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김갑식(Kab Sig Kim),손종현(Jong Hyeoun Son),최광태(Kwang Tae Choi) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.2
In order to characterize the chloroplasts of Korean ginseng as a semi-shade plant and radish as a sun plant, effects of growth light intensity on photosynthetic electron transport (PS) activity in chloroplasts and superoxide (O_2·^-) production in thylakoid membrane by irradiation were investigated. High-light chloroplasts of both plants showed higher PS activities than those grown under low growth light intensity. High PSⅡ and low PSⅠ activities in ginseng chloroplasts (ratio of PSⅡ/PSⅠ:1.1) were observed, but radish chloroplasts showed low PSⅡ and high PSⅠ activities (ratio of PSⅡ/PSⅠ:0.3). PSⅡ activity of both plants was little affected by temperature in ranges of 15-35℃. Activities of whole-chain (PSⅡ+Ⅰ) in ginseng and PSⅠ in radish were increased at high temperature (40℃). Preincubation of chloroplasts at 40℃ during 30 min, as a mild heat stress, caused rapid decrease in PSⅡ and PSⅡ+Ⅰ activities of both plants. However PSⅠ activity was not decreased in ginseng and rather increased in radish. O_2·^- production (NBT reduction) in Mehler reaction in the thylakoid membrane was inhibited by DCMU in both plants. DMBIB inhibited O_2·^- production in ginseng, but radish was insensitive to DMBIB. Electron flow system in ginseng thylakoid membrane was more susceptible to damage of photooxidation than that of radish.
세균 Stenotrophomonas sp. KTGBP10의 식물 바이러스 감염억제효과
김영숙,황의일,오정훈,김갑식,여운형,Kim Young-Sook,Hwang Eui-Ii,Oh Jung-Hoon,Kim Kab-Sig,Yeo Woon-Hyung 한국연초학회 2004 한국연초학회지 Vol.26 No.2
During the screening of antiviral substances having inhibitory effects on tobacco mosaic virus (TMV) infection to tobacco plants, we found a bacterial isolate KTGBP10, which was identified as a Stenotrophomonas sp., strongly inhibited the infection of TMV. When the culture filtrate from KTGBP10 was applied on the upper surface of leaves of Xanthi-nc tobacco plants at the same time or 24 hours before TMV inoculation, almost complete inhibition of TMV infection was achieved. And $40\%$ inhibition was shown with application of the culture filtrate to the under surface of leaves. In field trials, transmission of TMV from diseased seedlings to the healthy ones during transplanting work was reduced by $87.1\~92.6\%$ when the culture filtrate or cell suspension was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. When the broth filtrate of KTGBP10 was supplied by soaking through the cut-leaves before and/or after virus inoculation, the TMV infection was also inhibited by $50.4\~65.3\%$.
Park, Ui Sun,Choi, Kwan Sam,Kim, Kab-Sig,Kim, Nam Won,Choi, Kwang-Tae 충남대학교 생물공학연구소 1996 생물공학연구지 Vol.4 No.-
미트콘드리아내 유전자는 염기서열 재배치에 의해 단일 또는 여러 copy로 존재하는데 그 발현정도에 따라 생리적 특이 현상을 나타낼 것으로 생각되어 세포질 유전의 웅성불임현상에 대한 연구가 활발히 이루어져 왔다. 반음지성이며 생육이 느린 고려인삼(Panaxginseng C. A. Meye)의 특성을 유전자 수준에서 접근하기 위해 F_1ATPase α-subunit 유전자(aptA에 대해 실험하였다. 고려인삼에는 atpA 유전자가 미토콘드리아 DNA에 단일 copy로 존재함을 확인하였으며, 미토콘드리아 DNA의 SalI 및 PstI 유전자은행으로부터 Oenothera atpA유전자와 homology를 보이는 clony을 clone lift hybridization에 의해 선발하였는데, 각각 3.4 kb와 13 kb의 미토콘드리아 DNA조각을 함유하고 있었다. 이 Sal I clone과 Pst I clone에 대해 Southern blot hybridization과 부분적인 염기서열분석을 실시한 결과, 삽입된 3.4 kb와 13 kb의 미토콘드리아 DNA조각은 Oenothera, maize, pea, Phaseolus vulgaris의 atpA유전자와 homology를 보이는 sequence를 포함하고 있었다. 타 식물의 atpA 유전자의 coding sequence와 비교해 볼때, Sal I clone은 initiation codon으로부터 약 300 bp를 제외한 atpA유전자의 나머지 약 1.2 kb sequence를 포함하고 있고, Pst I clone은 atpA유전자의 완전한 coding sequence를 포함하고 있는 것으로 여겨진다. 이들 두 clone의 제한효소 지도와 부분적인 염기서열이 제시되었다. 고려인삼은 단일 copy의 atpA유전자를 갖고있기 때문에 이 타 식물에서와 같이 복잡한 유전자 재배치에 따른 생리적 불균형의 가능성은 없는 것으로 사료된다. Molecular cloning and restriction mapping on ATPase α-subunit gene (atpA) were carried out to obtain genomic information concerned with the gene structure and organization in Korean ginseng mitochondria. Two different clones containing the homologous sequence of atpA gene were selected from SalI and PstI libraries of mitochondrial DNA (mtDNA) of Korean ginseng. The sizes of mtDNA fragments inserted in SalI and PstI clones were 3.4 kb and 13 kb, respectively. Southern blot analysis with [^32P]labelled Oenothera atpA gene probe showed that atpA gene sequence was located in 2.0 kb XbaI fragment in PstI clone and in 1.7 kb XbaI fragment in SalI clone. A partial sequencing ascertained that the SalI clone included about 1.2 kb fragment from SalI restriction site to C-terminal sequence of this gene but about 0.3 kb N-terminal sequence of open reading frame was abscent. The PstI fragment was enough large to cover the full sequence of atpA gene. The same restriction pattern of the overlapped region suggests that both clones include the same fragment of atpA locus. Date of Southern blot analysis and partial nucleotide sequencing suggested that mtDNA of Korean ginseng has a single copy atpA gene.