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GLI1 Transcription Factor Affects Tumor Aggressiveness in Patients With Papillary Thyroid Cancers
Lee, Jandee,Jeong, Seonhyang,Lee, Cho Rok,Ku, Cheol Ryong,Kang, Sang-Wook,Jeong, Jong Ju,Nam, Kee-Hyun,Shin, Dong Yeob,Chung, Woong Youn,Lee, Eun Jig,Jo, Young Suk Williams & Wilkins Co 2015 Medicine Vol.94 No.25
<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>A significant proportion of patients with papillary thyroid cancer (PTC) present with extrathyroidal extension (ETE) and lymph node metastasis (LNM). However, the molecular mechanism of tumor invasiveness in PTC remains to be elucidated.</P><P>The aim of this study is to understand the role of Hedgehog (Hh) signaling in tumor aggressiveness in patients with PTC.</P><P>Subjects were patients who underwent thyroidectomy from 2012 to 2013 in a single institution. Frozen or paraffin-embedded tumor tissues with contralateral-matched normal thyroid tissues were collected. Hh signaling activity was analyzed by quantitative RT-PCR (qRT-PCR) and immunohistochemical (IHC) staining. Datasets from Gene Expression Omnibus (GEO) (National Center for Biotechnology Information) were subjected to Gene Set Enrichment Analysis (GSEA). BRAFT1799A and telomerase reverse transcriptase promoter mutation C228T were analyzed by direct sequencing.</P><P>Among 137 patients with PTC, glioma-associated oncogene homolog 1 (<I>GLI1</I>) group III (patients in whom the ratio of <I>GLI1</I> messenger ribonucleic acid (mRNA) level in tumor tissue to <I>GLI1</I> mRNA level in matched normal tissue was in the upper third of the subject population) had elevated risk for ETE (odds ratio [OR] 4.381, 95% confidence interval [CI] 1.414–13.569, <I>P</I> = 0.01) and LNM (OR 5.627, 95% CI 1.674–18.913, <I>P</I> = 0.005). Glioma-associated oncogene homolog 2 (<I>GLI2</I>) group III also had elevated risk for ETE (OR 4.152, 95% CI 1.292–13.342, <I>P</I> = 0.017) and LNM (OR 3.924, 95% CI 1.097–14.042, <I>P</I> = 0.036). GSEA suggested that higher <I>GLI1</I> expression is associated with expression of the <I>KEGG</I> gene set related to axon guidance (<I>P</I> = 0.031, false discovery rate < 0.05), as verified by qRT-PCR and IHC staining in our subjects.</P><P><I>GLI1</I> and <I>GLI2</I> expressions were clearly related to aggressive clinicopathological features and aberrant activation of GLI1 involved in the axon guidance pathway. These results may contribute to development of new prognostic markers, as well as novel therapeutic targets.</P></▼2>
Association Between Obesity and BRAFV600E Mutation Status in Patients with Papillary Thyroid Cancer
Lee, Jandee,Lee, Cho Rok,Ku, Cheol Ryong,Kang, Sang-Wook,Jeong, Jong Ju,Shin, Dong Yeob,Nam, Kee-Hyun,Jung, Sang Geun,Lee, Eun Jig,Chung, Woong Youn,Jo, Young Suk Springer - Society of Surgical Oncology 2015 Annals of Surgical Oncology Vol.22 No.suppl3
Sang Kun Park,Jin Hee Lim,Dong Woo Lee,Hae Ryong Cho,Hye Kyung Rhee,Mi Seon Kim,Hyang Young Joung,Seong Yeol Choi 한국육종학회 2007 한국육종학회지 Vol.39 No.4
A new gerbera variety ‘Claret’ was released by the National Horticultural Research Institute (NHRI) in 2006. A cross was made between ‘Rako’ with pink and semi-double and ‘Sessile’ with orange and single in 2000. After investigation of the characteristics for 6 years (from 2000 to 2006), it was selected specially for the use of cut-flower. ‘Claret’ is vigorous cultivar with purple and semi-double. It produces a large yield with continuous supply of flowers and has adequate peduncle height and brilliant color. We believe that ‘Claret’ has a great potential for expanding cultivated area of domestic mini-gerbera cultivar and increasing farmers-income. A new gerbera variety ‘Claret’ was released by the National Horticultural Research Institute (NHRI) in 2006. A cross was made between ‘Rako’ with pink and semi-double and ‘Sessile’ with orange and single in 2000. After investigation of the characteristics for 6 years (from 2000 to 2006), it was selected specially for the use of cut-flower. ‘Claret’ is vigorous cultivar with purple and semi-double. It produces a large yield with continuous supply of flowers and has adequate peduncle height and brilliant color. We believe that ‘Claret’ has a great potential for expanding cultivated area of domestic mini-gerbera cultivar and increasing farmers-income.
Lee, Suk-Ha,Agashe, Mandar V.,Suh, Seung-Woo,Yoon, Yong-Cheol,Song, Sang-Heon,Yang, Jae-Hyuk,Lee, Hanna,Song, Hae-Ryong Lippincott Williams & Wilkins 2012 Spine Vol.37 No.13
STUDY DESIGN.: A retrospective observational study. OBJECTIVE.: The objective of this study was to analyze the cases of paravertebral ossification in vitamin D–resistant rickets (VDRR) and elucidate its incidence and clinical significance, and report specific genetic mutations uniquely associated with this phenomenon. SUMMARY OF BACKGROUND DATA.: Paravertebral ligament ossification has been described in VDRR in a few cases previously. However, supraspinous and interspinous ligament ossification has so far not been described to occur in association with VDRR. We reviewed 6 cases of paravertebral ligament ossification with the added feature of supraspinous and interspinous ligament calcification. METHODS.: Forty-four patients with clinical, radiological, and biochemical diagnoses of VDRR, who were positive for mutations in the PHEX gene, were screened for the presence of paravertebral ossification with plain radiographs and computed tomography/magnetic resonance imaging if necessary. This was correlated with the severity of disease, and we looked for specific genetic mutations. RESULTS.: Six patients had paravertebral ligament ossification, which included supraspinous and interspinous ligament ossification. These patients had a more severe systemic disease, with all patients requiring at least 1 surgery for deformity correction of the limbs, with a total of 10 surgical procedures performed in these 6 patients. c.1601C>T, c.1699C>T, c.1363G>T, and c.466_467insAC were the genetic mutations associated with these cases. CONCLUSION.: The spinal changes with paravertebral ligament ossification in VDRR were described in this study. Four different gene mutations related to the PHEX gene were detected in them. The presence of these changes was suggestive of a more severe systemic disease.
Lee, Kyunghee,Kim, Hyunsoo,Kim, Jin-Man,Kim, Jae-Ryong,Kim, Keuk-Jun,Kim, Yong-Jin,Park, Se-Il,Jeong, Jae-Ho,Moon, Young-mi,Lim, Hyun-Sook,Bae, Dong-Won,Kwon, Joseph,Ko, Chang-Yong,Kim, Han-Sung,Shin, Blackwell Publishing Ltd 2011 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.15 No.10
<P>Systemic transplantation of adipose-derived stem cells (ASCs) is emerging as a novel therapeutic option for functional recovery of diverse damaged tissues. This study investigated the effects of systemic transplantation of human ASCs (hASCs) on bone repair. We found that hASCs secrete various bone cell-activating factors, including hepatocyte growth factor and extracellular matrix proteins. Systemic transplantation of hASCs into ovariectomized mice induced an increased number of both osteoblasts and osteoclasts in bone tissue and thereby prevented bone loss. We also observed that conditioned medium from hASCs is capable of stimulating proliferation and differentiation of osteoblasts <I>via</I> Smad/extracellular signal-regulated kinase (ERK)/JNK (c-jun NH<SUB>2</SUB>-terminal kinase) activation as well as survival and differentiation of osteoclasts <I>via</I> ERK/JNK/p38 activation <I>in vitro</I>. Overall, our findings suggest that paracrine factors secreted from hASCs improve bone repair and that hASCs can be a valuable tool for use in osteoporosis therapy.</P>