Poster Session 4 : Oncogenes and Tumor Suppressor Genes Virology Immunology Tumor Biology Developmental Biology Plant Biology Molecular Medicine & Gene Therapy Procaryotic Genetics Biotechnology ; Identification of DC21 as a Novel Target Gene Counter-regu

( Kyoung Ah Kong ),( Ji Young Jang ),( Choong Eun Lee ) 한국생화학분자생물학회 (구 한국생화학회) 2002 추계학술대회집 Vol.2002 No.-

Identification of DC21 as a Novel Target Gene Counter-regulated by IL-12 and IL-4

Kong, Kyoung-Ah,Jang, Ji-Young,Lee, Choong-Eun 생화학분자생물학회 2002 Journal of biochemistry and molecular biology Vol.35 No.6

The Th1 vs. Th2 balance is critical for the maintenance of immune homeostasis. Therefore, the genes that are selectively-regulated by the Th1 and Th2 cytokines are likely to play an important role in the Th1 and Th2 immune responses. In order to search for and identify the novel target genes that are differentially regulated by the Th1/Th2 cytokines, the human PBMC mRNAs differentially expressed upon the stimulation with IL-4 or IL-12, were screened by employing the differential display-polymerase chain reaction. Among a number of clones selected, DC21 was identified as a novel target gene that is regulated by IL-4 and IL-12. The DC21 gene expression was up-regulated either by IL-4 or IL-12, yet counter-regulated by co-treatment with IL-4 and IL-12. DC21 is a dendritic cell protein with an unknown function. The sequence analysis and conserved-domain search revealed that it has two AU-rich motifs in the 3'UTR, which is a target site for the regulation of mRNA stability by cytokines, and that it belongs to the N-acetyltransferase family. The induction of DC21 by IL-12 peaked around 8-12 h, and lasted until 24 h. LY294002 and SB203580 significantly suppressed the IL-12-induced DC21 gene expression, which implies that PI3K and p38/JNK are involved in the IL-12 signal transduction pathway that leads to the DC21 expression. Furthermore, tissue blot data indicated that DC21 is highly expressed in tissues with specialized-resident macrophages, such as the lung, liver, kidney, and placenta. Together, these data suggest a possible role for DC21 in the differentiation and maturation of dendritic cells regulated by IL-4 and IL-12.

Identification of DC21 as a Novel Target Gene Counter-regulated by IL-12 and IL-4

( Kyoung Ah Kong ),( Ji Young Jang ),( Choong Eun Lee ) 생화학분자생물학회 2002 BMB Reports Vol.35 No.6

The Th1 vs. Th2 balance is critical for the maintenance of immune homeostasis. Therefore, the genes that are selectively-regulated by the Th1 and Th2 cytokines are likely to play an important role in the Th1 and Th2 immune responses. In order to search for and identify the novel target genes that are differentially regulated by the Th1/Th2 cytokines, the human PBMC mRNAs differentially expressed upon the stimulation with IL-4 or IL-12, were screened by employing the differential displaypolymerise chain reaction. Among a number of clones selected, DC21 was identified as a novel target gene that is regulated by IL-4 and IL-12. The DC21 gene expression was up-regulated either by IL-4 or IL-12, yet counterregulated by co-treatment with IL-4 and IL-12. DC21 is a dendritic cell protein with an unknown function. The sequence analysis and conserved-domain search revealed that it has two AU-rich motifs in the 3`UTR, which is a target site for the regulation of mRNA stability by cytokines, and that it belongs to the N-acetyltransferase family. The induction of DC21 by IL-12 peaked around 8-12h, and lasted until 24h. LY294002 and SB203580 significantly suppressed the IL-12-induced DC21 gene expression, which implies that PI3K and p38/JNK are involved in the IL-12 signal transduction pathway that leads to the DC21 expression. Furthermore, tissue blot data indicated that DC21 is highly expressed in tissues with specialized-resident macrophages, such as the lung, liver, kidney, and placenta. Together, these data suggest a possible role for DC21 in the differentiation and maturation of dendritic cells regulated by IL-4 and IL-12.

Comparison of Prevalence- and Smoking Impact Ratio-Based Methods of Estimating Smoking-Attributable Fractions of Deaths

Kong, Kyoung Ae,Jung-Choi, Kyung-Hee,Lim, Dohee,Lee, Hye Ah,Lee, Won Kyung,Baik, Sun Jung,Park, Su Hyun,Park, Hyesook Japan Epidemiological Association 2016 Journal of epidemiology Vol.26 No.3

<P><B>Background</B></P><P>Smoking is a major modifiable risk factor for premature mortality. Estimating the smoking-attributable burden is important for public health policy. Typically, prevalence- or smoking impact ratio (SIR)-based methods are used to derive estimates, but there is controversy over which method is more appropriate for country-specific estimates. We compared smoking-attributable fractions (SAFs) of deaths estimated by these two methods.</P><P><B>Methods</B></P><P>To estimate SAFs in 2012, we used several different prevalence-based approaches using no lag and 10- and 20-year lags. For the SIR-based method, we obtained lung cancer mortality rates from the Korean Cancer Prevention Study (KCPS) and from the United States-based Cancer Prevention Study-II (CPS-II). The relative risks for the diseases associated with smoking were also obtained from these cohort studies.</P><P><B>Results</B></P><P>For males, SAFs obtained using KCPS-derived SIRs were similar to those obtained using prevalence-based methods. For females, SAFs obtained using KCPS-derived SIRs were markedly greater than all prevalence-based SAFs. Differences in prevalence-based SAFs by time-lag period were minimal among males, but SAFs obtained using longer-lagged prevalence periods were significantly larger among females. SAFs obtained using CPS-II-based SIRs were lower than KCPS-based SAFs by >15 percentage points for most diseases, with the exceptions of lung cancer and chronic obstructive pulmonary disease.</P><P><B>Conclusions</B></P><P>SAFs obtained using prevalence- and SIR-based methods were similar for males. However, neither prevalence-based nor SIR-based methods resulted in precise SAFs among females. The characteristics of the study population should be carefully considered when choosing a method to estimate SAF.</P>

Monitoring of fish pathogens in maricultured salmonid fish

Kyoung-hui Kong,Myung-Joo Oh,Young-Ho Jeon,Ah-ra Kim,Wi-sik Kim 한국수산과학회 양식분과 2016 한국수산과학회 양식분과 학술대회 Vol.2016 No.10

Comprehensive Cancer Panel Sequencing Defines Genetic Diversity and Changes in the Mutational Characteristics of Pancreatic Cancer Patients Receiving Neoadjuvant Treatment

Kyong-Ah Yoon,Sang Myung Woo,Yun-Hee Kim,Sun-Young Kong,Min Kyoung Lee,Sung-Sik Han,Tae Hyun Kim,Woo Jin Lee,Sang-Jae Park 거트앤리버 소화기연관학회협의회 2019 Gut and Liver Vol.13 No.6

Background/Aims: Pancreatic ductal adenocarcinoma (PDA) is associated with an extremely poor prognosis. This study assessed the genetic diversity among patients with PDA and compared their mutational profiles before and after treatment. Methods: Tumors and matched blood samples were obtained from 22 PDA patients treated with neoadjuvant chemoradiation therapy. The somatic mutations were analyzed with comprehensive cancer gene panel (CCP). In addition, the biopsy samples obtained at diagnosis and the surgically resected samples after treatment were compared for seven patients. The CCP provided formalin-fixed paraffin-embedded sample-compatible multiplexed target selection for 409 genes implicated in cancer. Results: Assessments of the MLH1, MLH3, MSH2, and PMS2 genes showed that the four patients with the highest relative burdens of mutations harbored somatic mutations in at least three of these genes. Genes in the histone-lysine N-methyltransferase 2 (KMT2) family, such as KMT2D, KMT2A, and KMT2C, were frequently mutated in tumor samples. Survival was worse in patients with ARID1A gene mutations than those without ARID1A gene mutations. Mutation patterns were compared between tissue samples before and after neoadjuvant treatment in seven patients who underwent surgical resection. The allelic fraction of mutations in KRAS codon 12 was lower in the surgically resected samples than in the endoscopic ultrasonography-guided fine needle aspiration biopsy samples of six patients. The number of mutant alleles of the histone lysine methyltransferase gene WHSC1 also decreased after treatment. Conclusions: These results indicate that tumor tissue from PDA patients is genetically diverse and suggest that ARID1A mutations may be a potential prognostic marker for PDA.

학생부종합전형 지원자의 과학교과 선택과목과 지원학과 분석 : K대학교 네오르네상스전형 사례 중심

공자원 ( Kong Ja-won ),박윤경 ( Park Yun-kyoung ),박은아 ( Park Eun-ah ),이수영 ( Lee Soo-young ) 경희대학교 입학전형연구센터 2018 입학전형연구 Vol.7 No.-

7차 교육과정 이후 고등학교에서 과학교과는 선택적으로 운영·이수되고 있으나, 이수하는 과목 및 이수단위는 학교별 교육과정이나 각 개별 고등학교가 처해있는 상황에 따라 다르게 운영되고 있다. 학생부종합전형의 경우, 전형의 특성 상 학생이 진학하고자 하는 학과와 고등학교에서 선택해서 이수한 과목간의 연계가 중요하다. 이에, 본 연구에서는 K대학교 네오르네상스전형 지원자를 대상으로 과학교과 선택과목과 지원학과와의 연관성 및 서류평가 성적과의 관계 등을 분석하기 위해, 2017학년도 K대학교 학생부종합전형 지원자들이 고등학교 때 이수한 과학 선택과목과 서류평가 성적 및 합격 여부 등에 대한 데이터를 가지고 실질적인 결과를 분석하였다. 본 연구의 결과는 다음과 같다. 첫째, 학과의 특성에 따라 지원자와 합격자 간 과학Ⅱ 이수여부에 편차가 발생하는 경우가 있었으나, 이를 일반화하기는 어렵다. 둘째, 과학Ⅱ 이수여부에 따라 서류평가 성적 평균 차이 및 합격자 비율의 차이가 발생하는 모집단위가 있었다. 마지막으로 자연계열 전체를 대상으로 분석한 결과 물리Ⅱ와 지구과학Ⅱ의 경우 이를 이수한 경우가 이수하지 않은 경우보다 합격자 비율이 더 높은 것으로 나타났다. 본 연구의 의의는 고등학교에서 교육과정을 설계하거나 고등학생들이 과학과목 선택 시 참고자료로 활용하는 데 있다. Since the introduction of the selection-oriented curriculum, which was first introduced in the 7th curriculum, individual students have been given the choice of subjects until the revised curriculum in 2009. Science courses are selectively operated and completed in the education curriculum of high schools, but the courses and classes taken are managed differently depending on the curriculum or the situation in which individual high schools are located. In the case of comprehensive school records screening, the association between the departments the student wants to enter and the subjects he or she has chosen in high school is important due to the nature of the admission. Therefore, this study investigated and analyzed practical factors affecting high school students in choosing science subjects for K University's Neo-Renaissance candidates. The results of this study are as follows : First, although there have been deviations in the science II application between the applicant and the successful candidate depending on the characteristics of the department, it is difficult to generalize this. Second, there was a department where the difference in average evaluation of documents scores and the proportion of successful applicants varied depending on the number of science II applications. Finally, the analysis of the entire natural category showed that physical II and earth science II had a higher proportion of successful applicants than those who did not complete the course. The significance of this study is to design the curriculum in high schools or to use it as a reference for high school students to choose their science subjects.

Role of p-anisaldehyde in the Differentiation of C2C12 Myoblasts

Dal-Ah KIM,Kyoung Hye KONG,Hyun-Jeong CHO,Mi-Ran LEE 대한임상검사과학회 2023 대한임상검사과학회지(KJCLS) Vol.55 No.3

In this study, we investigated whether p-anisaldehyde (PAA), the main component of essential oils derived from anise seeds, influences the differentiation of mouse C2C12 myoblasts. Cells were induced to differentiate over 5 days using a differentiation medium with or without PAA (50 or 200 mg/mL). Myotube length and diameter were measured, and the expressions of myogenic markers (myoblast determination protein 1, myogenin, myocyte enhancer factor 2, muscle creatine kinase, and myosin heavy chain) and atrophy-related genes (atrogin-1 and muscle ring finger-1 [MuRF-1]) were assessed by quantitative real-time polymerase chain reaction. Additionally, protein kinase B (Akt) phosphorylation was monitored by western blotting. PAA significantly induced the formation of smaller and thinner myotubes and reduced myogenic marker expression. Furthermore, PAA increased the expressions of atrogin-1 and MuRF-1 and simultaneously reduced Akt phosphorylation. Our findings indicate that PAA inhibits the myogenic differentiation of C2C12 cells by reducing the phosphorylation and activation of Akt.

Outcome of Treatment of Advanced Anterior Vaginal Wall Prolapse with Transobturator Tension-Free Vaginal Mesh (Prolift(R): A Single Center Experience (초)

전경철 ( Kyoung Chul Chun ),( Sang Hyun Shin ),( Ju Youn Kong ),( Doo Young Chang ),( Young Ah Kim ),( Myung Kwon Jeon ),( Eung Soo Lee ) 대한산부인과학회 2010 대한산부인과학회 학술대회 Vol.96 No.-

Chromothripsis in Treatment Resistance in Multiple Myeloma

Lee, Kyoung Joo,Lee, Ki Hong,Yoon, Kyong-Ah,Sohn, Ji Yeon,Lee, Eunyoung,Lee, Hyewon,Eom, Hyeon-Seok,Kong, Sun-Young Korea Genome Organization 2017 Genomics & informatics Vol.15 No.3

Multiple myeloma (MM) is a malignant disease caused by an abnormal proliferation of plasma cells, of which the prognostic factors include chromosomal abnormality, ${\beta}$-2 microglobulin, and albumin. Recently, the term chromothripsis has emerged, which is the massive but highly localized chromosomal rearrangement in response to a one-step catastrophic event. Many studies have shown an association of chromothripsis with the prognosis in several cancers; however, few studies have investigated it in MM. Here, we studied the association between chromothripsis-like patterns and treatment resistance or prognosis. First, we analyzed nine MM cell lines (U266, MM.1S, RPMI8226, KMS-11, KMS-12-BM, KMS-12-PE, KMS-28-BM, KMS-28-PE, and NCI-H929) and bone marrow samples of four patients who were diagnosed with MM by next-generation sequencing-based copy number variation analysis. The frequency of the chromothripsis-like pattern was observed in seven cell lines. We analyzed the treatment-induced chromothripsis-like patterns in KMS-12-BM and KMS-12-PE cells. As a result, breakpoints and chromothripsis-like patterns were increased after drug treatment in the relatively resistant KMS-12-BM. We further analyzed the patients' results according to the therapeutic response, which was divided into sensitive and resistant, as suggested by the International Myeloma Working Group. The chromothripsis-like pattern was more frequently observed in the resistant group. In the sensitive group, the frequency of the chromothripsis-like pattern decreased after treatment, whereas the resistant group showed increased chromothripsis-like patterns after the treatment. These results suggest that the chromothripsis-like pattern is associated with treatment response in MM.