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      • SCIESCOPUSKCI등재

        A Study on Effect of Carrying FecB Gene on Body Weight in Garole and Garole×Malpura Sheep

        Kolte, A.P.,Mishra, A.K.,Kumar, S.,Arora, A.L.,Singh, V.K. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.10

        High prolificacy in Garole sheep is due to existence of FecB mutation in an autosomal gene, bone morphogenetic protein receptor. The mutation enhances ovulation rate and in turn litter size in Garole sheep. Garole sires were crossed with non-prolific Malpura ewes with the aim to introduce prolificacy into Garole${\times}$Malpura (G${\times}$M) crosses through FecB introgression programme. In the present study, the effect of carrying booroola allele on litter size and live body weight was analyzed. The average litter size at birth was found to be 1.87 and 1.48 in the Garole and the G${\times}$M crosses, respectively. At weaning, 6-month, 9-month and 12-month of age, body weights were not affected by the presence of booroola allele (p>0.05); however, a significant effect (p<0.05) was found on body weight at birth in G${\times}$M crosses. In Garole sheep, no significant effect of FecB was observed on live weights in any age group. The interaction between the genetic group and the FecB genotype was also found to be non-significant.

      • KCI등재

        Thickness of the Ligamentum Flavum: Correlation with Age and Its Asymmetry–An Magnetic Resonance Imaging Study

        Vrushali Subhash Kolte,Seema Khambatta,Medha Vijay Ambiye 대한척추외과학회 2015 Asian Spine Journal Vol.9 No.2

        Study Design: A retrospective radiological study of the ligamentum flavum (LF). Purpose: This study is an attempt to measure and compare the thickening of the LF on both the sides with the use of magnetic resonance imaging, to investigate if there is a predominant tendency to thicken a specific side and also to determine if a correlation between the thickening of the LF and increasing age exists. Overview of Literature: Even though many studies measured the thickness of the LF, very few have compared it on each side, or determined its correlation with age. Methods: The thickness of LF was measured at the L3–4, L4–5, L5–S1 levels on both sides using the magnetic resonance images of 200 patients (n=1,200). The sample population was divided into three groups: 21–40 years, 41–60 years, and 61–80 years. The data was analyzed statistically, comparing the thickness of LF on both sides and in various age-groups. Results: The thickness of the LF was found to increase with age; however, there were several younger instances with thicknesses >4 mm. The mean thickness of the right LF at different spinal levels was measured (L3–L4=3.38±0.94 mm, L4–L5=3.70±1.16 mm, and L5–S1=3.65±1.16 mm) while the mean thickness of the left LF was higher (L3–L4=3.52±0.99 mm, L4–L5=3.84±1.12 mm, and L5– S1=3.78±1.24 mm). Conclusions: The LF thickness does not appear to have any side dominance; however, it tends to thicken with increasing age.

      • KCI등재

        Low frequency magnetoelectric effect in Bi0.5Na0.5TiO3-Ni0.5Zn0.5Fe2O4 particulate composites

        Parminder Singh,Radhapiyari Laishram,Jayant Kolte,Jayant Kolte,Puneet Sharma 대한금속·재료학회 2023 ELECTRONIC MATERIALS LETTERS Vol.19 No.5

        We report structural, dielectric, ferroelectric, magnetic, and low frequency magnetoelectric ( ME ) properties of (1− x ) Bi 0.5 Na 0.5 TiO 3 (BNT)– x Ni 0.5 Zn 0.5 Fe 2 O 4 (NZFO) (x = 0.05–0.30) microwave sintered particulate composites. Distinct phases of BNT and NZFO were confi rmed by X-ray diff raction and scanning electron microscopy. Raman spectroscopy measurement showed the absence of micro-strains within the composite. The temperature dependent dielectric studies revealed the ferroelectric to anti-ferroelectric transition at 220 °C and anti-ferroelectric to paraelectric transition at 320 °C. The ac conductivity showed both frequency dependent and independent behavior. Temperature dependent dc conductivity showed that upto 200 °C charge conduction is due to hopping of electrons, whereas at higher temperature diff usion of oxygen vacancies are responsible for the conduction. Ferroelectric and leakage current density measurements showed enhanced conduction losses with NZFO content. The maximum ME coeffi cient at 10 Hz frequency is obtained for 0.80BNT–0.20NZFO (4.33 mV/ cm.Oe at 800 Oe).

      • SCIESCOPUSKCI등재

        Diagnosis of Benzimidazole Resistance in Haemonchus contortus of Sheep by Allele Specific PCR

        Tiwari, J.,Kolte, A.P.,Kumar, S.,Swarnkar, C.P.,Singh, D.,Pathak, K.M.L. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.1

        The study was conducted on 162 adult male Haemonchus contortus of sheep collected from Avikanagar, Jaipur and Bikaner regions to diagnose the benzimidazole (BZ) resistance in H. contortus. The BZ resistance is primarily linked with the mutation in ${\beta}$-tubulin isotype 1 gene which substitute phenylalanine (Phe) into tyrosine (Tyr) at the 200 codon of the gene. An allele specific polymerase chain reaction (AS-PCR) technique was used for diagnosis of BZ resistance in H. contortus. In AS-PCR, one reverse primer (TGG 312) was used in two separate reactions with each of 2 forward primers (resistant TGG 331 and susceptible CAW 106 primer) that differed only at 3' nucleotide position. Therefore, the amplified products from resistant and susceptible parasites were produced 267 and 266 bp, respectively. A total of 162 parasites were genotyped, of which 130 parasites found homozygous resistant 'rr', 22 heterozygous 'rS' and 10 homozygous susceptible 'SS' type. The prevalence of 'rr' individuals was higher in Jaipur (98%) followed by Avikanagar (93%) and Bikaner (50%) regions. Overall, the prevalence of BZ resistant allele (r) was higher (87%) as compared to 13% of BZ susceptible allele (S).

      • KCI등재

        Evaluation of in vitro ruminal fermentation of ensiled fruit byproducts and their potential for feed use

        Shimaa A Mousa,Pradeep K. Malik,Atul P. Kolte,Raghavendra Bhatta,Shigemitsu Kasuga,Yutaka Uyeno 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.1

        Objective: Ensiling of tannin-rich fruit byproducts (FB) involves quantitative and qualitative changes in the tannins, which would consequently change the rumen fermentation characteristics. This study aimed to evaluate whether ensiled FBs are effective in mitigating methane emission from ruminants by conducting in vitro assessments. Methods: Fruit byproducts (grape pomace, wild grape pomace, and persimmon skin) were collected and subjected to four-week ensiling by Lactobacillus buchneri inoculant. A defined feed component with or without FB samples (both fresh and ensiled material) were subjected to in vitro anaerobic culturing using rumen fluid sampled from beef cattle, and the fermentation parameters and microbial populations were monitored. Results: Reduced methane production and a proportional change in total volatile fatty acids (especially enhanced propionate proportion) was noted in bottles containing the FBs compared with that in the control (without FB). In addition, we found lower gene copy number of archaeal 16S rRNA and considerably higher levels of one of the major fibrolytic bacteria (Fibrobacter succinogenes) in the bottles containing FBs than in the control, particularly, when it was included in a forage-based feed. However, in the following cultivation experiment, we observed that FBs failed to exhibit a significant difference in methane production with or without polyethylene glycol, implying that tannins in the FBs may not be responsible for the mitigation of methane generation. Conclusion: The results of the in vitro cultivation experiments indicated that not only the composition but also ensiling of FBs affected rumen fermentation patterns and the degree of methane generation. This is primarily because of the compositional changes in the fibrous fraction during ensiling as well as the presence of readily fermented substrates, whereas tannins in these FBs seemed to have little effect on the ruminal fermentation kinetics.

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