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Pia Marie Albano,Christianne Salvador,Jose Orosa, III,Sheryl Racelis,Modesty Leaño,Angelika Michel,John Donnie Ramos,Dana Holzinger,Michael Pawlita 대한병리학회 2019 Journal of Pathology and Translational Medicine Vol.53 No.5
Background: The low prevalence of human papillomavirus (HPV) DNA and mRNA in biopsy samples of Filipinos with head and neck squamous cell carcinoma (HNSCC) has been reported previously. Here, the HPV serologic profiles of HNSCC cases were analyzed and associated with lifestyle and sexual practices. Methods: Serum samples were collected between May 2012 and September 2013 from HNSCC patients (n = 22) in the northwest region of the Philippines, and age- and sex-matched clinically healthy controls. Antibodies to capsid and early oncoproteins of HPV16, 18, 31, 33, 45, 52, 58, 6, and 11 were analyzed using multiplex serology. Results: Most of the cases were males with tumors of the oral cavity or larynx. Two of the cases tested positive for at least one of the early oncoproteins (E6, E7, E1, and/or E2) of HPV16, and 11 did not display reactivity to any HPV early or late oncoproteins. Of the controls, four tested positive for at least one of the HPV16 early oncoproteins, and 10 were non-reactive to all HPV types. Titers to HPV16 E6 or E7 of the seropositive cases and controls were considerably lower than those typically observed in economically developed countries. Conclusions: The low HPV titers seen here are consistent with the results of molecular analyses for this population. Hence, the seropositivity of some of the HNSCC cases is likely an indication of prior exposure to the virus and not the presence of HPV-driven tumors.
재조합 플라스미드 pSC3 에 단리된 효모 유전자 hom3 의 염색체 통합에 관한 연구
이호주,이우영,최승일 한국유전학회 1990 Genes & Genomics Vol.12 No.1
Aspartokinase (EC 2.7.2.4) is encoded by the HOM3 gene, a putative clone of which was previously isolated on a recombinant plasmid pSC3 (15.5 kbp) by its hom3 complementing ability in the yeast (Choi and Lea, 1988). The complementing sequence was further localized to the 2.15 kbp BamHI-Bg1II fragment by subcloning it from the pSC3. A replicating vector YRp7 to yield the plasmid pSC35. In an attempt to yield the integrative plasmid pSC351, the EcoRI fragment containing ARS-TRP1 was removed from pSC35. Mitotically stable integrative transformants were stable integrative transformants were selected upon introducing the linearized pSC351 into the original yeast recipient strain and crossed with an appropriate yeast strain for tetrad analysis. As a result, the integrated pSC351 DNA was positioned to, the expected genetic distance of 3.9 cM from the HIS1 locus on the right arm of yeast chromosome V, indicating that the 2.15 kbp fragment in pSC35 contains the entire functional HOM3 gene of the yeast, Saccharomyces cerevisiae.
Lea, Ho Zoo,Lea, Woo Young 한국균학회 1987 韓國菌學會誌 Vol.15 No.2
The yeast gene THR4 encodes threonine synthase(THS) which catalyses the last step(O-phosphohomoserine to and from threonine) of threonine biosynthesis pathway. The THR4 gene is one of the most poorly understood genes in yeast amino acid biosynthesis. Assignment of THR4 to THS is by inference without enzyme assay. The regulation circuitry of THR4 activity has not been investigated by classical biochemical methods. Isolated THR4 gene will provide an effective and powerful system for the study of molecular genetic mechanism for regulation of the gene expression. Three recombinant plasmids(pYLI: 22.0 Kb, pYL2: 16.5 Kb, and pYL3: 15.5 Kb) have been cloned into. E. coli from yeast genomic library(Vector: YCp50, 8.0 Kb) through their complementing activity of THR4 mutation in a recipient yeast strain. Organization of the plasmids was characterized through delineation of restriction cleavage sites in the insert fragments.
Lea, Ho Zoo,Lea, Woo Young 한국유전학회 1986 Genes & Genomics Vol.8 No.4
Isolated yeast genes for amino acid biosynthesis provide ideal and powerful system for the investigation of such molecular genetic mechanisms as the control of gene expression, especially when the activity of gene products is not easily be monitored by classical biochemical approaches. THR4 yeast gene has been defined only through mendelian genetic analysis and assumed to encode for threonine synthase (THS), which should catalyse the last step of threonine biosynthesis pathway; interconversion of 0-phosphohomoserine and threonine. The enzyme THS has never been directly assayed or characterized. Several recombinant plasmids (pYL series) have been isolated from yeast genomic library (vector; 8.0kb YCp50) by complementation of thr4 mutation in a recipient yeast strain M21-59 (thr4 trp1 ura3). Digestion fragments by 29 restriction endoncleases were analyzed within the 7.5kb insert region of pYL3 (15.5kb), which appeared to contain the thr4 complementing DNA. Twenty-three recognition sites in the insert have been delineated for the following restriction enzymes: BamHI (1 site), ClaI (1), EcoRI (1), EcoRV (3), KpnI (1), NruI (1), NsiI (1), PstI (2), PvuII (2), SacI (2), SacII (1), SalI (2), XbaI (2) and XhoI (3).
Monolithic zirconia crowns: effect of thickness reduction on fatigue behavior and failure load
Lea Sophia Prott,Frank Akito Spitznagel,Estevam Augusto Bonfante,Meike Anne Malassa,Petra Christine Gierthmuehlen 대한치과보철학회 2021 The Journal of Advanced Prosthodontics Vol.13 No.5
PURPOSE. The objective of this study was to evaluate the effect of thickness reduction and fatigue on the failure load of monolithic zirconia crowns. MATERIALS AND METHODS. 140 CAD-CAM fabricated crowns (3Y-TZP, inCorisTZI, Dentsply-Sirona) with different ceramic thicknesses (2.0, 1.5, 1.0, 0.8, 0.5 mm, respectively, named G2, G1.5, G1, G0.8, and G0.5) were investigated. Dies of a mandibular first molar were made of composite resin. The zirconia crowns were luted with a resin composite cement (RelyX Unicem 2 Automix, 3M ESPE). Half of the specimens (n = 14 per group) were mouth-motion-fatigued (1.2 million cycles, 1.6 Hz, 200 N/ 5 - 55°C, groups named G2-F, G1.5-F, G1-F, G0.8-F, and G0.5-F). Single-load to failure was performed using a universal testing-machine. Fracture modes were analyzed. Data were statistically analyzed using a Weibull 2-parameter distribution (90% CI) to determine the characteristic strength and Weibull modulus differences among the groups. RESULTS. Three crowns (21%) of G0.8 and five crowns (36%) of G0.5 showed cracks after fatigue. Characteristic strength was the highest for G2, followed by G1.5. Intermediate values were observed for G1 and G1-F, followed by significantly lower values for G0.8, G0.8-F, and G0.5, and the lowest for G0.5-F. Weibull modulus was the lowest for G0.8, intermediate for G0.8-F and G0.5, and significantly higher for the remaining groups. Fatigue only affected G0.5-F. CONCLUSION. Reduced crown thickness lead to reduced characteristic strength, even under failure loads that exceed physiological chewing forces. Fatigue significantly reduced the failure load of 0.5 mm monolithic 3Y-TZP crowns.