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감궁탕이 사람의 갑상선세포에서 Fas 매개성 apoptosis에 미치는 영향
남경수,손옥례,김미경,김철호,소명숙,전병훈,손윤희,Nam Kyung Soo,Son Ok Lye,Kim Mee Kyung,Kim Cheorl Ho,So Myung Suk,Jeon Byung Hun,Shon Yun Hee 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3
Inflammatory cytokine, abundantly produced in Hashimoto's thyroiditis, induced Fas expression in normal thyrocytes. We determined that susceptibility to Fas-activated apoptosis could be influenced by inflammatory cytokine and investigated a potential role of Gamgung-tang (GGT, Glycyrrhizae Radix, black beans, Angelicae Radix and Cnidii Rhizoma) in the thyroid follicular cells. $IL-1\beta$ was able to induce Fas expression in normal thyrocytes. GGT inhibited $IL-1\beta-induced$ Fas expression. Thyroid follicular cells were found to undergo DNA fragmentation with the inflammatory cytokines. GGT inhibited DNA fragmentation in a dose-dependent manner. These results suggest that GGT inhibit Fas-mediated apoptosis in thyroid follicular cells, therefore, may have therapeutic potential in the treatment of autoimmune chronic thyroiditis.
불가사리 단백추출물이 Cytochrome P450 1A1과 Ornithine Decarboxylase 활성에 미치는 영향
남경수,김미경,조현정,손윤희,Nam, Kyung-Soo,Kim, Mee-Kyung,Cho, Hyun-Jung,Shon, Yun-Hee 한국해양바이오학회 2006 한국해양바이오학회지 Vol.1 No.2
The effect of protein extract from Asterina pectinifera on proliferation of human breast cancer cells and activities of cytochrome P450 1A1 and ornithine decarboxylase was tested. Protein extract from Asterina pectinifera inhibited the growth of both estrogen-dependent MCF-7 and estrogen-independent MDA-MB-231 human breast cancer cells. Cytochrome P450 1A1 activity was significantly inhibited by the protein extract from Asterina pectinifera at concentrations of 80 (p<0.05), 120 (p<0.01) and $160{\mu}g/m{\ell}$ (p<0.01). The extract inhibited induction of ornithine decarboxylase by 12-O-tetradecanoylphorbol-13-acetate, a key enzyme of polyamine biosynthesis, which is enhanced in breast tumor promotion. Therefore, Asterina pectinifera is worth further investigation with respect to breast cancer chemoprevention or therapy. 별불가사리 단백추출물을 조제하여 유방암 유발억제효과를 측정한 결과, 별불가사리 단백추출물은 estrogen-의존성의 유방암세포(MCF-7)와 estrogen-비의존성 유방암세포(MDA-MB-231)의 증식을 농도의존적으로 억제하는 효과가 있었으며, 유방암발생 발암물질 활성에 관여하는 cytochrome P450 1A1 활성도 저해하였다. 또한 유방암발생의 촉진단계에 주요한 기능을 가진 ODC 활성도 억제하였으므로 별불가사리 단백추출물은 유방암 발생을 저해할 것으로 기대된다.
별불가사리 단백추출물이 유방암예방 및 전이억제 효소계에 미치는 영향
남경수,김미경,조현정,손윤희,Nam, Kyung-Soo,Kim, Mee-Kyung,Cho, Hyun-Jung,Shon, Yun-Hee 한국해양바이오학회 2006 한국해양바이오학회지 Vol.1 No.3
별불가사리 단백추출물을 조제하여 유방암 발생 및 전이억제효과를 측정한 결과 종양세포증식에 관여하는 aromatase 활성이 농도의존적으로 억제효과가 있었으며, 침윤성 유방암에서 과발현하는 COX-2 단백질 발현을 억제하였다. 그리고 유방암이 진행될수록 활성이 증가하는 MMP-9도 농도의존적 저해율을 보였다. 그러므로 별불가사리 단백추출물은 유방암억제기전에 관련된 더 많은 연구를 통하여 유방암 예방물질로서 개발 가능성이 있는 것으로 보인다. The effect of protein extract from Asterina pectinifera on breast cancer chemopreventive (aromatase and cyclooxygenase-2) and metastatic (matrix metalloproteinase) enzymes was tested. Protein extract from A. pectinifera was capable of suppressing aromatase in a human placenta microsomal assay. Cyclooxygenase-2 (COX-2) activity was significantly inhibited by the protein extract from A. pectinifera at concentrations of 10, 20 and $40{\mu}g/m{\ell}$. The extract markedly reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated matrix metalloproteinase (MMP)-9 activity. These results suggest that A. pectinifera could be of therapeutic value in preventing human breast cancer.
남경수 ( Kyung Soo Nam ),이미현 ( Mi Hyun Lee ),이규식 ( Kyu Shik Lee ),신진선 ( Jin Sun Shin ),정상빈 ( Sang Bin Jeong ) 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.1
We investigated the effect of low molecular weight chitosan oligosaccharide (COS-L, Mw < 1,000) on breast cancer development and progression through the measurement of quinone reductase (QR) activity and glutathione (GSH) content and the determination of ornithine decarboxylase activity (ODC) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). In a concentration range of 0.1 to 5 mg/mL, QR activity and GSH content in MCF-7 breast cancer cells were significantly increased maximal 76.5% and 56.3% by COS-L in a dose-dependent manner, respectively. Low molecular weight chitosan oligosaccharide effectively inhibited the activity of ODC, a key enzyme in cancer promotion. These results demonstrate that COS-L may prevent the development and promotion of breast cancer through the induction of QR activity, the increase of GSH content and the inhibition of ODC activity.
비장면역법으로 인지질에 대한 고효율의 단클론성항체 산생
남경수 ( Kyung Soo Nam ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.2
Monoclonal antibodies against phospholipids, especially bioactive phospholipids, give very important information to understand cellular signal transduction and receptor conformation. A series of monoclonal antibodies that recognize phosphatidylcholine, phosphatidylserine and phosphatidylinositol-4-monophosphate were established by intrasplenic immunization method. Originally, this technique was the method of choice for the immunization with very little amounts (about nanogram) of immunogen. However, using this method, I immunized phospholipids which have been known to have poor immunogenicity (hapten) due to their low molecular weight, and attained successful outcomes. Moreover, 8-mercaptoguanosine administration showed a considerable increase in hybridoma production and PC(phosphatidylcholine) specific monoclonal antibodies. In future, this technique may be helpful to produce monoclonal antibodies which have been difficult to make until now.
Anti-IL-1beta 단일클론 항체를 이용해서 발열환자의 뇨중 IL-1beta inhibitor의 확인
남경수(Kyung Soo Nam),배윤수(Yoon Soo Bae),남명수(Myoung Soo Nam),오은숙(Eun Sook Oh),박순희(Soon Hee Park),최인성(In Seong Choe),정태화(Tai Wha Chung) 대한약학회 1993 약학회지 Vol.37 No.4
To effectively purify of IL-1 inhibitor from human febrile urine, we have established monoclonal antibody that reacts with human recombinant interleukin 1beta(IL-1beta). The antibody, designated ON-1, was highly specific to IL-1beta and no cross-reaction with other cytokines(IL-1alpha and IL-4) was observed. As the results of ELISA inhibition assay and Western blotting method, it was further identified that ON-1 had high binding specificity with IL-1beta. IL-1 receptor binding material from febrile patient urine was effectively purified with affinitv column chromatography which conjugated with ON-1. This urinary material inhibited the thymocyte proliferation in a dose-dependent manner. IL-1beta induced thymocyte proliferation activity was inhibited to 67.3% at 6mcg of the purified urinary material. The result may suggest that this urinary material the purified urinary material. The result may suggest that this urinary material will have antagonic effect on IL-1 action mechanism and act IL-1beta inhibitor.
남경수(Kyung Soo Nam) 한국키틴키토산학회 2001 한국키틴키토산학회지 Vol.6 No.2
N/A 2,3,7,8-tetrachlorodibenzo-f-dioxin (TCDB ) is one of the most potent toxins and tumor promoters known to man . Chitosan is a modified, natural carbohydrate polymer derived by deacetylation of chitin, a major component of the shells of crustacea such as crab, shrimp and crawfish, and the second most abundant natural biopolymer. This study was investigated to examine the induction of glutathione S-transferase (GST), glutathione peroxidase (GSH-Px) and glutathione (GSH) levels by 2,3,7,8-TCDD and the ability of chitosan oligosaccharide I (1,000<Mw<3,000, COS I) and chitosan oligosaccharides Ⅱ (3,000<Mw<5,000, COS Ⅱ) to prevent the hepatotoxicity of 2,3,7,8-TCDD. The animals received 2,3,7,8-TCDD (25 yg/kg body wt) once orally 7days prior to sacrifice 2,3,7,8-TCDB administration resulted in a 44.4% decrease in GSH-Px activity. COS I and COS Ⅱ treatment prevented the effect of 2,3,7,8-TCDD-induced alteration of GSH-Px activity. GSH level was increased with 2,3,7,8-TCDD, and COS I and COS Ⅱ did not affect 2,3,7,8-TCDD-induced alterations significantly. Mice treated with COS Ⅱ increased GST activity. These results suggest that chitosan oligosaccharides may prevent the toxic manifestations of 2, 3, 7, 8-TCDD mediated hepato toxicity.
키토산올리고당이 소 미성숙난포란의 핵성숙 및 수정에 미치는 영향
남경수(Kyung Soo Nam) 한국키틴키토산학회 1999 한국키틴키토산학회지 Vol.4 No.2
N/A This study was performed to evaluate the acute oral toxicity of chitooligosaccharides in Sprague-Dawley(SD) rats. Chitosan and chitooligosaccharides were administered at dose levels of 5,000, 2,500, 1,250, 625, 312.5 and 0 mg/kg body weight. After single oral administration to both sexes of SD rats, we observed rats for 14 days. All chitooligosaccharides did not induce any toxic signs in mortalities, clinical findings, body weights and gross findings of the rats. In view of result, it was impossible to estimate LD50 values in SD rats. Therefore, it was concluded that chitooligosaccharides have no effect on acute toxicity and side effect in rats.