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RISS 인기검색어
- 검색키워드
- 저자 : Kyungpyo Nam (검색결과 4 건)
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Nam, Hyun,Kim, Ji-Hye,Hwang, Ji-Yoon,Kim, Gee-Hye,Kim, Jae-Won,Jang, Mi,Lee, Jong-Ho,Park, Kyungpyo,Lee, Gene Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.6
Patients with head and neck cancer are treated with therapeutic irradiation, which can result in irreversible salivary gland dysfunction. Because there is no complete cure for such patients, stem cell therapy is an emerging alternative for functional restoration of salivary glands. In this study, we investigated in vitro characteristics of primarily isolated epithelial cells from human salivary gland (Epi-SGs) and in vivo formation of acini-like structures by Epi-SGs. Primarily isolated Epi-SGs showed typical epithelial cell-like morphology and expressed E-cadherin but not N-cadherin. Epi-SGs expressed epithelial stem cell (EpiSC) and embryonic stem cell (ESC) markers. During long-term culture, the expression of EpiSC and ESC markers was highly detected and maintained within the core population with small size and low cytoplasmic complexity. The core population expressed cytokeratin 7 and cytokeratin 14, known as duct markers indicating that Epi-SGs might be originated from the duct. When Epi-SGs were transplanted in vivo with Matrigel, acini-like structures were readily formed at 4 days after transplantation and they were maintained at 7 days after transplantation. Taken together, our data suggested that Epi-SGs might contain stem cells which were positive for EpiSC and ESC markers, and Epi-SGs might contribute to the regeneration of acini-like structures in vivo. We expect that Epi-SGs will be useful source for the functional restoration of damaged salivary gland.
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Park, Kyungpyo,Kim, Seong-Ah,Nam, Sang Chae,Kim, Joong-Soo,Lee, Jong-Heun Korean Academy of Oral Biology and the UCLA Dental 2000 International Journal of Oral Biology Vol.25 No.3
Medually dorsal horn〔MDH〕neurons, known as the sensory neurons, are involved in orofacial pain transmission. In the present study, we investigated the types of neurotransmitters that affect intracellular free calcium concentration〔[Ca^2+]_i〕, and which modulate pain sensation in the trigeminal sensory pathway. [Ca^2+]_i was measured in fura-2 acetoxymethyl eser (fura-2) loaded MDH neurons in neonatal rat pups by microspectrofluorimetry. Most of the neurons〔97.7%〕responded to L-glutamate by increasing [Ca^2+]_i and this response was reversible and consistent. [Ca^2+]_i increase was dose dependent, i.e., 0.24±0.06〔ΔF_340/F_380 ratio, n=2〕, 1.77±0.35 (n=10) and 1.82±0.15(n=2), at 30μM, 300μM and 1mM of glutamate, respectively. Substance P〔SP, 1nM〕also evoked a [Ca^2+]_i increase〔1.6±0.62, n=2〕in MDH neurons; however, this occurred in only 18.2% of neurons tested. In contrast to glutamate, the SP receptor showed a rapid desensitization. N-methyl-D-aspartate〔NMDA〕also increased [Ca^2+]_i in 21.1% of the neurons tested. NMDA-induced [Ca^2+]_i increase was not observed in the absence of extracellular Ca^2+. The coexpression of the SP and NMDA receptors with the glutamate receptor or the SP receptor with the NMDA receptor was observed in these neurons, although both expression percentages were low. In conclusion, L-glutamate, SP and NMDA functional receptors are expressed early in neonatal MDH neurons and may act as excitatory modulators in MDH neurons by increasing [Ca^2+]_i.
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Retrograde Intramedullary Nailing for Distal Femur Fracture with Osteoporosis
Jihyeung Kim,강승백,Kyungpyo Nam,Seung Hwan Rhee,Jong Won Won,Hyuk-Soo Han 대한정형외과학회 2012 Clinics in Orthopedic Surgery Vol.4 No.4
Background: The incidence of distal femur fracture in the elderly has been increasing recently, and commonly occurs with osteoporosis. Retrograde intramedullary nailing has been considered a good surgical option for distal femur fracture. The purpose of the present study was to present our surgical results with retrograde intramedullary nailing for distal femur fractures with osteoporosis. Methods: Thirteen patients diagnosed with extra-articular distal femur fracture and osteoporosis and managed with retrograde intramedullary nailing were retrospectively reviewed. Cement augmentation was used in four patients, shape memory alloy was used in eight patients and both were used in one patient. All patients were followed up for more than 2 years. Radiologic alignments were scored and Tegner and the Lysholm activity score was used for a functional assessment. Results: The average time to clinical union was 13 weeks (range, 10 to 15 weeks). In 12 of our cases, the total alignment scores were excellent. At the last follow-up, the mean range of motion was 116° (range, 110° to 125°). The average functional score at postoperative 1 year was 2.6 (range, 1 to 5). Conclusions: Retrograde intramedullary nailing is a good surgical option for distal femur fracture with osteoporosis. Cement augmentation and shape memory alloy can also be used for added mechanical stability. This surgical technique is very useful for distal femur fracture with osteoporosis as it promotes fracture healing and early rehabilitation.
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Oh, Seog Bae,Yoon, Chan Song,Nam, Sang Chae,Park, Kyungpyo,Lee, Jong-Heun,Kim, Joong-Soo Korean Academy of Oral Biology and the UCLA Dental 1997 International Journal of Oral Biology Vol.22 No.4
Capsaicin produces initial irritation followed by insensitivity to noxious stimuli. It can not be easily used in clinical pain control due to its toxic effects. Recently, a capsaicin derivative (KR) was developed to use as an analgesic with fewer toxic effects. However, the mechanism of action and clinical effects of KR are not well understood. In this experiment we tried to explore the cellular mechanisms of the analgesic effect induced by capsaicin and KR in DRG or TG neurons. The DRG and TG neurons were acutely isolated into single neuronal cells from neonatal or adult rats with trypsin treatment and mechanical trituration. Voltage dependent calcium currents were measured with whole-cell patch clamp configuration in small diameter sensory neurons (between 10 μm and 20μm). On the basis of activation threshold, Ca^2+ currents of DRG and TG neurons were classified as LVA (T-type) and HVA I_ca. HVA I_ca were subclassified into L-type and N-type. The amplitude of L-type calcium current was remarkably inhibited by application of 1 μM KR in DRG. The inhibition of calcium current recovered significantly at five minutes after application of KR. In most capsaicin-sensitive neurons in TG, capsaicin irreversibly inhibited voltage dependent calcium currents. Capsaicin and KR-induced inhibition of voltage dependent calcium currents would be expected to reduce neurotransmitter release from both central and peripheral terminals of the sensitive primary sensory neurons. This can be proposed as one possible mechanism for the analgesic and anti-inflammatory effects induced by them.
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