냉동 제대혈 세포의 체외 증폭

김삼용,김철희,배광봉,김현수,박상준,김종숙,윤환중,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Background : Cord blood(CB), which has no HLA restriction, is an alternative to bone marrow for hematopoietic stem cell transplantation. The use of cord blood, however, is limited by the number of progenitor/stem cells necessary to reconstitute the older child or adult. Therefore, ex vivo expansion of CB could have tremendous impact on diverse clinical settings. We studied the ex vivo expansion of isolated population of CD34_(+) cells from cryopreserved CB cells. Methods : CD34 cells were isolated from cryopreserved CB mononuclear cells. Purified cells were cultured with various combinations of hematopoietic growth factors including erythropoietin(EPO), stem cell factor(SCF), granulocyte-colony-stimulating factor(G-CSF), gra-nulocyte, macrophage-colony-stimulating factor(GM-CSF), interleukin-1β(IL-1β), 1L-3, and IL-6. After 7, 10 or 14 days of culture, the fold increases of colony-forming unit- granu-locyte, macrophage(CFU-GM), burst-forming unit-erythroid(BFU-E), colony-forming unit-mix (CFU-Mix), and high proliferative potential colony-forming cell(HPP-CFC) were evaluated. Results : Ten-day culture with the combination of EPO, SCF, G-CSF, IL-1β, and IL-3 resulted in a median of 60-fold increase of CFU-GM, which was greater than those with the combinations of less than 5 growth factors. The addition of IL-6 or GM-CSF to this combination did not enhance CFU-GM expansion. Ten-day culture was significantly superior to 7-day culture for CFU-GM expansion. Prolongation of culture to 14 days, however, revealed decreased expansion of CFU-GM compared to 10 days. BFU-E and CFU-Mix were expanded to 2~5 folds in 7-day culture with the combination of EPO, SCF, and G-CSF. Further expansion was not achieved in 10-day culture and colonies disappeared in 14-day culture. HPP-CFC was expanded to a median of 7.5 folds in 7-day culture with the combination of EPO, SCF, G-CSF, IL-1β, IL-3, and IL-6. Neither 10-day or 14 day-culture enhanced expansion of HPP-CFU. Conclusion : Cryopreserved cord blood cells maintain ex vivo expansion potential. In our system, 10-day culture with the combination consisting of EPO, SCF, G-CSF, IL-1β, and IL-3 seems to be adequate for hematopoietic progenitor/stem cell expansion from cryopreserved cord blood cells.

치상 돌기 골절의 전방 경유 나사못 고정술 치료 성적

김인영,김수한,이정길,김태선,김재휴,이제혁,강삼석 대한신경외과학회 1997 Journal of Korean neurosurgical society Vol.26 No.9

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.

혈관모세포종의 임상 분석

김은성,강삼석,이정길,김태선,정신,김재휴,김수한,이제혁 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.1

혈관모세포종의 임상 분석

김은성,강삼석,이정길,김태선,정신,김재휴,김수한,이제혁 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.5

Discovery of urinary metabolomic biomarkers for early detection of acute kidney injury

Won, A Jin,Kim, Siwon,Kim, Yoon Gyoon,Kim, Kyu-Bong,Choi, Wahn Soo,Kacew, Sam,Kim, Kyeong Seok,Jung, Jee H.,Lee, Byung Mu,Kim, Suhkmann,Kim, Hyung Sik The Royal Society of Chemistry 2016 Molecular bioSystems Vol.12 No.1

<P>The discovery of new biomarkers for early detection of drug-induced acute kidney injury (AKI) is clinically important. In this study, sensitive metabolomic biomarkers identified in the urine of rats were used to detect cisplatin-induced AKI. Cisplatin (10 mg kg(-1), i.p.) was administered to Sprague-Dawley rats, which were subsequently euthanized after 1, 3 or 5 days. In cisplatin-treated rats, mild histopathological alterations were noted at day 1, and these changes were severe at days 3 and 5. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at days 3 and 5. The levels of new urinary protein-based biomarkers, including kidney injury molecule-1 (KIM-1), glutathione S-transferase-alpha (GST-alpha), tissue inhibitor of metalloproteinase-1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, neutrophil, neutrophil gelatinase-associated lipocalin (NGAL), and osteopontin, were significantly elevated at days 3 and 5. Among urinary metabolites, trigonelline and 3-indoxylsulfate (3-IS) levels were significantly decreased in urine collected from cisplatin-treated rats prior to histological kidney damage. However, carbon tetrachloride (CCl4), a hepatotoxicant, did not affect these urinary biomarkers. Trigonelline is closely associated with GSH depletion and results in insufficient antioxidant capacity against cisplatin-induced AKI. The predominant cisplatin-induced AKI marker appeared to be reduced in urinary 3-IS levels. Because 3-IS is predominantly excreted via active secretion in proximal tubules, a decrease is indicative of tubular damage. Further, urinary excretion of 3-IS levels was markedly reduced in patients with AKI compared to normal subjects. The area under the curve receiver operating characteristics (AUC-ROC) for 3-IS was higher than for SCr, BUN, lactate dehydrogenase (LDH), total protein, and glucose. Therefore, low urinary or high serum 3-IS levels may be more useful for early detection of AKI than conventional biomarkers.</P>

야간 혈압 변동과 좌심실 질량, 이완기 기능 및 QT 간격과의 관계

김수중,이병호,손일석,강흥선,조정휘,김권삼,송정상,배종화 대한간질학회 2003 대한간질학회지 Vol.9 No.1

일본의 직업교육·훈련제도의 특성과 최근의 변화

김삼수 한국노동경제학회 2003 勞動經濟論集 Vol.26 No.2

이 논문에서는 노동시장과의 관련하에서 일본의 직업교육훈련제도의 특징과 최근의 변화 양상 및 의의를 구명하였다. 분석 틀로서는 마스덴(D. Marsden)과 미야모토(宮本光鯖)의 고용제도 모형을 이용하였다. '지적숙련 논쟁'의 검토 등을 통해 고도성장기 및 오일쇼크기에 형성된 일본의 직업교육훈련제도의 본질적 특징이 '작업능력으로서의 숙련'이 제도화되지 않은 채 OJT 중심의 기업내 훈련을 통해 직능자격제도상의 내부 승진에 의해 어느 정도 높은 수준의 다기능공으로서 기능을 형성하는 데 있음을 지적하였다. 최근의 장기 불황하에서 이러한 내부노동시장은 커다란 도전을 받고 있는데 그 변화의 방향은 내부노동시장의 붕괴가 아니다. 확대되는 외부노동시장을 자격제도의 정비에 의해 직업별 노동시장으로 정비하고 그것에 의해 내부노동시장을 유지해 나가는 정책 방향인 것을 구체적인 정책분석을 통해 밝혔다. This paper investigates the characteristics of Japanese vocational education and training(VET) sy stem and its recent changes with regard to the labour market changes under t he long-term economic depression. The employment system model of Marsden(199 9) is used as an analytical framework. By the review of 'the debate of intellectu al skill', it is pointed out that the fundamental characteristic of Japanese VET system l ies in forming multi-functional workers by the internal promotion in connection with the OJT-focused enterprise training along the job-competency rank system. In this system 'the skill as work-competency' is not institutionalised. By the long- term depression this type of internal labour market(ILM) has been under serious changes. By the policy analyses it is made clear that the direction of its change is not toward the collapse of the ILM. A series of labor market and VET polici es does aim at sustaining the ILM by the introduction of the unified job quali fication system, which is expected to institutionalise the widening external labo r market into the craft-based labour market.

Polyester 신합섬 직물의 농색염색 및 대전방지가공에 관한 연구 (Ⅰ)

김동권,이주형,이난형,김삼수,조환 영남대학교 지역발전연구소 1996 새마을지역개발연구 Vol.18 No.-

The Dyeing processing, which can be a low-cost and easily application to production field was investigated by using some bathochromic agent and dyestuffs. The Δ L values and dye up-takes of threated several fabrics were investigated according to the dye concentration and pH of dyebath. The results were as follows : 1. The selection of bathochromic agent acts as important factor for improvment of bathochromic property of fabrics. 2. The pH of dyebath has a little effect on bathochromic effect. 3. In case of one bath treatment, the bathochromic agent has little effect on the improving bathochromic property of treated fabric. 4. The efficiency order of bathochromic effect is DTY + fila.> CVY > DTY and fabric structure is also effect on the bathochromic property of the polyester fabric.

이 심전도의 진단은?

김삼수 한국의학사 2004 診斷과治療 Vol.24 No.5