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Kiyun Park(박기연),Won-Seok Kim(김원석),Ihn-Sil Kwak(곽인실) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5
Cadmium (Cd) pollution has been a noticeable issue in aquatic ecosystems due to increasing environmental concentrations of Cd resulting from industrial and human activities. In aquatic ecosystems, elevated temperatures influence physiological and reproductive mechanisms in fish. To investigate the effect of an increasing temperature on Cd toxicity, we analyzed the combined effects of Cd toxicity and temperature (rearing temperature of 26°C and heat stress at 34 °C) on antioxidant process of zebrafish (Danio rerio) embryos. The survival rate of zebrafish embryos decreased at relatively high Cd concentrations of 0.07 and 0.1 mg L<sup>-1</sup>. Abnormal morphology was induced by exposure to a combination of three concentrations of Cd exposure and increasing temperature. Cd exposure induced reactive oxygen species (ROS) production and cell death in the live zebrafish. High temperature (34 °C) triggered Cd-induced intracellular ROS production to a greater extent than the rearing temperature of 26 °C. Transcriptional levels of CAT and SOD genes were investigated. The mRNA expression of CAT and SOD, molecular indicators of oxidative stress, was increased significantly at 34 °C after Cd exposure. The mRNA expression of CAT was more sensitive to temperature than that of SOD in Cd-treated zebrafish. Finally, these results suggest that increasing temperature boosted the oxidative damages by Cd toxicity in the transcriptional regulation of genes involved in the antioxidant and detoxificant process.
Kiyun Park,Jung Sick Lee,Ihn-Sil Kwak1 전남대학교 수산과학연구소 2016 수산과학연구소논문집 Vol.24 No.-
Heat shock proteins (HSPs) production in cell is inducible by many physical and chemical stressors, providing adaptive significance for organisms when faced with environmental changes. In this study, we investigated transcriptional expressions of heat shock protein 26 (HSP26) by different temperature stress (20, 22, 24, 26, and 28°C) on Haliotis discus hannai, an important marine gastropod. After 12 h temperature exposure, HSP26 gene expression generally down-regulated in H. discus hannai exposed to all temperatures (p <0.05), in compare to 20°C control. Molecular response of HSP26 also significantly decreased in H. discus hannai exposed to increasing temperature for 48 h. In addition, transcriptional level of HSP26 mRNA was dramatically decreased as 5-fold or more in exposure condition of the relative high temperature (26 and 28°C) for 72 h. The down-regulation observed as temperature-dependent manner. These results suggest sensitive response of HSP26 gene could be used as a molecular marker to estimate stress condition in H. discus hannai abalones following increasing temperature.