항암화학요법을 받는 암 환자에 대한 Nucare^R의 영양지지효과

조덕연,김현수,곽상혁,강정현,김철희,배광봉,김종숙,박상준,윤환중,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Purpose : The purpose of this study was to evaluate the effect of nutritional support with enteral liquid supplement in cancer patients receving chemotherapy for possible benefit in nutritional, immunologic and golbal function of patients. Patients and Methods : From October 1995 to February 1997, 30 advanced cancer patients receving chemotherapy were divided two roups. The Nycare group, in addition to normal diet, Nucare^R enteral supplement was given for 1week right after chemotherapy for the duration of 2 chemotherapy cycles. Control group received only normal diet without parenteral fluid supplement for 2 chemotherapy cycles. Results : Median ages were 53 end 56 years for Nucare group and Contrl group respectively. Performance scores was less than 2 by ECOG scale. All patients were stage Ⅲ or Ⅳ. The physical parameters, such as weight, arm muscle circumference(AMC) and triceps skin fold(TSF) were decreased in both groups after 2 cycles of anticancer therapy. but it was less severe in Nucare group(p<0.05). Serum transferrin was maintained in mild deficit state in Nucare group, whereas it aggravated form mild to moderate deficit in Control group(p<0.05). Serum albumin level increased in Nucare group without statistical significance. but it decreased from normal to mild deficit in Control group. Serum total protein did not change significantly in Nucare group. but in Control group, serum total protein was decreased from 7.24±0.9 to 6.52±0.5(P<0.05). Total lymphocyte count did not change significantly in both groups. Conclusion : This study shows that the nutritional support with Nucare^R was effective in the prevention of nutritional deficit status in patients receving a nticancer chemotherapy.

Immuno-Affinity Chromatography에 의한 B. thuringiensis H9B균주의 모기살충성 내독소 단백질의 정제

김광현,배수장,이광배 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

For purification of a 70kDa toxic protein of mosquitocidal delta-endotoxin from B. thuringiensis strain H9B, immuno-affinity chromatography was performed. After separation of 70kDa toxic proteins from the delta-endotoxin of the strain H9B on SDS-PAGE, the 70kDa toxic protein was subcutaneously injected into rabbit for making a polyclonal antibody. A anti-70kDa toxic protein was purified by a column chromatography packed with proteinA-sepharose 4B gels. The 70kDa toxic protein from delta-endotoxin of the strain H9B was also purified by an immuno-affinity chromatography packed with CNBr-activated sepharose 4B gels conjugated anti-70kDa toxic protein after elution with 1/10M citric acid-1/5M Na₂HPO₄buffer(pH3.2) containing 0.5M NaCl. The 70kDa toxic protein was purified through only one step-separation system, was demonstrated by SDS-PAGE and immunoblot.

모기유충에 살충력이 있는 Bacillus thuringiensis subsp. darmstadiensis 73E10-2의 delta-endotoxin의 화학적 처리에 따른 안정성

김광현,조경순,이광배 동의대학교 기초과학연구소 1992 基礎科學硏究論文集 Vol.2 No.1

The delta-endotoxin from B. thuringiensis subsp. darmstadiensis 73E10-2 was resistant to high concentration of salt (4M NaBr), organic solvents (50% acetone), denaturants (4M urea), and neutral detergents (10% triton X-100). In contrast, the toxin was inactivated by treating with charged detergents as well as guanidine hydrochloride or carbon tetra-chloride. The delta-endotoxin is not a sulfhydryl activated toxin, but modification of the lysine side chains eliminated toxicity against mosquito larvae.

수용성 청색색소를 생산하는 Azotobacter vinelandii A80 균주의 분리 및 특성

김병우,배수장,김광현,김영희 동의대학교 기초과학연구소 1996 基礎科學硏究論文集 Vol.6 No.1

For using additives of foods, or cosmetics, a strain A80 producing blue pigment was isolated from soil. The strain A80 was identified as a Azolobacter vinelandii based on morphological and physiological characteristics. The strain A80 was extracellulaly secreted the blue pigment on PYG agar plate, but not secreted it into PYG broth. And then, the strain A80 was extracellulaly secreted the blue pigment in PYG broth containing 2.0% chitin, while the strain A80 was not secreted the blue pigment in PYG broth containing 2.0% chitin and 1% NaCl simultaniously.

고속직렬 인터페이스를 이용한 분산제어 시스템 설계에 관한 연구

김승연,박광현 大田工業大學 1991 한밭대학교 논문집 Vol.8 No.2

분산된 다양한 공정이나 대규모 공정제어시스템에 데이타전송을 위하여 사용되는 종래의 병렬버스 방식에는 여러가지 제한적요소가 내재해 있다. 본 논문에서는, IBM 호한 PC-AT를 호스트컴퓨터로 하고, dDCM 320 아나로그 1/0보드, dDCM 335 디지탈 1/0보드, PCX344 마스터 인터페이스보드 등으로 구성되는 고속직렬 인터페이스를 이용하여 계층적인 간단한 분산제어 시스템을 구현하였다. 또한, 입증된 데이타 링크프로토콜인 IBM 표준프로토콜 SDLC를 이용하여, SDLC 프로토콜의 중요기능인 Zero Bit Insertion/deletion 기능을 확인검증하였다. 이 논문에 제안된 고속직렬 인터페이스를 이용한 분산제어 시스템은 병렬버스방식의 단일제 어시스템보다 효율적임이 실증되었다. There are several limitations inherent in traditional parallel-bus mode used for the data transmission in the large scaled and various distributed process control system. In this paper, simple hierachical distributed control system is implemented with using a high-speed parallel interface consists of the IBM compatible PC -AT as host computer, dDCM 320 analog I/O board, dDCM 335 digital I/O board, and so forth. Moreover, the Zero bit inserion/deletion function which is important function of the SDLC protocol is verified using the proven protocol, SLDC of IBM. The distributed Control system with high-speed serial interface which is presented in this paper is testified in efficiency, comparing to a single control system with parallel-bub mode.

모기유충에 대한 살충성 내독소와 항진균성 물질을 동시에 생산하는 B. thuringiensis AF6균주의 분리 및 특성

김광현,이광배,신두만 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

For a biological control on a plant pathogen, Pyricularia oryzae, and a mosquito, Aedes aegypti, Bacillus thuringiensis strain AF6 which produces parasporal inclusion, delta-endotoxin, was isolated. The B. thuringiensis strain AF6 was produced not only an antifungal substance(AFS) against P. oryzae, but also a mosquitocidal delta-endotoxin. The AFS of the strain AF6 is more stable at pH4.0 than pH10.0. At the mode of action, the AFS of the strain AF6 was inhibited hypha growth on potato agar plate(pH5.0), and degraded cell walls of P. oryzae.

모기 살충성 세균 B. thuringiensis subsp. israelensis의 효과적인 분리 방법

김광현,이광배,신두만 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

For more convenient and rapidly isolation of Bacillus thuringiensis subsp. israelensis(Bti), 1) heat treatment for spore forming bacteria, 2) growth in enrichment culture media for Bacillus sp. and 3) selection of bacteria producing a lecithinase for Bacillus thuringiensis subsp. israelensis, were performed. Spore forming bacteria were counted 4.8×10^8cells/g soil on NAPGCY media, 9.2×10^7cells/g soil on NA media, and 3.6×10^8cells/g soil on NAAC media, respectively.; Bacteria producing only a lecithinase were reached at 25.2% on medium contained egg york, bacteria only producing a delta-endotoxin were reached at 23.2% by phase contrast microscope, and bacteria producing a lecithinase & a delta-endotoxin simulten-iously were reached at 13.7%. Bacillus thuringiensis which producing a lecithinase and a delta-endotoxin simultaniously among bacteria producing a lecithinase, were reached at 56.5%; A half of Bacillus thuringiensis was produced a delta-endotoxin, but not produced a lecithinase. Among 8 isolates of Bacillus thuringiensis, two strain of Bti which has a mosquito-cidal toxin, were detected by PCR using a specific primer of δ-endotoxin gene form Bti.

남자 만성 정신분열증 환자에서 Risperidone 투여에 의한 치료반응과 혈장HVA 및 5-HIAA 농도와의 관계

김형섭,조숙행,강대엽,박성덕,김광현 용인정신의학연구소 1997 용인정신의학보 Vol.4 No.2

수용성 황색색소를 분비하는 Bacillus sp. PY123균주의 분리 및 특성

김광현,김지연 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

To develop a yellow pigment for a food-additive, a strain producing a water-soluble yellow pigment was isolated from phylloplane of tree leaf. The strain PY123 was identified a Bacillus sp. based on morphological and biochemical characterization such as a bacillus form, mortility, spore formation, Gram positive, and catalase production. The pigment production of the strain PY123 was increased about 3 times in potato broth containing 1% sucrose and 0.1 mM CoCl₂than in only potato broth after incubation at 30℃ for 2 days. When 0.1 mM CoCl₂was added in potato broth containing 1% sucrose at late log phase during incubation of the strain PY123, cell growth was not inhibited, and the period at maximal pigment production of the strain PY123 was about 12 hrs faster than in potato broth containing 1% sucrose and 0.1 mM CoCl₂simultaniously.

한식 잔반처리를 위한 호기성 미생물의 분리 및 그 분해효과

김광현,김지연,이광배 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

For the treatment of Korea food-wastes, three mesophilic and one thermophilic bacteria were isolated from soil and fermented fertilizers. The thermophilic Streptomyces sp. strain WF021 produced two enzymes which were a protease and a lipase at 55℃. The mesophilic Bacillus sp. strain WF024 produced four enzymes which were a protease, a lipase, a amylase and a cellulase when the strain was grown both at 30℃ and 55℃. The Bacillus sp. PY123 had produced three enzymes which were a protease, a cellulase and a lipase at 30℃. The Bacillus sp. strain CM1 produced three enzymes which were a protease, a amylase, and a cellulase at 30℃. The bacteria were grown in media containing 6% NaCl at least and did not have antagonism each other. The four isolates treated much more food-wastes than referance strains did. In a flask without aeration, three reference strains treated 15.4% of food-wastes, while four isolated treated 23.7% of food-wastes. In a flask with aeration, food-wastes were treated 67.3% by four isolates, and 64.3% by three reference strains, but 53.9% without bacteria. However, food-wastes were treated about 78% in a 200ℓ-reactor made by Siwon Co., while 65.8% in a 20ℓ-reactor made by Sanyo Co.