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Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
Ju-Won Kim,Bang Geun Oh,Julan Kim,Dong-Gyun Kim,Bo-Hye Nam,Young-Ok Kim,Jung Youn Park,JaeHun Cheong,Hee Jeong Kong 한국발생생물학회 2018 발생과 생식 Vol.22 No.3
A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.
Kong, Hee Jeong,Kim, Julan,Kim, Ju-Won,Kim, Hyun-Chul,Noh, Jae Koo,Kim, Young-Ok,Kim, Woo-Jin,Yeo, Sang-Yeob,Park, Jung Youn The Korean Society of Developmental Biology 2019 발생과 생식 Vol.23 No.1
To develop a promoter capable of driving transgene expression in non-model fish, we identified and characterized the muscle-specific alpha-actin gene in olive flounder, Paralichthys olivaceus (PoACTC1). The regulatory region of PoACTC1 includes putative regulatory elements such as a TATA box, two MyoD binding sites, three CArG boxes, and a CCAAT box. Microinjection experiments demonstrated that the regulatory region of PoACTC1, covering from -2,126 bp to +751 bp, just prior to the start codon, drove the expression of red fluorescent protein in developing zebrafish embryos and hatching olive flounder. These results suggest that the regulatory region of PoACTC1 may be useful in developing a promoter for biotechnological applications such as transgene expression in olive flounder.
Kong, Hee Jeong,Kim, Ju Lan,Moon, Ji Young,Kim, Woo-Jin,Kim, Hyung Soo,Park, Jung Youn,Cho, Hyun Kook,An, Cheul Min MDPI 2014 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.15 No.10
<P>The fish Vitellogenin (Vg) gene has been applied as a biomarker for exposure to estrogenic compounds in the aquatic environment. In this study, we cloned and characterized Vg cDNA from the Korean rose bitterling <I>Rhodeus uyekii</I> (Ru-Vg). The Ru-Vg cDNA encodes a 1424-amino-acid polypeptide that belongs to the VgAo1 family and contains a putative signal peptide, lipovitellin I, phosvitin, and lipovitellin II, but does not contain the vWFD domain or the <I>C</I>-terminal peptide. The deduced Ru-Vg protein has high amino acid identity (73.97%–32.17%) with fish Vg proteins. Pairwise alignment and phylogenetic analysis revealed that Ru-Vg is most closely related to <I>Acheilognathus yamatsutae</I> Vg. Ru-Vg transcripts were detected using quantitative polymerase chain reaction in all tissues tested, with the highest level of expression observed in the ovary. Ru-Vg mRNA was upregulated in <I>R. uyekii</I> hepatopancreas cells in response to treatment with 17β-estradiol (E2) or 17α-ethinylestradiol (EE2). Luciferase reporter expression, driven by the 5'-regulatory region of the Ru-Vg gene spanning from −1020 bp to the start codon was induced by the estrogen receptor and was synergistically activated by treatment with E2 or EE2. These results suggest that <I>R. uyekii</I> and the Ru-Vg gene may be useful as biomarkers for exposure to E2 or EE2.</P>
Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
Kim, Ju-Won,Oh, Bang Geun,Kim, Julan,Kim, Dong-Gyun,Nam, Bo-Hye,Kim, Young-Ok,Park, Jung Youn,Cheong, JaeHun,Kong, Hee Jeong The Korean Society of Developmental Biology 2018 발생과 생식 Vol.22 No.3
A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.
Kim, Ju-Won,Kong, Hee Jeong,Nam, Bo-Hye,Park, Jung Youn,Kim, Do-Hyung,Jeong, Ji-Min,Park, Chan-Il Elsevier 2017 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.76 No.-
<P><B>Abstract</B></P> <P>We isolated and characterised a cDNA encoding the lysosomal protective protein (serine protease) cathepsin A (CTSA) from rock bream (<I>Oplegnathus fasciatus</I>). The full-length rock bream CTSA (RbCTSA) cDNA (1814 bp) contains an open reading frame of 1419 bp that encodes 472 amino acids. Alignment of multiple CTSA protein sequences revealed that the active site serine and histidine residues were well-conserved among the other CTSA sequences. RbCTSA is highly expressed in the peripheral blood leukocytes, kidney, spleen, liver, intestine, gill, heart, brain, stomach, and eye. RbCTSA expression was also examined in several tissues, including whole kidneys and spleens, under bacterial and viral challenge. In general, all of the examined tissues that were infected with <I>Edwardsiella tarda</I>, <I>Streptococcus iniae</I>, or red sea bream iridovirus (RSIV) exhibited significant upregulation of RbCTSA expression compared to the controls. Our results reveal that RbCTSA may be involved in the immune responses of rock bream.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The cathepsin A (CTSA) cDNA was identified from a rock bream. </LI> <LI> The active site serine and histidine residues were well-conserved within the aligned sequence. </LI> <LI> RbCTSA gene was significantly expressed in the kidney, spleen, gill and intestine. </LI> <LI> RbCTSA upregulation was observed in response to pathogen infections. </LI> </UL> </P>
Lee, Hyewon,Kong, Sun-Young,Sohn, Ji Yeon,Shim, Hyoeun,Youn, Hye Sun,Lee, Sangeun,Kim, Hyun Ju,Eom, Hyeon-Seok Hindawi Publishing Corporation 2014 BioMed research international Vol.2014 No.-
<P>Red blood cell distribution width (RDW) is a parameter reported in complete blood cell count tests, and has been reported as an inflammatory biomarker. Multiple myeloma (MM) is known to be associated with inflammatory microenvironments. However, the importance of RDW has been seldom studied in MM. For this study, 146 symptomatic myeloma patients with available RDW at diagnosis were retrospectively reviewed, and their characteristics were compared between two groups, those with high (>14.5%) and normal (≤14.5%) RDW. RDW was correlated to hemoglobin, MM stage, <I><I>β</I></I>2-microglobulin, M-protein, bone marrow plasma cells, and cellularity (<I>P</I> < 0.001). During induction, overall response rates of the two groups were similar (<I>P</I> = 0.195); however, complete response rate was higher in the normal-RDW group than it was in the high-RDW group (<I>P</I> = 0.005). With a median follow-up of 47 months, the normal-RDW group showed better progression-free survival (PFS) (24.2 versus 17.0 months, <I>P</I> = 0.029) compared to the high-RDW group. Overall survival was not different according to the RDW level (<I>P</I> = 0.236). In multivariate analysis, elevated RDW at diagnosis was a poor prognostic factor for PFS (HR 3.21, 95% CI 1.24–8.32) after adjustment with other myeloma-related prognostic factors. RDW would be a simple and immediately available biomarker of symptomatic MM, reflecting the systemic inflammation.</P>
미디어에 제시된 과학관련 용어에 대한 학생 및 대중의 인식 수준 조사
최성연(Sung Youn Choi),문공주(Kong ju Mun),이현주(Hyun ju Lee) 학습자중심교과교육학회 2011 학습자중심교과교육연구 Vol.11 No.1
현대 사회는 빠르게 변화하고 있으며, 과학 기술과 깊이 연관되어 있다. 또한 다 양한 미디어를 통해 매일 과학기술 관련 용어를 접하고 이와 관련된 정보를 쉽게 얻을 수 있다. 이러한 사회 변화에 적응하는 21세기 세계시민의 자질을 갖추기 위 해서는 과학적 소양을 키워야 한다. 따라서 본 연구에서는 일반인들의 과학적 소양 을 탐색하기 위해 미디어에 나타난 과학 용어들에 대중의 이해도를 조사하였다. 연 구자들은 인터넷 검색엔진을 이용하여 뉴스, 신문기사, 영화, 문학작품에 가장 자주 나타나는 과학 용어들 중 반도체, 나노기술, 줄기세포, 환경호르몬, 빅뱅이론, 쓰나 미 등의 21개의 용어를 선정하고, 각 용어의 개념 이해를 측정할 수 있는 선다형 문 항을 개발하였다. 중학생 234명, 대학생 234 명, 학부모 233명이 미디어 속 과학 용 어 측정을 위한 설문에 참여하였으며, 수집된 설문 결과는 정답률과 평균점수를 바 탕으로 비교 분석하였다. 본 연구의 결과를 통해 대중이 과학 용어에 대하여 가지 는 관심과 인식 수준을 이해할 수 있었으며, 대중의 과학 이해를 높이기 위한 교육 적, 사회적, 문화적 노력에 대한 시사점을 얻을 수 있었다. Citizens in the contemporary society come in intimate contact with scientific terms through mass media, such as news coverage, movies, TV programs, and writings, which constitute a major source of new information. Although basic understanding terms of science and technology is often regarded as one of the major elements for scientific literacy, the public and students show lack of understanding on them. In this study, we identified twenty one scientific terms over the five areas (i.e. life science, environmental science, energy, materials, and earth science) that were frequently used in media, and measured the public and students understanding on the terms using multiple-choice test items. Participants included 234 undergraduate students, 234 middle school students, and 233 parents of the students. Results indicated that the average percentage of correct answers on the terms was 40%. The items that showed highest understanding were included in life science and environmental science areas. College students presented higher score than middle school students and their parents. Gender difference was not significant except college students understanding of earth science and energy areas.