Transcriptional response of heat shock protein 26 on the Pacific abalone Haliotis discus hannai following increased temperature stress

Kiyun Park,Jung Sick Lee,Ihn-Sil Kwak1 전남대학교 수산과학연구소 2016 수산과학연구소논문집 Vol.24 No.-

Heat shock proteins (HSPs) production in cell is inducible by many physical and chemical stressors, providing adaptive significance for organisms when faced with environmental changes. In this study, we investigated transcriptional expressions of heat shock protein 26 (HSP26) by different temperature stress (20, 22, 24, 26, and 28°C) on Haliotis discus hannai, an important marine gastropod. After 12 h temperature exposure, HSP26 gene expression generally down-regulated in H. discus hannai exposed to all temperatures (p <0.05), in compare to 20°C control. Molecular response of HSP26 also significantly decreased in H. discus hannai exposed to increasing temperature for 48 h. In addition, transcriptional level of HSP26 mRNA was dramatically decreased as 5-fold or more in exposure condition of the relative high temperature (26 and 28°C) for 72 h. The down-regulation observed as temperature-dependent manner. These results suggest sensitive response of HSP26 gene could be used as a molecular marker to estimate stress condition in H. discus hannai abalones following increasing temperature.

Aquatic Ecological Health and Characteristics of Chironomids Outflow Accidents in Tap Water

Ihn-Sil Kwak(곽인실) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5

In 2020, the chironomids larvae spill from activated carbon at a water purification plant occurred. The Chironomus(Diptera: Chironomidae) is a freshwater benthic invertebrate that is an dominant organism used for environmental pollution and water quality monitoring. In this study, we performed morphological classification and genetic species identification using the cytochrome c oxidase subunit I (COI) gene on mitochondrial DNA for an accurate species classification of Chironomus larvae found in tap water purification plants in species, including twelve Chironomus kiiensis, six Chironomus flaviplumus, one Chironomus dorsalis, and one Polypedilum yongsanensis (not included Genus Chironomus) in Incheon. mentum, the mandible, the antenna, and the claw. Based on the COI sequences of 21 individuals of 17 Chironomus species registered in NCBI Genbank, phylogenetic analysis indicated that the 20 individuals investigated in this study consist of the same clade with corresponding species of the high homology (99~100%) including C. kiiensis, C. flaviplumus, C. dorsalis, and P. yongsanensis. These results will be used as main classification indicator for monitoring freshwater ecosystems by providing integrated morphological and genetic information for the species identification of Korean Chironomus.

Changes in exoskeleton surface roughness and expression of chitinase genes in mud crab <i>Macrophthalmus japonicus</i> following heavy metal differences of estuary

Park, Kiyun,Kwak, Tae-Soo,Kim, Won-Seok,Kwak, Ihn-Sil Elsevier 2019 MARINE POLLUTION BULLETIN Vol.138 No.-

<P><B>Abstract</B></P> <P>Risk assessment of heavy metals is important for the health evaluation of inhabiting species in aquatic ecosystem. This study investigated whether chitin exoskeleton of mud crab <I>Macrophthalmus japonicus</I> is affected by heavy metals in estuary sediments in Korea. We compared heavy metal concentrations and analyzed the expression of <I>M. japonicus</I> chitinase genes, which play the crucial role in the formation of chitin exoskeleton. Concentrations of heavy metals were highly observed in crab body inhabiting Hampyeong among estuarine sites. High expressions of chitinase 1 were observed in crab gill and hepatopancreas from Myodo, which is the site with the lowest concentration of heavy metal in crab body. The surface roughness of the exoskeleton decreased with the increased concentration of heavy metals accumulated in the crab body. These results suggest that the total bioconcentration of heavy metals in crabs affected the expression of chitinase genes and changes in the exoskeleton surface roughness.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Concentrations of toxic heavy metals were highly observed in Hampyeong crab body. </LI> <LI> Chitinase 1 gene was prominently expressed in gills of <I>Macrophthalmus japonicus.</I> </LI> <LI> High expressions of chitinase 1 gene were observed in crab gill from Myodo site. </LI> <LI> Myodo is the site with the lowest concentration of heavy metal in crab body. </LI> <LI> The exoskeleton surface roughness decreased with the increased heavy metals of crabs. </LI> </UL> </P>

Salinity and bisphenol A alter cellular homeostasis and immune defense by heat shock proteins in the intertidal crab <i>Macrophthalmus japonicus</i>

Park, Kiyun,Kwak, Ihn-Sil Academic Press in association with the Estuarine a 2019 Estuarine, coastal and shelf science Vol.229 No.-

<P><B>Abstract</B></P> <P>Heat shock proteins (HSPs) play crucial roles in cellular metabolic processes, as well as in protective immune responses. In this study, we determined the cellular and immune-related functions of HSPs in the intertidal mud crab <I>Macrophthalmus japonicus</I> under osmotic and bisphenol A (BPA) exposure stress. We performed transcriptional profiling of six HSP genes (HSP70, HSP60, HSP90, HSP83, HSP40, and HSP21) in <I>M. japonicus</I> gills and hepatopancreas. Tissue-specific responses were observed as differentially expressed HSP transcripts in <I>M. japonicus</I>. Under osmotic stress, expression levels of HSP70, HSP90 and HSP40 mRNA increased at high salinity (40 psμ), and decreased at low salinity (10 psμ) in <I>M. japonicus</I> gills. In contrast, HSP60 expression was significantly diminished under high salinity, and increased under low salinity. HSP21 was down-regulated under all salinity changes over 1, 4, and 7 days. In the hepatopancreas, under osmotic stress, most HSP transcripts were up-regulated at an early exposure time point (day 1) with subsequent down-regulation (day 7). On day 4, HSP70, HSP60, HSP90 and HSP40 transcript levels increased in <I>M. japonicus</I>, whereas HSP83 and HSP21 transcript levels decreased in the hepatopancreas following most salinity changes. In addition, BPA exposure generally induced HSP responses in the gills. In particular, HSP70 and HSP83 were significantly up-regulated at all concentrations of BPA on days 1 and 4. However, induced responses of HSP transcripts diminished by day 7, except for HSP83 and HSP40. In the hepatopancreas, HSP70 and HSP90 mRNA expression showed significant up-regulation, whereas transcription of HSP60, HSP83, and HSP21 was down-regulated on days 1 and 4. These results suggested that HSPs are differently involved in the molecular adaptation and defense responses of crabs to BPA and osmotic stress.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Six HSPs gene responses of mud crab were quantified at osmotic and BPA stress. </LI> <LI> BPA exposure induced different expressions of HSPs following tissue type. </LI> <LI> Most HSPs showed up-regulation at high salinity change in <I>M. japonicus</I> crab. </LI> <LI> HSP90 and HSP83 expressed differently under salinity change and BPA exposure. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Characterization and gene expression of heat shock protein 90 in marine crab Charybdis japonica following bisphenol A and 4-nonylphenol exposures

Kiyun Park,Ihn-Sil Kwak 환경독성보건학회 2014 환경독성보건학회지 Vol.29 No.-

Objectives : Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone important in the maturation of a broad spectrum of protein. In this study, an HSP90 gene was isolated from Asian paddle crab, Charybdis japonica, as a bio-indicator to monitor the marine ecosystem. Methods : This work reports the responses of C. japonica HSP90 mRNA expression to cellular stress by endocrine disrupting chemicals (EDCs), such as bisphenol A (BPA) and 4-nonylphenol (NP) using real-time. reverse transcription polymerase chain reaction. Results : The deduced amino acid sequence of HSP90 from C. japonica shared a high degree of homology with their homologues in other species. In a phylogenetic analysis, C. japonica HSP90 is evolutionally related with an ortholog of the other crustacean species. The expression of HSP90 gene was almost distributed in all the examined tissues of the C. japonica crab but expression levels varied among the different body parts of the crabs. We examined HSP90 mRNA expression pattern in C. japonica crabs exposed to EDCs for various exposure times. The expression of HSP90 transcripts was significantly increased in C. japonica crabs exposed to BPA and NP at different concentrations for 12, 24, 48 and 96 hours. The mRNA expression of HSP90 gene was significantly induced in a concentration- and time-dependent manner after BPA or NP exposures for 96 hours. Conclusions : Taken together, expression analysis of Asian paddle crab HSP90 gene provided useful molecular information about crab responses in stress conditions and potential ways to monitor the EDCs stressors in marine environments.

Characterization and gene expression of heat shock protein 90 in marine crab Charybdis japonica following bisphenol A and 4-nonylphenol exposures

Park, Kiyun,Kwak, Ihn-Sil The Korean Society of Environmental Toxicology 2014 환경독성보건학회지 Vol.29 No.-

Objectives Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone important in the maturation of a broad spectrum of protein. In this study, an HSP90 gene was isolated from Asian paddle crab, Charybdis japonica, as a bio-indicator to monitor the marine ecosystem. Methods This work reports the responses of C. japonica HSP90 mRNA expression to cellular stress by endocrine disrupting chemicals (EDCs), such as bisphenol A (BPA) and 4-nonylphenol (NP) using real-time. reverse transcription polymerase chain reaction. Results The deduced amino acid sequence of HSP90 from C. japonica shared a high degree of homology with their homologues in other species. In a phylogenetic analysis, C. japonica HSP90 is evolutionally related with an ortholog of the other crustacean species. The expression of HSP90 gene was almost distributed in all the examined tissues of the C. japonica crab but expression levels varied among the different body parts of the crabs. We examined HSP90 mRNA expression pattern in C. japonica crabs exposed to EDCs for various exposure times. The expression of HSP90 transcripts was significantly increased in C. japonica crabs exposed to BPA and NP at different concentrations for 12, 24, 48 and 96 hours. The mRNA expression of HSP90 gene was significantly induced in a concentration- and time-dependent manner after BPA or NP exposures for 96 hours. Conclusions Taken together, expression analysis of Asian paddle crab HSP90 gene provided useful molecular information about crab responses in stress conditions and potential ways to monitor the EDCs stressors in marine environments.

EFFECT OF ENVIROMENTAL CHANGES ON THE EXPRESSION OF CHITINASE GENES IN AN INTERTIDAL MUD CRAB (Macrophthalmus japonicus)

Chamilani Nikapitiya,Ihn-Sil Kwak 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

Disrupting effects of antibiotic sulfathiazole on developmental process during sensitive life-cycle stage of <i>Chironomus riparius</i>

Park, Kiyun,Kwak, Ihn-Sil Elsevier 2018 CHEMOSPHERE - Vol.190 No.-

<P><B>Abstract</B></P> <P>Antibiotics in the environment are a concern due to their potential to harm humans and interrupt ecosystems. Sulfathiazole (STZ), a sulfonamide antibiotic, is commonly used in aquaculture and is typically found in aquatic ecosystems. We evaluated the ecological risk of STZ by examining biological, molecular and biochemical response in <I>Chironomus riparius.</I> Samples were exposed to STZ for 12, 24 and 96 h, and effects of STZ were evaluated at the molecular level by analyzing changes in gene expression related to the endocrine system, cellular stress response and enzyme activity of genes on antioxidant and detoxification pathways. STZ exposure induced significant effects on survival, growth and sex ratio of emergent adults and mouthpart deformity in <I>C. riparius</I>. STZ caused concentration and time-dependent toxicity in most of the selected biomarkers. STZ exposure leads to significant heat-shock response of protein genes (HSP70, HSP40, HSP90 and HSP27) and to disruption by up-regulating selected genes, including the ecdysone receptor gene, estrogen-related receptors, ultraspiracle and E74 early ecdysone-responsive gene. Furthermore, STZ induced alteration of enzyme activities on antioxidant and detoxification responses (catalase, superoxide dismutase, glutathione peroxidase and peroxidase) in <I>C. riparius</I>. By inducing oxidative stress, antibiotic STZ disturbs the endocrine system and produces adverse effects in growth processes of invertebrates.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Antibiotic exposure alters biological and physiological responses in invertebrates. </LI> <LI> The STZ induces significant effects on sex balance of emergent <I>C. riparius</I> adults. </LI> <LI> STZ exposure cause the development of mouthpart deformity in aquatic midges. </LI> <LI> STZ causes a dose and time-dependent toxicity in most of the selected biomarkers. </LI> <LI> Endocrine disrupting effects observed in <I>C. riparius</I> exposed to STZ. </LI> </UL> </P>

Antioxidative-related Genes Expression Following Perfluorooctane Sulfonate (PFOS) Exposure in the Intertidal Mud Crab, Macrophthalmus japonicus

Park, Kiyun,Nikapitiya, Chamilani,Kwak, Tae-Soo,Kwak, Ihn-Sil Korean Ocean Research & Development Institute and 2015 OCEAN SCIENCE JOURNAL Vol.50 No.3

Vitellogenin gene response as potential indicated maker for endocrine distrupting chemicals exposure in the intertidal mud crab (Macrophthalmus japonicus)

Kiyun Park(박기연),Ihn-Sil Kwak(곽인실) 환경독성보건학회 2019 한국독성학회 심포지움 및 학술발표회 Vol.2019 No.4