개에 있어서 耳根部의 Lidocaine 水針에 의한 全身 無痛覺의 誘發

권현주,유건주,이상은,이정연,송근호,김명철,정성목,박성준,김덕환 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Twelve clinically healthy mongrel dogs were used to clarify the possibility of general analgesia induction by lidocaine aquapuncture to auricular root area in the present study. The experimental animals were devided into control (6 heads) and experimental groups (6 heads), respectively. Physiological salines (3 ml/kg) were injected into both auricular root areas in control group. Lidocaine hydrochlorides (3 ml/kg) were injected into both auricular root areas in experimental group. Superficial pain, the changes of vital signs and blood leukocytes, and PCV were examined on pre, 5 min., 15 min., 30 min, and 60 min. after injection in control and experimental groups, respectively. Analgesia was not detected at all in control group, however, all experimental animals were recumbent and analgesia was generally detected in the auricles, noses, dorsal parts, ventral areas, limbs and tails from 5 to 30 minutes after injection with lidocaine hydrochloride into auricular root areas in experimental group. As for the change of vital signs, body temperature showed decreasing tendency from 5 minutes after lidocaine injection in experimental group. Heart rates and respiration rates were increased on 5 minutes after lidocaine injection and then they showed decreasing tendency in experimental group. No significant difference were found among groups. As for the changes of hematological findings, the numbers of blood leukocytes were increased till 15 minutes after lidocaine injection and then they showed decreasing tendency. PCV values were increased on 5 minutes after lidocaine injection, and then they showed decreasing tendency. However, no significant differences were observed among groups. Considering the above findings, it was thought that general analgesia was possible by aquapuncture with lidocaine hydrochloride into auricular root areas and lidocaine aquapuncture didn’t affect so much to the changes of vital signs and blood.

The High Expressed Serum Soluble Neural Cell Adhesion Molecule, a High Risk Factor Indicating Hepatic Encephalopathy in Hepatocelular Carcinoma Patients

Liu, Tian-Hua,Guo, Kun,Liu, Ri-Qiang,Zhang, Shu,Huang, Zhuo-Hui,Liu, Yin-Kun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Objective: To investigate whether the expression of serum soluble neural cell adhesion molecule (sNCAM) is associated with hepatic encephalopathy (HE) in hepatocelular carcinoma (HCC) patients. Materials and Methods: The Oncomine Cancer Microarray database was used to determine the clinical relevance of NCAM expression in different kinds of human cancers. Sera from 75 HCC cases enrolled in this study were assessed for expression of sNCAM by enzyme linked immunosorbent assay (ELISA). Results: Dependent on the Oncomine Cancer Microarray database analysis, NCAM was down regulated in 10 different kinds of cancer, like bladder cancer, brain and central nervous system cancer, while up-regulated in lung cancer, uterine corpus leiomyoma and sarcoma, compared to normal groups. Puzzlingly, NCAM expression demonstrated no significant difference between normal and HCC groups. However, we found by quantitative ELISA that the level of sNCAM in sera from HCC patients with HE ($347.4{\pm}151.9ng/ml$) was significantly more up-regulated than that in HCC patients without HE ($260.3{\pm}104.2ng/ml$), the p-value being 0.008. sNCAM may be an important risk factor of HE in HCC patients, the correlation coefficients was 0.278 (P<0.05) on rank correlation analysis. Conclusions: This study highlights that up-regulated level of serum sNCAM is associated with HE in HCC patients and suggests that the high expression can be used as an indicator.

Active control of a nonlinear and hysteretic building structure with time delay

Kun Liu,Long-Xiang Chen,Guo-Ping Cai 국제구조공학회 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.40 No.3

Time delay inevitably exists in active control systems, and it may cause the degradation of control efficiency or instability of the systems. So time delay needs to be compensated in control design in order to eliminate its negative effect on control efficiency. Today time delay in linear systems has been more studied and some treating methods had been worked out. However, there are few treating methods for time delay in nonlinear systems. In this paper, an active controller for a nonlinear and hysteretic building structure with time delay is studied. The nonlinear and hysteretic behavior of the system is illustrated by the Bouc-Wen model. By specific transformation and augmentation of state parameters, the motion equation of the system with explicit time delay is transformed into the standard state space representation without any explicit time delay. Then the fourth-order Runge-Kutta method and instantaneous optimal control method are applied to the controller design with time delay. Finally, numerical simulations and comparisons of an eight-story building using the proposed time-delay controller are carried out. Simulation results indicate that the control performance will deteriorate if time delay is not taken into account in the control design. The simulations also prove the proposed time delay controller in this paper can not only effectively compensate time delay to get better control effectiveness, but also work well with both small and large time delay problems.

Numerical simulation in time domain to study cross-flow VIV of catenary riser subject to vessel motion-induced oscillatory current

Liu, Kun,Wang, Kunpeng,Wang, Yihui,Li, Yulong The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.1

The present study proposes a time domain model for the Vortex-induced Vibration (VIV) simulation of a catenary riser under the combination of the current and oscillatory flow induced by vessel motion. In this model, the hydrodynamic force of VIV comprises excitation force, hydrodynamic damping and added mass, which are taken as functions of the non-dimensional frequency and amplitude ratio. The non-dimensional frequency is related with the response frequency, natural frequency, lock-in range and the fluid velocity. The relatively oscillatory flow induced by vessel motion is taken into account in the fluid velocity. Considering that the added mass coefficient and the non-dimensional frequency can affect each other, an iterative analysis is conducted at each time step to update the added mass coefficient and the natural frequency. This model is in detail validated against the published test models. The results show that the model can reasonably reflect the effect of the added mass coefficient on the VIV, and can well predict the riser's VIV under stationary and oscillatory flow induced by vessel motion. Based on the model, this study carries out the VIV simulation of a catenary riser with harmonic vessel motion. By analyzing the bending moment near the touchdown point, it is found that under the combination of the ocean current and oscillatory flow the vessel motion may decrease the VIV response, while increase the excited frequencies. In addition, the decreasing rate of the VIV under vessel surge is larger than that under vessel heave at small vessel motion velocity, while the situation becomes opposite at large vessel motion velocity.

Phenolic composition and neuroprotective effects of the ethyl-acetate fraction from Inonotus sanghuang against H2O2-induced apoptotic cell death of primary cortical neuronal cells

Kun Liu,Cuilian Qi,Yihang Liu,Huagang Hu,Xuan Xiao,Junpeng Wang,Yaping Huai 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.9

The study aimed to characterize phenolic compounds of the Inonotus sanghuang’s ethyl-acetate fraction (EAF) and assess the neuroprotective effect of EAF using the H2O2-treated primary cortical neuronal cells (PCNC) model. Using HPLC-ECD, 5 phenolics were identified and quantified from EAF. H2O2-treated PCNC experiments in vitro showed that pretreatment with EAF increased the GSH-PX and SOD activities and reduced the NO, MDA, and Aβ contents. Furthermore, EAF suppressed the production of IL-1β, IFN-γ, IL-6, and TNF-α in H2O2-treated PCNC. Other mechanisms found that EAF reduced Bax, caspase 9, and caspase 3 expressions at the mRNA and protein levels while increasing Bcl-2 expression at the mRNA and protein levels. These results showed that EAF could serve as potential agents for anti-NDD.

Numerical simulation in time domain to study cross-flow VIV of catenary riser subject to vessel motion-induced oscillatory current

Liu, Kun,Wang, Kunpeng,Wang, Yihui,Li, Yulong The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.-

The present study proposes a time domain model for the Vortex-induced Vibration (VIV) simulation of a catenary riser under the combination of the current and oscillatory flow induced by vessel motion. In this model, the hydrodynamic force of VIV comprises excitation force, hydrodynamic damping and added mass, which are taken as functions of the non-dimensional frequency and amplitude ratio. The non-dimensional frequency is related with the response frequency, natural frequency, lock-in range and the fluid velocity. The relatively oscillatory flow induced by vessel motion is taken into account in the fluid velocity. Considering that the added mass coefficient and the non-dimensional frequency can affect each other, an iterative analysis is conducted at each time step to update the added mass coefficient and the natural frequency. This model is in detail validated against the published test models. The results show that the model can reasonably reflect the effect of the added mass coefficient on the VIV, and can well predict the riser's VIV under stationary and oscillatory flow induced by vessel motion. Based on the model, this study carries out the VIV simulation of a catenary riser with harmonic vessel motion. By analyzing the bending moment near the touchdown point, it is found that under the combination of the ocean current and oscillatory flow the vessel motion may decrease the VIV response, while increase the excited frequencies. In addition, the decreasing rate of the VIV under vessel surge is larger than that under vessel heave at small vessel motion velocity, while the situation becomes opposite at large vessel motion velocity.

Influence of Cooling Rate on Secondary Phase Precipitation and Proeutectoid Phase Transformation of Micro-Alloyed Steel Containing Vanadium

Kun Dou,Lingtao Meng,Qing Liu,Bo Liu,Yunhua Huang 대한금속·재료학회 2016 METALS AND MATERIALS International Vol.22 No.3

During continuous casting process of low carbon micro-alloyed steel containing vanadium, the evolution of strand surface microstructure and the precipitation of secondary phase particles (mainly V(C, N)) are significantly influenced by cooling rate. In this paper, influence of cooling rate on the precipitation behavior of proeutectoid α-ferrite at the γ-austenite grain boundary and in the steel matrix are in situ observed and analyzed through high temperature confocal laser scanning microscopy. The relationship between cooling rate and precipitation of V(C, N) from steel continuous casting bloom surface microstructure is further studied by scanning electron microscopy and electron dispersive spectrometer. Relative results have shown the effect of V(C, N) precipitation on α-ferrite phase transformation is mainly revealed in two aspects: (i) Precipitated V(C, N) particles act as inoculant particles to promote proeutectoid ferrite nucleation. (ii) Local carbon concentration along the γ-austenite grain boundaries is decreased with the precipitation of V(C, N), which in turn promotes α-ferrite precipitation.

Power Flow Control at the Subnetwork-Level in Microgrids

Kun Liu,Muhammad Mansoor Khan,Ahmad Rana,Dong Fei 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.2

This paper presents the idea of a smart load that can adjust the input power flow based on the intermittent power available from RESs (Renewable Energy Resources) to regulate the line voltage, and draw a constant power from the grid. To this effect, an innovative power flow controller is presented based on a Resistive ES (Electric Spring) in combination with a PEAT (Power Electronics based Adjustable Transformer), which can effectively shape the load power flow at the subnetwork level. With a PEAT incorporated in the step down transformer at the grid side, the proposed controller can supply non-critical loads through local RESs, and the critical loads can draw a relatively constant power from the grid. If there is an abundance of power produced by the RESs, the controller can supply both non-critical loads and critical loads through the RES, which significantly reduces the power demand from the grid. The principle, practicality, stability analysis, and controller design are presented. In addition, simulation results show that the power flow controller performs well in shaping the load power flow at the subnetwork level, which decreases the power demand on the grid. Experimental results are also provided to show that the controller can be realized.

Discovery of nitrate–CPK–NLP signalling in central nutrient–growth networks

Liu, Kun-hsiang,Niu, Yajie,Konishi, Mineko,Wu, Yue,Du, Hao,Sun Chung, Hoo,Li, Lei,Boudsocq, Marie,McCormack, Matthew,Maekawa, Shugo,Ishida, Tetsuya,Zhang, Chao,Shokat, Kevan,Yanagisawa, Shuichi,Sheen, Nature Publishing Group, a division of Macmillan P 2017 Nature Vol.545 No.7654

Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca<SUP>2+</SUP> signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis leaves and roots. A nitrate-sensitized and targeted functional genomic screen identifies subgroup III Ca<SUP>2+</SUP>-sensor protein kinases (CPKs) as master regulators that orchestrate primary nitrate responses. A chemical switch with the engineered mutant CPK10(M141G) circumvents embryo lethality and enables conditional analyses of cpk10 cpk30 cpk32 triple mutants to define comprehensive nitrate-associated regulatory and developmental programs. Nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation. Conditional cpk10 cpk30 cpk32 and nlp7 mutants similarly impair nitrate-stimulated system-wide shoot growth and root establishment. The nutrient-coupled Ca<SUP>2+</SUP> signalling network integrates transcriptome and cellular metabolism with shoot–root coordination and developmental plasticity in shaping organ biomass and architecture.

Power Flow Control at the Subnetwork-Level in Microgrids

Liu, Kun,Khan, Muhammad Mansoor,Rana, Ahmad,Fei, Dong The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.2

This paper presents the idea of a smart load that can adjust the input power flow based on the intermittent power available from RESs (Renewable Energy Resources) to regulate the line voltage, and draw a constant power from the grid. To this effect, an innovative power flow controller is presented based on a Resistive ES (Electric Spring) in combination with a PEAT (Power Electronics based Adjustable Transformer), which can effectively shape the load power flow at the subnetwork level. With a PEAT incorporated in the step down transformer at the grid side, the proposed controller can supply non-critical loads through local RESs, and the critical loads can draw a relatively constant power from the grid. If there is an abundance of power produced by the RESs, the controller can supply both non-critical loads and critical loads through the RES, which significantly reduces the power demand from the grid. The principle, practicality, stability analysis, and controller design are presented. In addition, simulation results show that the power flow controller performs well in shaping the load power flow at the subnetwork level, which decreases the power demand on the grid. Experimental results are also provided to show that the controller can be realized.