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        The Production and Cytological Analysis of Brassica napus -B Genome Chromosome Monosomic Addition Lines and Their Hybrids

        Li Maoteng,Xiang Jun,Liu Jianmin,Yu Longjiang,Li Dianrong 한국유전학회 2008 Genes & Genomics Vol.30 No.2

        The Brassica napus-B genome monosomic addition lines (MALs) (AACC + B’, 2n = 39) were developed from self-pollination of pentaploid hybrids (AABCC) that were derived from hybridization between hexaploid hybrids (AABBCC) and B. napus (AACC). The alien chromosomes of the B genome in MALs were identified by the GISH technique, by observation of the meiotic behavior of pollen mother cells (PMCs), and by B-genome-specific molecular marker analysis. Studies of the meiotic behavior of B. napus-B genome chromosome MALs at diakinesis revealed that the majority of the chromosome configuration was 19²+1¹, which indicated that the alien B genome chromosome remained univalent in most cases. The laggard-free PMCs also appeared at a lower ratio, which indicated that the B genome chromosome could be transmitted into gametes. The chromosome configurations of 20² and 19²+2¹ that appeared in double MALs (AACC+ 2 chromosomes of the B genome) indicated different homoeology between different B genome chromosomes. The paired B genome bivalent in double MALs can be normally segregated at anaphase in most cases. PMCs with multivalents were observed in all the double MAL combinations, which indicated homology of the B genome chromosomes with the A or C genome chromosomes. The Brassica napus-B genome monosomic addition lines (MALs) (AACC + B’, 2n = 39) were developed from self-pollination of pentaploid hybrids (AABCC) that were derived from hybridization between hexaploid hybrids (AABBCC) and B. napus (AACC). The alien chromosomes of the B genome in MALs were identified by the GISH technique, by observation of the meiotic behavior of pollen mother cells (PMCs), and by B-genome-specific molecular marker analysis. Studies of the meiotic behavior of B. napus-B genome chromosome MALs at diakinesis revealed that the majority of the chromosome configuration was 19²+1¹, which indicated that the alien B genome chromosome remained univalent in most cases. The laggard-free PMCs also appeared at a lower ratio, which indicated that the B genome chromosome could be transmitted into gametes. The chromosome configurations of 20² and 19²+2¹ that appeared in double MALs (AACC+ 2 chromosomes of the B genome) indicated different homoeology between different B genome chromosomes. The paired B genome bivalent in double MALs can be normally segregated at anaphase in most cases. PMCs with multivalents were observed in all the double MAL combinations, which indicated homology of the B genome chromosomes with the A or C genome chromosomes.

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        A tubular linear motor structure suitable for large thrust

        Wenbin Yu,Guolai Yang,Zixuan Li,Dianrong Wang,Xiuye Wang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.11

        In response to the problem that the motor shaft of conventional tubular linear motor is prone to bending, this paper proposes a new tubular linear motor structure for large thrust demand. Bending of the motor shaft can lead to collisions between structures and affect motor performance, and traditional air-gap structures cannot effectively handle this problem. The stator of the new motor is divided into an inner and outer cylinder structure. The armature windings are arranged between the inner and outer cylinders. The permanent magnets are arranged on the motor shaft. After analysis, the structural proposed in this paper can well stop the bending of the motor shaft. It also effectively improves the problem of large fluctuations in the thrust of linear motors. Finally, it is concluded that the peak motor thrust is maximized when the material with low permeability is chosen for the inner cylinder.

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