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( Agata Malinowska ),( Sylwia Kowalczyk1 ),( Zdzis Aw Targo Ski ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.3
The identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein. Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.
THE SHARP BOUND OF THE THIRD HANKEL DETERMINANT FOR SOME CLASSES OF ANALYTIC FUNCTIONS
Kowalczyk, Bogumila,Lecko, Adam,Lecko, Millenia,Sim, Young Jae Korean Mathematical Society 2018 대한수학회보 Vol.55 No.6
In the present paper, we have proved the sharp inequality ${\mid}H_{3,1}(f){\mid}{\leq}4$ and ${\mid}H_{3,1}(f){\mid}{\leq}1$ for analytic functions f with $a_n:=f^{(n)}(0)/n!$, $n{\in}{\mathbb{N}},$, such that $$Re\frac{f(z)}{z}>{\alpha},\;z{\in}{\mathbb{D}}:=\{z{\in}{\mathbb{C}}:{\mid}z{\mid}<1\}$$ for ${\alpha}=0$ and ${\alpha}=1/2$, respectively, where $$H_{3,1}(f):=\left|{\array{{\alpha}_1&{\alpha}_2&{\alpha}_3\\{\alpha}_2&{\alpha}_3&{\alpha}_4\\{\alpha}_3&{\alpha}_4&{\alpha}_5}}\right|$$ is the third Hankel determinant.
The sharp bound of the third Hankel determinant for some classes of analytic functions
Bogumi la Kowalczyk,Adam Lecko,Millenia Lecko,심영재 대한수학회 2018 대한수학회보 Vol.55 No.6
In the present paper, we have proved the sharp inequality $|H_{3,1}(f)|$ $\le 4$ and $|H_{3,1}(f)|\le 1$ for analytic functions $f$ with $a_n:=f^{(n)}(0)/n!,\ n\in\mathbb{N},$ such that $$\mathrm{Re}\, \frac{f(z)}{z}> \alpha,\quad z\in\mathbb{D}:=\{z \in\mathbb{C} : |z|<1\}$$ for $\alpha=0$ and $\alpha=1/2,$ respectively, where \begin{equation*} H_{3,1}(f):= \begin{vmatrix} a_1 & a_2 & a_3 \\ a_2 & a_3 & a_4 \\ a_3 & a_4 & a_5 \end{vmatrix} \end{equation*} is the third Hankel determinant.
Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity
Ewelina Łojewska,Tomasz Sakowicz,Aleksandra Kowalczyk,Magdalena Konieczka,Janina Grzegorczyk,Przemysław Sitarek,Ewa Skała,Piotr Czarny,Tomasz Śliwiński,Tomasz Kowalczyk 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1
Antibiotic-resistant microorganisms causing a life-threatening infection pose a serious challenge for modern science. The rapidly growing number of incidents for which the use of standard antibiotics is ineffective forces us to develop new alternative methods of killing microorganisms. Antimicrobial proteins and peptides (AMPs) can be promising candidates to solve this problem. Colicin-M is one of the representatives of this group and is naturally produced by Escherichia coli acting on other closely related bacterial strains by disrupting their outer cell membrane. This bacteriocin has huge potential as a potent antimicrobial agent, especially, since it was recognized by the FDA as safe. In this work, we present the expression of colicin M in model transgenic Nicotiana tabacum L. plants. We demonstrate that purified colicin retains its antibacterial activity against the control Escherichia coli strains and clinical isolates of Escherichia coli and Klebsiella pneumoniae. Our results also show that plant-derived ColM is not toxic for L929 and HeLa cell line, which allows us to suppose that plant-based expression could be an alternative production method of such important proteins.
ADHESION , REACTION AND STABILITY OF METAL/POLYIMIDE
Kim, J.,Kowalczyk, S.,Kim, Y. H.,Oh, T. S.,Molis, S. 대한전자공학회 1989 ICVC : International Conference on VLSI and CAD Vol.1 No.1
Interfacial reactions of polyimide with several metals have been investigated by XPS and TEM to determine their effects on adhesion and long term stability. It has been found that PI-on-metal interface and metal-on-PI interface are basically different arid have their own reaction mechanism. Both interfaces are susceptible to T/H environment resulting in significant adhesion loss and change of properties of polyimide. A great care is, therefore, necessary to insure a stable interface and reliability of devices.
Self-assembly of like-charged nanoparticles into microscopic crystals
Pillai, P.,Kowalczyk, B.,Grzybowski, B. Royal Society of Chemistry 2016 Nanoscale Vol.8 No.1
<P>Like-charged nanoparticles, NPs, can assemble in water into large, faceted crystals, each made of several million particles. These NPs are functionalized with mixed monolayers comprising ligands terminating in carboxylic acid group ligands as well as positively charged quaternary ammonium ligands. The latter groups give rise to electrostatic interparticle repulsions which partly offset the hydrogen bonding between the carboxylic acids. It is the balance between these two interactions that ultimately enables self-assembly. Depending on the pH, the particles can crystallize, form aggregates, remain unaggregated or even - in mixtures of two particle types - can 'choose' whether to crystallize with like-charged or oppositely charged particles.</P>
Małgorzata Kikowska,Mariusz Kowalczyk,Anna Stochmal,Barbara Thiem 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.1
We investigated the conditions leading to the accumulation of saponins in plants using in vitro cultures of Eryngium planumL. The aim was to establish a system that produced saponins of the same quality as those found in fi eld-grown plants and toenhance their accumulation in the laboratory. We observed that the content of saponins in organs from in vitro plantlets andplants grown in an experimental plot after micropropagation was higher (up to 8.7 times) compared with plants from naturalsites. Untreated callus accumulated saponins in amounts similar to those of the roots from fi eld-grown plants. Applyingsucrose to the growth medium at higher concentrations (40, 50, or 60 g L −1 ) resulted in enhanced saponin production in callus(1.2–1.7-fold). The elicitation with 100 μmol L −1 methyl jasmonate of callus grown on Murashige and Skoog medium supplementedwith 30 g L −1 sucrose resulted in a 1.2-fold increased saponin accumulation compared with callus maintained onthe same medium (30 g L −1 sucrose). This study provides the fi rst evidence that triterpenoid saponins can be biosynthesizedin in vitro plantlets and dediff erentiated cultures of E. planum .