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RISS 인기검색어
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- 저자 : Leping Dang (검색결과 2 건)
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Ionic Liquids as Selectors for Controlling the Crystallization Nucleation of Hen Egg White Lysozyme
Zhanzhong Wang,Qian Wang,Leping Dang 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.5
Ionic liquids (ILs) have biomaterial applications and are used for protein crystallization. The effect of two imidazolium-based ILs, 1-butyl-3-methylimidazolium chloride ([C4mim]Cl) and 1,3-dimethylimidazolium iodide ([dmim]I),on the nucleation kinetics of lysozyme was investigated by determining the nucleation induction time, and nucleation parameters were evaluated. The values of interfacial tension calculated for solutions with added 30 g/L ILs [C4mim]Cl and [dmim]I, and without added ILs were 99.03, 109.7, and 107.3 mJ/m2, respectively. Compared with solutions without IL addition, the critical free energy change, size, and molecular number of critical nuclei decreased and the nucleation rate increased after the addition of [C4mim]Cl. In contrast, the critical free energy change, size, and molecular number of critical nuclei increased and the nucleation rate decreased after the addition of [dmim]I. These new findings provide insights into controlling lysozyme crystallization separation, and present ILs as potentially useful additives for controlling the crystallization of macromolecules.
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Nucleation behavior of glutathione polymorphs in water
Hongyuan Wei,Zhi Chen,Leping Dang,Shuai Li 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.10
Nucleation behavior of glutathione (GSH) polymorphs in water was investigated by experimental method combined with classical nucleation theory. The solubility of α and β forms GSH in water at different temperatures,and the nucleation induction period at various supersaturations and temperatures were determined experimentally. The results show that, in a certain range of supersaturation, the nucleation of β form predominates at relatively higher temperature,while α form will be obtained at lower temperature. The nucleation kinetics parameters of α and β form were then calculated. To understand the crucial role of temperature on crystal forms, “hypothetic” nucleation parameters of β form at 283.15 K were deduced based on extrapolation method. The results show that the interfacial tension, critical free energy, critical nucleus radius and nucleus number of α form are smaller than that of β form in the same condition at 283.15 K, which implies that α form nucleates easier than β form at low temperature. This work may be useful for the control and optimization of GSH crystallization process in industry.
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