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도시경관에 있어서 높이기준유형별 건축물의 형태적 특징에 관한 연구
김도년,임희지 대한건축학회 2004 대한건축학회논문집 Vol.20 No.8
Height control is about providing light and air on the streets and managing urban cityscape In order to achieve these goals, major cities in foreign countries are administering various unique height control methods that are adaptable to the city And by examining the architectural characteristics of the cities with various height control methods the following conclusions have been made In most modern cities excluding European historical cities paris and Berlin height limits are eased and controlled by each lot based on the widths of the adjacent streets There is a tendency to lighten the height limits as the height is controlled improvement as it interrupts the continuity of streets results in excessive tall towers and produces abnormal form of buildings like slanted facets Cities like New York and Tokyo, which previously controlled the height by lot are now adopting height-controlling by district in order to create unified street landscapes Recently seoul has introduced block height limits a form of district height controlling methods but it seems that there is a need for various height limits in the areas of atypical urban tissue and of unique characteristics so that they can be applied to the whole city.
지역경제 활성화를 위한 환경정비형 지구단위계획의 시행평가 연구
배웅규,김도년 대한건축학회 2005 대한건축학회논문집 Vol.21 No.2
The purpose of this study is to propose new District Unit Plan approach to meet changes in social and urban circumstances; existing built-up areas and the need for business improvement in the process of urban development. The paper's significance lies in that it shows a new direction in urban design which has been made into a district unit plan through in support of the application to the pilot project compared with previous physical and business environment. The proposed urban design technique for the improvement of existing commercial areas, specially commercial streets have advantages. Local Government' initiation makes possible to make improvement and give administrative and financial support which is sensitive to the existing conditions of urban area for the better environment. Also it can maintain and improve the vitality of existing retail street by taking into account it's potentiality. This new application can maximize not only the effect of improvement of existing commercial district but also the build of community board can be mutual communication channer of public sector. Thus, this research suggests how district unit plan as a newly organized urban design system can cope with collaborating a community board and a local government.
Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1
Kim, Do Nyun,Seo, Min Koo,Choi, Hoyun,Kim, Su Yeon,Shin, Hee Jong,Yoon, A-Ran,Tao, Qian,Rha, Sun Young,Lee, Suk Kyeong Microbiology Society 2013 The Journal of general virology Vol.94 No.3
<P>Epstein-Barr virus (EBV) is a herpesvirus associated with lymphomas and carcinomas. While EBV-associated epithelial cell lines are good model systems to investigate the role of EBV in carcinoma, only a few cell lines are available as they are hard to acquire. A greater variety of naturally EBV-infected cell lines which are derived from tumour patients are needed to represent various features of EBVaGC. We characterized cell line YCCEL1, established from a Korean EBVaGC patient, to ascertain whether it can be used to study the roles of EBV in EBVaGC. The expression of EBV genes and cell surface markers was examined by in situ hybridization, RT-PCR, Western blot analysis, immunofluorescence assay and Northern blot analysis. EBV episomal status was analysed by Southern blotting and real-time PCR. This cell line expressed EBV nuclear antigen 1 (EBNA1) and latent membrane protein 2A (LMP2A), but not EBNA2, LMP2B nor LMP1. The majority of the lytic proteins were not detected in YCCEL1 cells either before or after treatment with 12-O-tetradecanoylphorbol-13-acetate. YCCEL1 cells expressed BART microRNAs (miRNAs) at high level but did not express BHRF1 miRNAs. YCCEL1 cells expressed cytokeratin, but not CD21 and CD19, suggesting CD21-independent EBV infection. The latent EBV gene and EBV miRNA expression pattern of YCCEL1 cells closely resembled that of general EBVaGC cases. Our results support the value of YCCEL1 cells as a good model system to study the role of EBV in gastric carcinogenesis.</P>
Usefulness of FibroScan for Detection of Early Compensated Liver Cirrhosis in Chronic Hepatitis B
Kim, Do Young,Kim, Seung Up,Ahn, Sang Hoon,Park, Jun Yong,Lee, Jung Min,Park, Young Nyun,Yoon, Ki Tae,Paik, Yong Han,Lee, Kwan Sik,Chon, Chae Yoon,Han, Kwang-Hyub Springer-Verlag 2009 Digestive diseases and sciences Vol.54 No.8
Biogenesis of Epstein-Barr virus microRNAs.
Kim, Do Nyun,Lee, Suk Kyeong Dr. W. Junk B. V. Publishers ; Kluwer Academic Pub 2012 MOLECULAR AND CELLULAR BIOCHEMISTRY - Vol.365 No.1
<P>Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus implicated in lymphomas, such as Burkitt's lymphoma, Hodgkin's lymphoma, and NK/T cell lymphoma. MicroRNAs (miRNAs) are 19-25 nucleotide long single-stranded RNAs involved in post-transcriptional gene regulation. miRNAs are mainly transcribed by RNA polymerase II (pol II) to have stem-loop structures and subsequently processed by Drosha and Dicer. EBV miRNAs are expressed in B cells, nasopharyngeal carcinoma cells, and gastric carcinoma cells infected with EBV. EBV miRNAs can be divided into two groups: BHRF1 miRNAs and BART miRNAs. In this study, we investigated the biogenesis of EBV miRNAs. Treatment of the SNU-719 EBV-positive gastric cancer cell line with 관-amanitin at a concentration that selectively inhibits RNA polymerase II activity decreased the expression levels of BART miRNAs. The expression levels of BART miRNAs were also reduced by RNA interference targeting Drosha and Dicer. Two of each C/EBP관 and c-Myc binding sites are located upstream of the proposed initiation sites for primary BART miRNA transcripts. Knockdown of C/EBP관 but not c-Myc using siRNAs reduced BART miRNA expression by 25-55% compared with the control. These results suggest that BART miRNAs are transcribed by pol II and undergo a similar biogenesis process with cellular miRNAs.</P>
Kim, Do-Nyun,Song, Yoon-Jae,Lee, Suk-Kyeong Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.7
Epstein-Barr virus (EBV) microRNAs (miRNAs) are expressed in EBV-associated tumors and cell lines, but the regulation mechanism of their expression is unclear yet. We investigated whether the expression of EBV miRNAs is epigenetically regulated in EBV-infected B cell lines. The expression of BART miRNAs was inversely related with the methylation level of the BART promoter at both steady-state and following 5-aza-2'-deoxycytidine treatment of the cells. The expression of BHRF1 miRNAs also became detectable with the demethylation of Cp/Wp in latency I EBV-infected cell lines. Furthermore, $in$ $vitro$ methylation of the BART and Cp promoters reduced the promoter-driven transactivation. In contrast, tricostatin A had little effect on the expression of EBV miRNA expression as well as on the BART and Cp/Wp promoters. Our results suggest that promoter methylation, but not histone acetylation, plays a role in regulation of the EBV miRNA expression in EBV-infected B cell lines.
Expression of Viral MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma
Kim, Do Nyun,Chae, Hiun-Suk,Oh, Sang Taek,Kang, Jin-Hyoung,Park, Cho Hyun,Park, Won Sang,Takada, Kenzo,Lee, Jae Myun,Lee, Won-Keun,Lee, Suk Kyeong American Society for Microbiology 2007 Journal of virology Vol.81 No.2
<B>ABSTRACT</B><P>Epstein-Barr virus (EBV) is associated with about 6 to 16% of gastric carcinoma cases worldwide. Expression of the EBV microRNAs (miRNAs) was observed in B cells and nasopharyngeal carcinoma cells infected with EBV. However, it is not clear if the EBV miRNAs are expressed in EBV-associated gastric carcinomas (EBVaGCs). We found that BART miRNAs but not BHRF1 miRNAs were expressed in EBV-infected gastric carcinoma cell lines and the tumor tissues from patients as well as the animal model. The expression of viral miRNAs in EBVaGCs suggests that these EBV miRNAs may play important roles in the tumorigenesis of EBVaGCs.</P>