PCR-RFLP 분석에 의한 무의 자가불화합성 유전자의 비교

최장선,김영호,강권규 안성산업대학교 2002 論文集 Vol.33 No.-

Self-incompatibility of Raphanus sativus is controlled by a single multi-allelic locus(S locus), which contains at least two highly polymophic genes expressed in the stigma: an S glycoprotein gene(SLG) and an S recetor kinase gene(SRK). The putative ligand-binding domain of SRK exhibits high homology to the secretory protein SLG, and it is believed that SLG and SRK form an active receptor kinase complex with a self-pollen ligand, which leads to the rejection of self-pollen. Among fifty-one inbred lines of R. sativus, fourteen S haplotypes were identified on the basis of DNA polymorphisms detected by PCR-RFLP analysis using SLG and SRK specific primer. PCR amplification was detected band in SLG 1.1~1.2Kb, SRK Ⅰ 0.8~0.9Kb, SRK Ⅱ 1.2~1.3Kb by analysis of the agaros gel electrophoresis. All different S genotypes showed different profiles in agarose electrophoresis, after digestion with restriction endonucleases. The nucleotide sequences of the fragments of these fourteen R. sativus SLG and SRK alleles were determined. Degree of similarity of the nucleotide sequences to a Brassica SLG and SRK ranged from 87% to 94%. Amino acid sequences deduced from these show about 50-55% homology to the putative amino acid sequences of SLG and SRK.

PCR 핵산 지문법에 의한 Dianthus속의 유연관계분석

홍준식,이문원,강권규 안성산업대학교 2002 論文集 Vol.33 No.-

This study was conducted to evaluate the ressibility of various Dianthus species as ornamental plants based on morphological characteristics and to analyze the genetic relationship among the Dianthus species. D. chinensis, D. curthusiamoum, D. arenorisus, D. superbus and D. barbatus possessing spendour flowering cluster with pink and red color could be suggested for garden plant, with D. caryophyllus having red flower clusters with linear leaf shape for pot flower or garden plant. Twelve oligonucleotide random primers were used to amplify genomic DNA of Dianthus species using polymerase chain reaction. Ninety-five polymorphic bands in the range of 300 to 3,500 bp were obtained from PCR fingerprinting. Similairity matrix of PCR fingerprinting profiles was generated by coefficient value, and the data were subjected to cluster analysis. Thirty-four Dianthus species analyzed were classified into 5 groups with similarity coefficient value of 0.84 and 12 groups with the value of 0.88. PCR fingerprinting rusults showed similar trends as the morphological characteristics of the plants.

Uniconazol처리로 誘起된 옥수수 遺傳子 ZmKT4과 ZmKT28의 cloning 및 sequencing

Kwon Kyoo Kang(姜權圭),Ill Sup Nau(盧一燮),Hyo Yeon Lee(李孝淵),Young Il Lee(李榮日),Seon Ha Lee(李善河),Kameya Toshiaki(龜谷壽昭) 한국육종학회 1995 한국육종학회지 Vol.27 No.1

The seedling obtained from uniconazol treatment showed dwarfism and tillering. In order to identify uniconazol-induced genes, cDNA hybridization probes were made from poly(A⁺) RNA isolated from uniconazol-treated seedlings and untreated inbred line seedling(IK), and the putatively regulated clones was detected by the differential screening of the cDNA libray. RNA hybridization experiment revealed that two of the clones were in fact induced by application of uniconazol. mRNAs of ca. 800 and 1,500 baseds was identified by isolated two clones (ZmKT4 and ZmKT28) and markedly increased from 48hr after uniconazol application. ZmKT4 clones showed the highest level of mRNA accumulation in tassels, ears and leaves, and the lowest level in roots. ZmKT28 clone is expressed in the stems, tassels, silks, ears and leaves but no transcripts was detected in roots. Two clone may be associated wit the cell elongation and phase of vegetative growth.

Gene Expression and Iron Accumulation in Progeny of Transformants Introduced Fpl Gene Encoding the Iron Storage Protein in Red Pepper (Capsicum annuum L.)

Kwon-Kyoo kang,Young-Ho Kim 한국자원식물학회 2001 Plant Resources Vol.4 No.1

To improve the iron content of red pepper, we have transferred the entire coding sequence of the ferritin gene(Fp1) into Capsicum annuum (L. cv. Chungyang and Bukang) by Agrobacterium mediated transformation. Transformants were found to contain the Fp1 gene at up to three loci, increased distinct iron content changes. In transgenic plants, iron content was as much as 7-fold to 8-folds greater than that of their untransformed counterparts. Furthermore, the R1 progenies from transformant(A7, A8) co-segregated into a 15:1 ratio for both Kanamycin resistance and genotype of high iron.

Raman signatures of spin-phonon coupling in a self-intercalated van der Waals magnet Mn3Si2Te6

Kwon Chang Il,Kim Kwangrae,Kim So Young,Susilo Resta A.,Kang Beomtak,Kim Kyoo,Kim Duck Young,Kim Jonghwan,Kim B.J.,Kim Jun Sung 한국물리학회 2023 Current Applied Physics Vol.53 No.-

We report the results of Raman spectroscopy on a self-intercalated van der Waals ferrimagnet Mn3Si2Te6. Using polarization-resolved Raman spectroscopy, we identify twelve phonon modes with Eg and A1g symmetries, in agreement with the first principle calculations. Below the ferrimagnetic transition temperature Tc ∼ 78 K, we observed a significant deviation of the phonon frequency from the anharmonic model, accompanied by a strong line broadening, indicating a substantial spin-phonon coupling in Mn3Si2Te6. Among twelve phonon modes, this spin-phonon coupling is found to be strong mostly in the out-of-plane vibrational modes. These results highlight the dominant role of the interlayer superexchange interaction in determining the magnetic properties, reflecting the self-intercalated van der Waals structure of Mn3Si2Te6.

Fermi surface reconstruction in CeTe2induced by charge density waves investigated via angle resolved photoemission

Kang, J.-S.,Kim, D. H.,Lee, H. J.,Hwang, Jihoon,Lee, Han-Koo,Kim, H.-D.,Min, B. H.,Lee, K. E.,Kwon, Y. S.,Kim, J. W.,Kim, Kyoo,Kim, B. H.,Min, B. I. American Physical Society 2012 Physical review. B, Condensed matter and materials Vol.85 No.8

백서에서의 지주막하 투여된 Clonidine 에 대한 Naloxone 의 길항작용에 관한 실험적 연구

권재영,백승완,김해규,정규섭,김인세,강순환 대한마취과학회 1996 Korean Journal of Anesthesiology Vol.31 No.5

Background: Clonidine depress the surge of sympathetic system outflow via central alpha 2 adrenergic effect. Still on a debate is the receptor relevant to analgesic effect of clonidine. Methods: Intrathecal catheter(PE-10, 10 cm in length) was inserted via the atlanto-occipital membrane and the tip of intrathecal catheter was allowed to reach at the lumbar area. At the fifth day after catheter insertion, all experimental animals were ramdomly divided to two groups. Clonidine (5 ㎍) in clonidine group and morphine (45 ㎍) in morphine group was administered into subarachnoid space 20 minutes before tail-clamping test. Heart rate and blood pressure changes were recorded during the experimental period. Then naloxone was given intravenously 5 minutes after the first tail-clamping test. In 2 minutes after that, the second tail clamping was done. Results: Results were as follows. First, comparing the highest blood pressure changes before and after administration of naloxone, the elevation of blood pressure was significant after administration of naloxone in morphine group(p$lt;0.05), but not in clonidine group. Second, comparing the change of heart rate, in morphine group there was significant elevation of heart rate befcre and after administration of naloxone. And comparing the highest elevation of heart rate, morphine group showed significant difference before and after naloxone administration(p$lt;0.05), but not in clonidine group. Conclusions: From above results, we assumed that the analgesic effect of clonidine was not related to the opiate receptor.

Analysis of Phylogenetic Relationships among Medicago Species by Proteins Banding Patterns and RFLP Markers

Kwon Kyoo Kang,Young Ho Kim,Il Sup Nou,Il Yong Shim 한국자원식물학회 1997 한국자원식물학회지 Vol.10 No.3

The relationship of nine Medicago species belonging to four subgenera were analyzed by using SDS-PAGE and restriction fragment length polymorphism (RELP) methodologies. Sixty-eight bands of alcohol and salt soluble proteins and 85-133 RFLP markers were used to estimate the genetic distance among the species. These species were clustered together at around 0.1 to 0.4 level of distance for both kind of markers, indicating that Medicago species have a large genetic similarity. A combined cluster diagram, at a dissimilarity level of 0.3, differentiated nine species in four groups: group 1, M. littoralis , M. truncatulam, M.scutellata and M. rigidula; group 2, M. sativa ; group 3, M. lupulina ; group 4, M. orbicularis, M. radiata and M. minima. All of them, but except for M. minima. corrensponded to the existing four subgenera of the genus Medicago classified by Lesins and Lesins(1979).The most similar species were M. littoralis and M. trucatula and the most dissimilar one was M. lupulina. In separate cluster diagrams based on RFLP and protein markers, some differences were observed. In the case of RFLP or DNA markers, M. sativa (alfalfa) was distantly clustered with other Medicago species. But in the case of protein markers, M. sativa was closely clustered with M. scutellata, M. littorulis and M. truncatula.

Metoclopramide 가 Thiopental 과 Propofol 의 최면용량에 미치는 영향

강화자,김건식,김동욱,최영규,신옥영,이두익,권무일,김동수,신광일 대한정맥마취학회 1999 정맥마취 Vol.3 No.3

서론: 선택적 D_2 dopamine 수용체의 길항제인 metoclopramide를 정주시 진정효과를 나타낸다. 따라서 본 연구에서는 metoclopramide 전처치가 전신 마취 유도제인 thiopental과 propofol의 최면 용량에 미치는 영향을 관찰하였다. 방법: 마취전투약을 하지 않은 성인환자 120명을 대상으로 thiopental군(60명)과 propofol군(60명)으로 나눈 후 각 군을 다시 20명씩 대조군, metoclopramide 0.1 ㎎/㎏ 전처치군과 metoclopramide 0.2 ㎎/㎏ 전처치군으로 나누어 마취유도 5분전에 실험약제를 투여한 후 thiopental과 propofol을 정주하면서 최면 종료점에서 최면 용량을 측정하였다. 결과: Thiopental군과 propofol군 모두에서 metoclopramide 0.2 ㎎/㎏을 전처치한 경우 최면용량의 유의한 감소를 보였다(p<0.05). 결론: Metoclopramide는 thiopental과 propofol의 최면 용량을 감소시켰으며, 이는 metoclopramide의 D_2 dopamine 수용체 길항 작용에 의한 것으로 사료된다. Background: Metoclopramide has been shown to decrease thiopental and propofol requirement for induction of anesthesia. Therefore we evaluated the effect of metoclopramide. Methods: The effect of metoclopramide (0.1 ㎎/㎏, 0.2 ㎎/㎏) on the hypnotic dose for thiopental and propofol were studied in unpremedicated 120 ASA physical statal Ⅰ or Ⅱ patients. Loss of the ability to open eye on verbal command was used as a hypnotic end-point. Results: Metoclopramide in dose of 0.2 ㎎/㎏ significanty decreased thiopental and propofol requirement for hypnosis (P<0.05). Conclusion: Metoclopramide reduced the hypnotic dose of thiopental and propofol in a dose dependent fashion.

Change of Gibberellin, IAA and Ethylene in Treated Seedlings with a Plant Growth Retardant (Uniconazol, S-327D) in Maize

Kang, Kwon-Kyoo,Kim, Young-Ho 안성산업대학교 1997 論文集 Vol.29 No.1

本 硏究는 植物生長抑制劑(S-327D)에 의해 處理된 옥수수 幼苗에서 지베레린, LAA 및 ethylene의 變化를 究明하고자 실시하였으며 結果는 다음과 같다. 1. S-327D을 옥수수 幼苗에 處理하면 줄기의 生長 및 生長이 減少함을 보였으며, 0.4ppm 處理함에 따라 生長이 50% 減少하였다. 2. 이런 生長의 減少는 지베레린 0.1ppm 處理함에 따라 回復하였으나, S-327D는 지베레린에 의해 誘導되는 줄기의 增加는 보이지 않았다. 3. S-327D 處理 옥수수 幼苗에서의 지베레린 이나 지베레린과 같은 基質의 量은 生長抑制 程度에 따라 減少하였다. 4. S-327D 處理된 옥수수 幼苗에서 LAA의 量은 control에 비해 減少하였으나, ethylene의 生産은 增加함을 보였다.