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Kyungae Jo,Ki-Bae Hong,Hyung Joo Suh 한국식품영양과학회 2020 Preventive Nutrition and Food Science Vol.25 No.1
In this study, we used various proteinases to investigate the effect of whey protein hydrolysates on proliferation and differentiation of MC3T3-E1 osteoblasts. To confirm hydrolysis of the whey protein hydrolysates, the yield and α-amino acid content were determined. Since osteogenic cell activity is an important factor in osteogenesis, we evaluated the proliferation of osteogenic cells by measuring 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and alkaline phosphatase (ALP) activity. To analyze bone matrix formation, we identified calcium deposition by staining with Alizaline red-S. The free amino acid content was significantly higher in the whey protein hydrolysates prepared using Protamex, Flavourzyme, and Alcalase than in the control. When cells were treated with 500㎍/mL of whey protein hydrolysates prepared using Protamex and Alcalase, cell proliferation increased by 120% and 130%, respectively, compared with the control group. In addition, ALP activity was significantly higher following treatment with 500㎍/mL of whey protein hydrolysates prepared using Protamex and Alcalase (142.61% and 135.06%, respectively; P<0.05). Furthermore, when treated with 125㎍/mL of the same hydrolysates, the rate of calcium deposition increased significantly to 157.56% compared with the control group (P<0.05). Therefore, our results suggest that whey protein hydrolysates prepared using Protamex and Alcalase may have more beneficial effects on osteoblast proliferation and bone health than those prepared using other proteolytic enzymes.
Kyungae Jo,SangDuk Jeon,Chang-Won Ahn,Sung Hee Han,Hyung Joo Suh 한국식품영양과학회 2017 Preventive Nutrition and Food Science Vol.22 No.4
We evaluated the sleep enhancement activity of the medicinal herbs valerian (Valeriana officinalis), jujube (Ziziphus jujube), lotus seed (Nelumbo nucifera), Gastrodia elata, Polygonatum sibiricum, and baekbokryung (Poria cocos), which can relieve insomnia in a Drosophila model. Locomotor activity was measured in the Drosophila model to evaluate the sleep activity of Korean medicinal herbs traditionally used as sleep aids. The group treated with lotus seed extract showed less nocturnal activity. Treatment with 10 or 20 mg/mL of P. sibiricum significantly reduced nocturnal activity compared to the control group (P<0.05). The activity and sleep bouts of fruit flies were significantly decreased by a high-dose treatment (10 mg/mL) of lotus or P. sibiricum extracts at night. Caffeine-treated Drosophila showed increased nocturnal activity and decreased total sleep time (P<0.05). Flies receiving the 10 mg-doses of lotus seed or P. sibiricum extract showed significantly different nocturnal locomotor activity and total sleep time compared to caffeine-treated Drosophila. Lotus seed and P. sibiricum extracts are attractive and valuable sleep-potentiating nutraceuticals.
Kyungae Jo,Woo Young Jang,Beom Sik Yun,Jin Soo Kim,Hyun-Sun Lee,Yeok Boo Chang,Hyung Joo Suh 한국축산식품학회 2021 한국축산식품학회지 Vol.41 No.4
The effect of deer antler extract on muscle differentiation and muscle atrophy were evaluated to minimize muscle loss following aging. Various deer antler extracts (HWE, hot water extract of deer antler; FE, HWE of fermented deer antler; ET, enzyme-assisted extract of deer antler; UE, extract prepared by ultrasonication of deer antler) were evaluated for their effect on muscle differentiation and inhibition of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR)-induced muscle atrophy in C2C12 cells. Morphological changes according to the effect of antler extracts on muscle differentiation were confirmed by Jenner-Giemsa staining. In addition, the expression levels of genes related to muscle differentiation and atrophy were confirmed through qRT-PCR. In the presence of antler extracts, the length and thickness of myotubes and myogenin differentiation 1 (MyoD1) and myogenic factor 5 (Myf5) gene expression were increased compared to those in the control group (CON). Gene expression of AMP-activated protein kinase (AMPK), MyoD1, and myogenin, along with the muscle atrophy factors muscle RING finger-1 (MuRF-1) and forkhead box O3a (FoxO3a) upon addition of deer antler extracts to muscle-atrophied C2C12 cells was determined by qRT-PCR after treatment with AICAR. The expression of MuRF-1 and FoxO3a decreased in the groups treated with antler extracts compared to that in the group treated with AICAR alone. In addition, gene expression of MyoD1 and myogenin in the muscle atrophy cell model was significantly increased compared that into the CON. Therefore, our findings indicate that antler extract can increase the expression of MyoD1, Myf5 and myogenin, inhibit muscle atrophy, and promote muscle differentiation.
Kyungae Kim,MiRan Bang 한국간호과학회 2021 한국간호과학회 학술대회 Vol.2021 No.10
Aim(s): It is important to prevent the increase in the prevalence of diabetes mellitus (DM) worldwide by efficiently managing its controllable risk factors. This study aimed to identify factors associated with the 4-year estimated incidence of DM by gender and provide basic data for a gender-specific strategic approach to lifestyle modification. Method(s): In this study, we carried out a secondary data analysis using raw data from the seventh Korea National Health and Nutrition Examination Survey (KNHANES, 2016–2018). The KNHANES is a descriptive correlational survey designed to examine gender differences in the factors associated with the 4-year estimated incidence of DM. This study included 9,614 Korean adults (4,134 men and 5,480 women) aged 40-69 years without a diagnosis of DM. For statistical analysis, complex sample analysis was performed for gender comparison using χ2-test or one-way analysis of variance; multiple regression analysis was performed to analyze the gender-specific influencing variables of 4-year estimated DM incidence. Result(s): The waist-to-height ratio, an indicator of central obesity in adults, had the strongest association with the 4-year estimated incidence of DM in both groups (M: β=0.33, p≤0.001; F: β=0.38, p≤0.001). The influencing variables were monthly drinking rate (β=0.07, p≤0.001) and sleep time (β=-0.03, p<0.05) in men, and sedentary time in women (β=0.03, p<0.05). The overall explanatory power of these variables was 11.3% for men and 14.3% for women. Thus, significant gender differences were found in the 4-year estimated incidence of DM. Conclusion(s): Therefore, intervention programs need to be gender-specific to enhance the efficacy of the interventions in reducing the incidence of DM, and such intervention programs should be administered with a strategic approach differentiated by gender.