin was observed to inhibit the promotion of PTC cell migration and invasion caused by SDC4 overexpression. Our study revealed that SDC4 gene silencing represses EMT, and enhances cell apoptosis by suppressing the activation of the Wnt/β-catenin signaling pathway in human PTC.
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Preparation of Ultrasensitive Humidity-Sensing Films by Aerosol Deposition
Liang, Jun-Ge,Wang, Cong,Yao, Zhao,Liu, Ming-Qing,Kim, Hong-Ki,Oh, Jong-Min,Kim, Nam-Young American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.1
<P>Aerosol deposition (AD) is a novel ceramic film preparation technique exhibiting the advantages of room-temperature operation and highly efficient film growth. Despite these advantages, AD has not been used for preparing humidity-sensing films. Herein, room-temperature AD was utilized to deposit BaTiO<SUB>3</SUB> films on a glass substrate with a Pt interdigital capacitor, and their humidity-sensing performances were evaluated in detail, with further optimization performed by postannealing at temperatures of 100, 200, ..., 600 °C. Sensor responses (i.e., capacitance variations) were measured in a humidity chamber for relative humidities (RHs) of 20-90%, with the best sensitivity (461.02) and a balanced performance at both low and high RHs observed for the chip annealed at 500 °C. In addition, its response and recovery were extremely fast, respectively, at 3 and 6 s and it kept a stable recording with the maximum error rate of 0.1% over a 120 h aging test. Compared with other BaTiO<SUB>3</SUB>-based humidity sensors, the above chip required less thermal energy for its preparation but featured a more than 2-fold higher sensitivity and a superior detection balance at RHs of 20-90%. Cross-sectional transmission electron microscopy imaging revealed that the prepared film featured a transitional variable-density structure, with moisture absorption and desorption being promoted by a specific capillary structure. Finally, a bilayer physical model was developed to explain the mechanism of enhanced humidity sensitivity by the prepared BaTiO<SUB>3</SUB> film.</P> [FIG OMISSION]</BR>
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Thickness effects of aerosol deposited hygroscopic films on ultra-sensitive humidity sensors
Liang, Jun-Ge,Kim, Eun-Seong,Wang, Cong,Cho, Myung-Yeon,Oh, Jong-Min,Kim, Nam-Young Elsevier 2018 Sensors and actuators. B Chemical Vol.265 No.-
<P><B>Abstract</B></P> <P>Hygroscopic film that has a super mesoporous structure exhibits high sensitivity and fast response/recovery in humidity sensing applications. Aerosol deposition (AD) via a shock-loading-solidification preparation mechanism, which is an unexplored potential hygroscopic film preparation technique, can produce a porous microstructure, and the AD hammering effect creates various interior and surface microstructures in different-thickness films. The objective of our study was to verify the feasibility of using AD as a humidity-sensing film preparation technique, and to investigate the thickness effects of the film on its microstructure and hygroscopic properties. Hygroscopic films with thicknesses ranging from 0.1–10.0 μm were aerosol deposited with BaTiO<SUB>3</SUB> powders before undergoing a 400 °C thermal treatment. The resulting surface morphology exhibited roughness increases for thicknesses from 0.1–6.0 μm, and a decrease at 10.0 μm. The cross-sectional structure was characterized by a transitional-density grain distribution in which higher density small-sized grains could be seen in the bottom layer. Films with thicknesses of 1.5 μm and 3.0 μm achieved excellent sensitivities of 178.6 ± 7.3 pF/%RH and 299.9 ± 5.4 pF/%RH, respectively. Further, the response and recovery time for the 1.5 μm film were both less than 5 s. The current study determined the feasibility of AD-based humidity-sensing film preparation and provided a reference for optimal thickness control. The surface hydrophilicity, pore volume, and open-pore ratio were analyzed as critical factors of the thickness related humidity sensing effects, and physical modeling indicated that AD-prepared films exhibited an expanded humidity detection range, enhanced water vapor adsorption and desorption, and improved sensitivity to humidity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Aerosol deposition can be used to prepare ultra-sensitive ceramic humidity sensors. </LI> <LI> Film-based sensors were prepared using a shock-loading solidification mechanism of aerosol deposition. </LI> <LI> Their sensitivity depends on the hydrophilicity, pore volume, and open-pore ratio. </LI> <LI> The thickness of the film regularly affects the humidity-sensing properties. </LI> </UL> </P>
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Protection Relay and Fault Information Analyzing System for Smart Grid
Ge, Liang,Wang, Liding,Yang, Changfu,Zhou, Shuxiong 대한전기학회 2013 The Journal of International Council on Electrical Vol.3 No.2
For smart grid, the fault information from protection relays and fault recorders is useful for dispatchers to identify the grid fault and resume the power supply fleetly when a fault occurs. Traditionally, EMS/SCADA doesn't have the ability to collect the fault information. An application of protection relay and fault information analyzing system is proposed. In order to implement smarter analyzing functions, three approaches are adopted. First, the system unifies IEC61850 and IEC103 for modeling. Second, an analyzing method of relay characteristic is proposed to evaluate the action behavior of protection relays. Finally, the paper presents an approach to replay the actual action sequence with relay's logic diagram. The system has been carried out and the paper concludes that the system is effective and useful for dispatchers and relay operators in smart grid.
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한국과 중국 대학생의 전통혼례복에 대한 가치 인식과 선호도가 양국의 수입 패션제품 구매의도에 미치는 영향
갈량(Ge Liang),황춘섭(Hwang Choon-Sup) 한복문화학회 2015 韓服文化 Vol.18 No.3
The purpose of this study was to analyze Korean and Chinese college students’ value perception and preference for their traditional wedding costumes, and also to analyze the influence of their value perception and preference on purchase intention for imported fashion products. The information regarding the relationship between the value perception of traditional wedding costumes and the purchase intention for imported fashion products would provide some basic data required to establish differentiated marketing strategy in the trading between both countries. The study was implemented through a descriptive survey method using a questionnaire. The sample of the study consisted of 200 Korean and 200 Chinese college students who are currently enrolled in a university located in Seoul. The data were analyzed by factor analysis and Cronbach’s α coefficients, ANOVA, and regression analysis. The results revealed that there were no significant differences with respect to Korea and China in college students’ value perception of their traditional wedding costumes. College students from both countries valued their traditional wedding costumes considerably high. While respondents from both countries expressed high preference for traditional wedding costumes in the aspect of design, differences were found between Korea and China in the preference level for traditional wedding costumes in the aspect of their symbolic value. Chinese students displayed a higher preference level for the symbolic value of traditional wedding costumes than Korean students. The results also revealed that students’ value perception and preference for their traditional wedding costumes have influence on purchase intention for imported fashion products in both countries. Students with a higher level of value perception and preference for traditional wedding costumes expressed a lower purchase intention for imported fashion products. This fact would be meaningful in understanding the global consumption culture acceptance level of the two countries.
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Chen, Liang-Liang,Gao, Ge-Xin,Shen, Fei-Xia,Chen, Xiong,Gong, Xiao-Hua,Wu, Wen-Jun Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.9
As the most common type of endocrine malignancy, papillary thyroid cancer (PTC) accounts for 85-90% of all thyroid cancers. In this study, we presented the hypothesis that SDC4 gene silencing could effectively attenuate epithelial mesenchymal transition (EMT), and promote cell apoptosis via the $Wnt/{\beta}-catenin$ signaling pathway in human PTC cells. Bioinformatics methods were employed to screen the determined differential expression levels of SDC4 in PTC and adjacent normal samples. PTC tissues and adjacent normal tissues were prepared and their respective levels of SDC4 protein positive expression, in addition to the mRNA and protein levels of SDC4, $Wnt/{\beta}-catenin$ signaling pathway, EMT and apoptosis related genes were all detected accordingly. Flow cytometry was applied in order to detect cell cycle entry and apoptosis. Finally, analyses of PTC migration and invasion abilities were assessed by using a Transwell assay and scratch test. In PTC tissues, activated $Wnt/{\beta}-catenin$ signaling pathway, increased EMT and repressed cell apoptosis were determined. Moreover, the PTC K1 and TPC-1 cell lines exhibiting the highest SDC4 expression were selected for further experiments. In vitro experiments revealed that SDC4 gene silencing could suppress cell migration, invasion and EMT, while acting to promote the apoptosis of PTC cells by inhibiting the activation of the $Wnt/{\beta}-catenin$ signaling pathway. Besides, $si-{\beta}-catenin$ was observed to inhibit the promotion of PTC cell migration and invasion caused by SDC4 overexpression. Our study revealed that SDC4 gene silencing represses EMT, and enhances cell apoptosis by suppressing the activation of the $Wnt/{\beta}-catenin$ signaling pathway in human PTC.
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Ying-Hua Liang,Ping-Zhan Si,Ting-Ting Qi,Xin-You Wang,Fei-Yang Wang,Qiong Wu,Hong-Liang Ge,Jihoon Park,Chul-Jin Choi 한국자기학회 2024 Journal of Magnetics Vol.29 No.1
Both Mn₄C (=Mn₃MnC) and Mn₃GaC have been studied previously. However, the reports on Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1) with intermediate compositions are very rare. In this work, the structure and magnetic properties of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C prepared by using solid state reaction were studied systematically. High purity anti-perovskitetype Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C were obtained in the composition range of 0 ≤ x ≤ 0.5, above which Mn₂₃C₆ precipitates and the fraction of Mn₂₃C₆ in the samples increases with increasing x. The structural stability, lattice parameters, and room temperature saturation magnetization of ferromagnetic Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1) decreases with increasing x. The Curie temperature of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1) increases with increasing x. Most Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C with varied x exhibit near-zero coercivity and zero remanent magnetization. This work indicates that the temperature coefficient of magnetization of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C may be tuned by tuning the fraction of the Ga atoms.
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Effect of post weld treatment on cracking behaviors of beam-column connections in steel bridge piers
Liang-Jiu Jia,Hanbin Ge,Toshimitsu Suzuki 국제구조공학회 2014 Steel and Composite Structures, An International J Vol.17 No.5
A great number of moment-resisting steel structures collapsed due to ductile crack initiation at welded beam-column connections, followed by explosive brittle fracture in the Kobe (Hyogoken-Nanbu) earthquake in 1995. A series of experimental and numerical studies on cracking behaviors of beam-column connections in steel bridge piers were carried out by the authors’ team. This paper aims to study the effect of post weld treatment on cracking behaviors of the connections during a strong earthquake event. Experiments of three specimens with different weld finishes, i.e., as-welded, R-finish, and burr grinding, were conducted. The experimental results indicate that the instants of ductile crack initiation are greatly delayed for the specimens with R-finish and burr grinding finishes compared with the as-welded one. The strain concentration effect in the connection is also greatly reduced in the specimens with post weld treatment compared with the as-welded one, which was also verified in the tests.
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Ge Qi,Xiao Rui,Yuan Yi,He Suqun,Chen Liang,Ma Shangshang,Taha Rehab Hosny,Yao Qin,Chen Keping 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4
The silkworm, Bombyx mori, is an important model economic insect. Cross breeding is an important way to select excellent silkworm varieties, and different combinations among the same parents often show different genetic characters. To explore the regulatory mechanism of silk gland differences in reciprocal cross silkworm, the 3rd day of the 5th instar of the orthogonal silkworm (306♀ × NB♂, 798♀ × NB♂) and the reverse cross silkworm (NB♀ × 306♂, NB♀ × 798♂) silk glands were used as experimental materials, then using Label-free-based proteomics and RNA-Seq-based transcriptomics for analysis. Besides, the qRT-PCR was used to validate the expression of differentially expressed genes. The results showed that in NB♀ × 306♂ VS 306♀ × NB♂ group, 280 differentially expressed genes and 238 differential proteins were up-regulated while 40 differentially expressed genes and 149 differential proteins were down-regulated; in NB♀ × 798♂ VS 798♀ × NB♂, 82 differentially expressed genes and 325 differential proteins were up-regulated while 113 differentially expressed genes and 258 differential proteins were down-regulated. Further analysis indicated that the up-regulation of mitochondrial aldehyde dehydrogenase gene and cytochrome P450 gene, which are related to mitochondria, might be regulated by maternal inheritance. The GO and KEGG enrichment initially showed that the differential genes and proteins are mainly involved in processes such as protease metabolism, ATP hydrolysis, lipid transport, insect hormone synthesis, and TCA cycle. The down-regulated expression of ecdysone oxidase and Serpin-32 in the reverse cross group will reduce the energy utilization in the process of silk gland synthesis, increase the hydrolysis of serine, affect the synthesis and transport of silk protein, and cause the difference between reciprocal crosses.
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