http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Proteomic Analysis of the Aging-related Proteins in Human Normal Colon Epithelial Tissue
Li, Ming,Xiao, Zhi-Qiang,Chen, Zhu-Chu,Li, Jian-Ling,Li, Cui,Zhang, Peng-Fei,Li, Mao-Yu Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.1
In order to screen the aging related proteins in human normal colon epithelia, the comparative proteomics analysis was applied to get the two-dimensional electrophoresis (2-DE) profiles with high resolution and reproducibility from normal colon epithelial tissues of young and aged people. Differential proteins between the colon epithelia of two age groups were found with PDQuest software. The thirty five differential protein-spots were identified by peptide mass fingerprint (PMF) based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and database searching. Among them there are sixteen proteins which are significantly up-regulated in the colonic mucosal epithelia of young people group, which include ATP synthase beta chain, electron transfer flavoprotein alpha-subunit, catalase, glutathione peroxidase 1, annexin A2 and heat shock cognate 71 kDa protein, etc.; There are nineteen proteins which are significantly up-regulated in the colonic mucosal epithelia of aged people group, which include far upstream element-binding protein 1, nucleoside diphosphate kinase B, protein disulfide-isomerase precursor and VDAC-2, etc.. The identified differential proteins appear to be involved in metabolism, energy generation, chaperone, antioxidation, signal transduction, protein folding and apoptosis. The data will help to understand the molecular mechanisms of human colon epithelial aging.
Shi, Qing-Qiang,Zuo, Guo-Wei,Feng, Zi-Qiang,Zhao, Lv-Cui,Luo, Lian,You, Zhi-Mei,Li, Dang-Yang,Xia, Jing,Li, Jing,Chen, Di-Long Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18
Purpose: To investigate the effect of deacetylase inhibitory trichostatin A (TSA) on anti HepG2 liver carcinoma cells and explore the underlying mechanisms. Materials and Methods: HepG2 cells exposed to different concentrations of TSA for 24, 48, or 72h were examined for cell growth inhibition using CCK8, changes in cell cycle distribution with flow cytometry, cell apoptosis with annexin V-FTIC/PI double staining, and cell morphology changes under an inverted microscope. Expression of ${\beta}$-catenin, HDAC1, HDAC3, H3K9, CyclinD1 and Bax proteins was tested by Western blotting. Gene expression for ${\beta}$-catenin, HDAC1and HDAC3 was tested by q-PCR. ${\beta}$-catenin and H3K9 proteins were also tested by immunofluorescence. Activity of Renilla luciferase (pTCF/LEF-luc) was assessed using the Luciferase Reporter Assay system reagent. The activity of total HDACs was detected with a HDACs colorimetric kit. Results: Exposure to TSA caused significant dose-and time-dependent inhibition of HepG2 cell proliferation (p<0.05) and resulted in increased cell percentages in G0/G1 and G2/M phases and decrease in the S phase. The apoptotic index in the control group was $6.22{\pm}0.25%$, which increased to $7.17{\pm}0.20%$ and $18.1{\pm}0.42%$ in the treatment group. Exposure to 250 and 500nmol/L TSA also caused cell morphology changes with numerous floating cells. Expression of ${\beta}$-catenin, H3K9and Bax proteins was significantly increased, expression levels of CyclinD1, HDAC1, HDAC3 were decreased. Expression of ${\beta}$-catenin at the genetic level was significantly increased, with no significant difference in HDAC1and HDAC3 genes. In the cytoplasm, expression of ${\beta}$-catenin fluorescence protein was not obvious changed and in the nucleus, small amounts of green fluorescence were observed. H3K9 fluorescence protein were increased. Expression levels of the transcription factor TCF werealso increased in HepG2 cells following induction by TSA, whikle the activity of total HDACs was decreased. Conclusions: TSA inhibits HDAC activity, promotes histone acetylation, and activates Wnt/${\beta}$-catenin signaling to inhibit proliferation of HepG2 cell, arrest cell cycling and induce apoptosis.
Correlations of Tumor-associated Macrophage Subtypes with Liver Metastases of Colorectal Cancer
Cui, Yun-Long,Li, Hui-Kai,Zhou, Hong-Yuan,Zhang, Ti,Li, Qiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Objective: This work aimed to investigate the correlations of tumor-associated macrophages (TAMs) and their subtypes M1 and M2 with liver metastasis of colorectal cancer, and provide useful references for seeking predictors of liver metastasis and studying mechanisms. Methods: 120 patients with colorectal cancer from 2000 to 2009 were divided into low, middle and high liver metastasis groups (group A, B and C, respectively). S-P immunohistochemical staining and microscopic observation were conducted to compare expression in CD68-positive cells (TAMs), CD80-positive cells (M1) and CD163-positive cells (M2) in three groups. Correlations of TAMs, M1, M2, and M2/M1 ratio with clinical and pathological parameters were analyzed. Results: With increase of liver metastatic ability, the number of TAMs decreased gradually, with no significant difference between any two of the three groups (P > 0.05), while the numbers of M1 and M2 were significantly decreased and increased, respectively, with significant difference between any two of three groups (P < 0.05 or P < 0.01). In addition, the M2/M1 ratio increased with increase of liver metastatic ability (P < 0.01). There was no statistical significance of correlation of TAMs with each clinical and pathological parameter. M1 was negatively related with lymphatic metastasis and liver metastatic ability. M2 was positively correlated with preoperative CEA level, lymphatic metastasis, tumor differentiation degree and liver metastatic ability. The same was the case for the M2/M1 ratio. Conclusions: Effects of TAMs on liver metastasis of colorectal cancer do not depend on the total number of TAMs, but on the number and proportion of functional subtypes M1 and M2. M2 number and M2/M1 ratio are more accurate predictors for liver metastasis of colorectal cancer.
Cui-Ping Li,Jia-Qiang Wang,Jun Lin,Yan Shi,Zhi-Feng Fu 한국고분자학회 2011 Macromolecular Research Vol.19 No.8
The free radical polymerization of styrene was carried out in the presence of a new found chain transfer monomer, p-vinyl benzene sulfonyl chloride (VBSC), which possesses both a chain transfer group and a polymerizable double bond. Branched polystyrene was formed during the polymerization, as indicated by multi-peaks gel permeation chromatography (GPC) curves of the products, the increase in the number-average molecular weight and molecular weight distribution along with monomer conversions. The structure of the obtained polystyrene was analyzed by 1H nuclear magnetic resonance (NMR) spectroscopy. The results showed that with increasing VBSC in the feed, the degree of branching and VBSC unit in the copolymer increased and a shortest polystyrene arm arose from the highest VBSC content in the feed, suggesting that the composition and structure of the branched polystyrenes could be tuned by the amount of VBSC in the feed. By tracing the structure change in the copolymer at various stages of polymerization, the main polymerization process can be regarded as the copolymerization of VBSC with styrene first and then chain transfer to polymeric radical to form branched polystyrene. This strategy is facile and less expensive than the other method.
Regeneration of commercial SCR catalyst deactivated by arsenic poisoning in coal-fired power plants
Qiang Lu,Zulfiqar Ali,Hao Tang,Tahir Iqbal,Zulqarnain Arain,Min-shu Cui,Ding-jia Liu,Wen-yan Li,Yong-ping Yang 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.3
Arsenic species, which are inevitable components in flue gas from the coal combustion process, will result in severe deactivation of selective catalytic reduction (SCR) catalysts. In this paper, a novel method is proposed to regenerate the arsenic-poisoned commercial V2O5-MoO3/TiO2 catalyst collected from coal-fired power plants, including ammonia washing, H2 reduction, and air calcination. Activity tests indicated that the proposed method could recover the catalyst activity more than 96% of the fresh catalyst. Furthermore, detailed characterizations results indicated that this regeneration method could not only effectively remove the arsenic species, but also recover the active constituents of the catalysts to a considerable level. The proposed method offers a feasible strategy for the regeneration of poisoned commercial SCR catalysts and can effectively reduce the total denitrification cost for coal-fired power plants.
Qiang Cui,Xibo Wang,Guorong Wang,Rui Li,Xiaodan Wang,Shuang Chen,Jingnan Liu,Lianzhou Jiang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.5
This paper studied the influences of diverse ultrasonic power treatments on the physico-chemical properties of soy–whey mixed protein induced by microbial transglutaminase. Two groups of 15% (m/v) of protein solution-sole protein (as control group) and mixed protein were prepared and processed under different ultrasonic powers for 30 min. After ultrasonic power treatments, gel properties were significantly increased: under 300 W, the gel hardness of mixed protein reached a maximum of 998.9 g, with its water binding capacity scoring a maximum of 87%. According to the analysis of fluorescence emission spectrum, the fluorescence intensity and maximum absorption peak had changed, for different ultrasonic power treatments had exposed more groups. The Fourier Transform Infrared Spectroscopy also suggested that ultrasonic power treatments could change the secondary structure of gel samples. The scanning electron microscope demonstrated that the network structure of mixed protein gel displayed more regular and uniform after ultrasonic treatments.
Cui-Ping Li,Jia-Qiang Wang,Yan Shi,Zan Liu,Jun Lin,Zhi-Feng Fu 한국고분자학회 2012 Macromolecular Research Vol.20 No.8
Branched polystyrene was first obtained via a reversible addition-fragmentation chain transfer (RAFT)process in the presence of chain transfer monomer p-vinyl benzene sulfonyl chloride (VBSC) in benzene at 60 oC with 2-(ethoxycarbonyl)prop-2-yl dithiobenzoate as the RAFT agent and 2,2-azobisisobutyronitrile as an initiator. During the RAFT polymerization, VBSC played the role of branching agent. It could not only copolymerized but also acted as a chain transfer agent due to the polymerizable vinyl group and sulfonyl chloride chain transfer group in the VBSC. Gel permeation chromatography (GPC) traces demonstrated that the number-average molecular weights and molecular weight distributions increased along with monomer conversion. Compared with the RAFT process without VBSC, the resulting polymers had broad molecular weight distributions and the sulfonyl functionality of the resultant polymer at the branching point, indicating the formation of branched polystyrene. The structure of the obtained polystyrene was further analyzed by proton nuclear magnetic resonance (1H NMR) spectroscopy. The findings indicated that the branched polystyrene was mainly formed via the RAFT copolymerization of VBSC and styrene firstly to form polystyrene bearing pendant sulfonyl chloride group, and then the pendant sulfonyl chloride group acted as the chain transfer agent to generate the branched structure. In addition, the degree of branching and VBSC unit in copolymer increased along with the VBSC in the feed, implying that composition and structure of the branched polystyrenes could be tuned by the amount of VBSC in the feed.