Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

Autophagy and mitochondrial damage in endothelial progenitor cells under high glucose condition

Ok-Nam Bae(배옥남),Kyeong-A Kim(김경아),Young-Jun Shin(신영준) 환경독성보건학회 2013 한국독성학회 심포지움 및 학술발표회 Vol.2013 No.10

Risk of metachronous neoplasia on surveillance colonoscopy in young patients with colorectal neoplasia

Kim, Hyun Gun,Cho, Young-Seok,Cha, Jae Myung,Shin, Jeong Eun,Kim, Kyeong Ok,Yang, Hyo-Joon,Koo, Hoon Sup,Joo, Young-Eun,Boo, Sun-Jin Elsevier 2018 Gastrointestinal Endoscopy Vol.87 No.3

<P><B>Background and Aims</B></P> <P>Few prior reports exist that address the appropriate colonoscopy surveillance interval for individuals <50 years old. We compared the risk of metachronous neoplasia between younger (20-49 years) and older (50-54 years) cohorts.</P> <P><B>Methods</B></P> <P>This multicenter retrospective cohort study compared the incidence of metachronous neoplasia in younger and older cohorts according to baseline risk stratification. Subjects were eligible if they underwent their first colonoscopy between June 2006 and May 2010 and had at least 1 or more surveillance colonoscopy up to June 2015.</P> <P><B>Results</B></P> <P>Among a total of 10,477 subjects who underwent baseline colonoscopy, 9722 were eligible after excluding 755 subjects. Of those 9722 subjects, 43% underwent surveillance colonoscopy. In the baseline high-risk adenoma group (n = 840), the 3-year risk of metachronous advanced neoplasia was 10.7% in the younger patients on screening colonoscopy and 8.9% in the older patients (<I>P</I> > .1). In the baseline low-risk adenoma group (n = 1869), the 5-year risk of metachronous advanced neoplasia was 4.9% in the younger patients on screening colonoscopy and 5.1% in the older patients (<I>P</I> > .1). Similarly, in the baseline no neoplasia group (n = 7013), the 5-year risk of metachronous advanced neoplasia was 4.1% in the younger patients on screening colonoscopy and 5.6% in the older patients (<I>P</I> > .1).</P> <P><B>Conclusions</B></P> <P>Considering the similar risk of metachronous advanced neoplasia in younger and older individuals, we suggest a 3-year surveillance interval for high-risk adenoma and a 5-year surveillance interval for low-risk adenoma in young individuals without a strong family history.</P>

Role of Autophagy in Endothelial Damage and Blood–Brain Barrier Disruption in Ischemic Stroke

Kim, Kyeong-A,Shin, Donggeun,Kim, Jeong-Hyeon,Shin, Young-Jun,Rajanikant, G.K.,Majid, Arshad,Baek, Seung-Hoon,Bae, Ok-Nam American Heart Association, Inc. 2018 Stroke Vol.49 No.6

<P>The global burden of neurological diseases including stroke has significantly increased, 1 and an urgent need exists to develop new treatment strategies. Impairment of autophagic regulation has been observed in diseases including neurodegenerative diseases and ischemic stroke, suggesting that modulation of autophagy could be a potential therapeutic target. 2 Endothelial cells (ECs) maintain homeostasis by regulating the vascular tone and permeability and endothelial dysfunction is associated with diverse cardiovascular diseases (CVDs). The integrity of the blood-brain barrier (BBB), which shows selective permeability for substances into the brain, is significantly impaired under ischemic stroke. This review focuses on autophagy in endothelial dysfunction in the context of ischemic stroke and potential targets for therapeutic manipulation.</P>

Reduced metabolic activity of gut microbiota by antibiotics can potentiate the antithrombotic effect of aspirin

Kim, In Sook,Yoo, Dae-Hyeong,Jung, Il-Hoon,Lim, Sumin,Jeong, Jin-Ju,Kim, Kyeong-A,Bae, Ok-Nam,Yoo, Hye Hyun,Kim, Dong-Hyun Elsevier 2016 Biochemical pharmacology Vol.122 No.-

<P><B>Abstract</B></P> <P>In this study, we investigated the effects of antibiotics on the pharmacological effects of aspirin. The antithrombotic activity of aspirin was evaluated after antibiotic treatment using tail bleeding assay. The pyrosequencing analysis and selective medium culture assay were performed to investigate the alterations in gut microbiota. In addition, the <I>in vitro</I> metabolism assay with fecal suspension and <I>in vivo</I> pharmacokinetic experiments with antibiotic treatment were conducted. Ampicillin treatment significantly prolonged the bleeding time in aspirin-dosed rats. Oral administration of ampicillin significantly reduced gut microbial aspirin-metabolizing activity by 67.0% in rats. Furthermore, systemic exposure to aspirin and its primary metabolite (M1) was significantly increased in ampicillin-treated rats. The results from the pyrosequencing and selective medium culture with rat fecal samples revealed that ampicillin treatment led to the changes of the amounts and composition profile of gut microbiota. These findings suggest that co-administration of antibiotics can modulate the metabolism and pharmacokinetics of aspirin via suppression of metabolic activity of gut microbiota, which could potentiate the therapeutic potency of aspirin.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Ginsenoside Re Ameliorates Brain Insulin Resistance and Cognitive Dysfunction in High Fat Diet-Induced C57BL/6 Mice

Kim, Jong Min,Park, Chang Hyeon,Park, Seon Kyeong,Seung, Tae Wan,Kang, Jin Yong,Ha, Jeong Su,Lee, Du Sang,Lee, Uk,Kim, Dae-Ok,Heo, Ho Jin American Chemical Society, Books and Journals Divi 2017 Journal of agricultural and food chemistry Vol.65 No.13

<P>The ameliorating effects of ginsenoside Re (G Re) on high fat diet (HFD)-induced insulin resistance in CS7BL/6 mice were investigated to assess its physiological function. In the results of behavioral tests, G Re improved cognitive dysfunction in diabetic mice using Y-maze, passive avoidance, and Morris water maze tests. G Re also significantly recovered hyperglycemia and fasting blood glucose level. In the results of serum analysis, G Re decreased triglyceride (TG), total cholesterol (TCHQ), low-density lipoprotein cholesterol (LDLC), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) and :increased the ratio of high-density lipoprotein cholesterol (HDLC). G Re regulated acetylcholine (ACh), acetylcholinesterase (AChE), malondialdehyde (MDA), superoxide dismutase (SOD), and oxidized glutathione (GSH)/total GSH by regtilating the c-Jun N-terminal protein kinase (JNK) pathway. These findings suggest that G Re could be used to improve HFD-induced insulin resistance condition by ameliorating hyperglycemia via protecting the cholinergic and antioxidant systems in the mouse brains.</P>

Ichthyofauna and structure of fish community in Wangpi Stream, Korea

Kyeong-Hwan Kim,Kyengmu Kim,Kwangho Lim,Yang-Ki Hong,Mi-Young Song,Wan-Ok Lee 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.10

Propagation of a tsunami wave generated by an earthquake in the Nankai Trough onto the South Korean coast

Kim, Kyeong Ok,Jung, Kyung Tae,Choi, Byung Ho BioOne (Coastal Education and Research Foundation) 2013 JOURNAL OF COASTAL RESEARCH Vol.65 No.-

<P>The earthquake of magnitude 9.0 that occurred in March 2011 and the ensuing crisis at the Fukushima Daiichi nuclear power plant prompted sweeping reviews of Japan's disaster preparedness and criticism over apparent failures to take into account potential risks. Based on studies conducted after the catastrophes, the Japanese government revised its tsunami projections in a report posted on a government website. While the earlier forecast in 2003 put the potential maximum height of a tsunami runup at less than 20 m, a governmental panel of experts in Japan recently reported that the occurrence of a magnitude-9.0 earthquake in the Nankai trough, which runs east of Japan's main island of Honshu to the southern island of Kyushu, might generate a tsunami runup with a height of more than 34 m, which would inundate much of Japan's Pacific coast. A chronicle of Cheju Island (Tamraji) reports that a tsunami was propagated by the 1707 Hoei earthquake. The tsunami from the 2011 earthquake event was also propagated to the southern shore of Korea. In this study, we conducted a numerical simulation of the propagation of the tsunami from Japan's Pacific coast to the southern shore of the Korean Peninsula. Simulation results are presented for 11 scenarios of initial water displacement identified by the Japanese government. The simulation results indicate that after an earthquake in the Nankai Trough, tsunami waves would reach Cheju Island in approximately 3.5 hours and the southern coast of Korea in approximately 4 hours, with a maximum runup height of approximately 1.5 m.</P>

S-453 CORONARY ARTERY CALCIFICATION PREDICTS MICROCALCIFICAION OF VASCULAR ACCESS IN HEMODIALYSIS PATIENTS

( Kyeong Pyo Lee ),( Bomi Choi ),( Young Soo Kim ),( Sun Ae Yoon ),( Young Ok Kim ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Background:?We have reported that arterial micro-calcification (AMC) of vascular access has a negative impact on access patency and cardiovascular mortality in hemodialysis (HD) patients. Because AMC is diagnosed by histologic examination, it cannot be commonly performed. Coronary artery calcification (CAC) is quite common and known risk factors of cardiovascular mortality in HD patients. It can be easily detected by noninvasive computed tomography. Therefore we evaluated which CAC may predict AMC in nondiabetic HD patients, because we excluded impact of diabetes mellitus on CAC and AMC.?Methods:?Forty non diabetic incident HD patients who received vascular access operation were included in this study. The AMC was diagnosed by pathologic examination of arterial specimen by von Kossa stain, which was acquired during the operation. All patients underwent a multi-detector computed tomography (MDCT) imaging procedure and coronary artery calcium score (CACS) was calculated. Patients were classified into two groups, according to the CACS, as low (<100), in 23 patients, and high (≥100), in 17 patients. We compared CACS between the patients with and without AMC.?Results:Mean age was 63.4±16.5 years and the male gender was 24 (60.0%). The incidence of AMC was 35.0% (n=14). The mean CACS was 185.9±299.4 (0-1421.7), and the median value was 54.1. Patients with high CACS group were older than low CACS group(74.1±9.7 211vs 55.5±16.1, p<0.05). High CACS showed higher incidence of AMC compared to low CACS group (58.8% vs 17.4%, p<0.05). By binary logistic regression, high CACS was independently associated with positive AMC (OR 8.381, 95% CI 1.725-40.732, p<0.05).?Conclusions:?The present study suggests that CAC predicts AMC of vascular access in nondiabetic HD patients.

Endoscopic activity in inflammatory bowel disease: clinical significance and application in practice

Kyeong Ok Kim 대한소화기내시경학회 2022 Clinical Endoscopy Vol.55 No.4

Endoscopy is vital for diagnosis, assessing treatment response, monitoring and surveillance in patients with inflammatory bowel disease(IBD). With the growing importance of mucosal healing as a treatment target, the assessment of disease activity by endoscopy hasbeen accepted as the standard of care for IBD. There are many endoscopic activity indices for facilitating standardized reporting of thegastrointestinal mucosal appearance in IBD, and each index has its strengths and weaknesses. Although most endoscopic indices donot have a clear-cut validated definition, endoscopic remission or mucosal healing is associated with favorable outcomes, such as a decreasedrisk of relapse. Therefore, experts suggest utilizing endoscopic indices for monitoring disease activity and optimizing treatmentto achieve remission. However, the regular monitoring of endoscopic activity is limited in practice owing to several factors, such as thecomplexity of the procedure, time consumption, inter-observer variability, and lack of a clear-cut, validated definition of endoscopicresponse or remission. Although experts have recently suggested consensus-based definitions, further studies are needed to define thevalues that can predict long-term outcomes.