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So Young Lee,Hwan Tae Lee,Woo-Jae Kwoun,Youn Sun Lee,안정열 대한임상검사정도관리협회 2020 Journal of Laboratory Medicine And Quality Assuran Vol.42 No.2
Background: The Sysmex DI-60 system (Sysmex, Japan) is an automated cell image analyzer. This study aimed to evaluate the performance of the DI-60 system for the differential analysis of leukocytes. Methods: A total of 220 samples were analyzed in this study. The agreement between DI-60 pre-classification and manual verification by experts was determined. The correlation between the differential leukocyte counts obtained using the DI-60 system and those manually obtained in the peripheral blood smears were determined. Results: The pre-classification agreement of DI-60 was 91.0%. The correlation coefficients of normal five-part differentials were 0.9163 (segmented neutrophils), 0.9017 (lymphocytes), 0.8533 (monocytes), 0.8345 (eosinophils), and 0.3505 (basophils). The sensitivity, specificity, positive predictive value, negative predictive value, and the efficiency of counting the abnormal cells, including blasts, promyelocytes, myelocytes, metamyelocytes, lymphocyte variants, and erythroblasts, were determined. The efficiency of the DI-60 system in counting the blasts, promyelocytes, myelocytes, metamyelocytes, lymphocyte variants, and erythroblasts was 99.5%, 100.0%, 95.9%, 96.5%, 98.6%, 100.0%, and 95.9%, respectively. Conclusions: The pre-classification agreement of DI-60 was higher than that of previous studies. The correlation between the differential leukocyte counts obtained with the DI-60 system and those of manual counting was acceptable. The performance of DI-60 as a screening tool in clinical laboratories may be good; however, it is yet to replace manual slide review.
정지훈,문송미,박필환,안정열,김경희,서자영,이환태,Kwoun Woo Jae,서일혜 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.5
Dear Editor, Nocardia species are uncommon pathogens that affect immunosuppressed patients; although cerebral nocardiosis is a rare condition, it is associated with significant morbidity and mortality [1]. Because Nocardia species exhibit different antibiotic susceptibilities, accurate species identification is important for prognoses. To the best of our knowledge, this is the first case of Nocardia asiatica brain abscesses reported in a systemic lupus erythematosus (SLE) patient.