Four-Week Repeated Intravenous Dose Toxicity and Toxicokinetic Study of TS-DP2, a Novel Human Granulocyte Colony Stimulating Factor in Rats

Lee, JooBuom,Lee, Kyungsun,Choe, Keunbum,Jung, Hyunseob,Cho, Hyunseok,Choi, Kiseok,Kim, Taegon,Kim, Seojin,Lee, Hyeong-Seok,Cha, Mi-Jin,Song, Si-Whan,Lee, Chul Kyu,Chun, Gie-Taek Korean Society of ToxicologyKorea Environmental Mu 2015 Toxicological Research Vol.31 No.4

TS-DP2 is a recombinant human granulocyte colony stimulating factor (rhG-CSF) manufactured by TS Corporation. We conducted a four-week study of TS-DP2 (test article) in repeated intravenous doses in male and female Sprague-Dawley (SD) rats. Lenograstim was used as a reference article and was administered intravenously at a dose of $1000{\mu}g/kg/day$. Rats received TS-DP2 intravenously at doses of 250, 500, and $1000{\mu}g/kg/day$ once daily for 4 weeks, and evaluated following a 2-week recovery period. Edema in the hind limbs and loss of mean body weight and body weight gain were observed in both the highest dose group of TS-DP2 and the lenograstim group in male rats. Fibro-osseous lesions were observed in the lenograstim group in both sexes, and at all groups of TS-DP2 in males, and at doses of TS-DP2 $500{\mu}g/kg/day$ and higher in females. The lesion was considered a toxicological change. Therefore, bone is the primary toxicological target of TS-DP2. The lowest observed adverse effect level (LOAEL) in males was $250{\mu}g/kg/day$, and no observed adverse effect level (NOAEL) in females was $250{\mu}g/kg/day$ in this study. In the toxicokinetic study, the serum concentrations of G-CSF were maintained until 8 hr after administration. The systemic exposures ($AUC_{0-24h}$ and $C_0$) were not markedly different between male and female rats, between the administration periods, or between TS-DP2 and lenograstim. In conclusion, TS-DP2 shows toxicological similarity to lenograstim over 4-weeks of repeated doses in rats.

Four-Week Repeated Intravenous Dose Toxicity and Toxicokinetic Study of TS-DP2, a Novel Human Granulocyte Colony Stimulating Factor in Rats

JooBuom Lee,Kyungsun Lee,Keunbum Choe,Hyunseob Jung,Hyunseok Cho,Kiseok Choi,Taegon Kim,Seojin Kim,Hyeong-Seok Lee,Mi-Jin Cha,Si-Whan Song,Chul Kyu Lee,Gie-Taek Chun 한국독성학회 2015 Toxicological Research Vol.31 No.4

TS-DP2 is a recombinant human granulocyte colony stimulating factor (rhG-CSF) manufactured by TS Corporation. We conducted a four-week study of TS-DP2 (test article) in repeated intravenous doses in male and female Sprague-Dawley (SD) rats. Lenograstim was used as a reference article and was administered intravenously at a dose of 1000 μg/kg/day. Rats received TS-DP2 intravenously at doses of 250, 500, and 1000 μg/kg/day once daily for 4 weeks, and evaluated following a 2-week recovery period. Edema in the hind limbs and loss of mean body weight and body weight gain were observed in both the highest dose group of TS-DP2 and the lenograstim group in male rats. Fibro-osseous lesions were observed in the lenograstim group in both sexes, and at all groups of TS-DP2 in males, and at doses of TS-DP2 500 μg/kg/day and higher in females. The lesion was considered a toxicological change. Therefore, bone is the primary toxicological target of TS-DP2. The lowest observed adverse effect level (LOAEL) in males was 250 μg/kg/day, and no observed adverse effect level (NOAEL) in females was 250 μg/kg/day in this study. In the toxicokinetic study, the serum concentrations of G-CSF were maintained until 8 hr after administration. The systemic exposures (AUC0-24h and C0) were not markedly different between male and female rats, between the administration periods, or between TS-DP2 and lenograstim. In conclusion, TS-DP2 shows toxicological similarity to lenograstim over 4-weeks of repeated doses in rats.

초등 5·6학년군 보건교과 내용요소 적절성 연구

조현아 ( Cho Hyuna ),송정혜 ( Song Jeunghye ),김경란 ( Kim Gyoungran ),박세은 ( Park Seeun ),심상희 ( Sim Sanghee ),이경순 ( Lee Kyungsun ),이은숙 ( Lee Eunsook ),이정희 ( Lee Junghee ),이지은 ( Lee Jieun ),이지혜 ( Lee Jihye ),이혜 한국보건교육학회 2020 한국보건교육학회지 Vol.6 No.1

본 논문은 초등학교 5·6학년 보건교과 내용요소 적절성 연구로서, 교과서와 장학자료 분석을 통해 보건교과 영역별 내용요소를 추출하고, 추출한 내용요소가 초등학교 5·6학년 군 보건교과 내용요소로서 적절한지 학생, 학부모, 교사를 대상으로 설문조사하여 집단 간 이해차이를 분석하였다. 우선 초등학교 5·6학년군 보건교과 내용체계를 4개 영역, 20개 핵심개념, 64개 내용요소로 제안하였다. 4개 영역은 ‘건강의 이해와 질병’, ‘생활 속의 건강한 선택’, ‘안전과 응급처치’, ‘건강자원과 사회문화’이다. ‘건강의 이해와 질병’ 영역은 3개의 핵심개념과 8개의 내용요소, ‘생활 속의 건강한 선택’은 3개의 하위 영역, ‘약물·담배·술’, ‘성건강’, ‘정서·정신건강’과 13개의 핵심개념, 39개의 내용요소, ‘안전과 응급처치’는 2개의 핵심개념과 8개의 내용요소, ‘건강자원과 사회문화’는 2개의 핵심개념과 9개의 내용요소로 구성하였다. 본 연구에서 추출한 보건교과 내용요소에 대해 학생, 학부모, 교사집단 모두 초등학교 5·6학년 보건교과 내용요소로서 적절하다고 응답하였다. 각 내용요소별 평균값은 4.11~4.69의 분포를 보였다. 각 영역별 평균값은 ‘건강의 이해와 질병’이 4.22~4.55, ‘생활 속의 건강한 선택’이 4.11~4.62, ‘안전과 응급처치’가 4.40~4.69, ‘건강자원과 사회문화’가 4.22~4.46이었다. 각 영역별 집단 간 평균값의 차이를 보면, ‘건강의 이해와 질병’ 영역, ‘약물·담배·술’ 영역, ‘안전과 응급처치’ 영역은 교사 집단이, ‘성건강’ 영역은 교사와 학부모 집단이, ‘건강자원과 사회문화’ 영역은 학생 집단의 평균값이 대체로 높았고, ‘정서·정신건강’ 영역은 집단 간 유의미한 차이가 없었다. 2015개정교육과정이 전면 적용되는 현재, 교사의 교육과정 문해력과 교육과정 재구성 역량이 강조되나, 국가수준에서 제시된 초등학교 보건교과 교육과정이 없다는 것은 실제보건교육이 초등학교 현장에서 실시되고 있음에도 무엇을, 어떻게 되도록 가르쳐야 하는지에 대한 기준점이 없음을 의미한다. 비록 교사별 교육과정이 강조되고, 교사가 교육과정 전문가임에 틀림없으나, 국가수준의 교육과정이 제시되어야 교육과정 안에서 교사는 자신의 전문성을 발휘할 수 있을 것이다. 그러므로 초등학교 보건교과 교육과정의 마련이 시급하다. This paper is a study on the appropriateness of the content element of the health subject in the 5th and 6th grades of elementary school. This, through the analysis of textbooks and scholarship materials, extracted the content elements according to the health subject areas, and then surveyed the questionaire on the samples of students, parents, and teachers to see if the content element of the health subject in the 5th and 6th grades of elementary school is appropriate, finally analyzing the understanding difference of the groups. First of all, this divided the content element of the health subject in the 5th and 6th grades of elementary school into four areas, 20 key concepts, and 64 content elements for the convenience of analysis. The four areas are "Health Understanding and Disease," "Healthy Choice in Life," "Safety and First Aid" and "Health Resources and Social Culture." “Health Understanding and Disease" area has three key concepts and eight content elements. “Healthy Choice in Life" has three sub-area areas; "Drugs, Tobacco and Alcohol". "Health and Mental Health" and 13 key concepts; "Safety and First Aid" has two key concepts and eight key content elements; "Health Resources and Social Culture" has two key concepts and nine content elements. All the sample groups responded the health subject content elements extracted from this study to be appropriate as those for the 5th and 6th grades of elementary school. The average value of every content element was distributed at 4.11 to 4.69. The average values of every area are following: “Health Understanding and Disease”, 4.22-4.55; “Healthy Choices in Life”, 4.11-4.62; “Safety and First Aid”, 4.40-4.69; “Health Resources and Social Culture”, 4.22-4.46. Observing the differences in average values between groups of different areas, the average values of the areas of "Health Understanding and Disease," "Drugs, Tobacco and Alcohol," and "Safety and First Aid" are high in the group of teachers, those of the area of “Sexual Health” are high in the groups of teachers and parents, those of the areas of "Health Resources and Social Culture" are generally high in the group of students, and those of the area of "Sentimental and Mental Health" have no significant difference between groups. At present situation under the comprehensive application of 2015 revised curriculum, although teachers' literacy on curricula and ability to reconstruct curricula are emphasized, the fact that there is no curriculum for the health subject of elementary school suggested at the national level actually means that there is no criterion on what and how to teach it in spite of teaching the health education in the elementary school scene. It is sure that every teacher's curriculum should be emphasized and the teacher must be an expert in the curriculum, but, nevertheless, the national level curriculum have to be presented so that the teacher can display his or her expertise within the curriculum. Therefore, it is urgent to prepare a health subject curriculum for elementary school.

Fluorescent Blue Materials for Efficient Organic Light-Emitting Diode with High Color Purity

Kyungsun Choi,Chanhyo Lee,Kwan Hee Lee,Su Jin Park,손성욱,정영근,홍종인 대한화학회 2006 Bulletin of the Korean Chemical Society Vol.27 No.10

We report a new series of blue dopants composed of both electron donating and electron accepting moieties in one molecule, based on nalidixic acid. The EL device derived from the dopant exhibits pure blue light emission (0.15, 0.14) The current efficiency is estimated to be 3.88 cd/A at 100 cd/m2, which shows remarkable enhancement, compared to that of the host itself (2.5 cd/A at 100 cd/m2) under the same conditions. These results demonstrate that the incorporation of a proper guest into the host in a guest-host doped system improves not only the purity of the fluorescent blue emission but also elevates its quantum efficiency, thus improving the OLED performance.

Fabrication of Humidity Control Ceramics from Drinking-Water Treatment Sludge and Onggi Soil

Lee, Min-Jin,Lee, Hyeon-Jun,Kim, Kyungsun,Hwang, Hae-Jin The Korean Ceramic Society 2016 한국세라믹학회지 Vol.53 No.3

In this study, humidity control ceramics with good adsorption/desorption capabilities and high strength were fabricated from drinking-water treatment sludge (DWTS) and Onggi soil. The DWTS powder heat-treated at $800^{\circ}C$ and Onggi soil were mixed at weight ratios of 40:60, 50:50, 60:40, and 70:30 and fired at $800-1000^{\circ}C$. With increasing DWTS content, density and flexural strength increased. For the sample with a DWTS:Onggi soil weight ratio of 70:30, porosity and specific surface area decreased with increasing firing temperature, attributed to densification and grain growth at high firing temperatures. From the results obtained, a firing temperature of $800^{\circ}C$ is the optimum condition for fabricating humidity control ceramics with good adsorption/desorption capabilities and strength. The maximum adsorbed amount for the sample fired at $800^{\circ}C$ was $439g/m^2$.

Preparation and Optical and Electronic Applications of Functional Organic-Inorganic Hybrid Materials

Kyungsun LEE,Seowon CHOI,Daehyeon HWANG,Kyungbok EO,Myoeum KIM,Yong Ku KWON 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

와송추출물의 비만유발 ICR 마우스에서의 항비만 효과

이경선 ( Kyungsun Lee ),이형선 ( Hyeong-seon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 한국미생물·생명공학회지 Vol.45 No.4

본 연구에서는 와송로부터 추출한 열수 추출물의 항비만 효과를 알아보기 위해 4주령의 수컷 쥐를 1주일간 적응시킨 후, 8주간 정상식이군을 제외한 모든 군에 고지방식이를 제공하여 비만을 유도하였다. 그 후 정상식이군, 고지방식이군, 2.5% 와송추출물 식이군, 5% 와송추출물 식이군으로 나누어 6주간 식이 투여를 실시하였다. 체중증가량은 고지방식 이군에서 유의적으로 증가하였으며, 와송추출물 첨가 식이군에서 유의적으로 감소하였다. 이러한 결과는 식이섭취량에서 군간의 유의적 차이가 없었음에도 불구하고 체중증가량과 식이효율비가 와송추출물 첨가 식이군에서 탁월한 감소 효과를 보였다. 고지방 식이에 의한 혈청 중 총콜레스테롤, 중성지방, LDL-콜레스테롤의 증가는 와송추출물의 섭취로 인해 낮아졌으며, HDL-콜레스테롤의 현저한 증가를 가져왔다. 부고환 지방조직에서 관찰한 지방세포의 크기 또한 와송추출물 섭취군에서 정상군과 매우 유사한 형태를 보여준다. 위의 실험 결과를 종합할 때, 고지방 식이와 함께 공급한 와송추출물은 체중감소 및 체지방 축척의 억제와 더불어 혈중 지방 수준의 개선으로 다양한 비만 유래 질병에 대한 긍정적 영향을 미치는 것으로 판단된다. We investigated the anti-obesity effects of the water extract from Orostachys japonicus in high-fat-fed mice. For the analysis, 24 mice were divided into four groups: normal (N) group, high-fat diet (HFD) group, high-fat diet supplemented with 2.5% O. japonicus (HFD-OJL) group, and high-fat diet supplemented with 5% O. japonicus (HFD-OJH) group. The body weight gain and food efficiency ratio increased with a high-fat diet, but significantly decreased in the HFD-OJ groups compared with those in the HFD group. Levels of serum triglyceride, total cholesterol, and LDL-cholesterol were significantly lower, but the HDL-cholesterol level was significantly higher in the HFD-OJH group. The adipocytes within the epididymal adipose tissue were found to have expanded in the HFD group. However, the adipocytes in the HFD-OJ groups were compara-ble to those of the N group. These results suggest that O. japonicus has potential as an anti-obesity agent by decreasing body weight and lowering the serum lipid levels in obese mice.

발달장애학생 부모의 진로지원행동 목록 개발 및 타당화

이경선(Lee Kyungsun),이미숙(Lee Misuk) 한국장애인고용공단 고용개발원 2019 장애와 고용 Vol.29 No.2

Purpose: The purpose of this study is to develop a inventory of parents who have developmental disabilities to support their child's career. This inventory can help students with developmental disabilities to maintain and generalize their occupational skills learned at home and at school. The study is to develop a specific inventory of parents to support the career related behavior of students with developmental disabilities. As well, then validated the validity and reliability of the inventory. Method: This study developed a career support behavior inventory for parents of students with developmental disabilities using exploratory factor analysis and confirmatory factor nalysis. Results: The career support inventory for parents of students with developmental disabilities six factors and 31 inventories were developed. This inventory is based on 7 items for ‘the information career support’ factor, 7 items for ‘the independent career support’ factor, 6 items for ‘the psychological career support’ factor, 4 items for ‘the expert utilization support' factor, 'the social participation support' factor, and 3 items for 'the home life management support' factor. Implications: In subsequent studies, there is a need to develop a career education program for parents of children with developmental disabilities. In addition, it is necessary to explore the improvement points and support measures through analysis of career support cases that parents of developmentally disabled students have performed for their children. 연구목적: 이 연구는 발달장애학생의 부모가 자녀의 진로 관련 행동을 지원할 수 있도록 구체화된 목록을 개발하고, 목록의 타당도와 신뢰도를 검증하는 연구이다. 연구방법: 이 연구에서는 탐색적 요인분석과 확인적 요인분석을 통해 발달장애학생 부모의 진로지원행동 목록을 개발하였다. 연구결과: 발달장애 학생의 부모의 진로지원행동 목록은 6개 요인과 31개 항목으로 개발되었다. 즉, ‘정보적 진로지원’영역 7개, ‘자립적 진로지원’영역 7개, ‘심리적 진로지원’영역 6개, ‘전문가 활용지원’영역 4개, ‘지역사회 참여지원’영역 4개, ‘가정생활 관리지원’영역 3개로 구성되었다. 시사점: 후속 연구에서는 발달장애학생 부모를 위한 진로교육 프로그램을 개발할 필요가 있으며, 발달장애학생 부모가 자녀에게 수행한 진로지원 사례분석을 통해 개선점 및 지원방안을 탐색할 필요가 있다.

서울시 NPO 지원센터

이경선(Lee Kyungsun),이윤하(Lee Yoonha) 현대건축사 2014 (월간)Maru Vol.- No.151

Yes or No

이경선(Kyungsun Lee) 한국디자인학회 2015 한국디자인학회 국제초대전도록 Vol.2015 No.10